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41. Indole and 7-benzyloxyindole attenuate the virulence of Staphylococcus aureus

Author:
Lee, Jintae,   et al.   ; Lee, Jin-Hyung; Cho, Hyun Seob; Kim, Younghoon; Kim, Jung-Ae; Banskota, Suhrid; Cho, Moo Hwan;     Show all 7 Authors
Source:
Applied microbiology and biotechnology 2013 v.97 no.10 pp. 4543-4552
ISSN:
0175-7598
Subject:
Caenorhabditis elegans; Staphylococcus aureus; antibiotics; blood; carotenoids; cross infection; digestive system; drug resistance; hemolysis; hosts; humans; hydrogen peroxide; immune system; microbial growth; pathogens; proteinases; regulator genes; transcription (genetics); viability; virulence
Abstract:
... Human pathogens can readily develop drug resistance due to the long-term use of antibiotics that mostly inhibit bacterial growth. Unlike antibiotics, antivirulence compounds diminish bacterial virulence without affecting cell viability and thus, may not lead to drug resistance. Staphylococcus aureus is a major agent of nosocomial infections and produces diverse virulence factors, such as the yello ...
DOI:
10.1007/s00253-012-4674-z
PubMed:
23318836

42. Reconfiguring the Quorum-Sensing Regulator SdiA of Escherichia coli To Control Biofilm Formation via Indole and N-Acylhomoserine Lactones

Author:
Lee, Jintae,   et al.   ; Maeda, Toshinari; Hong, Seok Hoon; Wood, Thomas K.;     Show all 4 Authors
Source:
Applied and environmental microbiology 2009 v.75 no.6 pp. 1703-1716
ISSN:
0099-2240
Subject:
metabolism; cell movement; lactones; biofilm; tryptophan 2,3-dioxygenase; microarray technology; quorum sensing; mutation; swimming; amino acids; genes; protein engineering; bacteria; Escherichia coli
Abstract:
... SdiA is a homolog of quorum-sensing regulators that detects N-acylhomoserine lactone (AHL) signals from other bacteria. Escherichia coli uses SdiA to reduce its biofilm formation in the presence of both AHLs and its own signal indole. Here we reconfigured SdiA (240 amino acids) to control biofilm formation using protein engineering. Four SdiA variants were obtained with altered biofilm formation, ...
DOI:
10.1128/AEM.02081-08

43. YliH (BssR) and YceP (BssS) Regulate Escherichia coli K-12 Biofilm Formation by Influencing Cell Signaling

Author:
Lee, Jintae,   et al.   ; Domka, Joanna; Wood, Thomas K.;     Show all 3 Authors
Source:
Applied and environmental microbiology 2006 v.72 no.4 pp. 2449-2459
ISSN:
0099-2240
Subject:
Escherichia coli; amino acids; biofilm; flagellum; gene expression; genes; glucose; growing media; loci; molecular motor proteins; mutants; nomen novum; phenotype; protein transport; quorum sensing; secretion; stress response; wood
Abstract:
... We previously discovered that yliH and yceP are induced in Escherichia coli biofilms (D. Ren, L. A. Bedzyk, S. M. Thomas, R. W. Ye, and T. K. Wood, Appl. Microbiol. Biotechnol. 64:515-524, 2004). Here, it is shown that deletion of yceP (b1060) and yliH (b0836) increases biofilm formation in continuous-flow chambers with minimal glucose medium by increasing biofilm mass (240- to 290-fold), surface ...
DOI:
10.1128/AEM.72.4.2449-2459.2006
PubMed:
16597943
PubMed Central:
PMC1448992

44. Transcriptomic Analysis for Genetic Mechanisms of the Factors Related to Biofilm Formation in Escherichia coli O157:H7

Author:
Lee, Jintae,   et al.   ; Lee, Jin-Hyung; Kim, Yong-Guy; Cho, Moo Hwan; Wood, Thomas K.;     Show all 5 Authors
Source:
Current microbiology 2011 v.62 no.4 pp. 1321-1330
ISSN:
0343-8651
Subject:
Escherichia coli O157; bacteriophages; biofilm; biosynthesis; cellulose; genes; microarray technology; polyethylene; scanning electron microscopy; transcriptomics
Abstract:
... Two lineages of enterohemorrhagic Escherichia coli O157:H7 (EDL933, Stx1+ and Stx2+) and 86-24 (Stx2+) were investigated to determine the genetic basis of biofilm formation on abiotic surfaces. Strain EDL933 formed a robust biofilm while strain 86-24 formed almost no biofilm on either polystyrene plates or polyethylene tubes. Whole-transcriptome profiles of EDL933 versus 86-24 revealed that in the ...
DOI:
10.1007/s00284-010-9862-4
PubMed:
21221972
PubMed Central:
PMC3158427

