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121. Potential Anticoagulant Activity of Trypsin Inhibitor Purified from an Isolated Marine Bacterium Oceanimonas Sp. BPMS22 and its Kinetics

Author:
Harish, B. S.; Uppuluri, Kiran Babu
Source:
Marine biotechnology 2018 v.20 no.6 pp. 780-791
ISSN:
1436-2228
Subject:
trypsin inhibitors,   etc   ; Oceanimonas; affinity chromatography; anion exchange; anticoagulant activity; aquatic bacteria; bacterial growth; blood sampling; enzyme activity; homeostasis; humans; marine sediments; molecular weight; pH; salt concentration; salt tolerance; temperature; trypsin;     Show all 18 Subjects
Abstract:
... Protease inhibitors control major biological protease activities to maintain physiological homeostasis. Marine bacteria isolated from oligotrophic conditions could be taxonomically distinct, metabolically unique, and offers a wide variety of biochemicals. In the present investigation, marine sediments were screened for the potential bacteria that can produce trypsin inhibitors. A moderate halotole ...
DOI:
10.1007/s10126-018-9848-y

122. Potential for enhanced soy storage protein breakdown and allergen reduction in soy-based foods produced with optimized sprouted soybeans

Author:
Oyedeji, Ajibola B.; Mellem, John J.; Ijabadeniyi, Oluwatosin A.
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.98 pp. 540-545
ISSN:
0023-6438
Subject:
trypsin inhibitors,   etc   ; Hibiscus sabdariffa; allergens; beta-conglycinin; calcium chloride; flower extracts; glycinin; image analysis; matrix-assisted laser desorption-ionization mass spectrometry; proteomics; soybeans; soymilk; tofu; two-dimensional gel electrophoresis;     Show all 14 Subjects
Abstract:
... In this study, potentials for improved soy storage protein breakdown and allergen reduction in soy-based foods produced with soybeans sprouted at optimized conditions was investigated using proteomic approach. The changes in protein concentration and expression levels of six sprouted soy-based foods, namely SSM: sprouted soymilk, STC: tofu from sprouted soybeans coagulated by CaCl2, STH: tofu from ...
DOI:
10.1016/j.lwt.2018.09.019

123. Quantitative proteomic analyses reveal that RBBI3.3, a trypsin inhibitor protein, plays an important role in Magnaporthe oryzae infection in rice

Author:
Cui, Shujian; Wang, Jiaoyu; Gao, Feiyan; Sun, Guochang; Liang, Jiansheng
Source:
Plant growth regulation 2018 v.86 no.3 pp. 365-374
ISSN:
0167-6903
Subject:
trypsin inhibitors,   etc   ; Magnaporthe oryzae; appressoria; blast disease; breeding; cultivars; disease control; disease resistance; fungal diseases of plants; genes; mass spectrometry; models; phenotype; plant pathogenic fungi; proteins; proteomics; rice; China;     Show all 18 Subjects
Abstract:
... Magnaporthe oryzae (M. oryzae) is the causative agent of rice blast, the most destructive rice disease in China. This study was designed to ascertain the molecular mechanisms of the response of rice to M. oryzae infection to facilitate the breeding of new high-quality and disease-resistant rice varieties using isobaric tags for relative and absolute quantification (iTRAQ) combined with a high-thro ...
DOI:
10.1007/s10725-018-0435-z

124. Viscera of Labeo rohita: A Potential Source of Trypsin for Industrial Application

Author:
Khangembam, Bronson Kumar; Chakrabarti, Rina
Source:
Journal of aquatic food product technology 2018 v.27 no.10 pp. 1078-1092
ISSN:
1547-0636
Subject:
trypsin inhibitors,   etc   ; Labeo rohita; affinity chromatography; aluminum; ammonium sulfate; animal organs; cadmium; calcium chloride; carp; catalytic activity; cobalt; enzyme activity; industrial applications; ion exchange; ions; lithium; magnesium; mercury; molecular weight; pH; potassium; soybeans; temperature; trypsin; zinc;     Show all 25 Subjects
Abstract:
... The serine protease trypsin was isolated and purified from the digestive system of carp Labeo rohita rohu by ammonium sulphate precipitation, ion exchange, and affinity chromatography. The purified enzyme showed high activity between pH 7.0 and 9.0. The activity was maximum at 40°C. Incubation of the purified enzyme with CaCl₂ (2 mM) stabilized the enzyme activity for 8 h. The enzyme showed stabil ...
DOI:
10.1080/10498850.2018.1534300

