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1. Establishment and functional characterization of a murine primary Sertoli cell line deficient of connexin43

Author:
Rode, Kristina,   et al.   ; Gerber, Jonathan; Hambruch, Nina; Langeheine, Marion; Schnepel, Nadine; Brehm, Ralph;     Show all 6 Authors
Source:
Cell and tissue research 2020 v.381 no.2 pp. 309-326
ISSN:
0302-766X
Subject:
Sertoli cells; Western blotting; assays; cell culture; cell lines; cell proliferation; dynamics; electrical resistance; fluorescent antibody technique; gap junctions; genotype; immunohistochemistry; mice; polymerase chain reaction; research; seminiferous epithelium; spermatogenesis; tight junctions
Abstract:
... The Sertoli cell (SC) specific connexin43 (Cx43) knockout (SCCx43KO) mouse line is ideal to gain insight into the mechanistic gap junction formation in SC and the seminiferous epithelium. A method for developing primary SC cultures from these mice was established, validated and successfully characterized via polymerase chain reaction, immunohistochemistry, immunofluorescence (IF), and Western blot ...
DOI:
10.1007/s00441-020-03203-y

2. Expression of claudin-11 in canine prepubertal testes, and in canine adult testes showing normal spermatogenesis, impaired spermatogenesis, or testicular neoplasia

Author:
Rode, Kristina,   et al.   ; Pörtner, Carolin; Hollenbach, Julia; Thiemeyer, Heike; Beineke, Andreas; Günzel-Apel, Anne-Rose; Brehm, Ralph;     Show all 7 Authors
Source:
Theriogenology 2020 v.148 pp. 122-131
ISSN:
0093-691X
Subject:
Leydig cells; Sertoli cells; Western blotting; adults; antibodies; atrophy; cytoplasm; dogs; fluorescent antibody technique; gene expression regulation; immunohistochemistry; messenger RNA; neoplasm progression; neoplasms; protein content; proteins; puberty; reverse transcriptase polymerase chain reaction; spermatogenesis; staining; tight junctions; transcription (genetics)
Abstract:
... The blood-testis barrier (BTB) consists of different cell-to-cell connections, including tight junction proteins like claudin-11 (CLDN11). For dogs, only limited data is published dealing with these proteins in general. Therefore, their physiological relevance, their postnatal expression, and their distribution pattern in pathological conditions, e.g. in altered spermatogenesis and testicular neop ...
DOI:
10.1016/j.theriogenology.2020.03.001

3. Loss of connexin 43 in Sertoli cells provokes postnatal spermatogonial arrest, reduced germ cell numbers and impaired spermatogenesis

Author:
Rode, Kristina,   et al.   ; Weider, Karola; Damm, Oliver Siegfried; Wistuba, Joachim; Langeheine, Marion; Brehm, Ralph;     Show all 6 Authors
Source:
Reproductive Biology 2018 v.18 no.4 pp. 456-466
ISSN:
1642-431X
Subject:
Sertoli cells; adulthood; adults; apoptosis; connexins; gap junctions; genotype; immunohistochemistry; males; mice; postnatal development; puberty; spermatocytes; spermatogenesis; spermatogonia
Abstract:
... For the reason that adult Sertoli cell specific connexin 43 knockout (SCCx43KO) mice show arrested spermatogenesis at spermatogonial level or Sertoli cell only tubules and significantly reduced germ cell (GC) numbers, the aims of the present study were (1) to characterize the remaining GC population and (2) to elucidate possible mechanisms of their fading. Apoptosis was analyzed in both, KO and wi ...
DOI:
10.1016/j.repbio.2018.08.001

