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- Author:
- Nicolai Bovin, et al. ; Nadezhda Shilova; Diana Maltseva; Svetlana Polyakova; Marina Sablina; Hideaki Niwa; Galina Zakharova; Maria Raygorodskaya; Lyuba Bufeeva; Yury Belyi; Dmitry Hushpulian; Alexander Tonevitsky; Show all 12 Authors
- Source:
- Biochimie 2022 pp. -
- ISSN:
- 0300-9084
- Subject:
- N-acetyllactosamine; cancer therapy; gangliosides; lactose; lectins; mammals; moieties; polysaccharides; protein structure
- Abstract:
- ... Viscumin, a lectin used in anti-cancer therapy, was originally considered as βGal recognizing protein; later, an ability to bind 6′-sialyl N-acetyllactosamine (6′SLN) terminated gangliosides was found. Here we probed viscumin with a printed glycan array (PGA) containing a large number of mammalian sulfated glycans, and found a strong binding to glycans with 6-O-SuGal moiety as lactose, N-acetyllac ...
- DOI:
- 10.1016/j.biochi.2022.08.009
- https://doi.org/10.1016/j.biochi.2022.08.009
- Author:
- Nicolai V. Bovin, et al. ; Roman A. Kunetskiy; Galina V. Pazynina; Igor A. Ivanov; Show all 4 Authors
- Source:
- Carbohydrate research 2020 v.498 pp. 108192
- ISSN:
- 0008-6215
- Subject:
- blood groups; galactose; glycosides; humans; immunotherapy; moieties; research; trisaccharides
- Abstract:
- ... The traditionally used strategy for the synthesis of blood group A and B tetrasaccharides includes 2′-O-fucosylation of lactosamine followed by insertion of an α1-3 linked N-acetylgalactosamine or a galactose moiety. Here, we report the synthesis of 3-aminopropyl glycosides of A (type 2) and B (type 2) tetrasaccharides via an alternative sequence, i.e. α-galactosaminylation (or α-galactosylation) ...
- DOI:
- 10.1016/j.carres.2020.108192
- https://doi.org/10.1016/j.carres.2020.108192
- Author:
- Andrey D. Bovin, et al. ; Irina V. Leppyanen; Olga A. Pavlova; Elena A. Dolgikh; Show all 4 Authors
- Source:
- BIO web of conferences 2020 v.23 pp. 03004
- ISSN:
- 2117-4458
- Subject:
- Pisum sativum; RNA interference; eukaryotic cells; gene expression; genes; legumes; nodulation; symbiosis
- Abstract:
- ... Heterotrimeric G proteins are involved in the regulation of signaling pathways in eukaryotes. Previously, the data about possible participation of heterotrimeric G proteins in the regulation of nodulation in legumes were obtained, however, specific proteins, their composition and role in this process remain poorly understood. In this work searching of the genes encoding the alpha, beta, and gamma ...
- DOI:
- 10.1051/bioconf/20202303004
- https://doi.org/10.1051/bioconf/20202303004
- Author:
- Nicolai Bovin, et al. ; Sudipta Dutta; Kazuhiro Aoki; Kankanit Doungkamchan; Michael Tiemeyer; David J. Miller; Show all 6 Authors
- Source:
- Journal of Biological Chemistry 2019 v.294 no.36 pp. 13445-13463
- ISSN:
- 1083-351X
- Subject:
- cattle; chemistry; immunohistochemistry; insemination; laminin; lectins; longevity; oviducts; polysaccharides; polyspermy; spermatozoa; trisaccharides; viability
- Abstract:
- ... A fraction of sperm deposited at mating or insemination reaches the oviduct isthmus, where sperm are retained and thereby form a reservoir. This reservoir delays capacitation, prevents polyspermy, selects a fertile population of sperm, and, foremost, increases sperm lifespan. The molecular interactions underlying the formation of a sperm reservoir are becoming clearer in mammals. Sperm lectins bin ...
- Handle:
- 10113/7344781
- DOI:
- 10.1074/jbc.ra119.007695
- CHORUS:
- 10.1074/jbc.ra119.007695
- https://doi.org/10.1074/jbc.ra119.007695
- Author:
- Nicolai Bovin, et al. ; Kira Dobrochaeva; Nailya Khasbiullina; Nadezhda Shilova; Nadezhda Antipova; Polina Obukhova; Tatiana Ovchinnikova; Oxana Galanina; Ola Blixt; Horst Kunz; Alexander Filatov; Yuriy Knirel; Jacques LePendu; Sergey Khaidukov; Show all 14 Authors
- Source:
- Molecular immunology 2020 v.120 pp. 74-82
- ISSN:
- 0161-5890
- Subject:
- affinity chromatography; amino acid sequences; amino acids; antibodies; antibody binding sites; enzyme-linked immunosorbent assay; epitopes; flow cytometry; glycopeptides; humans; immunoglobulin M; ligands; microarray technology; mucins; oligosaccharides; polysaccharides; vaccines
- Abstract:
- ... To understand the role of human natural IgM known as antibodies against the carbohydrate epitope Tn, the antibodies were isolated using GalNAcα−Sepharose affinity chromatography, and their specificity was profiled using microarrays (a glycan array printed with oligosaccharides and bacterial polysaccharides, as well as a glycopeptide array), flow cytometry, and inhibition ELISA. The antibodies boun ...
