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amino acid sequences; binding sites; mitogen-activated protein kinase; mitogen-activated protein kinase kinase; mutants; phosphorylation; proteins; sequence homology; signal transduction; tyrosine; viruses
Abstract:
... Binding of Src family kinases to membrane-associated polyoma virus middle T-antigen (PyMT) can result in the phosphorylation of PyMT tyrosine 250, which serves as a docking site for the binding of Shc and subsequent activation of the Raf-MEK-ERK (MAP) kinase cascade. In a screen for PyMT variants that could not activate the ARF tumor suppressor, we isolated a cytoplasmic nontransforming mutant (MT ...
traditional medicine; seeds; ethnobotany; sheep; Chenopodium album; medicinal plants; plant extracts; veterinary medicine; Caesalpinia; Haemonchus contortus; animal parasitic nematodes; anthelmintics; chemical constituents of plants; medicinal properties; herbal medicines; Pakistan
Abstract:
... Aim of the study: The present study was carried out to determine the anthelmintic activity of Caesalpinia crista (L.) (Fabaceae) seed kernel and Chenopodium album (L.) (Chenopodiaceae) whole plant in order to justify their traditional use in veterinary medicine. Materials and methods: In vitro anthelmintic activity of crude aqueous methanolic extract (AME) of both the plants was determined using m ...
Erythrina crista-galli, etc ; Frankliniella schultzei; Malvaviscus arboreus; bait traps; copulation; crossing; females; flowers; host plants; insect behavior; males; progeny; sex pheromones; sex ratio; Show all 14 Subjects
Abstract:
... The thrips taxon, Frankliniella schultzei Trybom, could possibly comprise a complex of unrecognised cryptic species. We therefore made a range of reproductive behavioral observations on F. schultzei individuals from different host-associated populations to investigate their species status. We first described the mating behavior of F. schultzei taken from flowers of a primary host species, Malvavis ...
... The amyloid β-peptide (Aβ) has been suggested to exert its toxicity intracellularly. Mitochondrial functions can be negatively affected by Aβ and accumulation of Aβ has been detected in mitochondria. Because Aβ is not likely to be produced locally in mitochondria, we decided to investigate the mechanisms for mitochondrial Aβ uptake. Our results from rat mitochondria show that Aβ is transported int ...
Brawley, Crista M., et al. ; Nagy, Stanislav; Ricca, Benjamin L.; Norstrom, Melanie F.; Courson, David S.; Smithback, Philip A.; Rock, Ronald S.; Show all 7 Authors
... Eukaryotic cells organize their contents through trafficking along cytoskeletal filaments. The leading edge of a typical metazoan cytoskeleton consists of a dense and complex arrangement of cortical actin. A dendritic mesh is found across the broad lamellopodium, with long parallel bundles at microspikes and filopodia. It is currently unclear whether and how myosin motors identify the few actin fi ...
... Adult stem cells often reside in local microenvironments, or niches. Although niches can contain multiple types of stem cells, the coordinate regulation of stem cell behavior is poorly understood. In the Drosophila testis, Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling is directly required for maintenance of the resident germline and somatic stem cells. We found ...
de Farias, Vanessa; Maranho, Leila Teresinha; de Vasconcelos, Eliane Carvalho; da Silva Carvalho Filho, Marco Aurélio; Lacerda, Luiz Gustavo; Azevedo, Jayme Augusto Menegassi; Pandey, Ashok; Soccol, Carlos Ricardo
Erythrina crista-galli, etc ; polluted soils; petroleum; phytoremediation; biodegradation; seed germination; greenhouse experimentation; gas chromatography; mortality; roots; Brazil; Show all 11 Subjects
Abstract:
... This work aimed at investigating both the tolerance and the phytodegradation potential of Erythrina crista-galli L. in petroleum-contaminated soil. It consisted in analyzing E. crista-galli germination, surviving, growth, and development when cultivated at different contaminant concentrations and pollutant degradation rates. This specimen was selected because it presented a special behavior among ...
... Eukaryotic cells have a self-organizing cytoskeleton where motors transport cargoes along cytoskeletal tracks. To understand the sorting process, we developed a system to observe single-molecule motility in a cellular context. We followed myosin classes V, VI, and X on triton-extracted actin cytoskeletons from Drosophila S2, mammalian COS-7, and mammalian U2OS cells. We find that these cells vary ...