125. Wheat ATI CM3, CM16 and 0.28 Allergens Produced in <i>Pichia Pastoris</i> Display a Different Eliciting Potential in Food Allergy to Wheat <sup>‡</sup>

Author:
Tundo, Silvio; Lupi, Roberta; Lafond, Mickael; Giardina, Thierry; Larré, Colette; Denery-Papini, Sandra; Morisset, Martine; Kalunke, Raviraj; Sestili, Francesco; Masci, Stefania
Source:
Plants 2018 v.7 no.4
ISSN:
2223-7747
Subject:
trypsin inhibitors,   etc   ; Komagataella pastoris; Triticum; allergenicity; allergens; alpha-amylase; basophils; beta-N-acetylhexosaminidase; celiac disease; food allergies; genes; human population; humans; innate immunity; messenger RNA; patients; polypeptides; recombinant fusion proteins; seeds; staple foods; wheat;     Show all 21 Subjects
Abstract:
... Although wheat is a staple food for most of the human population, some of its components trigger adverse reactions. Among wheat components, the alpha-amylase/trypsin inhibitors (ATI) are important triggers of several allergies and activators of innate immunity. ATI are a group of exogenous protease inhibitors and include several polypeptides. The three ATI polypeptides named CM3, CM16 and 0.28 are ...
DOI:
10.3390/plants7040101

126. Lupinus angustifolius L. lactofermentation and protein isolation: effects on phenolic compounds and genistein, antioxidant properties, trypsin inhibitor activity, and protein digestibility

Author:
Bartkiene, Elena; Sakiene, Vytaute; Bartkevics, Vadims; Rusko, Janis; Lele, Vita; Juodeikiene, Grazina; Wiacek, Claudia; Braun, Peggy G.
Source:
European food research & technology 2018 v.244 no.9 pp. 1521-1531
ISSN:
1438-2377
Subject:
trypsin inhibitors,   etc   ; Lupinus angustifolius; Pediococcus pentosaceus; antioxidant activity; digestible protein; food research; genistein; hybrids; molecular weight; phenolic compounds; polyacrylamide gel electrophoresis; protein content; protein isolates; seeds; solids; submerged fermentation; Europe;     Show all 17 Subjects
Abstract:
... In some European countries, lupin seeds are not used efficiently enough, due to a lack of processing technologies for the preparation of attractive higher-value products; fermentation could be a promising process to increase the value of lupin seeds and their protein isolates. In this study, the influence of a range of factors—seeds of Lupinus angustifolius L. hybrid line (HL) Nos. 1700, 1701, 180 ...
DOI:
10.1007/s00217-018-3066-8

127. Transcriptomic and proteomic analyses of drought responsive genes and proteins in Agropyron mongolicum Keng

Author:
Zhao, Yan; Gao, Cuiping; Shi, Fengling; Yun, Lan; Jia, Yushan; Wen, Jiangqi
Source:
Current plant biology 2018 v.14 pp. 19-29
ISSN:
2214-6628
Subject:
trypsin inhibitors,   etc   ; Agropyron mongolicum; amylases; antioxidants; biosynthesis; cell walls; chlorophyll; chloroplasts; databases; dehydrins; drought; drought tolerance; flavonoids; forage grasses; galactose; gene expression regulation; genomics; hydrolysis; pentose phosphate cycle; proline; proteomics; sequence homology; storage proteins; structural proteins; transcriptomics; unigenes; water stress;     Show all 27 Subjects
Abstract:
... Agropyron mongolicum Keng is a drought-tolerant perennial forage grass. To elucidate the underlying mechanisms in response to drought stress, we performed transcriptomic and proteomic profiling analyses under mild and severe drought conditions. After de novo assembly, 92,752 unigenes were obtained with an average size of 791 bp and N50 of 1303 bp. 70,732 (76.26%) unigenes showed high sequence simi ...
DOI:
10.1016/j.cpb.2018.09.005