- DOI:
- 10.1016/j.molimm.2020.02.005
- https://doi.org/10.1016/j.molimm.2020.02.005
- Author:
- N.V. Bovin, et al. ; K.L. Dobrochaeva; N.R. Khasbiullina; N.V. Shilova; P.S. Obukhova; Yu.A. Knirel; A.Yu. Nokel; Show all 7 Authors
- Source:
- Molecular immunology 2019 v.106 pp. 63-68
- ISSN:
- 0161-5890
- Subject:
- agarose; antibodies; blood serum; crosslinking; epitopes; humans; immunoglobulins; ligands
- Abstract:
- ... Sepharose matrix without immobilized ligands binds antibodies from human blood serum or immunoglobulin preparations. The eluted antibodies bind bacterial polysaccharides having no structural similarity to agarose (Sepharose is a cross-linked polysaccharide agarose) with a high affinity. It is concluded that the identified antibodies are capable of recognizing spatial rather than linear epitopes of ...
- DOI:
- 10.1016/j.molimm.2018.12.011
- https://doi.org/10.1016/j.molimm.2018.12.011
- Author:
- Nicolai Bovin, et al. ; Petra Sýkorová; Jitka Novotná; Gabriel Demo; Guillaume Pompidor; Eva Dubská; Jan Komárek; Eva Fujdiarová; Josef Houser; Lucia Hároníková; Annabelle Varrot; Nadezhda Shilova; Anne Imberty; Martina Pokorná; Michaela Wimmerová; Show all 15 Authors
- Source:
- International journal of biological macromolecules 2020 v.152 pp. 1113-1124
- ISSN:
- 0141-8130
- Subject:
- Burkholderia pseudomallei; Chromobacterium violaceum; adhesion; bacteria; crystal structure; humans; lectins; polysaccharides; subtropical soils
- Abstract:
- ... Burkholderia pseudomallei and Chromobacterium violaceum are bacteria of tropical and subtropical soil and water that occasionally cause fatal infections in humans and animals. Microbial lectins mediate the adhesion of organisms to host cells, which is the first phase in the development of infection. Here we report the discovery of two novel lectins from the above-mentioned bacteria – BP39L and CV3 ...
- DOI:
- 10.1016/j.ijbiomac.2019.10.200
- https://doi.org/10.1016/j.ijbiomac.2019.10.200
- Author:
- Nicolai Bovin, et al. ; Christopher J. Day; Lauren E. Hartley-Tassell; Kate L. Seib; Joe Tiralongo; Silvana Savino; Vega Masignani; Michael P. Jennings; Show all 8 Authors
- Source:
- Biochemical and biophysical research communications 2019 v.513 no.1 pp. 287-290
- ISSN:
- 0006-291X
- Subject:
- Pseudomonas aeruginosa; blood group antigens; cystic fibrosis; galactose; humans; hyaluronic acid; lectins; patients; secondary infection; surface plasmon resonance; surface proteins; vaccine development; vaccines
- Abstract:
- ... Pseudomonas aeruginosa is an opportunistic pathogen that causes nosocomial infections most commonly in immunocompromised, cystic fibrosis (CF) and burns patients. The pilin and Pseudomonas lectins 1 (PA-IL) and 2 (PA-IIL) are known glycan-binding proteins of P. aeruginosa that are involved in adherence to host cells, particularly CF host airways. Recently, new P. aeruginosa surface proteins were i ...
- DOI:
- 10.1016/j.bbrc.2019.03.092
- https://doi.org/10.1016/j.bbrc.2019.03.092
- Author:
- Nicolai Bovin, et al. ; Daria Tretiakova; Natalia Onishchenko; Ivan Boldyrev; Ilya Mikhalyov; Alexander Tuzikov; Evgeniy Evtushenko; Elena Vodovozova; Show all 8 Authors
- Source:
- Colloids and surfaces 2018 v.166 pp. 45-53
- ISSN:
- 0927-7765
- Subject:
- blood serum; colloids; drug therapy; energy transfer; fluorescence; gangliosides; humans; hydrodynamics; lipid bilayers; lipopeptides; liquids; polyethylene glycol; surfactants; zeta potential
- Abstract:
- ... Previously, we proposed a liposomal formulation of melphalan (Mlph)—a chemotherapeutic alkylating agent—incorporated in a fluid lipid bilayer in the form of dioleoylglyceride ester. In this work, we compared the stabilizing effect of different amphiphiles included in the Mlph-liposomes, such as phosphatidylinositol (PI), ganglioside GM1, a conjugate of N-carboxymethyl-modified oligoglycine with di ...
- DOI:
- 10.1016/j.colsurfb.2018.02.061
- https://doi.org/10.1016/j.colsurfb.2018.02.061
- Author:
- Nicolai V. Bovin, et al. ; Tatiana V. Tyrtysh; Elena Yu Korchagina; Ivan M. Ryzhov; Show all 4 Authors
- Source:
- Carbohydrate research 2017 pp. -
- ISSN:
- 0008-6215
- Subject:
- blood groups; glycosides; hydroxyl radicals; stereochemistry; trisaccharides
- Abstract:
- ... Gram scale synthesis of A (type 2) and B (type 2) tetrasaccharides in the form of 3-aminopropyl glycosides is proposed starting from 3-O-benzoyl-1,6-anhydro-N-acetylglucosamine. Its galactosylation followed by re-protection gave lactosamine derivative with single free 2′OH group in total yield 75%. Standard fucosylation and next run of re-protection in total yield 88% gave a trisaccharide Fuc-Gal- ...