... We have developed and tested a robust delivery method for the transport of proteins to the cytoplasm of mammalian cells without compromising the integrity of the cell membrane. This receptor-mediated delivery (RMD) technology utilizes a variant of substance P (SP), a neuropeptide that is rapidly internalized upon interaction with the neurokinin-1 receptor (NK1R). Cargos in the form of synthetic an ...
... Quantitative Structure-Activity Relationship (QSAR) study have been conducted to antimalarial active compounds of cassane-type diterpenes isolated from seed kernels of Bagore (Caesalpinia crista Linn.), using the atomic net charges data resulted from AM1 method of semi-empirical calculations. The aim of this research is to determine the mathematic equation model of quantitative structure-activity ...
... In the present study, Apis mellifera venom (AmV) was tested for its ability to cause ultrastructural changes in mitochondria of rat adrenal cortex in vivo. In order to achieve this goal, different AmV treatments were performed and the effects were quantified on transmission electron micrographs. In a first experimental group, AmV injected for 30 days in low daily doses (700μg/kg) generated importa ...
... This study showed that greige cotton nonwoven fabric can effectively be flame retardant by applying the phosphorus of diammonium phosphate (DAP) as low as 0.8wt% with the addition of urea. At such a low content of phosphorus, the char length and limiting oxygen index (LOI) were continuously decreased and increased, respectively, as the concentration of urea increased. The effect of urea additive o ...
... I examined the effects of growth temperature and winter duration on the leaf phenology of Fauria crista-galli plants, which have an indeterminate growth habit. After a 220-day chilling treatment, the leaf expansion and green periods of plants maintained at 25/20°C were much longer than those of plants maintained at 15/10°C and of plants at the natural habitat obtained in a previous study. The resu ...
... Greenhouse experiments were conducted to assess the effects of supplemental calcium in salinised soil on the response of germination and seedling growth of Caesalpinia crista, L. (Fabaceae). NaCl and CaSOâ·2HâO were added to the soil and 0:0, !:0, 1:0.25, 1:0.50, 1:0.75, 1:1, 1:1.25, and 1:1.50 Na/Ca ratios were maintained. Salinity significantly retarded the seed germination and seedling gro ...
... Several strains of endophytic bacteria and yeast were isolated from Daucus carota, Erythrina crista-galli, Curcubita maxima and Fortunella margarita, and tested for the reduction of two model α-alkyl-β-ketoesters. The yeast strains resulted better biocatalysts for the reduction of the tested substrates with the exception of Enterobacter agglomerans C8 strain, isolated from Erythrina crista-galli, ...
... Drosophila quinaria is polymorphic for infection with Wolbachia, a maternally transmitted endosymbiont. Wolbachia-infected individuals carry mtDNA that is only distantly related to the mtDNA of uninfected individuals, and the clade encompassing all mtDNA haplotypes within D. quinaria also includes the mtDNA of several other species of Drosophila. Nuclear gene variation reveals no difference betwee ...
... We present here a characterization of Erythrina crista-galli L. (syn: Erythrina lamifolia Jacq.) seedlings, obtained from a plant from the Botanical Garden of Pisa University. This plant produces seeds that, during germination, have shown two different seedling phenotypes: normal (NT, 75%) and “albino” types (AT, 25%). Albino seedlings survive only 8–9 weeks and their growth is dramatically reduce ...
Madison Crista A., et al. ; Yoshioka-Tarver Megumi; Condon Brian D.; Santiago Cintrón Michael; Chang SeChin; Easson Michael W.; Fortier Chanel A.; Bland John M.; Nguyen Thach-Mien D.; Show all 9 Authors
Fourier transform infrared spectroscopy; calorimeters; carbon; chemical bonding; cotton; cotton fabric; engineering; flame retardants; flammability; mass spectrometry; nuclear magnetic resonance spectroscopy; oxygen; phosphorus; stable isotopes; thermogravimetry
Abstract:
... In this paper, we studied the synthesis, characterization, and flammability of the water-soluble, nonhalogenated organophosphorus flame retardant (FR) dimethyl-[1,3,5-(3,5-triacryloylhexahydro)triazinyl]-3-oxopropylphosphonate for application to cotton fabrics by nonpolymeric network. The FR was synthesized in a one-step reaction from dimethyl phosphite and 1,3,5-triacryloylhexahydro-1,3,5-triazin ...
stakeholders; environmental education; interviews; models; social networks; landowners; researchers; forests; Maine
Abstract:
... To better engage Maine's family forest landowners our study used social network analysis: a computational social science method for identifying stakeholders, evaluating models of engagement, and targeting areas for enhanced partnerships. Interviews with researchers associated with a research center were conducted to identify how social network analysis could improve knowledge transfer in the resea ...