128. Detoxification of Jatropha curcas seed cake by a new soil‐borne Enterobacter cloacae strain

Author:
Zhao, Y.N.; Wang, H.F.; Liu, J.X.
Source:
Letters in applied microbiology 2018 v.67 no.2 pp. 197-204
ISSN:
0266-8254
Subject:
trypsin inhibitors,   etc   ; Enterobacter cloacae; Jatropha curcas; antinutritional factors; byproducts; esters; inoculum; lectins; methodology; microbial growth; nutritive value; oils; oilseed cakes; pH; protein content; seeds; soil; solid state fermentation; tannins; temperature; toxicity; toxins; water content;     Show all 23 Subjects
Abstract:
... Jatropha curcas seed cake is a by‐product generated after oil extraction from J. curcas seeds. Although the protein content is high, the cake contains phorbol esters and antinutritional factors such as phytates, trypsin inhibitors, lectins and tannins. Therefore, it cannot be directly used in food or feed. In this study, the toxic compounds and antinutrients present in J. curcas seed cake were det ...
DOI:
10.1111/lam.13012

129. Effects of detoxified Jatropha curcas protein isolate on growth performance, nutrient digestibility and physio‐metabolic response of Labeo rohita fingerlings

Author:
Fawole, F.J.; Sahu, N.P.; Shamna, N.; Phulia, V.; Emikpe, B.O.; Adeoye, A.A.; Aderolu, A.Z.; Popoola, O.M.
Source:
Aquaculture nutrition 2018 v.24 no.4 pp. 1223-1233
ISSN:
1353-5773
Subject:
trypsin inhibitors,   etc   ; Jatropha curcas; Labeo rohita; alanine transaminase; alkaline phosphatase; antinutritional factors; blood serum; chemical precipitation; digestibility; esters; feed conversion; fingerlings; growth performance; intestines; lipids; lysine; phytic acid; protein efficiency ratio; protein isolates; soy protein; specific growth rate; weight gain;     Show all 22 Subjects
Abstract:
... Jatropha protein isolate was prepared following the principle of isoelectric precipitation. The isolated protein is low in fibre, lipid and ash, and high in protein and gross energy contents. The antinutritional factors such as phytic acid, tannin and trypsin inhibitor were found to decrease after protein extraction except phorbol esters (PEs), which remain bounded to the protein matrix. Thus, the ...
DOI:
10.1111/anu.12660

130. Nutraceuticals in gummy candies form prepared from lacto‐fermented lupine protein concentrates, as high‐quality protein source, incorporated with Citrus paradise L. essential oil and xylitol

Author:
Bartkiene, Elena; Sakiene, Vytaute; Bartkevics, Vadims; Wiacek, Claudia; Rusko, Janis; Lele, Vita; Ruzauskas, Modestas; Juodeikiene, Grazina; Klupsaite, Dovile; Bernatoniene, Jurga; Jakstas, Valdas; Braun, Peggy G.
Source:
International journal of food science & technology 2018 v.53 no.8 pp. 2015-2025
ISSN:
0950-5423
Subject:
trypsin inhibitors,   etc   ; Citrus paradisi; Lactobacillus sakei; Lupinus; active ingredients; ascorbic acid; digestible protein; essential oils; fermentation; functional foods; genistein; glycemic index; grapefruits; protein concentrates; protein content; protein sources; taste; texture; xylitol;     Show all 19 Subjects
Abstract:
... As an addition to a normal diet, the use of food enriched with functional compounds has become increasingly popular. Alternatively, functional ingredients can be incorporated into nutraceuticals in gummy candies (GC) form. This study aimed to adapt lacto‐fermented lupine protein concentrates, as high‐quality protein and genistein sources, with Citrus paradise essential oil (EO), an antimicrobial a ...
DOI:
10.1111/ijfs.13819