- DOI:
- 10.1016/j.carres.2017.06.014
- https://doi.org/10.1016/j.carres.2017.06.014
- Author:
- Nicolai V. Bovin, et al. ; Rebecca A. Winters; Lauren M. Nettenstrom; Domingo G. Lopez; Kilby L. Willenburg; Ramakrishnan Vishwanath; David J. Miller; Show all 7 Authors
- Source:
- Theriogenology 2018 v.108 pp. 22-28
- ISSN:
- 0093-691X
- Subject:
- Y chromosome; acrosome; boars; cell aggregates; epithelium; mannose; oviducts; polysaccharides; trisaccharides
- Abstract:
- ... This study examined the hypothesis that flow sorting sperm by sex chromosomes affects oviduct cell binding which would influence formation of the sperm reservoir in the oviduct. The sperm-rich fraction from boars (n = 5) was collected, sperm were stained with Hoechst 33342 and sorted. Sperm were sorted based on the presence of either an X or Y chromosome and placed into the following treatments: 1 ...
- DOI:
- 10.1016/j.theriogenology.2017.11.010
- CHORUS:
- 10.1016/j.theriogenology.2017.11.010
- https://doi.org/10.1016/j.theriogenology.2017.11.010
- Author:
- Nicolai Bovin, et al. ; Galina Pazynina; Marina Sablina; Tatiana Ovchinnikova; Tatiana Tyrtysh; Svetlana Tsygankova; Alexander Tuzikov; Kira Dobrochaeva; Nadezhda Shilova; Nailia Khasbiullina; Show all 10 Authors
- Source:
- Carbohydrate research 2017 v.445 pp. 23-31
- ISSN:
- 0008-6215
- Subject:
- antibodies; humans; ligands; microarray technology; polysaccharides
- Abstract:
- ... Our understanding of biological role of glycans O-sulfation remains at the level of beginners due to microheterogeneity, lability and other difficulties of exact structural assignment. Partially, problem of functional investigations, especially determination of glycoepitope specificity of carbohydrate-binding proteins could be solved with the help of synthetic glycans of certain structure. Here we ...
- DOI:
- 10.1016/j.carres.2017.03.015
- https://doi.org/10.1016/j.carres.2017.03.015
- Author:
- Nicolai Bovin, et al. ; Pavel Volynsky; Roman Efremov; Ilya Mikhalev; Kira Dobrochaeva; Alexander Tuzikov; Elena Korchagina; Polina Obukhova; Evgenia Rapoport; Show all 9 Authors
- Source:
- Molecular Immunology 2017 pp. -
- ISSN:
- 0161-5890
- Subject:
- antibodies; blood groups; cholesterol; endothelial cells; enzyme-linked immunosorbent assay; epitopes; erythrocytes; flow cytometry; glycolipids; humans; immunochemistry; lipid bilayers; molecular dynamics; polysaccharides; trisaccharides
- Abstract:
- ... Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration. Previously, we have found natural antibodies capable of binding to trisaccharide Galα1-4Galβ1-4Glc (Pk) practically in all humans. Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) − normal component of erythrocyte and endothelial ...
- DOI:
- 10.1016/j.molimm.2017.06.247
- https://doi.org/10.1016/j.molimm.2017.06.247
- Author:
- Nicolai Bovin, et al. ; Radoslaw Kaczmarek; Maria Duk; Katarzyna Szymczak; Elena Korchagina; Jolanta Tyborowska; Krzysztof Mikolajczyk; Boguslaw Szewczyk; Ewa Jaskiewicz; Marcin Czerwinski; Show all 10 Authors
- Source:
- Biochemical and biophysical research communications 2016 v.470 no.1 pp. 168-174
- ISSN:
- 0006-291X
- Subject:
- amino acids; blood group antigens; blood groups; genes; glycosphingolipids; humans; insects; mutation; open reading frames
- Abstract:
- ... Human Gb3/CD77 synthase (α1,4-galactosyltransferase, Pᵏ synthase), encoded by A4GALT gene, is known for synthesis of Gal(α1-4)Gal moiety in globotriaosylceramide (Gb3Cer, CD77, Pᵏ blood group antigen), a glycosphingolipid of the globo series. Recently, it was shown that c.631C > G mutation in A4GALT, which causes p.Q211E substitution in the open reading frame of the enzyme, broadens the enzyme spe ...
- DOI:
- 10.1016/j.bbrc.2016.01.017
- https://doi.org/10.1016/j.bbrc.2016.01.017
- Author:
- Nicolai V. Bovin, et al. ; Ivan M. Ryzhov; Elena Yu. Korchagina; Inna S. Popova; Tatiana V. Tyrtysh; Alexander S. Paramonov; Show all 6 Authors
- Source:
- Carbohydrate research 2016 v.430 pp. 59-71
- ISSN:
- 0008-6215
- Subject:
- blood groups; glucosamine; glycosides; glycosylation; humans; trisaccharides
- Abstract:
- ... Herein we report the synthesis of 3-aminopropyl glycosides of A (type 2) and B (type 2) tetrasaccharides via [3 + 1] block scheme. Peracetylated trichloroacetimidates of A and B trisaccharides were used as glycosyl donors. The well-known low reactivity of 4-OH group of N-acetyl-d-glucosamine forced us to test four glucosamine derivatives (3-Bz-1,6-anhydro-GlcNAc and 3-trifluoroacetamidopropyl β-gl ...