Crista A. Madison, et al. ; Megumi Yoshioka-Tarver; Brian D. Condon; Michael W. Easson; Sunghyun Nam; Chanel A. Fortier; Bruce F. Ingber; Show all 7 Authors
... The enhanced flame resistance properties of a covalently-bonded flame retardant were observed on fabrics treated with a reactive cyanuric chloride linked compound. The thermal behavior of durable and non-durable finished fabrics were characterized by thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), and flame test. The compound effectively served as a semi-durable f ...
... Mitochondria amplify caspase-dependent apoptosis by releasing proapoptotic proteins, especially cytochrome c. This process is accompanied by mitochondrial cristae remodeling. Our studies demonstrated that mitofilin, a mitochondrial inner membrane protein, acted as a cristae controller to regulate cytochrome c release during apoptosis. Knockdown of mitofilin in HeLa cells with RNAi led to fragmenta ...
... Polar Cartilages and Formation of Crista Sellaris in Grass Snake, Natrix natrix (Ophidia, Colubridae), Chondrocranium at the Early Stages of Embryogenesis. Sheverdyukova H. V.- The initial developmental stages of grass snake’s, Natrix natrix Linnaeus, 1758 chondrocranium are described. Three paired structures form the floor of N. natrix’s neurocranium: cranial trabeculae, polar cartilages, and par ...
Ralf M. Zerbes; Maria Bohnert; David A. Stroud; Karina von der Malsburg; Anita Kram; Silke Oeljeklaus; Bettina Warscheid; Thomas Becker; Nils Wiedemann; Marten Veenhuis; Ida J. van der Klei; Nikolaus Pfanner; Martin van der Laan
... The mitochondrial inner membrane contains a large protein complex crucial for membrane architecture, the mitochondrial inner membrane organizing system (MINOS). MINOS is required for keeping cristae membranes attached to the inner boundary membrane via crista junctions and interacts with protein complexes of the mitochondrial outer membrane. To study if outer membrane interactions and maintenance ...
... We used electron cryotomography of mitochondrial membranes from wild-type and mutant Saccharomyces cerevisiae to investigate the structure and organization of ATP synthase dimers in situ. Subtomogram averaging of the dimers to 3.7 nm resolution revealed a V-shaped structure of twofold symmetry, with an angle of 86° between monomers. The central and peripheral stalks are well resolved. The monomers ...
... Aging is one of the most fundamental, yet least understood biological processes that affect all forms of eukaryotic life. Mitochondria are intimately involved in aging, but the underlying molecular mechanisms are largely unknown. Electron cryotomography of whole mitochondria from the aging model organism Podospora anserina revealed profound age-dependent changes in membrane architecture. With incr ...
Straub Crista L., et al. ; Leahy Jessica E.; Reeves Erika Gorczyca; Bell Kathleen P.; Wilson Jeremy S.; National Science Foundation; University of Maine; Show all 7 Authors
analysis of variance; environmental factors; environmental impact; forests; humans; issues and policy; landowners; logging; models; pests; social change; tree mortality; Maine
Abstract:
... Small-scale forests are an excellent example of coupled social-ecological systems, which involve human and biophysical subsystems with complex two-way feedback interactions. The multifaceted nature of landowner decisions drives a significant need to better understand decision-making processes, reactions to policy, and combined impacts on ecosystems in a comprehensive manner. Small-scale forests re ...
... Mammalian cells typically contain thousands of copies of mitochondrial DNA assembled into hundreds of nucleoids. Here we analyzed the dynamic features of nucleoids in terms of mitochondrial membrane dynamics involving balanced fusion and fission. In mitochondrial fission GTPase dynamin-related protein (Drp1)-deficient cells, nucleoids were enlarged by their clustering within hyperfused mitochondri ...
