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1. Glycosyldiacylglycerolipids from the lichen Dictyonema glabratum

Author:
Iacomini, M.,   et al.   ; Sassaki, G.L.; Machado, M.J.; Tischer, C.A.; Gorin, P.A.J.;     Show all 5 Authors
Source:
Journal of natural products 1999 v.62 no.6 pp. 844-847
ISSN:
0163-3864
Subject:
lichens; spectral analysis; chemical structure; chemical constituents of plants; Brazil
DOI:
10.1021/np980547f
PubMed:
10395500

2. Isolation and characterization of a galactorhamnan polysaccharide from the seed coat of Canavalia ensiformis that is toxic to the cowpea weevil (Callosobruchus maculatus)

Author:
Iacomini, M.,   et al.   ; Oliveira, A.E.A.; Sassaki, G.L.; Cunha, M. da.; Gomes, V.M.; Fernandes, B.K.V.S.; Xavier-Filho, J.;     Show all 7 Authors
Source:
Entomologia experimentalis et applicata 2001 v.101 no.3 pp. 225-231
ISSN:
0013-8703
Subject:
Canavalia ensiformis; galactose; rhamnose; Callosobruchus maculatus; mortality; toxicity; polysaccharides; storage pests; biological development; pest resistance; chemical structure; larvae
Abstract:
... We have isolated a water-soluble polysaccharide from the Jack bean [Canavalia ensiformis (L) DC] seed coat that was shown to be highly detrimental to larval development of the cowpea weevil (Callosobruchus maculatus) (Coleoptera: Bruchidae). Determination of the composition and structure of this polysaccharide showed that it is a galactorhamnan with an M(w) of 883.0, containing 92% rhamnose and 8% ...
DOI:
10.1046/j.1570-7458.2001.00907.x

3. Chemical and biological properties of an arabinogalactan from Phyllanthus niruri

Author:
Iacomini, M.,   et al.   ; Mellinger, C.G.; Carbonero, E.R.; Noleto, G.R.; Cipriani, T.R.; Oliveira, M.B.M.; Gorin, P.A.J.;     Show all 7 Authors
Source:
Journal of natural products 2005 v.68 no.10 pp. 1479-1483
ISSN:
0163-3864
Subject:
Phyllanthus niruri; herbaceous plants; medicinal plants; traditional medicine; plant extracts; phytochemicals; arabinogalactans; chemical structure; spectral analysis; mice; macrophages
DOI:
10.1021/np050129s
PubMed:
16252911

4. Xylans from the medicinal herb Phyllanthus niruri

Author:
Iacomini, M.,   et al.   ; Mellinger, C.G.; Carbonero, E.R.; Cipriani, T.R.; Gorin, P.A.J.;     Show all 5 Authors
Source:
Journal of natural products 2005 v.68 no.1 pp. 129-132
ISSN:
0163-3864
Subject:
xylan; Phyllanthus niruri; herbaceous plants; medicinal plants; carbohydrate structure; spectral analysis
DOI:
10.1021/np049778e
PubMed:
15679336

5. A β -glucan from the fruit bodies of edible mushrooms Pleurotus eryngii and Pleurotus ostreatoroseus

Author:
Iacomini, M.,   et al.   ; Carbonero, E.R.; Gracher, A.H.P.; Smiderle, F.R.; Rosado, F.R.; Sassaki, G.L.; Gorin, P.A.J.;     Show all 7 Authors
Source:
Carbohydrate polymers 2006 v.66 no.2 pp. 252-257
ISSN:
0144-8617
Subject:
functional foods; chemical composition; fractionation; nuclear magnetic resonance spectroscopy; methylation; freeze-thaw cycles; Pleurotus eryngii; mushrooms; degradation; hot water treatment; beta-glucans; fruiting bodies; chemical structure; Pleurotus ostreatus
Abstract:
... The glucans of basidiomycetes are an important class of polysaccharides with potential biological activities. In this work, the β-glucans were isolated from the fruiting bodies of edible mushrooms, Pleurotus eryngii and Pleurotus ostreatoroseus, via extraction with hot water, and then fractionation by freeze-thawing. The insoluble glucans gave similar 13C NMR spectra, monosaccharide composition an ...
DOI:
10.1016/j.carbpol.2006.03.009

6. Culture conditions for the production of an acidic exopolysaccharide by the nitrogen-fixing bacterium Burkholderia tropica