- DOI:
- 10.1016/j.carres.2016.04.029
- https://doi.org/10.1016/j.carres.2016.04.029
- Author:
- Nicolai Bovin, et al. ; Tatiana Pochechueva; Alexander Chinarev; André Fedier; Francis Jacob; Viola Heinzelmann-Schwarz; Show all 6 Authors
- Source:
- Journal of Immunological Methods 2014 v.412 pp. 42-52
- ISSN:
- 0022-1759
- Subject:
- antibodies; biotin; blood serum; diagnostic techniques; fluorescence; glycosylation; human diseases; humans; immunoassays; immunoglobulin G; immunoglobulin M; microbeads; neoplasms; polyethylene glycol; screening
- Abstract:
- ... Glycan-based suspension array (SGA) is an “in-house” developed multi-target immunoassay, employing commercially available fluorescent microbeads as a solid support for unique chemically synthesized glycopolymers which capture naturally occurring human anti-glycan antibodies. SGA is a sensitive and reliable tool for the high-throughput screening of anti-glycan antibody alterations characteristic fo ...
- DOI:
- 10.1016/j.jim.2014.06.015
- https://doi.org/10.1016/j.jim.2014.06.015
- Author:
- Nicolai V. Bovin, et al. ; Dolores Solís; Anthony P. Davis; Jesús Jiménez-Barbero; Antonio Romero; René Roy; Karel Smetana; Hans-Joachim Gabius; Show all 8 Authors
- Source:
- BBA - General Subjects 2015 v.1850 pp. 186-235
- ISSN:
- 0304-4165
- Subject:
- glycoproteins; lectins; ligands; oligosaccharides; polysaccharides; receptors; rendering; structure-activity relationships; surveys; synthesis
- Abstract:
- ... The most demanding challenge in research on molecular aspects within the flow of biological information is posed by the complex carbohydrates (glycan part of cellular glycoconjugates). How the ‘message’ encoded in carbohydrate ‘letters’ is ‘read’ and ‘translated’ can only be unraveled by interdisciplinary efforts.This review provides a didactic step-by-step survey of the concept of the sugar code ...
- DOI:
- 10.1016/j.bbagen.2014.03.016
- https://doi.org/10.1016/j.bbagen.2014.03.016
- Author:
- Nicolai V. Bovin, et al. ; Ivan M. Ryzhov; Elena Yu. Korchagina; Alexander B. Tuzikov; Inna S. Popova; Tatiana V. Tyrtysh; Galina V. Pazynina; Stephen M. Henry; Show all 8 Authors
- Source:
- Carbohydrate research 2016 v.435 pp. 83-96
- ISSN:
- 0008-6215
- Subject:
- blood groups; chimerism; erythrocytes; glycosides; immunochemistry; oligosaccharides; polysaccharides; serological surveys
- Abstract:
- ... Seven lipophilic constructs containing Lewis (Lea, Leb, Ley) or chimeric Lewis/ABH (ALeb, BLeb, ALey, BLey) glycans were obtained starting from corresponding oligosaccharides in form of 3-aminopropyl glycosides. ALeb and BLeb pentasaccharides were synthesized via [3 + 1] blockwise approach. The constructs (neoglycolipids, or FSLs) were inserted in erythrocyte membrane, and obtained “kodecytes” wer ...
- DOI:
- 10.1016/j.carres.2016.09.016
- https://doi.org/10.1016/j.carres.2016.09.016
19. Pleiotropic effects of amino acid substitutions in H5 hemagglutinin of influenza A escape mutants
- Author:
- Nicolai V. Bovin, et al. ; Nikolai V. Kaverin; Irina A. Rudneva; Tatiana A. Timofeeva; Anna V. Ignatieva; Aleksandr A. Shilov; Natalia A. Ilyushina; Show all 7 Authors
- Source:
- Virus research 2015 v.210 pp. 81-89
- ISSN:
- 0168-1702
- Subject:
- amino acid substitution; heat inactivation; hemagglutinins; influenza; monitoring; mutants; pH; pandemic; phenotype; pleiotropy; prediction; receptors; temperature; thermal stability; viruses
- Abstract:
- ... We believe that the monitoring of pleiotropic effects of the hemagglutinin (HA) mutations found in H5 escape mutants is essential for accurate prediction of mutants with pandemic potential. In the present study, we assessed multiple characteristics of antibody-selected HA mutations. We examined the pH optimum of fusion, HA heat inactivation, affinity to sialyl receptors, and in vitro and in vivo r ...
- DOI:
- 10.1016/j.virusres.2015.07.016
- https://doi.org/10.1016/j.virusres.2015.07.016
- Author:
- Nicolai V. Bovin, et al. ; Natalia A. Ilyushina; Evgeny S. Chernyy; Elena Y. Korchagina; Aleksandra S. Gambaryan; Stephen M. Henry; Show all 6 Authors
- Source:
- Virologica Sinica 2014 v.29 no.4 pp. 199-210
- ISSN:
- 1674-0769
- Subject:
- Influenza A virus; fluorescent labeling; hemagglutinins; lipids; pathogenicity; receptors; sialidase; viral proteins; viruses
- Abstract:
- ... Direct labeling of virus particles is a powerful tool for the visualization of virus-cell interaction events. However, this technique involves the chemical modification of viral proteins that affects viral biological properties. Here we describe an alternative approach of influenza virus labeling that utilizes Function-Spacer-Lipid (FSL) constructs that can be gently inserted into the virus membra ...