131. Molecular docking and in vitro studies of soap nut trypsin inhibitor (SNTI) against phospholipase A2 isoforms in therapeutic intervention of inflammatory diseases

Author:
Sirisha, Gandreddi V.D.; Vijaya Rachel, K.; Zaveri, Kunal; Yarla, Nagendra Sastry; Kiranmayi, P.; Ganash, Magdah; Alkreathy, Huda Mohammad; Rajeh, Nisreen; Ashraf, Ghulam Md
Source:
International journal of biological macromolecules 2018 v.114 pp. 556-564
ISSN:
0141-8130
Subject:
trypsin inhibitors,   etc   ; Sapindus trifoliatus; adverse effects; allelochemicals; anti-inflammatory agents; cytosol; endosperm; immunomodulators; in vitro studies; inflammation; inhibitory concentration 50; ligands; molecular models; pancreas; phospholipase A2; secondary metabolites; seeds; therapeutics;     Show all 18 Subjects
Abstract:
... Therapeutic value of allelochemicals in inflammatory disorders and the potential drug targets need to be elucidated to alleviate tissue and vascular injury. Natural anti-inflammatory agents are known to cause minimal adverse effects. Presence of different secondary metabolites (allelochemicals), protease inhibitors like soap nut trypsin inhibitor (SNTI) from Sapindus trifoliatus and allied compoun ...
DOI:
10.1016/j.ijbiomac.2018.03.139

132. Antioxidant, antimicrobial and antibiotic resistance modifying effect of Heliotropium indicum

Author:
Wani, Parvaze Ahmad; Tolu, Arowolo Mojisola; Wahid, Shazia
Source:
Biocatalysis and agricultural biotechnology 2018 v.15 pp. 113-118
ISSN:
1878-8181
Subject:
trypsin inhibitors,   etc   ; Heliotropium indicum; acetone; additive effect; alkaloids; antibiotic resistance; antimicrobial properties; antioxidant activity; antioxidants; carotenes; ethanol; flavonoids; lutein; oxalates; paper; pathogens; phytic acid; plant extracts; saponins; screening; soil; solvents; synergism;     Show all 23 Subjects
Abstract:
... The present study is based on the determination of phytochemical, antimicrobial, anti-oxidant and antibiotic resistance modifying activities of soil grown Heliotropium indicum. The phytochemical screening showed the presence of Tannin, Oxalate, Phytate, Terpenoids, Trypsin inhibitors, Phenol, Carotenoids, Carotene, Xanthophyll, Flavonoid, Saponin, and Alkaloids. Acetone extracts by paper disc and ...
DOI:
10.1016/j.bcab.2018.05.018

133. Effects of processing on the nutrient composition of rubber seed meal

Author:
Udo, M.D.; Ekpo, U.; Ahamefule, F.O.
Source:
Journal of the Saudi Society of Agricultural Sciences 2018 v.17 no.3 pp. 297-301
ISSN:
1658-077X
Subject:
trypsin inhibitors,   etc   ; Hevea brasiliensis; antinutritional factors; boiling; crude fiber; crude protein; energy; energy content; hydrogen cyanide; livestock; mineral content; minerals; nutrient content; nutritive value; proximate composition; rubber; seeds; toasting;     Show all 18 Subjects
Abstract:
... The nutritive value of raw and processed rubber seed meal (Hevea brasiliensis) was investigated by considering the following parameters – proximate composition, gross energy, mineral composition and anti-nutritional factors. The raw and processed seeds were dried, milled and chemically analysed. Crude protein contents of the raw and processed rubber seeds ranged between 21.08% and 24.60%, while th ...
DOI:
10.1016/j.jssas.2016.06.001