... The present study aimed to analyse the alterations in the growth and chlorophyll content of Erythrina crista-galli plants in response to flooding and to evaluate possible photosystem II (PSII) damage through chlorophyll a fluorescence transient analysis using the JIP test. Plants cultured from seeds were grown in 0.5-L pots in a greenhouse and transferred to 5-L pots. Two treatments were employed: ...
... BACKGROUND: Fruit development is controlled by plant hormones, but the role of hormone interactions during fruit ripening is poorly understood. Interactions between ethylene and the auxin indole-3-acetic acid (IAA) are likely to be crucial during the ripening process, since both hormones have been shown to be implicated in the control of ripening in a range of different fruit species. RESULTS: Gra ...
Sara Cogliati; Christian Frezza; Maria Eugenia Soriano; Tatiana Varanita; Ruben Quintana-Cabrera; Mauro Corrado; Sara Cipolat; Veronica Costa; Alberto Casarin; Ligia C. Gomes; Ester Perales-Clemente; Leonardo Salviati; Patricio Fernandez-Silva; Jose A. Enriquez; Luca Scorrano
apoptosis; cell growth; electron transport chain; lung function; mitochondrial membrane; protein synthesis; ultrastructure
Abstract:
... Respiratory chain complexes assemble into functional quaternary structures called supercomplexes (RCS) within the folds of the inner mitochondrial membrane, or cristae. Here, we investigate the relationship between respiratory function and mitochondrial ultrastructure and provide evidence that cristae shape determines the assembly and stability of RCS and hence mitochondrial respiratory efficiency ...
... Multiple uses of native plants are appropriate for neighborhood squares, workers’ gardens, green roofs and degraded areas, whereas the use of plants from hydromorphic environments applies to landscaping in legally protected areas of Brazil (permanent preservation areas and riparian forests), like phytoremediation plants from contaminated areas, generating products for crafts and floriculture. In t ...
... Gyraulus crista is often a dominant component of lentic freshwater snail communities because it may occur in densities of hundreds of individuals/m2 across continents and in lentic water types. A study on the occurrence and conchological variability of the shell of G. crista was carried out on five different types of substrates in the anthropogenic woodland ponds at thirteen study sites. In order ...
Caesalpinia; anticarcinogenic activity; nuclear magnetic resonance spectroscopy
Abstract:
... 1α-acetoxy-5α, 7β-dihydroxycassa-11,13(15)-diene-16,12-lactone, a new cassane-type diterpene was isolated from Caesalpinia crista. The structure of this compound was elucidated by analysis of NMR spectra, and the relative configuration was established by NOE experiment. The new compound was evaluated for antitumour activity against T47D, DU145 and showed significant inhibitory activities. ...
... The isoflavonoids coumestrol, genistein and daidzein have been isolated and identified by bioassay-guided fractionation from the acetone extract of Erythrina crista galli young twigs infected with Phomopsis sp. These compounds showed antimicrobial activity against Bacillus brevis (MIC values 16.3, 64.8 and 137.8 μm, respectively). This is the first time that coumestrol, besides lutein and n-nonaco ...
Orthoptera; animal organs; cilia; dendritic cells; electron microscopy; hearing
Abstract:
... The number of scolopidia of the crista acustica of the Tettigonioidea is species-specific. First electron microscopic investigations of these scolopidia show that each of them is composed of 4 cells: A bipolar sense cell, an enveloping cell, a scolopale cell and a cap cell. The dendrite of the sense cell terminates in a cilium which is anchored within the dendrite with a root, which divides into n ...
... Phytochemical study on the methanolic extract of Caesalpinia crista afforded two novel compounds, 2-hydroxytrideca-3,6-dienyl-pentanoate and octacosa-12,15-diene along with known compounds 3- O -methylellagic acid 3′ O -α-rhamnopyranoside, β-sitosterol and sucrose. Compound 3- O -methylellagic acid 3′ O -α-rhamnopyranoside is reported for the first time from the plant. Molecular structures, of iso ...