- DOI:
- 10.1007/s12250-014-3475-1
- PubMed:
- 25160755
- https://doi.org/10.1007/s12250-014-3475-1
- Author:
- Nicolai Bovin, et al. ; Elena Korchagina; Alexander Tuzikov; Andrey Formanovsky; Inna Popova; Stephen Henry; Show all 6 Authors
- Source:
- Carbohydrate research 2012 v.356 no. pp. 238-246
- ISSN:
- 0008-6215
- Subject:
- antibodies; cell membranes; disaccharides; hydrophilicity; polysaccharides; toxins; virion
- Abstract:
- ... Synthetic glycolipid-like constructs dispersible in biological media and capable of incorporating into cell membranes have the ability to create novel artificial glyco-landscapes on living cells. Using a variety of different glycans ranging from disaccharides to polysaccharides, together with different lengths and high hydrophilicity spacers, we created a series of synthetic glycolipid-like constr ...
- DOI:
- 10.1016/j.carres.2012.03.044
- https://doi.org/10.1016/j.carres.2012.03.044
- Author:
- Nicolai V. Bovin, et al. ; Ivan M. Ryzhov; Elena Yu Korchagina; Inna S. Popova; Show all 4 Authors
- Source:
- Carbohydrate research 2012 v.351 no. pp. 17-25
- ISSN:
- 0008-6215
- Subject:
- blood groups; glycosides; humans; trisaccharides
- Abstract:
- ... Blood group A tetrasaccharides of different types have the same terminal trisaccharide fragment that allows using a block scheme in their synthesis. 3-Aminopropyl glycosides of tetrasaccharides GalNAcα1-3(Fucα1-2)Galβ1-3GlcNAcβ (A type 1), GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcα (A type 3), and GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcβ (A type 4) were synthesised using acetylated Galα1-3(Fucα1-2)Gal trichloroac ...
- DOI:
- 10.1016/j.carres.2011.12.013
- https://doi.org/10.1016/j.carres.2011.12.013
- Author:
- Nicolai V. Bovin, et al. ; Tanja Larsen; Yayoi Yoshimura; Bjørn G.R. Voldborg; Giuseppe Cazzamali; Ulrika Westerlind; Monica M. Palcic; Jørgen J. Leisner; Show all 8 Authors
- Source:
- FEBS letters 2014 v.588 no.5 pp. 746-751
- ISSN:
- 0014-5793
- Subject:
- blood serum; chitin; chitinase; fungi; genetic disorders; glycolipids; glycoproteins; glycosylation; humans; pathogenesis
- Abstract:
- ... Humans do not synthesize chitin, yet they produce a number of active and inactive chitinases. One of the active enzymes is chitotriosidase whose serum levels are elevated in a number of diseases such as Gaucher’s disease and upon fungal infection. Since the biological role of chitotriosidase in disease pathogenesis is not understood we screened a panel of mammalian GlcNAc-containing glycoconjugate ...
- DOI:
- 10.1016/j.febslet.2013.12.035
- https://doi.org/10.1016/j.febslet.2013.12.035
- Author:
- Nicolai V. Bovin, et al. ; Gabriel García Caballero; Andrea Flores-Ibarra; Malwina Michalak; Nailya Khasbiullina; Sabine André; Joachim C. Manning; Sabine Vértesy; Federico M. Ruiz; Herbert Kaltner; Jürgen Kopitz; Antonio Romero; Hans-Joachim Gabius; Show all 13 Authors
- Source:
- Biochimica et biophysica acta 2016 v.1860 no.10 pp. 2285-2297
- ISSN:
- 0304-4165
- Subject:
- X-radiation; binding capacity; cell physiology; chickens; crystal proteins; crystal structure; crystallography; filtration; gel electrophoresis; genes; germplasm conservation; hemagglutination; lactose; lectins; mass spectrometry; microarray technology; models; monitoring; sequence alignment; ultracentrifugation
- Abstract:
- ... Endogenous lectins are multifunctional effectors in cell physiology. Adding the sixth member of the galectin family in chicken, a model organism for systematic profiling of these adhesion/growth-regulatory proteins, is a step toward comprehensive network monitoring.Database mining and computational data processing are applied for gene detection, chromosomal location and sequence alignments. Clonin ...
- DOI:
- 10.1016/j.bbagen.2016.06.001
- PubMed:
- 27268118
- PubMed Central:
- PMC7127388
- https://doi.org/10.1016/j.bbagen.2016.06.001
- Author:
- Nicolai Bovin, et al. ; Polina Obukhova; Nadezhda Shilova; Evgenia Rapoport; Inna Popova; Maksim Navakouski; Carlo Unverzagt; Marko Vuskovic; Margaret Huflejt; Show all 9 Authors
- Source:
- Biochimica et biophysica acta 2012 v.1820 no.9 pp. 1373-1382
- ISSN:
- 0304-4165
- Subject:
- autoantibodies; blood serum; carbohydrate binding; chromatography; epitope mapping; epitopes; glycolipids; humans; immunoglobulins; polysaccharides
- Abstract:
- ... BACKGROUND: Profiling of donor's antibodies using glycan arrays demonstrated presence of antibodies capable of binding to >100 mammalian glycans or their fragments. For example, relatively high binding to Galα1-4Galβ1-4GlcNAc (P₁), Galα1-4Galβ1-4Glc (Pᵏ), Galβ1-3GlcNAc (Leᶜ), 4-O-SuGalβ1-4GlcNAc, and GalNAcα1-3GalNAc (Fs) was found in all tested individuals. Affinity isolation using hapten-specifi ...