45. Environmental factors affecting indole production in Escherichia coli

Author:
Lee, Jintae,   et al.   ; Han, Thi Hiep; Lee, Jin-Hyung; Cho, Moo Hwan; Wood, Thomas K.;     Show all 5 Authors
Source:
Research in microbiology 2011 v.162 no.2 pp. 108-116
ISSN:
0923-2508
Subject:
Escherichia coli; Gram-negative bacteria; amino acids; ampicillin; biofilm; biosynthesis; carbon; cell viability; environmental factors; kanamycin; microbial communities; pH; temperature
Abstract:
... A variety of both Gram-positive and Gram-negative bacteria produce large quantities of indole as an intercellular signal in microbial communities. Biosynthesis of indole is well-studied, and while carbon sources and amino acids are important environmental cues for indole production in Escherichia coli, other environmental factors affecting indole production for this strain are less clear. This stu ...
DOI:
10.1016/j.resmic.2010.11.005
PubMed:
21145393
PubMed Central:
PMC3171796

46. Antibiofilm activity of Streptomyces sp. BFI 230 and Kribbella sp. BFI 1562 against Pseudomonas aeruginosa

Author:
Lee, Jintae,   et al.   ; Kim, Yong-Guy; Lee, Jin-Hyung; Kim, Chang-Jin; Lee, Jae-Chan; Ju, Yoon Jung; Cho, Moo Hwan;     Show all 7 Authors
Source:
Applied microbiology and biotechnology 2012 v.96 no.6 pp. 1607-1617
ISSN:
0175-7598
Subject:
Pseudomonas aeruginosa; Streptomyces; antibiotics; bioactive properties; biofilm; culture media; genes; heat treatment; iron; peptides; proteins; screening; swarming; transcriptomics
Abstract:
... Members of the actinomycetes family are a rich source of bioactive compounds including diverse antibiotics. This study sought to identify novel and non-toxic biofilm inhibitors from the actinomycetes library for reducing the biofilm formation of Pseudomonas aeruginosa PAO1. After the screening of 4104 actinomycetes strains, we found that the culture spent medium (1 %, v/v) of Streptomyces sp. BFI ...
DOI:
10.1007/s00253-012-4225-7
PubMed:
22722911

47. Indole-3-acetaldehyde from Rhodococcus sp. BFI 332 inhibits Escherichia coli O157:H7 biofilm formation

Author:
Lee, Jintae,   et al.   ; Lee, Jin-Hyung; Kim, Yong-Guy; Kim, Chang-Jin; Lee, Jae-Chan; Cho, Moo Hwan;     Show all 6 Authors
Source:
Applied microbiology and biotechnology 2012 v.96 no.4 pp. 1071-1078
ISSN:
0175-7598
Subject:
Actinomycetales; Escherichia coli O157; Staphylococcus aureus; Staphylococcus epidermidis; antibiotic resistance; antimicrobial agents; bacteria; biofilm; culture media; electron microscopy; indole acetic acid; operon; plant pathogens; screening; transcriptomics; tryptophan 2,3-dioxygenase
Abstract:
... Pathogenic biofilms have been associated with persistent infections due to their high resistance to antimicrobial agents. To identify nontoxic biofilm inhibitors for enterohemorrhagic Escherichia coli O157:H7, the spent media of a 4,104 Actinomycetes library was screened. The culture spent medium (1%, v/v) of plant pathogen Rhodococcus sp. BFI 332 markedly inhibited E. coli O157:H7 biofilm formati ...
DOI:
10.1007/s00253-012-3881-y
PubMed:
22274708

48. Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation

Author:
Lee, Jintae,   et al.   ; Park, Joo-Hyeon; Lee, Jin-Hyung; Kim, Chang-Jin; Lee, Jae-Chan; Cho, Moo Hwan;     Show all 6 Authors
Source:
Biotechnology letters 2012 v.34 no.4 pp. 655-661
ISSN:
0141-5492
Subject:
Actinomycetales; Staphylococcus aureus; antibiotic resistance; antimicrobial agents; biofilm; medical equipment; pathogens; proteinases
Abstract:
... Bacterial biofilms are associated with chronic infections due to their resistance to antimicrobial agents. Staphylococcus aureus is a versatile human pathogen and can form biofilms on human tissues and diverse medical devices. To identify novel biofilm inhibitors of S. aureus, the supernatants from a library of 458 Actinomycetes strains were screened. The culture supernatants (1% v/v) of more than ...
DOI:
10.1007/s10529-011-0825-z
PubMed:
22160331

