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1. Ability of Catalonian donkey sperm to penetrate zona pellucida-free bovine oocytes matured in vitro

Author:
Morató, R.,   et al.   ; Taberner, E.; Mogas, T.; Miró, J.;     Show all 4 Authors
Source:
Animal reproduction science 2010 v.118 no.2-4 pp. 354-361
ISSN:
0378-4320
Subject:
viability; pronucleus; laboratory techniques; cryopreservation; zona pellucida; accuracy; males; sperm motility; velocity; fertilization (reproduction); asses; cows; spermatozoa; thawing; oocytes; cell adhesion; in vitro studies
Abstract:
... An experiment was designed to study the interaction between fresh/frozen-thawed donkey spermatozoa and zona pellucida (ZP)-free bovine oocytes in an attempt to develop a model for assessing cryopreserved Catalonian donkey sperm function. Semen from five donkeys was collected using an artificial vagina. Sperm motility and viability were immediately assessed and the semen sample cryopreserved. Sperm ...
DOI:
10.1016/j.anireprosci.2009.08.005
PubMed:
19748750

2. A CO2-enriched atmosphere improves in vitro growth of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]

Author:
Notini, Marcela Morato,   et al.   ; Saldanha, Cleber Witt; Otoni, Caio Gomide; Kuki, Kacilda Naomi; da Cruz, Ana Cláudia Ferreira; Neto, Aurélio Rubio; Dias, Leonardo Lucas Carnevalli; Otoni, Wagner Campos;     Show all 8 Authors
Source:
In vitro cellular & developmental biology 2013 v.49 no.4 pp. 433-444
ISSN:
1054-5476
Subject:
carbon dioxide; growth and development; carbon dioxide enrichment; sucrose; Pfaffia glomerata; water content; ecdysterone; culture media; leaves; pigments
Abstract:
... The aim of the present study was to evaluate the effects of forced ventilation and CO₂enrichment (360 or 720 μmol mol⁻¹CO₂) on the in vitro growth and development of Pfaffia glomerata, an endangered medicinal species, under photomixotrophic or photoautotrophic conditions. P. glomerata nodal segments showed substantial differences in growth, relative water content and water loss from leaves, photos ...
DOI:
10.1007/s11627-013-9529-5

3. A comprehensive analysis of autocorrelation and bias in home range estimation

Author:
Morato, Ronaldo G.,   et al.   ; Noonan, Michael J.; Tucker, Marlee A.; Fleming, Christen H.; Akre, Thomas S.; Alberts, Susan C.; Ali, Abdullahi H.; Altmann, Jeanne; Antunes, Pamela Castro; Belant, Jerrold L.; Beyer, Dean; Blaum, Niels; Böhning‐Gaese, Katrin; Cullen, Laury, Jr.; de Paula, Rogerio Cunha; Dekker, Jasja; Drescher‐Lehman, Jonathan; Farwig, Nina; Fichtel, Claudia; Fischer, Christina; Ford, Adam T.; Goheen, Jacob R.; Janssen, René; Jeltsch, Florian; Kauffman, Matthew; Kappeler, Peter M.; Koch, Flávia; LaPoint, Scott; Markham, A. Catherine; Medici, Emilia Patricia; Nathan, Ran; Oliveira‐Santos, Luiz Gustavo R.; Olson, Kirk A.; Patterson, Bruce D.; Paviolo, Agustin; Ramalho, Emiliano Esterci; Rösner, Sascha; Schabo, Dana G.; Selva, Nuria; Sergiel, Agnieszka; Xavier da Silva, Marina; Spiegel, Orr; Thompson, Peter; Ullmann, Wiebke; Zięba, Filip; Zwijacz‐Kozica, Tomasz; Fagan, William F.; Mueller, Thomas; Calabrese, Justin M.;     Show all 49 Authors
Source:
Ecological monographs 2019 v.89 no.2 pp. e01344
ISSN:
0012-9615
Subject:
animals; autocorrelation; data collection; global positioning systems; home range; least squares
Abstract:
... Home range estimation is routine practice in ecological research. While advances in animal tracking technology have increased our capacity to collect data to support home range analysis, these same advances have also resulted in increasingly autocorrelated data. Consequently, the question of which home range estimator to use on modern, highly autocorrelated tracking data remains open. This questio ...
DOI:
10.1002/ecm.1344

