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101. Lutibacter agarilyticus sp. nov., a marine bacterium isolated from shallow coastal seawater

Author:
Choe, Han Na,   et al.   ; Park, Seong Chan; Hwang, Yeoung Min; Baik, Keun Sik; Seong, Chi Nam;     Show all 5 Authors
Source:
International journal of systematic and evolutionary microbiology 2013 v.63 no.Pt 7 pp. 2678-2683
ISSN:
1466-5034
Subject:
DNA; bacteria; coastal water; fatty acids; nucleotide sequences; phylogeny; ribosomal RNA; seawater; sequence homology; Korean Peninsula
Abstract:
... A Gram-staining-negative, non-spore-forming, non-gliding and rod-shaped bacterial strain KYW566T, was isolated from seawater of the Suncheon Bay, Korea, and its taxonomic position was investigated by using a polyphasic study. The cells contained MK-6 as the only respiratory quinone and contained iso-C15 : 0 (13.8 %), iso-C16 : 0 3-OH (13.1 %), anteiso-C15 : 0 (9.3 %), iso-C15 : 0 3-OH (8.7 %), iso ...
DOI:
10.1099/ijs.0.047670-0

102. Extraction optimization of polysaccharides of Schisandrae Fructus and evaluation of their analgesic activity

Author:
Han, Na,   et al.   ; Ye, Chun; Teng, Fukang; Wang, Xiaokang; Xue, Rui; Yin, Jun;     Show all 6 Authors
Source:
International journal of biological macromolecules 2013 v.57 pp. 291-296
ISSN:
0141-8130
Subject:
acetic acid; analgesic effect; analgesics; high performance liquid chromatography; intraperitoneal injection; latent period; mice; polysaccharides; raw materials
Abstract:
... Polysaccharide from the Schisandrae Fructus (SFP) has been considered as the major effective component with many activities and high content. To obtain SFP more efficiently and clear up its analgesic activity, the three-factor, three-level orthogonal extracting test was designed to optimize the extraction condition based on the results of single-factor experiments. The optimal parameters were dete ...
DOI:
10.1016/j.ijbiomac.2013.03.025

103. Aquimarina gracilis sp. nov., isolated from the gut microflora of a mussel, Mytilus coruscus, and emended description of Aquimarina spongiae

Author:
Choe, Han Na,   et al.   ; Park, Seong Chan; Baik, Keun Sik; Seong, Chi Nam;     Show all 4 Authors
Source:
International journal of systematic and evolutionary microbiology 2013 v.63 no.Pt 5 pp. 1782-1787
ISSN:
1466-5034
Subject:
DNA; Mytilus; bacteria; fatty acids; intestinal microorganisms; mussels; nucleotide sequences; phosphatidylethanolamines; phylogeny; ribosomal RNA; South Korea
Abstract:
... An orange-coloured and slender rod-shaped bacterium, designated strain PSC32T, was isolated from the gut microflora of a mussel collected from Gwangyang Bay, South Sea (Republic of Korea). Cells were Gram-reaction-negative, strictly aerobic, and catalase- and oxidase-positive. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 3-OH. The only isoprenoid quinone ...
DOI:
10.1099/ijs.0.044289-0

104. Cardenolide glycosides from root of Streptocaulon juventas

Author:
Han, Na,   et al.   ; Xue, Rui; Ye, Chun; Wang, Hai-bo; Yin, Jun;     Show all 5 Authors
Source:
Phytochemistry 2013 v.88 pp. 105-111
ISSN:
0031-9422
Subject:
Apocynaceae; adenocarcinoma; cytotoxicity; digitoxigenin; glycosides; humans; roots; spectroscopy; China
Abstract:
... Six cardenolides were isolated from the anti-tumor active fraction of the 75% ethanol extract of Streptocaulon juventas (Asclepiadaceae), mainly found in southwest of China. These were named 1α, 14β-dihydroxy-5β-card-20 (22)-enolide 3-O-[O-β-d-glucopyranosyl-(1→2)-β-d-digitalopyranoside] (1), acovenosigenin A 3-O-[O-β-d-glucopyranosyl-(1→4)-β-d-digitalopyranoside] (2), 16-O-acetyl-hydroxyperiploge ...
DOI:
10.1016/j.phytochem.2012.12.004
PubMed:
23286880