- DOI:
- 10.1016/j.bbagen.2012.02.005
- https://doi.org/10.1016/j.bbagen.2012.02.005
- Author:
- Nicolai V. Bovin, et al. ; Natalia R. Kuznetsova; Chantal Sevrin; David Lespineux; Elena L. Vodovozova; Tamás Mészáros; Janos Szebeni; Christian Grandfils; Show all 8 Authors
- Source:
- Journal of controlled release 2012 v.160 no.2 pp. 394-400
- ISSN:
- 0168-3659
- Subject:
- adverse effects; coagulation; complement; compliance; drug delivery systems; erythrocytes; esters; hemolysis; humans; methotrexate; phosphatidylcholines; zeta potential
- Abstract:
- ... A panel of in vitro tests intended for evaluation of the nano-sized drug delivery systems' compliance with human blood was applied to liposomal formulations of anticancer lipophilic prodrugs incorporated into the lipid bilayer. Liposomes on the basis of natural phosphatidylcholine (PC) and phosphatidylinositol (PI), 8:1 (mol) were loaded with 10mol% of either methotrexate or melphalan 1,2-dioleoyl ...
- DOI:
- 10.1016/j.jconrel.2011.12.010
- https://doi.org/10.1016/j.jconrel.2011.12.010
- Author:
- Nicolai V. Bovin, et al. ; Gabriel García Caballero; Herbert Kaltner; Malwina Michalak; Nadezhda Shilova; Michelle Yegres; Sabine André; Anna-Kristin Ludwig; Joachim C. Manning; Sebastian Schmidt; Martina Schnölzer; Dietmar Reusch; Jürgen Kopitz; Hans-Joachim Gabius; Show all 14 Authors
- Source:
- Biochimie 2016 v.128-129 pp. 34-47
- ISSN:
- 0300-9084
- Subject:
- Western blotting; adults; agglutinins; chickens; genes; humans; immunohistochemistry; lactose; mass spectrometry; microarray technology; peptides; rats; reverse transcriptase polymerase chain reaction
- Abstract:
- ... Occurrence of the adhesion/growth-regulatory galectins as family sets the challenge to achieve a complete network analysis. Along this route taken for a well-suited model organism (chicken), we fill the remaining gap to characterize its seventh member known from rat as galectin-related inter-fiber protein (GRIFIN) in the lens. Its single-copy gene is common to vertebrates, with one or more deviati ...
- DOI:
- 10.1016/j.biochi.2016.06.001
- https://doi.org/10.1016/j.biochi.2016.06.001
- Author:
- Nicolai Bovin, et al. ; Irina Rudneva; Anna Ignatieva; Tatiana Timofeeva; Aleksandr Shilov; Alla Kushch; Olga Masalova; Regina Klimova; Larisa Mochalova; Nikolai Kaverin; Show all 10 Authors
- Source:
- Virus research 2012 v.166 no.1-2 pp. 61-67
- ISSN:
- 0168-1702
- Subject:
- Orthomyxoviridae; amino acids; antigenic variation; epitopes; genes; hemagglutinins; influenza; molecular weight; monoclonal antibodies; mutants; neutralization; pandemic; viruses
- Abstract:
- ... A panel of 6 neutralizing monoclonal antibodies (MAbs) raised against A/Moscow/IIV01/2009 (H1N1) virus isolated during the 2009 pandemic was used for the selection of 26 escape mutants. The mutants were characterized in immune cross-reactions with the panel of MAbs. The sequencing of the mutant HA genes revealed 5 amino acid positions recognized by monoclonal antibodies: 129, 156, 158, 159, and 19 ...
- DOI:
- 10.1016/j.virusres.2012.03.003
- https://doi.org/10.1016/j.virusres.2012.03.003
29. Chemical synthesis of 6⁽GlcNAc⁾- and 6⁽Gal⁾-O-sulfated SiaLeX tetrasaccharides in spacer-armed form
- Author:
- Bovin, Nicolai, et al. ; Pazynina, Galina; Sablina, Marina; Mayzel, Maxim; Nasonov, Vitaly; Tuzikov, Alexander; Show all 6 Authors
- Source:
- Glycobiology 2009 v.19 no.10 pp. 1078-1081
- ISSN:
- 0959-6658
- Subject:
- antigens; carbohydrates; ligands; sulfates; synthesis
- Abstract:
- ... Practical synthesis of tetrasaccharide sulfates, 6⁽GlcNAc⁾-O-Su-SiaLeX-OCH₂CH₂CH₂NH₂ and 6⁽Gal⁾-O-Su-SiaLeX-OCH₂CH₂CH₂NH₂ (SuFormula SO₃H), selectin ligands, and leu- kocyte trafficking agents is presented. Both sulfates were synthesized starting from the same precursor, protected SiaLex, by the conventional procedures of carbohydrate chemistry. The sulfated SiaLex derivative was modified at the s ...
- DOI:
- 10.1093/glycob/cwp093
- PubMed:
- 19584101
- PubMed Central:
- PMC2736044
- https://doi.org/10.1093/glycob/cwp093
- Author:
- Nicolai V. Bovin, et al. ; Elizabeth M. Hadac; Mark J. Federspiel; Evgeny Chernyy; Alexander Tuzikov; Elena Korchagina; Stephen Russell; Stephen M. Henry; Show all 8 Authors
- Source:
- Journal of virological methods 2011 v.176 no.1-2 pp. 78-84
- ISSN:
- 0166-0934
- Subject:
- image analysis; liver; virion; radiolabeling; Orthomyxoviridae; Measles morbillivirus; blood flow; mice; pathogenicity; rapid methods; spleen; tyrosine; viruses; Vesiculovirus; fluorescein; half life
- Abstract:
- ... Tools that can aid in vitro and in vivo imaging and also noninvasively determine half-life and biodistribution are required to advance clinical developments. A Function-Spacer-Lipid construct (FSL) incorporating fluorescein (FSL-FLRO4) was used to label vesicular stomatitis virus (VSV), measles virus MV–NIS (MV) and influenza virus (H1N1). The ability of FSL constructs to label these virions was e ...