4. Activin‐A receptor expression patterns in prepubertal goat oocytes and derived embryos

Author:
Morató, Roser,   et al.   ; Hammami, Sondes; Paramio, Maria Teresa; Izquierdo, Dolors;     Show all 4 Authors
Source:
Reproduction in domestic animals 2019 v.54 no.5 pp. 804-807
ISSN:
0936-6768
Subject:
Western blotting; blastocyst; embryogenesis; goats; immunocytochemistry; oocytes; puberty; receptors
Abstract:
... This study examines the presence of activin IIA and IIB receptors (ActR‐IIA and ActR‐IIB) by Western blotting and immunocytochemistry in immature and IVM‐oocytes, 2 to 8‐cells embryos and blastocysts from prepubertal goats. Western blotting revealed that activin receptors are synthesized during oocyte maturation and embryo development. In the immunocytochemistry experiments, no immunostaining for ...
DOI:
10.1111/rda.13416

5. Addition of l-ascorbic acid to culture and vitrification media of IVF porcine blastocysts improves survival and reduces HSPA1A levels of vitrified embryos

Author:
Morató, Roser,   et al.   ; Castillo-Martín, Miriam; Yeste, Marc; Soler, Albert; Bonet, Sergi;     Show all 5 Authors
Source:
Reproduction, fertility, and development 2015 v.27 no.7 pp. 1115-1123
ISSN:
1031-3613
Subject:
DNA fragmentation; ascorbic acid; blastocyst; culture media; gene expression; heat-shock protein 70; in vitro fertilization; survival rate; swine; vitrification
Abstract:
... The aim of the present study was to determine the effect of l-ascorbic acid on embryo quality and gene expression of porcine blastocysts after supplementations of in vitro culture medium and/or vitrification–warming media. Embryo quality, in terms of total cell number (TCN), DNA fragmentation and peroxide levels, together with the relative transcript abundance of BCL-2 associated X protein (BAX), ...
DOI:
10.1071/RD14078

6. A dipeptide and an amino acid present in whey protein hydrolysate increase translocation of GLUT-4 to the plasma membrane in Wistar rats

Author:
Morato, P.N.,   et al.   ; Lollo, P.C.B.; Moura, C.S.; Batista, T.M.; Carneiro, E.M.; Amaya-Farfan, J.;     Show all 6 Authors
Source:
Food chemistry 2013 v.139 no.1-4 pp. 853-859
ISSN:
0308-8146
Subject:
Western blotting; amino acids; blood glucose; free amino acids; glucose; glucose transporters; glycogen; plasma membrane; protein hydrolysates; rats; skeletal muscle; whey protein
Abstract:
... Whey protein hydrolysate (WPH) is capable of increasing muscle glycogen reserves and of concentrating the glucose transporter in the plasma membrane (PM). The objective of this study was to determine which WPH components could modulate translocation of the glucose transporter GLUT-4 to the PM of animal skeletal muscle. Forty-nine animals were divided into 7 groups (n=7) and received by oral gavage ...
DOI:
10.1016/j.foodchem.2012.12.062
PubMed:
23561181

7. A global assessment of seamount ecosystems knowledge using an ecosystem evaluation framework

Author:
Morato, Telmo,   et al.   ; Kvile, Kristina Ø.; Taranto, Gerald H.; Pitcher, Tony J.;     Show all 4 Authors
Source:
Biological conservation 2014 v.173 pp. 108-120
ISSN:
0006-3207
Subject:
Internet; basins; benthic organisms; databases; ecosystems; food webs; geophysics; habitats; indigenous species; natural resources conservation; scientists; Europe
Abstract:
... Seamounts are ubiquitous habitats of the deep-sea, collectively forming an area as large as Europe. Their characteristics have led marine scientists to hypothesize a series of ‘seamount effects’ enhancing numbers of endemic species, rates of production and bentho-pelagic trophic transfers. These effects have profound implications for deep-sea management; collating the existing body of seamount kno ...
DOI:
10.1016/j.biocon.2013.10.002

8. Amphiphilic ionic complexes of hyaluronic acid with organophosphonium compounds and their antimicrobial activity

Author:
Morató, Jordi,   et al.   ; Gamarra, Ana; Forés, Eva; Muñoz-Guerra, Sebastián;     Show all 4 Authors
Source:
International journal of biological macromolecules 2018
ISSN:
0141-8130
Subject:
Escherichia coli; antimicrobial properties; biocides; biodegradation; fungi; heat; hyaluronic acid; liquids; melting; periodicity; soaps; temperature
Abstract:
... Amphiphilic ionic complexes of hyaluronic acid and alkyltrimethylphosphonium soaps with alkyl chains containing even numbers of carbons from 12 to 22 have been produced. The complexes have a nearly stoichiometric composition, are non-water soluble, and are stable to heat up to temperatures above 200 °C. These complexes are amphiphilic and able to adopt a biphasic structure with the paraffinic and ...
DOI:
10.1016/j.ijbiomac.2018.07.077