105. Molecular characterization of China human rabies vaccine strains

Author:
Han, Na,   et al.   ; Tao, Xiaoyan; Guo, Zhenyang; Tang, Qing; Rayner, Simon; Liang, Guodong;     Show all 6 Authors
Source:
Virologica Sinica 2013 v.28 no.2 pp. 116-123
ISSN:
1674-0769
Subject:
Vesiculovirus; crystal structure; genome; humans; mutation; rabies; topology; virulence; viruses
Abstract:
... To understand the molecular characteristics of China human rabies vaccine strains, we report the full-length genome of the aG strain and present a comprehensive analysis of this strain and almost all available lyssavirus genomes (58 strains) from GenBank (as of Jan 6, 2011). It is generally considered that the G protein plays a predominant role in determining the pathogenicity of the virus, to thi ...
DOI:
10.1007/s12250-013-3314-9
PubMed:
23575734

106. Multilineage differentiation of human-derived dermal fibroblasts transfected with genes coated on PLGA nanoparticles plus growth factors

Author:
Yang, Han Na,   et al.   ; Park, Ji Sun; Woo, Dae Gyun; Jeon, Su Yeon; Park, Keun-Hong;     Show all 5 Authors
Source:
Biomaterials 2013 v.34 pp. 582-597
ISSN:
0142-9612
Subject:
Western blotting; adipogenesis; bone formation; chondrogenesis; confocal laser scanning microscopy; fibrin; fibroblasts; gels; genes; mice; nanoparticles; polymers; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; somatomedins; tissue repair; transforming growth factor beta 3
Abstract:
... Wounded tissues and cells may be treated with growth factors and specific genes for the purpose of tissue repair and regeneration. To deliver specific genes into tissues and cells, this study presents the use of fabricated poly (dl-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) complexed with the cationic polymer poly (ethleneimine) (PEI). Through complexation with PEI, several types of genes ...
DOI:
10.1016/j.biomaterials.2012.10.001

107. Synthesis and characterization of novel sulfur-containing 2-(1H-pyrrolyl) carboxylic acids and their effects on garlic greening

Author:
Han, Na,   et al.   ; Yang, Haixia; Zhang, Jian; Bai, Yufei; Zhao, Guanghua;     Show all 5 Authors
Source:
European food research & technology 2010 v.231 no.4 pp. 555-561
ISSN:
1438-2377
Subject:
bulbs; carboxylic acids; color; garlic; green leafy vegetables; nonprotein amino acids; pigments; proteins; structure-activity relationships
Abstract:
... It has been established that protein amino acids are involved in garlic greening by reacting with thiosulfinates to form pigment precursors (PP), a class of 2-(1H-pyrrolyl) carboxylic acids. This opens an interesting question of whether specific sulfur-containing non-protein amino acids naturally occurring in garlic such as derivates of cysteine and their corresponding sulfoxides also play a role ...
DOI:
10.1007/s00217-010-1306-7

108. Transfection of VEGF₁₆₅ genes into endothelial progenitor cells and in vivo imaging using quantum dots in an ischemia hind limb model

Author:
Yang, Han Na,   et al.   ; Park, Ji Sun; Woo, Dae Gyun; Jeon, Su Yeon; Park, Keun-Hong;     Show all 5 Authors
Source:
Biomaterials 2012 v.33 no.33 pp. 8670-8684
ISSN:
0142-9612
Subject:
animal models; atomic force microscopy; blood; endothelial cells; genes; image analysis; ischemia; light scattering; mice; nanoparticles; plasmids; protein synthesis; proteins; quantum dots; scanning electron microscopy; stem cells; transfection
Abstract:
... Endothelial progenitor cells (EPCs) were transfected with fluorescently labeled quantum dot nanoparticles (QD NPs) with or without VEGF₁₆₅ plasmid DNA (pDNA) to probe the EPCs after in vivo transplantation and to test whether they presented as differentiated endothelial cells (ECs). Bare QD NPs and QD NPs coated with PEI or PEI + VEGF₁₆₅ genes were characterized by dynamic light scattering, scanni ...
DOI:
10.1016/j.biomaterials.2012.08.012

109. Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells

Author:
Lee, Han-Na,   et al.   ; Park, Soo-Hyun; Lee, Eun-Kyung; Bernardo, Raymundo; Kim, Chan-Wha;     Show all 5 Authors
Source:
Biochemical and biophysical research communications 2012 v.427 no.1 pp. 171-177
ISSN:
0006-291X
Subject:
colorectal neoplasms; humans; metastasis; neoplasm cells; proteins; proteomics; stem cells
Abstract:
... Recent reports have suggested that tumors are organized in heterogeneous populations. Within these populations, a small subpopulation of cells is more capable of initiating malignancy; these are called cancer stem cells. In this study, HT-29 cells were sorted according to the presence or absence of the cancer stem cell marker CD133. We confirmed that CD133+ cells possessed higher clonogenicity com ...
DOI:
10.1016/j.bbrc.2012.09.036

110. SOX9 gene plus heparinized TGF-β 3 coated dexamethasone loaded PLGA microspheres for inducement of chondrogenesis of hMSCs

Author:
Yang, Han Na,   et al.   ; Park, Ji Sun; Woo, Dae Gyun; Jeon, Su Yeon; Park, Keun-Hong;     Show all 5 Authors
Source:
Biomaterials 2012 v.33 no.29 pp. 7151-7163
ISSN:
0142-9612
Subject:
adhesion; chondrogenesis; coatings; collagen; dexamethasone; extracellular matrix; genes; in vitro culture; stem cells; therapeutics; tissue engineering; tissues; transforming growth factor beta 3
Abstract:
... Microparticulated types of scaffolds have been widely applied in stem cell therapy and the tissue engineering field for the regeneration of wound tissues. During application of simple genes or growth factors and cell delivery vehicles, we designed a method that employs dexamethsone loaded PLGA microspheres consisting of polyplexed SOX9 genes plus heparinized TGF-β 3 on the surface of polymeric mic ...
DOI:
10.1016/j.biomaterials.2012.06.023

111. Aquimarina mytili sp. nov., isolated from the gut microflora of a mussel, Mytilus coruscus, and emended description of Aquimarina macrocephali

Author:
Choe, Han Na,   et al.   ; Park, Seong Chan; Baik, Keun Sik; Seong, Chi Nam;     Show all 4 Authors
Source:
International journal of systematic and evolutionary microbiology 2012 v.62 no.Pt 8 pp. 1974-1979
ISSN:
1466-5034
Subject:
DNA; Mytilus; bacteria; fatty acids; intestinal microorganisms; mussels; nucleotide sequences; phylogeny; ribosomal RNA; sequence homology; South Korea
Abstract:
... An orange, rod-shaped, gliding bacterium, designated strain PSC33T, was isolated from the gut microflora of a mussel collected from Gwangyang Bay, South Sea (Republic of Korea). Cells were Gram-reaction-negative, strictly aerobic, and catalase- and oxidase-positive. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G, C15 : 0 3-OH and iso-C17 : 1ω9c. The only isoprenoid quinone ...
DOI:
10.1099/ijs.0.032904-0

112. Co-delivery of SOX9 genes and anti-Cbfa-1 siRNA coated onto PLGA nanoparticles for chondrogenesis of human MSCs

Author:
Yang, Han Na,   et al.   ; Jeon, Su Yeon; Park, Ji Sun; Woo, Dae Gyun; Park, Keun-Hong;     Show all 5 Authors
Source:
Biomaterials 2012 v.33 no.17 pp. 4413-4423
ISSN:
0142-9612
Subject:
Western blotting; cell differentiation; chondrogenesis; ethyleneimine; fluorescent antibody technique; gels; gene expression; genes; green fluorescent protein; humans; image analysis; immunohistochemistry; light scattering; nanoparticles; plasmids; polymers; reverse transcriptase polymerase chain reaction; scanning electron microscopes; scanning electron microscopy; small interfering RNA; stem cells; transfection
Abstract:
... Some genes expressed in stem cells interrupt and/or enhance differentiation. Therefore, the aim of this study was to inhibit the expression of unnecessary genes and enhance the expression of specific genes involved in stem cell differentiation by using small interfering RNA (siRNA) and plasmid DNA (pDNA) incorporated into cationic polymers as co-delivery factors. To achieve co-delivery of siRNA an ...
DOI:
10.1016/j.biomaterials.2012.02.051

