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1. Analysis of pesticides, veterinary drugs, and environmental contaminants in goat and lamb by the QuEChERSER mega-method

Author:
Alan R. Lightfield,   et al.   ; Ederina Ninga; Steven J. Lehotay; Yelena Sapozhnikova; Gary D. Strahan; Sergio H. Monteiro;     Show all 6 Authors
Source:
Analytical methods 2022 v.14 no.28 pp. 2761-2770
ISSN:
1759-9679
Subject:
automation; chemical species; data quality; gas chromatography; goats; labor; liquids; metabolites; muscles; pollution; polychlorinated biphenyls; sheep; tandem mass spectrometry; trade
Abstract:
... Analysis of chemical residues in foods is a big challenge for developing countries due to lack of financial and professional resources needed to meet international quality standards for trade. However, the implementation of simple multiclass, multi-residue methods in monitoring programs can provide significant benefits to save cost, time, and labor. The aim of this project was to investigate the “ ...
DOI:
10.1039/d2ay00713d

2. Validation of the QuEChERSER mega-method for the analysis of pesticides, veterinary drugs, and environmental contaminants in tilapia (Oreochromis Niloticus)

Author:
Alan R. Lightfield​,   et al.   ; Sergio H. Monteiro; Steven J. Lehotay; Yelena Sapozhnikova; Ederina Ninga; Graziela C.R. Moura Andrade;     Show all 6 Authors
Source:
Food additives & contaminants 2022 v.39 no.4 pp. 699-709
ISSN:
1944-0057
Subject:
Oreochromis niloticus; automation; chemical species; fish; food safety; freeze drying; gas chromatography; metabolites; muscles; pollution; polychlorinated biphenyls; robots; tandem mass spectrometry; ultra-performance liquid chromatography
Abstract:
... Diverse food safety programmes around the world are designed to help ensure production of safe food. To meet this need, the development and implementation of more efficient and effective analytical methods to monitor residues (pesticides and veterinary drugs) and contaminants in food is important. In this study, we report the validation results for a simple high-throughput mega-method for residual ...
Handle:
10113/7709667
DOI:
10.1080/19440049.2021.2020911

3. Validation of a high-throughput method for analysis of pesticide residues in hemp and hemp products

Author:
Alan R. Lightfield,   et al.   ; Nicolás Michlig; Steven J. Lehotay; Horacio Beldoménico; María Rosa Repetti;     Show all 5 Authors
Source:
Journal of chromatography 2021 v.1645 pp. 462097
ISSN:
0021-9673
Subject:
acetonitrile; automation; cannabinoids; centrifugation; chemical species; flowers; gas chromatography; hemp; metabolites; oils; pesticides; solvents; tandem mass spectrometry; ultra-performance liquid chromatography
Abstract:
... Hemp has been an agricultural commodity for millennia, and it has been undergoing a resurgence in interest and production due to its high content of cannabinoids, protein, fiber and other ingredients. For legal possession and use throughout the USA, hemp and hemp products must have delta-9-tetrahydrocannabinol (THC) concentration < 0.3%. As with most crops, pesticides may be applied when farming h ...
DOI:
10.1016/j.chroma.2021.462097

4. Comparison of four different multiclass, multiresidue sample preparation methods in the analysis of veterinary drugs in fish and other food matrices

Author:
Alan R. Lightfield,   et al.   ; Steven J. Lehotay;     Show all 2 Author
Source:
Analytical and bioanalytical chemistry 2021 v.413 no.12 pp. 3223-3241
ISSN:
1618-2642
Subject:
Food and Drug Administration; USDA; catfish; cattle; chemical species; food inspection; liquid chromatography; metabolites; muscles; reference standards; tandem mass spectrometry; veterinary drugs; China
Abstract:
... In 2018, AOAC International issued Standard Method Performance Requirements (SPMR) 2018.010 - Screening and Identification Method for Regulated Veterinary Drug Residues in Food. In response, we compared 4 different multiresidue methods of sample preparation using the same analytical method entailing ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). Tilapia was cho ...
DOI:
10.1007/s00216-021-03259-x

5. Assessment of Test Portion Sizes after Sample Comminution with Liquid Nitrogen in an Improved High-Throughput Method for Analysis of Pesticide Residues in Fruits and Vegetables