Author:
Iacomini, M.,   et al.   ; Serrato, R.V.; Sassaki, G.L.; Cruz, L.M.; Pedrosa, F.O.; Gorin, P.A.J.;     Show all 6 Authors
Source:
Canadian journal of microbiology 2006 v.52 no.5 pp. 489-493
ISSN:
0008-4166
Subject:
Burkholderia; nitrogen-fixing bacteria; carbohydrate metabolism; polysaccharides; cell culture; culture media; carbon nitrogen ratio; endophytes
DOI:
10.1139/W05-155
PubMed:
16699575

7. Nitric oxide-dependent vasorelaxation induced by extractive solutions and fractions of Maytenus ilicifolia Mart ex Reissek (Celastraceae) leaves

Author:
Iacomini, M.,   et al.   ; Rattmann, Y.D.; Cipriani, T.R.; Sassaki, G.L.; Rieck, L.; Marques, M.C.A.; Silva-Santos, J.E. da;     Show all 7 Authors
Source:
Journal of ethnopharmacology 2006 v.104 no.3 pp. 328-335
ISSN:
0378-8741
Subject:
Maytenus; medicinal plants; leaves; chemical constituents of plants; plant extracts; medicinal properties; nitric oxide; vasodilation; aorta; bioassays; rats
Abstract:
... This study reveals that an ethanolic supernatant obtained from an aqueous extractive solution prepared from residues of methanolic extracts of ground leaves of Maytenus ilicifolia is able to cause a concentration- and endothelium-dependent relaxation in pre-contract rat aorta rings, with EC50 of 199.7 (190-210) μg/ml. The non-selective nitric oxide synthase inhibitors l-NAME and l-NMMA abolished t ...
DOI:
10.1016/j.jep.2005.09.026

8. A Polysaccharide from a Tea (Infusion) of Maytenus ilicifolia Leaves with Anti-ulcer Protective Effects

Author:
Iacomini, M.,   et al.   ; Cipriani, T.R.; Mellinger, C.G.; Souza, L.M. de; Baggio, C.H.; Freitas, C.S.; Marques, M.C.A.; Gorin, P.A.J.; Sassaki, G.L.;     Show all 9 Authors
Source:
Journal of natural products 2006 v.69 no.7 pp. 1018-1021
ISSN:
0163-3864
Subject:
Celastraceae; medicinal plants; polysaccharides; carbohydrate structure; spectral analysis; rats; stomach ulcers; anti-ulcer activity
DOI:
10.1021/np060045z
PubMed:
16872136

9. Structural characterization of a polysaccharide and a β-glucan isolated from the edible mushroom Flammulina velutipes

Author:
Iacomini, M.,   et al.   ; Smiderle, F.R.; Carbonero, E.R.; Mellinger, C.G.; Sassaki, G.L.; Gorin, P.A.J.;     Show all 6 Authors
Source:
Phytochemistry 2006 v.67 no.19 pp. 2189-2196
ISSN:
0031-9422
Subject:
glycosidic linkages; monosaccharides; methylation; glucans; Flammulina velutipes; carbohydrate composition; spectral analysis; mushrooms
Abstract:
... Two polysaccharides were isolated from the basidiomycete Flammulina velutipes, via successive hot extraction with water, 2% and 25% aq. KOH, and then submitted to freeze-drying. The precipitate formed by repeated freeze-thawing from the 2% aq. KOH extraction PK2 was analyzed by determination of its monosaccharide composition, as well as by methylation analyses using GC-MS, mono- (13C, 1H NMR) and ...
DOI:
10.1016/j.phytochem.2006.06.022

10. Cashew-nut tree exudate gum: Identification of an arabinogalactan-protein as a constituent of the gum and use on the stimulation of somatic embryogenesis

Author:
Iacomini, M.,   et al.   ; Pereira-Netto, A.B.; Pettolino, F.; Cruz-Silva, C.T.A.; Simas, F.F.; Bacic, A.; Carneiro-Leão, A.M. dos A.; Maurer, J.B.B.;     Show all 8 Authors
Source:
Plant science 2007 v.173 no.4 pp. 468-477
ISSN:
0168-9452
Subject:
plant exudates; cashew nuts; polyacrylamide gel electrophoresis; somatic embryogenesis; gel chromatography; amino acid composition; reversed-phase high performance liquid chromatography; proteoglycans; protein content; nut trees; Anacardium occidentale; plant proteins
DOI:
10.1016/j.plantsci.2007.07.008