9. A multicentric study to evaluate the use of relative retention times in targeted proteomics

Author:
Morato-López, Esperanza,   et al.   ; Vialas, Vital; Colomé-Calls, Núria; Abian, Joaquín; Aloria, Kerman; Alvarez-Llamas, Gloria; Antúnez, Oreto; Arizmendi, Jesus M.; Azkargorta, Mikel; Barceló-Batllori, Silvia; Barderas, María G.; Blanco, Francisco; Casal, J. Ignacio; Casas, Vanessa; de la Torre, Carolina; Chicano-Gálvez, Eduardo; Elortza, Felix; Espadas, Guadalupe; Estanyol, Josep M.; Fernandez-Irigoyen, Joaquín; Fernandez-Puente, Patricia; Fidalgo, María José; Fuentes, Manuel; Gay, Marina; Gil, Concha; Hainard, Alexandre; Hernaez, Maria Luisa; Ibarrola, Nieves; Kopylov, Arthur T.; Lario, Antonio; Lopez, Juan Antonio; López-Lucendo, María; Marcilla, Miguel; Marina-Ramírez, Anabel; Marko-Varga, Gyorgy; Martín, Luna; Mora, Maria I.; Muñoz, Javier; Odena, Maria Antonia; de Oliveira, Eliandre; Orera, Irene; Ortea, Ignacio; Pasquarello, Carla; Ray, Kevin B.; Rezeli, Melinda; Ruppen, Isabel; Sabidó, Eduard; del Pino, Manuel M. Sanchez; Sancho, Jaime; Santamaría, Enrique; Vazquez, Jesus; Vilaseca, Marta; Vivanco, Fernando; Walters, James J.; Zgoda, Victor G.; Corrales, Fernando J.; Canals, Francesc; Paradela, Alberto;     Show all 58 Authors
Source:
Journal of proteomics 2017 v.152 pp. 138-149
ISSN:
1874-3919
Subject:
chromatography; monitoring; proteomics; quantitative analysis; reproducibility
Abstract:
... Despite the maturity reached by targeted proteomic strategies, reliable and standardized protocols are urgently needed to enhance reproducibility among different laboratories and analytical platforms, facilitating a more widespread use in biomedical research. To achieve this goal, the use of dimensionless relative retention times (iRT), defined on the basis of peptide standard retention times (RT) ...
DOI:
10.1016/j.jprot.2016.10.014

10. Analysis of free hydroxytyrosol in human plasma following the administration of olive oil A

Author:
Rodríguez-Morató, Jose,   et al.   ; Pastor, Antoni; Olesti, Eulàlia; Pujadas, Mitona; Pérez-Mañá, Clara; Khymenets, Olha; Fitó, Montserrat; Covas, María-Isabel; Solá, Rosa; Motilva, María-José; Farré, Magí; de la Torre, Rafael;     Show all 12 Authors
Source:
Journal of chromatography 2016 v.1437 pp. 183-190
ISSN:
0021-9673
Subject:
bioactive properties; bioavailability; clinical trials; derivatization; extra-virgin olive oil; humans; ingestion; liquid chromatography; pharmacokinetics; polyphenols; solid phase extraction; tandem mass spectrometry
Abstract:
... Hydroxytyrosol (HT) from olive oil, a potent bioactive molecule with health benefits, has a poor bioavailability, its free form (free HT) being undetectable so far. This fact leads to the controversy whether attained HT concentrations after olive oil polyphenol ingestion are too low to explain the observed biological activities. Due to this, an analytical methodology to determine free HT in plasma ...
DOI:
10.1016/j.chroma.2016.02.016

11. A new microtitre plate screening method for evaluating the viability of aerobic respiring bacteria in high surface biofilms

Author:
Morató, J.,   et al.   ; Pérez, L.M.; Álvarez, B.L.; Codony, F.; Fittipaldi, M.; Adrados, B.; Peñuela, G.;     Show all 7 Authors
Source:
Letters in applied microbiology 2010 v.51 no.3 pp. 331-337
ISSN:
0266-8254
Subject:
antibacterial properties; bacteria; biofilm; cell viability; colorimetry; metabolism; microbial biomass; microbial communities; salts; screening; tetrazolium
Abstract:
... It is difficult to determine the effects of bactericidal compounds against bacteria in a biofilm because classical procedures for determining cell viability require several working days, multiple complicated steps and are frequently only applicable to cells in suspension. We attempt to develop a compact, inexpensive and versatile system to measure directly the extent of biofilm formation from wate ...
DOI:
10.1111/j.1472-765X.2010.02902.x
PubMed:
20681967