- DOI:
- 10.1016/j.jviromet.2011.06.005
- https://doi.org/10.1016/j.jviromet.2011.06.005
- Author:
- Bovin Nicolai, et al. ; Pazynina Galina; Nasonov Vitaly; Belyanchikov Ivan; Brossmer Reinchard; Maisel Maxim; Tuzikov Alexander; Russian Foundation for Basic Research; Show all 8 Authors
- Source:
- International journal of carbohydrate chemistry 2010 v.2010 pp. -
- ISSN:
- 1687-935X
- Subject:
- carbohydrate binding; disaccharides; glycosylation; neuraminic acid; proteins; silver
- Abstract:
- ... Earlier we reported a convenient and efficient method of preparing α2-6 sialooligosaccharides in conditions of Koenigs-Knorr reaction. The use of Ag 2 CO 3 allowed carrying out α2-6 sialylation of galacto-4,6-diol of mono- and disaccharides with chloride of acetylated N -acetylneuraminic acid methyl ester as glycosyl donor. In this study we applied this approach to other derivatives of neuraminic ...
- DOI:
- 10.1155/2010/594247
- https://doi.org/10.1155/2010/594247
- Author:
- N. Bovin, et al. ; L. Mochalova; V. Kurova; Y. Shtyrya; E. Korchagina; A. Gambaryan; I. Belyanchikov; Show all 7 Authors
- Source:
- Archives of virology 2007 v.152 no.11 pp. 2047-2057
- ISSN:
- 0304-8608
- Subject:
- Influenza A virus; ducks; influenza; isomers; sialidase; swine; trisaccharides; vertebrate viruses
- Abstract:
- ... A fluorescent neuraminidase (NA) assay has been developed; 20 samples in five replicates could be analyzed at the same time, allowing us to study the kinetics of the enzyme-substrate interaction. The specificities of six influenza H1N1 virus NAs for BODIPY-labeled 3′SiaLac, 3′SiaLacNAc, SiaLeᶜ, SiaLeᵃ, 6′SiaLac, and 6′SiaLacNAc were evaluated. The duck virus NA hydrolyzed 6′SiaLac and 6′SiaLacNAc ...
- DOI:
- 10.1007/s00705-007-1024-z
- PubMed:
- 17680329
- https://doi.org/10.1007/s00705-007-1024-z
- Author:
- Bovin Nicolai V., et al. ; Zhang Fuming; Walcott Benjamin; Zhou Dongwen; Gustchina Alla; Lasanajak Yi; Smith David F.; Ferreira Rodrigo S.; Correia Maria Tereza S.; Paiva Patrícia M. G.; Wlodawer Alexander; Oliva Maria L. V.; Linhardt Robert J.; Show all 13 Authors
- Source:
- Biochemistry 2013 v.52 no.12 pp. 2148-2156
- ISSN:
- 1520-4995
- Subject:
- Crateva tapia; analgesics; bark; binding capacity; blood coagulation; chondroitin sulfate; coagulation; disaccharides; heparan sulfate; heparin; insecticidal properties; kinetics; lectins; proteinase inhibitors; sulfates; surface plasmon resonance; thrombin
- Abstract:
- ... CrataBL, a protein isolated from Crataeva tapia bark, which is both a serine protease inhibitor and a lectin, has been previously shown to exhibit a number of interesting biological properties, including anti-inflammatory, analgesic, antitumor, and insecticidal activities. Using a glycan array, we have now shown that only sulfated carbohydrates are effectively bound by CrataBL. Because this protei ...
- DOI:
- 10.1021/bi400077b
- PubMed:
- 23448527
- PubMed Central:
- PMC3855636
- https://doi.org/10.1021/bi400077b
- Author:
- Nicolai Bovin, et al. ; Diana M.E. Otto; Maria A. Campanero-Rhodes; Rositsa Karamanska; Andrew K. Powell; Jeremy E. Turnbull; Robert A. Field; Jonathan Blackburn; Ten Feizi; Paul R. Crocker; Show all 10 Authors
- Source:
- Analytical biochemistry 2011 v.411 no.2 pp. 261-270
- ISSN:
- 0003-2697
- Subject:
- adhesion; green fluorescent protein; lectins; polysaccharides; protein synthesis; protein-protein interactions; proteinases; screening; surface plasmon resonance; surface proteins
- Abstract:
- ... Here we describe a versatile high-throughput expression system that permits genome-wide screening of type 1 membrane and secreted proteins for interactions with glycans and proteins using both cell-expressed and soluble forms of the expressed proteins. Based on Gateway cloning methodology, we have engineered a destination vector that directs expression of enhanced green fluorescent protein (EGFP)- ...
- DOI:
- 10.1016/j.ab.2010.12.036
- PubMed:
- 21211507
- PubMed Central:
- PMC3740237
- https://doi.org/10.1016/j.ab.2010.12.036
- Author:
- Bovin, Nicolai, et al. ; Turcot-Dubois, Anne-Laure; Le Moullac-Vaidye, Béatrice; Despiau, Stéphanie; Roubinet, Francis; Le Pendu, Jacques; Blancher, Antoine; Show all 7 Authors
- Source:
- Glycobiology 2007 v.17 no.5 pp. 516-528
- ISSN:
- 0959-6658
- Subject:
- Danio rerio; Takifugu; alleles; amino acid sequences; amphibians; cations; chickens; cows; dogs; epitopes; fish; galactose; glycolipids; glycosyltransferases; humans; mice; models; phylogeny; proteins; rats; sequence homology
- Abstract:
- ... Functional glycosyltransferase 6 (GT6) family members catalyze the transfer of galactose or N-acetylgalactosamine in α1,3 linkage to various substrates and synthesize structures related to the A and B histo-blood group antigens, the Forssman antigen, αGal epitope, and iGb3 glycolipid. In rat, mouse, dog, and cow genomes, we have identified three new mammalian genes (GT6m5, GT6m6, and GT6m7) encodi ...