49. Temporal gene-expression in Escherichia coli K-12 biofilms

Author:
Lee, Jintae,   et al.   ; Domka, Joanna; Bansal, Tarun; Wood, Thomas K.;     Show all 4 Authors
Source:
Environmental microbiology 2007 v.9 no.2 pp. 332-346
ISSN:
1462-2912
Subject:
Escherichia coli; biofilm; biosynthesis; cell suspension culture; extracellular matrix; fimbriae; galactitol; galactosamine; gene deletion; gene expression; genes; mutants; phosphates; proteins; quorum sensing; stress response; sulfates; sulfides; tryptophan
Abstract:
... Analysis of the temporal development of Escherichia coli K-12 biofilms in complex medium indicates the greatest differential gene expression between biofilm and suspension cells occurred in young biofilms at 4 and 7 h (versus 15 and 24 h). The main classes of genes differentially expressed (biofilm versus biofilm and biofilm versus suspension cells) include 42 related to stress response (e.g. cspA ...
DOI:
10.1111/j.1462-2920.2006.01143.x
PubMed:
17222132

50. Enterohemorrhagic Escherichia coli Biofilms Are Inhibited by 7-Hydroxyindole and Stimulated by Isatin

Author:
Lee, Jintae,   et al.   ; Bansal, Tarun; Jayaraman, Arul; Bentley, William E.; Wood, Thomas K.;     Show all 5 Authors
Source:
Applied and environmental microbiology 2007 v.73 no.13 pp. 4100-4109
ISSN:
0099-2240
Subject:
Escherichia coli O157; Pseudomonas aeruginosa; biofilm; cell growth; cysteine; genes; metabolism; microscopy; mutants; polystyrenes; transporters; virulence
Abstract:
... Since indole is present at up to 500 μM in the stationary phase and is an interspecies biofilm signal (J. Lee, A. Jayaraman, and T. K. Wood, BMC Microbiol. 7:42, 2007), we investigated hydroxyindoles as biofilm signals and found them also to be nontoxic interspecies biofilm signals for enterohemorrhagic Escherichia coli O157:H7 (EHEC), E. coli K-12, and Pseudomonas aeruginosa. The genetic basis of ...
DOI:
10.1128/AEM.00360-07
PubMed:
17483266
PubMed Central:
PMC1932762

51. Differential Effects of Epinephrine, Norepinephrine, and Indole on Escherichia coli O157:H7 Chemotaxis, Colonization, and Gene Expression

Author:
Lee, Jintae,   et al.   ; Bansal, Tarun; Englert, Derek; Hegde, Manjunath; Wood, Thomas K.; Jayaraman, Arul;     Show all 6 Authors
Source:
Infection and immunity 2007 v.75 no.9 pp. 4597-4607
ISSN:
0019-9567
Subject:
Escherichia coli O157; biofilm; chemotaxis; epinephrine; epithelial cells; gene expression; gene expression regulation; genes; large intestine; microarray technology; norepinephrine; phenotype; virulence
Abstract:
... During infection in the gastrointestinal tract, enterohemorrhagic Escherichia coli (EHEC) O157:H7 is exposed to a wide range of signaling molecules, including the eukaryotic hormones epinephrine and norepinephrine, and bacterial signal molecules such as indole. Since these signaling molecules have been shown to be involved in the regulation of phenotypes such as motility and virulence that are cru ...
DOI:
10.1128/IAI.00630-07
PubMed:
17591798
PubMed Central:
PMC1951185

52. Motility influences biofilm architecture in Escherichia coli

Author:
Lee, Jintae,   et al.   ; Wood, Thomas K.; González Barrios, Andrés F.; Herzberg, Moshe;     Show all 4 Authors
Source:
Applied microbiology and biotechnology 2006 v.72 no.2 pp. 361-367
ISSN:
0175-7598
Subject:
Escherichia coli; biofilm; flagellum; loci; microscopy; mutants
Abstract:
... Eight Escherichia coli strains were studied in minimal medium with a continuous flow system using confocal microscopy. K12 wild-type strains ATCC 25404 and MG1655 formed the best biofilms (~43 μm thick, 21 to 34% surface coverage). JM109, DH5α, and MG1655 motA formed intermediate biofilms (~13 μm thick, 41 to 58% surface coverage). BW25113, MG1655 qseB, and MG1655 fliA had poor biofilms (surface c ...
DOI:
10.1007/s00253-005-0263-8
PubMed:
16397770