Erythrina crista-galli, etc ; anthocyanins; biosynthesis; catalytic activity; cobalt; copper; corolla; cyanidin; enzyme substrates; flavonols; glycosyltransferases; high performance liquid chromatography; isoelectric point; molecular weight; nuclear magnetic resonance spectroscopy; pH; paper chromatography; zinc; Show all 18 Subjects
Abstract:
... The anthocyanins in the brilliant crimson petals from Erythrina crista-galli L. were extracted with 10% AcOH, and the extract was purified by Amberlite XAD-7 column chromatography, paper chromatography, and preparative HPLC. The structure of a major anthocyanin was confirmed as cyanidin 3-O-sophoroside by 1H and 13C NMR and FABMS. Then, the catalysis of UDP-glucose: flavonoid 3-O-glucoside glucosy ...
Erythrina crista-galli, etc ; Diaporthe; common names; drug therapy; endophytes; internal transcribed spacers; medicinal plants; metabolites; traditional medicine; trees; Show all 10 Subjects
Abstract:
... Erythrina crista-galli (Fabaceae) is used in Argentinean ethnopharmacology as anti-inflammatory medication, narcotic, desinfectant, and for the treatment of wounds. The common name of the tree is “ceibo“ or coral tree. The dominating endophytes in E. crista-galli all belong to the genus Phomopsis as identified by microscopic features and the analysis of their ITS sequences. To investigate a possib ...
Aditi Sood; Danny Vijey Jeyaraju; Julien Prudent; Alexandre Caron; Philippe Lemieux; Heidi May McBride; Mathieu Laplante; Katalin Tóth; Luca Pellegrini
... Hepatic metabolism requires mitochondria to adapt their bioenergetic and biosynthetic output to accompany the ever-changing anabolic/catabolic state of the liver cell, but the wiring of this process is still largely unknown. Using a postprandial mouse liver model and quantitative cryo-EM analysis, we show that when the hepatic mammalian target of rapamycin complex 1 (mTORC1) signaling pathway dise ...
... Mitochondria were isolated using sorbitol and high buffer concentration in the medium. X-ray diffraction patterns arising from the mitochondrial cristae-membrane were recorded in the fully dried state and in two different states in humidity. The Q-function evaluation of these X-ray diffraction diagrams resulted in electron density cross-section profiles, which consist of two main peaks of opposit ...
Crista Madison, et al. ; Doug J Hinchliffe; Anthony De Lucca; Brian Condon; Janet O’Regan; Julie Clemmons; Linghe Zeng; Richard K Byler; Michael Reynolds; Hiram Allen; Michael Santiago Cintron; Show all 11 Authors
... A total of seven source fiber types were selected for use in the manufacturing of nonwoven roll goods: polyester; polypropylene; rayon; greige cotton from two sources; mechanically cleaned greige cotton; and scoured and bleached cotton. The microbial burden of each source fiber was measured as a preliminary assessment of microbial contamination using heterotrophic spread plate counts. Greige cotto ...
... BACKGROUND: Cytokinins are known to play an important role in fruit set and early fruit growth, but their involvement in later stages of fruit development is less well understood. Recent reports of greatly increased cytokinin concentrations in the flesh of ripening kiwifruit (Actinidia deliciosa (A. Chev.) C.F. Liang & A.R. Ferguson) and grapes (Vitis vinifera L.) have suggested that these hormone ...
Crista D. Thomas, et al. ; Brad A. Haubrich; Emily K. Collins; Alicia L. Howard; Qian Wang; William J. Snell; Matthew B. Miller; Stephanie K. Pleasant; W. David Nes; Show all 9 Authors
... Sterol C24-methyltransferases (SMTs) constitute a group of sequence-related proteins that catalyze the pattern of sterol diversity across eukaryotic kingdoms. The only gene for sterol alkylation in green algae was identified and the corresponding catalyst from Chlamydomonas reinhardtii (Cr) was characterized kinetically and for product distributions. The properties of CrSMT were similar to those p ...
... Membrane architecture is crucially important for mitochondrial function and integrity. The MICOS complex is located at crista junctions and determines cristae membrane morphology and the formation of crista junctions. Here we provide data of the bona fide MICOS subunit MIC26 for determining cristae morphology. MiRNA-mediated downregulation of MIC26 results in higher protein levels of MIC27 and in ...
... 4- tert -Butyl-4′-methoxydibenzoylmethane (BMDM) is a widely used ultraviolet A filter. In this work, we have studied the effect of chlorine and dissolved organic matter (DOM) concentrations on the stability of UV filter (BMDM) present in two commercial sunscreen cream formulations in water. An experimental design was used to assess the effect of the two experimental factors on the degradation of ...