11. Flavonoid-rich fraction of Maytenus ilicifolia Mart. ex. Reiss protects the gastric mucosa of rodents through inhibition of both H+,K+-ATPase activity and formation of nitric oxide

Author:
Iacomini, M.,   et al.   ; Baggio, C.H.; Freitas, C.S.; Otofuji, G. de M.; Cipriani, T.R.; Souza, L.M. de; Sassaki, G.L.; Marques, M.C.A.; Mesia-Vela, S.;     Show all 9 Authors
Source:
Journal of ethnopharmacology 2007 v.113 no.3 pp. 433-440
ISSN:
0378-8741
Subject:
epicatechin; enzyme inactivation; rabbits; hypersecretion; medicinal plants; plant extracts; protective effect; glutathione; gastric acid; pylorus; medicinal properties; galactitol; leaves; lesions (animal); acute course; chronic diseases; Maytenus; rats; catechin; enzyme activity; nitric oxide; gastric mucosa; animal models; mucus
DOI:
10.1016/j.jep.2007.06.015

12. Structure of a glycoglucuronomannan from the gum exudate of Vochysia tucanorum (family Vochysiaceae)

Author:
Iacomini, M.,   et al.   ; Wagner, R.; Simas, F.F.; Pereira, G.C.Z.; Angeli, A.; Brito, J.O.; Woranovicz-Barreira, S.M.; Delgobo, C.L.; Sassaki, G.L.; Gorin, P.A.J.;     Show all 10 Authors
Source:
Carbohydrate polymers 2007 v.69 no.3 pp. 512-521
ISSN:
0144-8617
Subject:
plant exudates; xylan; Vochysiaceae; mannans; trees; carbohydrate structure; oligosaccharides; chemotaxonomy
Abstract:
... The polysaccharide (VTP) from the gum exudate of Vochysia tucanorum gave aqueous solutions of low viscosity. VTP contained two components, which on freeze-thawing, gave rise to soluble (S-VTP) and precipitated (P-VTP) fractions, with Mw 350,000 and 520,000 g/mol, and dn/dc 0.134 and 0.139, respectively. Acid hydrolysis of S-VTP provided Ara, Xyl, Man, Gal, Glc in a 43:1:5:18:2 molar ratio (GC-MS) ...
DOI:
10.1016/j.carbpol.2007.01.005

13. A 3-O-methylated mannogalactan from Pleurotus pulmonarius: Structure and antinociceptive effect

Author:
Iacomini, M.,   et al.   ; Smiderle, F.R.; Olsen, L.M.; Marcon, R.; Baggio, C.H.; Freitas, C.S.; Santos, A.R.S.; Torri, G.; Gorin, P.A.J.;     Show all 9 Authors
Source:
Phytochemistry 2008 v.69 no.15 pp. 2731-2736
ISSN:
0031-9422
Subject:
anti-inflammatory activity; galactans; Pleurotus pulmonarius; muscle contraction; monosaccharides; methylation; smooth muscle; human health; mice; mushrooms; chemical structure; analgesic effect
Abstract:
... A polysaccharide (M(w) 2.39 x 10⁴ g/mol) was extracted with cold water from the basidiomycete Pleurotus pulmonarius, and its antinociceptive and anti-inflammatory properties were evaluated. It was a mannogalactan (MG), whose structure was characterized using mono- and two-dimensional NMR spectroscopy, methylation analysis, and a controlled Smith degradation. It had a main chain of (1 to 6)-linked ...
DOI:
10.1016/j.phytochem.2008.08.006

14. Comparison of structure of gum exudate polysaccharides from the trunk and fruit of the peach tree (Prunus persica)

Author:
Iacomini, M.,   et al.   ; Simas, F.F.; Gorin, P.A.J.; Wagner, R.; Sassaki, G.L.; Bonkerner, A.;     Show all 6 Authors
Source:
Carbohydrate polymers 2008 v.71 no.2 pp. 218-228
ISSN:
0144-8617
Subject:
plant exudates; chemical composition; acetates; monosaccharides; methylation; chemical analysis; tree trunk; alditols; Prunus persica; arabinogalactans; fruit trees; chemical structure
Abstract:
... The peach tree (Prunus persica) produces gum exudates on its trunk and fruit. The former was extracted with water to give polysaccharide (PPN) and residual material was extracted with alkali giving structurally similar PPNA (NMR examination), which was formed in a slightly higher yield (42% compared with 37%). PPNA consisted of Ara, Xyl, Man, Gal, and uronic acids in a 36:7:2:42:13 molar ratio and ...
DOI:
10.1016/j.carbpol.2007.05.032