134. Effects of goat milk fractions on the stability of IGF-I in simulated gastrointestinal conditions

Author:
Zhang, Fuxin; Xu, Lianying; Chen, Si; Wang, Bini; Shao, Yuyu; Zhao, Aiqing; Han, Xue
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.91 pp. 229-234
ISSN:
0023-6438
Subject:
trypsin inhibitors,   etc   ; digestion; egg albumen; enzyme-linked immunosorbent assay; gastric juice; gastrointestinal system; goat milk; insulin-like growth factor I; intestinal secretions; lima beans; polyacrylamide gel electrophoresis; proteolysis; soybeans; whey;     Show all 14 Subjects
Abstract:
... The effects of goat milk fractions on the stability of insulin-like growth factor I (IGF-I) digested in the simulated gastrointestinal juice in vitro were investigated. IGF-I concentration was analyzed using an immunological sandwich-type ELISA method. The results showed that the concentration of pure IGF-I in simulated gastrointestinal juice decreased rapidly with the increasing digestion time. H ...
DOI:
10.1016/j.lwt.2018.01.017

135. Molecular Cloning and Structure–Function Analysis of a Trypsin Inhibitor from Tartary Buckwheat and Its Application in Combating Phytopathogenic Fungi

Author:
Ruan, Jing-jun; Tian, Shan-jun; Yan, Jun; Chen, Hui; Xu, Ru-hong; Cheng, Jian-ping
Source:
Agronomy 2018 v.8 no.4
ISSN:
2073-4395
Subject:
trypsin inhibitors,   etc   ; Alternaria solani; DNA; Fagopyrum tataricum; amino acid sequences; amino acids; buckwheat; fungal growth; genome walking; host plants; hyphae; inhibitory concentration 50; insects; molecular cloning; mutants; open reading frames; plant pathogenic fungi; potatoes; proteinases; site-directed mutagenesis; spore germination;     Show all 21 Subjects
Abstract:
... Host plant protease inhibitors offer resistance to proteases from invading pathogens. Trypsin inhibitors (TIs), in particular, serve as protective agents against insect and pathogen attacks. In this study, we designed a pair of degenerate primers based on highly conserved motifs at the N- and C-termini of the TI from tartary buckwheat (Fagopyrum tataricum; Ft) to clone the central portion. Genomic ...
DOI:
10.3390/agronomy8040046

136. Fermentation and enzymatic treatment of pea for turkey nutrition

Author:
Goodarzi Boroojeni, F.; Kozłowski, K.; Jankowski, J.; Senz, M.; Wiśniewska, M.; Boros, D.; Drażbo, A.; Zentek, J.
Source:
Animal feed science and technology 2018 v.237 pp. 78-88
ISSN:
0377-8401
Subject:
trypsin inhibitors,   etc   ; Bacillus licheniformis; Bacillus subtilis; Pisum sativum; alpha-galactosidase; dermatitis; digestibility; dough; enzymatic treatment; experimental diets; fermentation; growth performance; ileum; mash; minerals; nutrients; nutritive value; peas; phytic acid; protein sources; proteinases; resistant starch; spores; turkeys; vitamins;     Show all 25 Subjects
Abstract:
... The present study was conducted to investigate how fermentation (FE) and enzymatic treatment (ET) of pea affect standardized ileal digestibility (SID) of nutrients in diets with peas as the only protein source, and evaluate the consequences of inclusion of different pea products in turkey diets (100 g/kg) on growth performance and bird health. For FE process, Pisum sativum L. was mixed with water ...
DOI:
10.1016/j.anifeedsci.2018.01.008

137. Physico-chemical properties, phytochemicals and DPPH radical scavenging activity of supercritical fluid extruded lentils