4. Effects of Repeated Testicular Biopsies in Adult Warmblood Stallions and Their Diagnostic Potential

Author:
Rode, Kristina,   et al.   ; Sieme, Harald; Otzen, Henning; Schwennen, Cornelia; Lüpke, Matthias; Richterich, Peter; Schrimpf, Rahel; Distl, Ottmar; Brehm, Ralph;     Show all 9 Authors
Source:
Journal of equine veterinary science 2016 v.38 pp. 33-47
ISSN:
0737-0806
Subject:
Western blotting; adults; adverse effects; biopsy; blood flow; diagnostic techniques; drugs; immunohistochemistry; intracytoplasmic sperm injection; libido; messenger RNA; molecular biology; polymerase chain reaction; proteins; seasonal variation; spermatogenesis; spermatozoa; staining; stallions; surface temperature; testes; thermography; ultrasonography
Abstract:
... Testicular biopsy is still uncommonly used in equine andrological diagnostics although several studies supported that it is a relatively safe procedure. So far, no data exist regarding repeated testicular biopsies in stallions. In the present study, repeated testicular biopsy samples were obtained from four stallions at 4-week intervals alternatingly from both testes over the period of 1 year. The ...
DOI:
10.1016/j.jevs.2016.01.003

5. Identification of vessel degeneration and endometrosis in the equine endometrium, using narrow-band imaging hysteroscopy

Author:
Rode, Kristina,   et al.   ; Otzen, Henning; Sieme, Harald; Oldenhof, Harriëtte; Ertmer, Franziska; Kehr, Anne; Klose, Kristin; Rohn, Karl; Schoon, Heinz-Adolf; Meinecke, Burkhard;     Show all 10 Authors
Source:
Theriogenology 2016 v.86 no.6 pp. 1445-1452
ISSN:
0093-691X
Subject:
biopsy; endometrium; hysteroscopy; image analysis; mares; slaughterhouses; white light
Abstract:
... In this study, endometrosis and angiosclerosis in mares were studied. Endometrosis is a severe, progressive, and irreversible fibrotic condition that affects the endometrium, whereas angiosclerosis refers to thickening of vessel walls due to degenerative changes leading to reduced elasticity of the walls and lower perfusion. Histologic evaluations were performed on biopsies and compared with vascu ...
DOI:
10.1016/j.theriogenology.2016.05.002

6. Epigenetic effects of prenatal estradiol-17β exposure on the reproductive system of pigs

Author:
Rode, Kristina,   et al.   ; Kradolfer, David; Flöter, Veronika L.; Bick, Jochen T.; Fürst, Rainer W.; Brehm, Ralph; Henning, Heiko; Waberski, Dagmar; Bauersachs, Stefan; Ulbrich, Susanne E.;     Show all 10 Authors
Source:
Molecular and Cellular Endocrinology 2016 v.430 pp. 125-137
ISSN:
0303-7207
Subject:
DNA methylation; adults; adverse effects; boars; body weight; endocrine-disrupting chemicals; epigenetics; gene expression; genes; gilts; models; neoplasms; phenotype; piglets; pregnancy; progeny; risk; rodents; sequence analysis; spermatozoa; testes; transcription (genetics)
Abstract:
... There is growing evidence that early life exposure to endocrine disrupting chemicals might increase the risk for certain adult onset diseases, in particular reproductive health problems and hormone dependent cancers. Studies in rodents suggest that perinatal exposure to even low doses of estrogenic substances can cause adverse effects, including epigenetic reprogramming of the prostate and increas ...
DOI:
10.1016/j.mce.2016.04.005

7. Equine endometrial vascular pattern changes during the estrous cycle examined by Narrow Band Imaging hysteroscopy

Author:
Rode, Kristina,   et al.   ; Otzen, Henning; Sieme, Harald; Oldenhof, Harriëtte; Kassens, Ana; Ertmer, Franziska; Müller, Kristin; Klose, Kristin; Rohn, Karl; Schoon, Heinz-Adolf; Meinecke, Burkhard;     Show all 11 Authors
Source:
Animal reproduction science 2016 v.166 pp. 80-89
ISSN:
0378-4320
Subject:
angiogenesis; angiopoietin-2; biopsy; blood; diestrus; endometrium; estrus; hysteroscopy; image analysis; mares; nutrients; ovulation; vascular endothelial growth factor A
Abstract:
... The aim of this study was to evaluate the uterine blood supply and endometrial vessel architecture, during the equine estrous cycle. Narrow Band Imaging (NBI) hysteroscopy was used for evaluating changes in the endometrial vasculature during the estrous cycle [six mares, d 0 (representing the day of ovulation), d 6 and 11 in four locations]. In addition, endometrial biopsy samples were used for im ...
DOI:
10.1016/j.anireprosci.2016.01.006