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- Du, Guan-Hua, et al. Show all 8 Authors
- Biotechnology letters 2009 v.31 no.7 pp. 979-984
- Pichia pastoris; genes; human cell lines; humans; inhibitory concentration 50; noninsulin-dependent diabetes mellitus; screening
- ... Dipeptidyl peptidase (DPP) IV inhibitors provide a new strategy for the treatment of type 2 diabetes. Human DPP-IV gene was cloned from differentiated Caco-2 cells and expressed in Pichia pastoris. The recombinant enzyme was used in a new system for screening of DPP-IV inhibitors. By high throughput screening, a novel compound (W5188) was identified from 75,000 compounds with an IC₅₀ of 6.5 μM. Th ...