113. Antithrombotic activity of fractions and components obtained from raspberry leaves (Rubus chingii)

Author:
Han, Na,   et al.   ; Gu, Yuhong; Ye, Chun; Cao, Yan; Liu, Zhihui; Yin, Jun;     Show all 6 Authors
Source:
Food chemistry 2012 v.132 no.1 pp. 181-185
ISSN:
0308-8146
Subject:
Rubus; ethanol; kaempferol; leaves; quercetin; raspberries; salicylic acid
Abstract:
... The 70% ethanol fraction from an aqueous extract of raspberry leaves was shown to be the most antithrombotic fraction in in vitro and in vivo tests. The total flavonoids and phenolics in this fraction were 0.286g/g and 0.518g/g by colorimetry. Six compounds, including salicylic acid, kaempferol, quercetin, tiliroside, quercetin 3-O-β-d-glucopyranoside and kaempferol 3-O-β-d-glucopyranoside, were ...
DOI:
10.1016/j.foodchem.2011.10.051

114. Minimal polyketide pathway expression in an actinorhodin cluster-deleted and regulation-stimulated Streptomyces coelicolor

Author:
Lee, Han-Na,   et al.   ; Kim, Hye-Jin; Kim, Pil; Lee, Heung-Shick; Kim, Eung-Soo;     Show all 5 Authors
Source:
Journal of industrial microbiology & biotechnology 2012 v.39 no.5 pp. 805-811
ISSN:
1367-5435
Subject:
Streptomyces coelicolor; antibiotics; biosynthesis; chromosomes; multigene family; mutants; plasmids; regulator genes; secondary metabolites
Abstract:
... Along with traditional random mutagenesis-driven strain improvement, cloning and heterologous expression of Streptomyces secondary metabolite gene clusters have become an attractive complementary approach to increase its production titer, of which regulation is typically under tight control via complex multiple regulatory networks present in a metabolite low-producing wild-type strain. In this stu ...
DOI:
10.1007/s10295-011-1083-8
PubMed:
22252445

115. Methallyl isothiocyanate inhibits the caspase-1 activity through the inhibition of intracellular calcium levels

Author:
Han, Na-Ra,   et al.   ; Kim, Il-Kwang; Kim, Hyung-Min; Jeong, Hyun-Ja;     Show all 4 Authors
Source:
Biochimie 2012 v.94 no.3 pp. 816-822
ISSN:
0300-9084
Subject:
IKappaB kinase; acetates; anti-inflammatory activity; calcium; caspase-1; chemoprevention; enzyme activity; gene expression; humans; interleukin-6; isothiocyanates; mast cells; messenger RNA; mitogen-activated protein kinase; phosphorylation; reactive oxygen species; transcription factor NF-kappa B; tumor necrosis factor-alpha; vegetables
Abstract:
... Isothiocyanates (ITCs) play a major role in the potential chemopreventive effect. Cruciferous vegetables are a particularly abundant source of ITCs. Methallyl ITC (MAITC) belongs to ITCs as a synthesized compound. However, the effects of MATIC have never been elucidated. The aim of this work was to investigate the anti-inflammatory effects and mechanisms of methallyl isothiocyanate (MAITC) in mast ...
DOI:
10.1016/j.biochi.2011.11.013

116. Effect of Pyeongwee-San (KMP6) on 2,4-dinitrofluorobenzene-induced atopic dermatitis-like skin lesions in NC/Nga mice

Author:
Han, Na-Ra,   et al.   ; Moon, Phil-Dong; Kim, Hyung-Min; Jeong, Hyun-Ja;     Show all 4 Authors
Source:
Life sciences 2012 v.90 no.3-4 pp. 147-153
ISSN:
0024-3205
Subject:
atopic dermatitis; blood serum; digestive tract; gene expression; gene expression regulation; glycyrrhizin; histamine; immunoglobulin E; interferon-gamma; interleukin-4; mast cells; medicine; messenger RNA; mice; models; skin lesions; splenocytes; stomach; tumor necrosis factor-alpha
Abstract:
... AIMS: Recently, some studies reported that digestive tract disease is closely associated with atopic dermatitis (AD). Pyeongwee-San (KMP6) is a Korean medicine, which has come onto the drugstore for the treatment of digestive tract disease. The aim of the present study was to examine whether KMP6 could suppress 2,4-dinitrofluorobenzene (DNFB)-induced AD-like skin lesions in NC/Nga mice. MAIN METHO ...
DOI:
10.1016/j.lfs.2011.10.015