- DOI:
- 10.1093/glycob/cwm016
- https://doi.org/10.1093/glycob/cwm016
- Author:
- Bovin, Nicolai V., et al. ; Rapoport, Eugenia M.; André, Sabine; Kurmyshkina, Olga V.; Pochechueva, Tatiana V.; Severov, Vyacheslav V.; Pazynina, Galina V.; Gabius, Hans -J.; Show all 8 Authors
- Source:
- Glycobiology 2008 v.18 no.4 pp. 315-324
- ISSN:
- 0959-6658
- Subject:
- case studies; cell adhesion; disease course; galectins; ligands; oligosaccharides; polysaccharides
- Abstract:
- ... The involvement of galectins as pleiotropic regulators of cell adhesion and growth in disease progression explains the interest to define their ligand-binding properties. Toward this end, it is desirable to approach in vivo conditions to attain medical relevance. In order to simulate physiological conditions with cell surface glycans as recognition sites and galectins as mediators of intercellular ...
- DOI:
- 10.1093/glycob/cwn009
- https://doi.org/10.1093/glycob/cwn009
- Author:
- Nicolai V. Bovin, et al. ; Zsuzsanna Pál; Péter Antal; Sanjeev Kumar Srivastava; Gábor Hullám; Ágnes F. Semsei; János Gál; Mihály Svébis; Györgyi Soós; Csaba Szalai; Sabine André; Elena Gordeeva; György Nagy; Herbert Kaltner; Mária Judit Molnár; András Falus; Hans-Joachim Gabius; Edit Irén Buzás; Show all 18 Authors
- Source:
- Biochimica et biophysica acta 2012 v.1820 no.10 pp. 1512-1518
- ISSN:
- 0304-4165
- Subject:
- T-lymphocytes; Whites; antibodies; apoptosis; autoimmunity; carbohydrate binding; genes; genotype; lectins; mutants; patients; pleiotropy; rheumatoid arthritis; risk assessment; single nucleotide polymorphism; synovial fluid; thymocytes
- Abstract:
- ... BACKGROUND: Galectins are potent immune regulators, with galectin-8 acting as a pro-apoptotic effector on synovial fluid cells and thymocytes and stimulator on T-cells. To set a proof-of-principle example for risk assessment in autoimmunity, and for a mutation affecting physiological galectin sensor functions, a polymorphism in the coding region of the galectin-8 gene (rs2737713; F19Y) was studied ...
- DOI:
- 10.1016/j.bbagen.2012.05.015
- https://doi.org/10.1016/j.bbagen.2012.05.015
- Author:
- Bovin, Nicolai, et al. ; Townson, Kate; Greenshields, Kay N.; Veitch, Jean; Nicholl, Dawn; Eckhardt, Matthias; Galanina, Oxana; Samain, Eric; Antoine, Tatiana; Bundle, David; Zhang, Ping; Ling, Chang Chun; Willison, Hugh J.; Show all 13 Authors
- Source:
- Glycobiology 2007 v.17 no.11 pp. 1156-1166
- ISSN:
- 0959-6658
- Subject:
- B-lymphocytes; antibodies; antimicrobial properties; binding properties; encapsulation; epitopes; gangliosides; humans; immunomodulation; lectins; ligands; mice; myelin sheath; nerve tissue; plasma membrane; polyacrylamide; polysaccharides
- Abstract:
- ... Antiganglioside antibodies form an important component of the innate and adaptive B cell repertoire, where they provide antimicrobial activity through binding encapsulated bacterial glycans. In an aberrant role, they target peripheral nerve gangliosides to induce autoimmune nerve injury. An important characteristic of antiganglioside antibodies is their ability to selectively recognize highly defi ...
- DOI:
- 10.1093/glycob/cwm095
- https://doi.org/10.1093/glycob/cwm095
- Author:
- Bovin N. V., et al. ; Yamnikova S. S.; Gambaryan A. S.; Tuzikov A. B.; Matrosovich M. N.; Fedyakina I. T.; Grinev A. A.; Blinov V. M.; Lvov D. K.; Suarez D. L.; Swayne D. E.; Show all 11 Authors
- Source:
- Avian diseases 2003 v.47 no.s3 pp. 1164-1168
- ISSN:
- 0005-2086
- Subject:
- Laridae; Influenza A virus; receptors; amino acid sequences; hemagglutinins; binding sites; Anatidae; glycoproteins
- Abstract:
- ... A comparative study of the hemagglutinin (HA) receptor binding site (RBS) of a number of H13 influenza viruses isolated from Laridae family of birds (gulls) and other influenza viruses obtained from the Anatidae family (ducks) was conducted. The affinity of all viruses to alpha N-acetylneuraminic acid (Neu5Acαα), 3′′sialyllactose (3′′SL), and sialylglycopolymers bearing 3′′-sialyl(N-acetyllactosam ...
- Handle:
- 10113/48339
- DOI:
- 10.1637/0005-2086-47.s3.1164
- https://doi.org/10.1637/0005-2086-47.s3.1164