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- Aldema-Ramos, Mila L., et al. Show all 4 Authors
- Journal of the American Leather Chemists Association 2008 v.103 no.10 pp. 324-329
- animal proteins; polyacrylamide gel electrophoresis; cattle; antibodies; Western blotting; enzyme-linked immunosorbent assay; chondroitin sulfate; leather; collagenase; mass spectrometry; proteoglycans
- ... The present work attempts to develop a more accurate and reliable assay for decorin concentration in hides as they are processed to leather. The ELISA technique previously developed for decorin analysis is further improved and optimized by treating the samples with chondroitinase-ABC after dialyzing them in the presence of collagenase. Some newer techniques developed and adapted include SDS-PAGE a ...