117. Porcine placenta mitigates protein–energy malnutrition-induced fatigue

Author:
Han, Na-Ra,   et al.   ; Kim, Kyu-Yeop; Kim, Myong-Jo; Kim, Min-Ho; Kim, Hyung-Min; Jeong, Hyun-Ja;     Show all 6 Authors
Source:
Nutrition 2013 v.29 no.11-12 pp. 1381-1387
ISSN:
0899-9007
Subject:
T-lymphocytes; arginine; calcium; cytokines; cytosol; diet; glutamic acid; immunity; in vitro studies; malnutrition; messenger RNA; mice; muscles; nutrient deficiencies; placenta; proline; protein synthesis; splenocytes; swine
Abstract:
... OBJECTIVE: Fatigue can be caused by a deficiency of nutrition or immune function. The goal of this study was to identify the effects of porcine placenta extract (PPE) and its constituents, amino acids (glutamic acid, glycine, arginine, and proline), on protein–energy malnutrition (PEM)-induced fatigue. METHODS: Mice were administered a PEM diet and came to immunodeficient status. Simultaneously, t ...
DOI:
10.1016/j.nut.2013.04.016
PubMed:
24035056

118. Algibacter aquimarinus sp. nov., isolated from a marine environment, and reclassification of Pontirhabdus pectinivorans as Algibacter pectinivorans comb. nov.

Author:
Choe, Han Na,   et al.   ; Park, Seong Chan; Hwang, Yeoung Min; Baik, Keun Sik; Kim, Hyangmi; Seong, Chi Nam;     Show all 6 Authors
Source:
International journal of systematic and evolutionary microbiology 2013 v.63 no.Pt 6 pp. 2038-2042
ISSN:
1466-5034
Subject:
DNA; Flavobacteriaceae; fatty acids; marine environment; microbiology; nucleotide sequences; phosphatidylethanolamines; phylogeny; pigments; ribosomal RNA; salts; seawater; taxonomic revisions; South Korea
Abstract:
... An orange-coloured, rod-shaped, Gram-reaction-negative and aerobic bacterial strain, designated KYW589T, was isolated from seawater collected from Gwangyang Bay, Republic of Korea. The isolate required sea salts for growth. Gliding motility was observed. Flexirubin-type pigments were absent. Major cellular fatty acids (>10 % of the total) were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G, C16 : 0, ...
DOI:
10.1099/ijs.0.045815-0

119. Sphingopyxis rigui sp. nov. and Sphingopyxis wooponensis sp. nov., isolated from wetland freshwater, and emended description of the genus Sphingopyxis

Author:
Choe, Han Na,   et al.   ; Baik, Keun Sik; Park, Seong Chan; Hwang, Yeoung Min; Kim, Eun Mi; Park, Chul; Seong, Chi Nam;     Show all 7 Authors
Source:
International journal of systematic and evolutionary microbiology 2013 v.63 no.Pt 4 pp. 1297-1303
ISSN:
1466-5034
Subject:
Sphingomonas; aerobes; freshwater; microbiology; nucleic acid hybridization; nucleotide sequences; phylogeny; polymerase chain reaction; ribosomal RNA; sequence homology; wetlands; South Korea
Abstract:
... Two yellow-pigmented, Gram-reaction-negative strains, designated 01SU5-PT and 03SU3-PT, were isolated from the freshwater of Woopo wetland, Republic of Korea. Both strains were aerobic, non-motile and catalase-negative. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates belong to the genus Sphingopyxis, showing the highest level of sequence similarity with respe ...
DOI:
10.1099/ijs.0.044057-0

120. Ambiguanine A–G, hexahydrobenzophenanthridine alkaloids from Corydalis ambigua var. amurensis

Author:
Han, Na,   et al.   ; Yang, Zhi-You; Jiang, Bao-Hong; Teng, Fu-Kang; Liu, Zhi-Hui; Zhou, Jing-Jing; Wang, Yu; Guo, De-An; Yin, Jun;     Show all 9 Authors
Source:
Phytochemistry 2014 v.105 pp. 158-163
ISSN:
0031-9422
Subject:
Corydalis; alkaloids; spectroscopy; tubers
Abstract:
... Seven hexahydrobenzophenanthridine-type alkaloids, Ambiguanine A–G, along with eight known alkaloids, were isolated from tubers of Corydalis ambigua var. amurensis. Their structures were elucidated based on extensive spectroscopic analyses, with absolute configurations determined by CD experiments. ...
DOI:
10.1016/j.phytochem.2014.05.013