12. A New Species of Ameivula (Squamata, Teiidae) from Southern Espinhaço Mountain Range, Brazil

Author:
de Carvalho, Celso Morato,   et al.   ; Arias, Federico; Zaher, Hussam; Rodrigues, Miguel Trefaut;     Show all 4 Authors
Source:
Copeia 2014 v.2014 no.1 pp. 95-105
ISSN:
0045-8511
Subject:
Squamata; altitude; granules; mountains; new genus; new species; phylogeny; Brazil
Abstract:
... In a recent phylogenetic analysis of Teiidae based on morphological characters, three new genera were erected to remove polyphyletism in the genus Cnemidophorus. Accordingly, Ameivula was proposed to accommodate the species formerly included in the Cnemidophorus ocellifer group, characterized by the presence of granules in the supraocular semicircles, femoral pores fewer than 40, and preanal spurs ...
DOI:
10.1643/CH-13-037

13. An overview of fisheries discards in the Azores

Author:
Morato, T.,   et al.   ; Fauconnet, L.; Pham, C.K.; Canha, A.; Afonso, P.; Diogo, H.; Machete, M.; Silva, H.M.; Vandeperre, F.;     Show all 9 Authors
Source:
Fisheries research 2019 v.209 pp. 230-241
ISSN:
0165-7836
Subject:
aquacultural and fisheries equipment; bycatch; environmental impact; sharks; traditional technology; Azores
Abstract:
... In contrast to the vast majority of European maritime territories, the Azores is an isolated oceanic archipelago in the North East Atlantic characterised by small-scale hooks-and-lines fisheries. Yet, in spite of the low environmental impact of such fishing gears, there is no solid integrative information on the discard practices. This study bridges this gap by providing a comprehensive, historic ...
DOI:
10.1016/j.fishres.2018.10.001

14. An overview of proximate factors affecting the nesting behavior of solitary wasps and bees (Hymenoptera: Aculeata) in preexisting cavities in wood

Author:
Morato, E.F.,   et al.   ; Martins, R.P.;     Show all 2 Author
Source:
Neotropical entomology 2006 v.35 no.3 pp. 285-298
ISSN:
1519-566X
Subject:
Hymenoptera; solitary bees; nesting; nesting sites; wood; plant-insect relations
Abstract:
... cavities in wood are important components of terrestrial ecosystems because they engage in several ecological interactions (e.g. predation and pollination) with other species of plants and animals. Spatial and temporal variations in richness and abundance of solitary wasps and bees can be related to changes in environmental structure and in the diversity of other groups of organisms. The nesting p ...
DOI:
10.1590/S1519-566X2006000300001
PubMed:
18575687

15. Anti-inflammatory effect of bee venom on antigen-induced arthritis in rabbits: Influence of endogenous glucocorticoids

Author:
Castro, Fábio Morato,   et al.   ; Saad Rached, Izabella Cordeiro Freire; Guzzo, Maria Luiza; de Mello, Suzana Beatriz Veríssimo;     Show all 4 Authors
Source:
Journal of ethnopharmacology 2010 v.130 no.1 pp. 175-178
ISSN:
0378-8741
Subject:
rabbits; mechanism of action; leukocyte count; venoms; anti-inflammatory activity; arthritis; Apoidea; leukocytes; animal models; bites and stings; prostaglandins; cell membranes; synovial sheaths; endogenous sources; glucocorticoids; synovial fluid; protective effect
Abstract:
... Aim of the study: This study assessed the involvement of endogenous glucocorticoids (GCs) in the anti-arthritic properties of bee venom (BV) on antigen-induced arthritis (AIA) in rabbits. Materials and methods: BV (1.5-6μg/kg/day) was injected for 7 days before AIA induction, whereas the control group received sterile saline. The total and differential leukocyte count, PGE₂ levels in synovial flui ...
DOI:
10.1016/j.jep.2010.04.015
PubMed:
20457243