Author:
Alan R. Lightfield,   et al.   ; Steven J. Lehotay; Nicolás Michlig;     Show all 3 Authors
Source:
Journal of agricultural and food chemistry 2020 v.68 no.5 pp. 1468-1479
ISSN:
1520-5118
Subject:
apples; automation; broccoli; bulk commodities; cucumbers; data quality; freeze drying; fruits; gas chromatography; grapes; green beans; liquid chromatography; liquid nitrogen; magnesium sulfate; monitoring; peaches; pesticide residues; pesticides; portion size; quality control; reference standards; risk assessment; risk reduction; sodium chloride; squashes; standard deviation; tandem mass spectrometry; tomatoes; uncertainty
Abstract:
... In this study, sample processing of bulk commodities using an efficient one-step comminution procedure with liquid nitrogen (LN₂) was devised and assessed in the analysis of pesticide residues in fruits and vegetables. LN₂ was added to the fresh samples from a tank by opening a valve, and the standard food chopper was kept in a laboratory hood to reduce safety risks. Test portions of four replicat ...
DOI:
10.1021/acs.jafc.9b07685

6. High-Throughput Mega-Method for the Analysis of Pesticides, Veterinary Drugs, and Environmental Contaminants by Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry and Robotic Mini-Solid-Phase Extraction Cleanup + Low-Pressure Gas Chromatography–Tandem Mass Spectrometry, Part 2: Catfish

Author:
Alan R. Lightfield,   et al.   ; Ederina Ninga; Yelena Sapozhnikova; Steven J. Lehotay; Sergio H. Monteiro;     Show all 5 Authors
Source:
Journal of agricultural and food chemistry 2020 v.69 no.4 pp. 1169-1174
ISSN:
1520-5118
Subject:
European Union; automation; catfish; chemical species; food chemistry; gas chromatography; liquid chromatography; metabolites; muscles; pollution; polychlorinated biphenyls; robots; tandem mass spectrometry
Abstract:
... The goal of this study was to develop and validate a new method for simultaneous determination of 106 veterinary drugs and 227 pesticides and their metabolites plus 16 polychlorinated biphenyls (PCBs) at and below their regulatory levels established for catfish muscle in the European Union and U.S.A. To do this, two different QuEChERS-based methods for veterinary drugs and pesticides and PCBs were ...
DOI:
10.1021/acs.jafc.0c00995

7. Simultaneous analysis of aminoglycosides with many other classes of drug residues in bovine tissues by ultrahigh-performance liquid chromatography–tandem mass spectrometry using an ion-pairing reagent added to final extracts

Author:
Alan R. Lightfield,   et al.   ; Steven J. Lehotay;     Show all 2 Author
Source:
Analytical and bioanalytical chemistry 2018 v.410 no.3 pp. 1095-1109
ISSN:
1618-2642
Subject:
aminoglycoside antibiotics; aminoglycosides; capital; cattle; drug residues; food animals; kidneys; liquid chromatography; liver; monitoring; muscle tissues; tandem mass spectrometry; veterinary drugs
Abstract:
... The way to maximize scope of analysis, sample throughput, and laboratory efficiency in the monitoring of veterinary drug residues in food animals is to determine as many analytes as possible as fast as possible in as few methods as possible. Capital and overhead expenses are also reduced by using fewer instruments in the overall monitoring scheme. Traditionally, the highly polar aminoglycoside ant ...
DOI:
10.1007/s00216-017-0688-9

8. High-Throughput Mega-Method for the Analysis of Pesticides, Veterinary Drugs, and Environmental Contaminants by Ultra-High-Performance Liquid Chromatography−Tandem Mass Spectrometry and Robotic Mini-Solid-Phase Extraction Cleanup + Low-Pressure Gas Chromatography−Tandem Mass Spectrometry, Part 1: Beef

Author:
Alan R. Lightfield,   et al.   ; Sergio H. Monteiro; Steven J. Lehotay; Yelena Sapozhnikova; Ederina Ninga;     Show all 5 Authors
Source:
Journal of agricultural and food chemistry 2020 v.69 no.4 pp. 1159-1168
ISSN:
1520-5118
Subject:
automation; beef; cattle; chemical pollutants; chemical species; cost effectiveness; food chemistry; freeze drying; gas chromatography; international trade; labor; liquid chromatography; metabolites; muscles; pollution; polychlorinated biphenyls; reference standards; risk assessment; robots; solid phase extraction; standard deviation; tandem mass spectrometry
Abstract:
... In this work, a new mega-method of sample preparation called “QuEChERSER” (more than QuEChERS) is being presented for the first time. Fast, efficient, and cost-effective analysis of chemical contaminants in meat is useful for international trade, domestic monitoring, risk assessment, and other purposes. The goal of this study was to develop and validate a simple high-throughput mega-method for res ...
DOI:
10.1021/acs.jafc.0c00710