15. Lentinus edodes heterogalactan: Antinociceptive and anti-inflammatory effects

Author:
Iacomini, M.,   et al.   ; Carbonero, E.R.; Gracher, A.H.P.; Komura, D.L.; Marcon, R.; Freitas, C.S.; Baggio, C.H.; Santos, A.R.S.; Torri, G.; Gorin, P.A.J.;     Show all 10 Authors
Source:
Food chemistry 2008 v.111 no.3 pp. 531-537
ISSN:
0308-8146
Subject:
acetic acid; anti-inflammatory activity; galactans; dose response; Lentinula edodes; mushrooms; chemical structure; analgesic effect
Abstract:
... Cold aqueous extraction of basidiocarps (fruiting bodies) of the edible mushroom Lentinus edodes (shiitake) gave rise to a heteropolysaccharide, whose chemical structure, antinociceptive and anti-inflammatory properties were determined. Its chemical structure was based on monosaccharide composition, methylation analysis, and NMR spectroscopy (¹H, ¹³C, HSQC, HSQC-TOCSY, HSQC-NOESY, and coupled HMQC ...
DOI:
10.1016/j.foodchem.2008.04.015

16. Structural characterization of an acidic exoheteropolysaccharide produced by the nitrogen-fixing bacterium Burkholderia tropica

Author:
Iacomini, M.,   et al.   ; Serrato, R.V.; Sassaki, G.L.; Gorin, P.A.J.; Cruz, L.M.; Pedrosa, F.O.; Choudhury, B.; Carlson, R.W.;     Show all 8 Authors
Source:
Carbohydrate polymers 2008 v.73 no.4 pp. 564-572
ISSN:
0144-8617
Subject:
Burkholderia; biopolymers; chemical structure; polysaccharides; soil bacteria; nitrogen-fixing bacteria
Abstract:
... An acidic exopolysaccharide (EPS) produced by the diazotrophic bacterium Burkholderia tropica, strain Ppe8, was isolated from the culture supernatant of bacteria grown in a synthetic liquid medium containing mannitol and glutamate. Monosaccharide composition showed Rha, Glc and GlcA in a 2.0:2.0:1.0 molar ratio, respectively. Further structural characterization was performed by a combination of NM ...
DOI:
10.1016/j.carbpol.2007.12.027
PubMed:
26048222

17. Unusual partially 3-O-methylated α-galactan from mushrooms of the genus Pleurotus

Author:
Iacomini, M.,   et al.   ; Carbonero, E.R.; Gracher, A.H.P.; Rosa, M.C.C.; Torri, G.; Sassaki, G.L.; Gorin, P.A.J.;     Show all 7 Authors
Source:
Phytochemistry 2008 v.69 no.1 pp. 252-257
ISSN:
0031-9422
Subject:
galactans; glycosidic linkages; monosaccharides; methylation; Pleurotus djamor; Pleurotus eryngii; spectral analysis; mushrooms
Abstract:
... Indistinguishable partially 3-O-methylated galactans were isolated from the edible basidiomycetes Pleurotus eryngii and Pleurotus ostreatoroseus. They were obtained via successive aqueous extraction, freeze-thawing, precipitation with Fehling solution of soluble material, and ultrafiltration. Mono- and bidimensional 13C and 1H-nuclear magnetic resonance spectroscopy (HMBC, HETEROTOCSY, COSY, and H ...
DOI:
10.1016/j.phytochem.2007.06.018

18. A high-viscosity glycoglucuronomannan from the gum exudate of Vochysia thyrsoidea: Comparison with those of other Vochysia spp

Author:
Iacomini, M.,   et al.   ; Wagner, R.; Simas, F.F.; Sassaki, G.L.; Silva, M.A. da; Gorin, P.A.J.;     Show all 6 Authors
Source:
Carbohydrate polymers 2008 v.72 no.3 pp. 382-389
ISSN:
0144-8617
Subject:
plant exudates; solutions; biopolymers; mannans; viscosity; chemical structure; Vochysia; chemotaxonomy
Abstract:
... The polysaccharide (VTh) extracted with water from the gum exudate of the trunk Vochysia thyrsoidea gave a solution of high viscosity and was homogeneous, with Mw 92.5 x 10³ and dn/dc = 0.212. Partial hydrolysis gave a main chain of to 4)-β-d-GlcpA-(1 to 2)-α-d-Manp-(1 to repeating groups. Since VTh contained 24% GlcA, neither its monosaccharide ratio (GC-MS of alditol acetates) nor methylation re ...
DOI:
10.1016/j.carbpol.2007.09.005