16. A perspective on the importance of oceanic fronts in promoting aggregation of visitors to seamounts

Author:
Morato, Telmo,   et al.   ; Miller, Peter I; Dunn, Daniel C; Nicol, Simon J; Bowcott, James; Halpin, Patrick N;     Show all 6 Authors
Source:
Fish and fisheries 2016 v.17 no.4 pp. 1227-1233
ISSN:
1467-2960
Subject:
bioactive properties; biodiversity; biomass; data collection; environmental factors; seamounts; tuna; Pacific Ocean
Abstract:
... Recent evidence has demonstrated that not all seamounts are areas where productivity, biomass and biodiversity of marine life thrive. Therefore, understanding the drivers and mechanisms underlying seamount productivity is a major challenge in today's seamount research. Incorporating oceanographic data in future analyses has been suggested to be of paramount importance to unveil many of the seamoun ...
DOI:
10.1111/faf.12126

17. Aquaglyceroporins 3 and 7 in bull spermatozoa: identification, localisation and their relationship with sperm cryotolerance

Author:
Morató, Roser,   et al.   ; Prieto-Martínez, Noelia; Muiño, Rodrigo; Hidalgo, Carlos O.; Rodríguez-Gil, Joan E.; Bonet, Sergi; Yeste, Marc;     Show all 7 Authors
Source:
Reproduction, fertility, and development 2017 v.29 no.6 pp. 1249-1259
ISSN:
1031-3613
Subject:
bulls; cryopreservation; immunoblotting; immunocytochemistry; regression analysis; semen; sperm quality; thawing
Abstract:
... The present study aimed to determine the localisation of aquaglyceroporins 3 (AQP3) and 7 (AQP7) in bull spermatozoa and their relationship with the sperm cell’s resilience to withstand cryopreservation (i.e. cryotolerance). A total of 18 bull ejaculates were cryopreserved and their sperm quality analysed before and after freeze–thawing. The presence and localisation of AQP3 and AQP7 was determine ...
DOI:
10.1071/RD16077

18. Aquaporins 7 and 11 in boar spermatozoa: detection, localisation and relationship with sperm quality

Author:
Morató, Roser,   et al.   ; Prieto-Martínez, Noelia; Vilagran, Ingrid; Rodríguez-Gil, Joan E.; Yeste, Marc; Bonet, Sergi;     Show all 6 Authors
Source:
Reproduction, fertility, and development 2016 v.28 no.6 pp. 663-672
ISSN:
1031-3613
Subject:
Western blotting; aquaporins; boars; cell membranes; immunocytochemistry; permeability; solutes; sperm motility; spermatozoa
Abstract:
... Aquaporins (AQPs) are integral membrane water channels that allow transport of water and small solutes across cell membranes. Although water permeability is known to play a critical role in mammalian cells, including spermatozoa, little is known about their localisation in boar spermatozoa. Two aquaporins, AQP7 and AQP11, in boar spermatozoa were identified by western blotting and localised throug ...
DOI:
10.1071/RD14237

19. Aquaporins in boar spermatozoa. Part II: detection and localisation of aquaglyceroporin 3

Author:
Morató, Roser,   et al.   ; Prieto-Martínez, Noelia; Vilagran, Ingrid; Rodríguez-Gil, Joan E.; Bonet, Sergi; Yeste, Marc;     Show all 6 Authors
Source:
Reproduction, fertility, and development 2017 v.29 no.4 pp. 703-711
ISSN:
1031-3613
Subject:
Western blotting; aquaporins; boars; fluorescent antibody technique; solutes; somatic cells; sperm quality; staining
Abstract:
... The proteins belonging to the aquaporin family play a fundamental role in water and solute transport across biological membranes. While the presence of these proteins has been extensively studied in somatic cells, their function in mammalian spermatozoa has been studied less. The present study was designed to identify and localise aquaglyceroporin 3 (AQP3) in boar spermatozoa. With this purpose, 2 ...
DOI:
10.1071/RD15164

20. Aquaporins in the male reproductive tract and sperm: Functional implications and cryobiology

Author:
Morató, R,   et al.   ; Yeste, M; Rodríguez‐Gil, JE; Bonet, S; Prieto‐Martínez, N;     Show all 5 Authors
Source:
Reproduction in domestic animals 2017 v.52 Suppl S4 pp. 12-27
ISSN:
0936-6768
Subject:
absorption; aquaporins; cell membranes; cold tolerance; cryopreservation; epididymis; females; males; mammals; oocytes; osmolality; secretion; sequence homology; solutes; sperm transport; spermatids; spermatogenesis; spermatozoa; testes
Abstract:
... Aquaporins (AQPs) play a vital role for the transport of water and solutes across cell membranes. Classification of these ubiquitous proteins into three categories (orthodox AQPs, aquaglyceroporins and superaquaporins) is based on their sequence similarity and substrate selectivity. In the male reproductive tract of mammals, most AQPs (except AQP6 and AQP12) are found in different organs (includin ...
DOI:
10.1111/rda.13082