9. Validation of a streamlined multiclass, multiresidue method for determination of veterinary drug residues in bovine muscle by liquid chromatography–tandem mass spectrometry

Author:
Alan R. Lightfield,   et al.   ; Marilyn J. Schneider; Steven J. Lehotay;     Show all 3 Authors
Source:
Analytical and bioanalytical chemistry 2015 v.407 no.15 pp. 4423-4435
ISSN:
1618-2642
Subject:
cattle; drug residues; filtration; ionization; liquid chromatography; monitoring; multiresidue analysis; muscles; solid phase extraction; spectrometers; tandem mass spectrometry; veterinary drugs
Abstract:
... Multiclass, multiresidue methods are becoming increasingly popular in regulatory monitoring programs due to their increased analytical scope and laboratory efficiency. In this work, we report the development and validation of a new high-throughput analytical method to monitor up to 131 veterinary drug residues, representing at least 13 different classes, in bovine muscle. This novel method streaml ...
Handle:
10113/61044
DOI:
10.1007/s00216-014-8386-3

10. Structural characterization of product ions by electrospray ionization and quadrupole time-of-flight mass spectrometry to support regulatory analysis of veterinary drug residues in foods

Author:
Alan R. Lightfield,   et al.   ; Lucia Geis-Asteggiante; Alberto Nuñez; Steven J. Lehotay;     Show all 4 Authors
Source:
Rapid Communications in Mass Spectrometry 2014 v.28 no.10 pp. 1061-1081
Subject:
adrenal cortex hormones; beta-lactams; drug residues; fluoroquinolones; foods; ionization; ions; liquid chromatography; macrolides; mass spectrometry; monitoring; nonsteroidal anti-inflammatory agents; sulfonamides; tetracyclines; veterinary drugs
Abstract:
... RATIONALE: Monitoring of veterinary drug residues in foods is often conducted using liquid chromatography – tandem mass spectrometry (LC-MS/MS). Results have high economic stakes for producers, but the ions monitored are usually selected due to signal intensities without structural interpretation. In this study, the ion transitions were characterized by high-resolution mass spectrometry METHOD: Th ...
Handle:
10113/58696
DOI:
10.1002/rcm.6871
PubMed:
24711270

11. Ruggedness testing and validation of a practical analytical method for >100 veterinary drug residues in bovine muscle by ultrahigh performance liquid chromatography–tandem mass spectrometry.

Author:
Alan R. Lightfield,   et al.   ; Lucía Geis-Asteggiante; Steven J. Lehotay;     Show all 3 Authors
Source:
Journal of Chromatography A 2012 v.1258 pp. 43-54
ISSN:
0021-9673
Subject:
anthelmintics; cattle; drug residues; ionization; mass spectrometry; meat; monitoring; muscles; physicochemical properties; screening; solid phase extraction; system optimization; ultra-performance liquid chromatography; veterinary drugs; United States
Abstract:
... In this study, optimization, extension, and validation of a streamlined, qualitative and quantitative multiclass, multiresidue method was conducted to monitor >100 veterinary drug residues in meat using ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS). Optimization centered on extensive ruggedness evaluation of the method. Various clean-up sorbents were tested and ...
Handle:
10113/57430
DOI:
10.1016/j.chroma.2012.08.020

12. Rapid analysis of aminoglycoside antibiotics in bovine tissues using disposable pipette extraction and ultrahigh performance liquid chromatography - tandem mass spectrometry

Author:
Alan R. Lightfield,   et al.   ; Steven J. Lehotay; Katerina Mastovska; Alberto Nuñez; Terry Dutko; Chilton Ng; Louis Bluhm;     Show all 7 Authors
Source:
Journal of chromatography 2013 v.1313 pp. 103-112
ISSN:
0021-9673
Subject:
amikacin; apramycin; cattle; detection limit; gentamicin; kidneys; liver; mass spectrometry; muscle tissues; neomycin; screening; spectinomycin; streptomycin; tobramycin; ultra-performance liquid chromatography; United States
Abstract:
... A high-throughput qualitative screening and identification method for 9 aminoglycosides of regulatory interest has been developed, validated, and implemented for bovine kidney, liver, and muscle tissues. The method involves extraction at previously validated conditions, cleanup using disposable pipette extraction, and analysis by a 3 min ultrahigh-performance liquid chromatography – tandem mass sp ...
Handle:
10113/57987
DOI:
10.1016/j.chroma.2013.08.103