Author:
Lv, Ying; Glahn, Raymond P.; Hebb, Richard Lebaron; Rizvi, Syed S.H.
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.89 pp. 315-321
ISSN:
0023-6438
Subject:
trypsin inhibitors,   etc   ; 2,2-diphenyl-1-picrylhydrazyl; ambient temperature; antioxidant activity; antioxidants; brittleness; carbon dioxide; extrusion; flour; gelatinization; hardness; lentils; phytopharmaceuticals; porosity; potato starch; rehydration; retrogradation; starch; water solubility; whole grain foods;     Show all 20 Subjects
Abstract:
... A powder blend comprised of lentil flour and pre-gelatinized potato starch was processed using supercritical fluid (carbon dioxide) extrusion (SCFX). The blend was extruded at temperature of 95–99.5 °C and screw speeds of 40, 80 and 120 rpm. The physical properties of the extruded products such as porosity, rehydration rate, expansion ratio, and crispness—improved as the screw speed increased. Fur ...
DOI:
10.1016/j.lwt.2017.10.063

138. Protective effects of soybean protein and egg white protein on the antibacterial activity of nisin in the presence of trypsin

Author:
Pan, Dan; Zhang, Dong; Hao, Limin; Lin, Songyang; Kang, Qiaozhen; Liu, Xin; Lu, Laizheng; Lu, Jike
Source:
Food chemistry 2018 v.239 pp. 196-200
ISSN:
0308-8146
Subject:
trypsin inhibitors,   etc   ; Listeria monocytogenes; Staphylococcus aureus; antibacterial properties; cooked foods; egg albumen; food pathogens; meat processing; meat products; nisin; plate count; protective effect; soy protein; total volatile basic nitrogen; trypsin;     Show all 15 Subjects
Abstract:
... The using of nisin to prevent foodborne pathogens (Staphylococcus aureus and Listeria monocytogenes) from contamination has received broad attentions during meat processing. However, the application of nisin has been limited because its antibacterial activity may be inhibited by trypsin. In this study, the protective effects of soybean protein and egg white protein on antibacterial activity of nis ...
DOI:
10.1016/j.foodchem.2017.06.091

139. Nutrients and natural toxic substances in commonly consumed Jerusalem artichoke (Helianthus tuberosus L.) tuber

Author:
Judprasong, Kunchit; Archeepsudcharit, Nidthida; Chantapiriyapoon, Kedsiri; Tanaviyutpakdee, Pharrunrat; Temviriyanukul, Piya
Source:
Food chemistry 2018 v.238 pp. 173-179
ISSN:
0308-8146
Subject:
trypsin inhibitors,   etc   ; Helianthus tuberosus; Jerusalem artichokes; cadmium; chemical pollutants; cyanides; dietary fiber; freeze drying; fructans; heavy metals; insecticide residues; iron; lead; nitrates; nitrites; nutrient databanks; nutrients; oxalates; phytic acid; potassium; toxic substances; tubers; Thailand;     Show all 23 Subjects
Abstract:
... This study determined nutrients, chemical contaminants, (insecticide residues and heavy metals), and natural toxic substances (nitrate, nitrite, cyanide, oxalate, phytate, and trypsin inhibitor) in tubers of Jerusalem artichokes—Kaentawan in the Thai language—grown in four major provinces in Thailand. They were purchased, prepared, homogenized, and freeze-dried for further analysis using standard ...
DOI:
10.1016/j.foodchem.2016.09.116

140. Protease treatment of canola meal-containing Japanese quail diets: Effect on physiological parameters and meat quality traits

Author:
Mnisi, Caven Mguvane; Mlambo, Victor
Source:
Journal of applied animal research 2018 v.46 no.1 pp. 1389-1394
ISSN:
0974-1844
Subject:
trypsin inhibitors,   etc   ; Coturnix japonica; adults; body weight; canola; canola meal; carcass characteristics; digestion; feed conversion; feed intake; females; growth performance; heat stability; ingredients; mash; meat quality; poultry feeding; protein sources; proteinases; quails; soybeans; weight gain;     Show all 22 Subjects
Abstract:
... The utility of canola meal as protein source for quails may be limited by trypsin inhibitors that interfere with protein digestion. This study was, therefore, designed to assess the effect of including a heat-stable protease mono-enzyme (75’000 PROT/g; EC/IUB no. 3.4.21) in canola meal-containing diets on physiological and meat quality traits of adult female Japanese quails. A total of 240, five-w ...
DOI:
10.1080/09712119.2018.1516670