19. A novel branched αβ-glucan isolated from the basidiocarps of the edible mushroom Pleurotus florida

Author:
Iacomini, M.,   et al.   ; Santos-Neves, J.C.; Pereira, M.I.; Carbonero, E.R.; Gracher, A.H.P.; Alquini, G.; Gorin, P.A.J.; Sassaki, G.L.;     Show all 8 Authors
Source:
Carbohydrate polymers 2008 v.73 no.2 pp. 309-314
ISSN:
0144-8617
Subject:
Pleurotus florida; chemical composition; mushrooms; glucans; extraction; extracts; fruiting bodies; chemical structure
Abstract:
... As polysaccharides of the mushroom genus Pleurotus can act as biological response modifiers, we now describe the chemical characterization of a new water-soluble αβ-glucan from the fruiting bodies of the edible mushroom Pleurotus florida. It was obtained via successive aq. extraction, freeze-thawing, precipitation with Fehling solution, and dialysis through a membrane with M(r) 1000 kDa cut-off. I ...
DOI:
10.1016/j.carbpol.2007.11.030

20. An unusual water-soluble β-glucan from the basidiocarp of the fungus Ganoderma resinaceum

Author:
Iacomini, M.,   et al.   ; Amaral, A.E.; Carbonero, E.R.; Simão, R. de C.G.; Kadowaki, M.K.; Sassaki, G.L.; Osaku, C.A.; Gorin, P.A.J.;     Show all 8 Authors
Source:
Carbohydrate polymers 2008 v.72 no.3 pp. 473-478
ISSN:
0144-8617
Subject:
water solubility; chemical composition; nuclear magnetic resonance spectroscopy; methylation; Ganoderma; spectral analysis; molecular weight; beta-glucans; fruiting bodies; chemical structure; fungi
Abstract:
... An unusual water-soluble glucan was isolated from the fruiting bodies of Ganoderma resinaceum following alkaline extraction. Its structure was determined using NMR spectroscopy (13C, 1H, and 1H (obs.), 13C HMQC), methylation analysis, and controlled Smith degradation. It was a highly branched β-d-glucan containing a (1 to 3)-linked β-d-glucopyranosyl main chain, partially substituted at O-6, princ ...
DOI:
10.1016/j.carbpol.2007.09.016

21. Chemical composition of lipopolysaccharides isolated from various endophytic nitrogen-fixing bacteria of the genus Herbaspirillum

Author:
Iacomini, M.,   et al.   ; Serrato, R.V.; Sassaki, G.L.; Cruz, L.M.; Carlson, R.W.; Muszyński, A.; Monteiro, R.A.; Pedrosa, F.O.; Souza, E.M.;     Show all 9 Authors
Source:
Canadian journal of microbiology 2010 v.56 no.4 pp. 342–347
ISSN:
0008-4166
Subject:
Herbaspirillum; nitrogen-fixing bacteria; lipopolysaccharides; carbohydrate composition; cell membranes; endophytes
DOI:
10.1139/W10-011

22. Rheological and structural characteristics of peach tree gum exudate

Author:
Iacomini, M.,   et al.   ; Simas-Tosin, F.F.; Barraza, R.R.; Petkowicz, C.L.O.; Silveira, J.L.M.; Sassaki, G.L.; Santos, E.M.R.; Gorin, P.A.J.;     Show all 8 Authors
Source:
Food hydrocolloids 2010 v.24 no.5 pp. 486-493
ISSN:
0268-005X
Subject:
plant exudates; gel strength; freeze drying; salts; hydrocolloids; viscosity; chemical precipitation; plant extracts; gels; Prunus persica; shear stress; polysaccharides; chemical structure
Abstract:
... The peach tree (Prunus persica), after mechanical injury or infection by microorganisms, produces a copious gum exudate on its trunk and branches. After aqueous extraction, peach gum (cv. ‘chimarrita') yielded a soluble aqueous extract (AE) and a particle-rich hydrogel fraction (RG). After freeze-drying, AE was solubilized in water and added to excess ethanol to give a polysaccharide (POLY) precip ...
DOI:
10.1016/j.foodhyd.2009.12.010