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- Alvarez-Díaz, Juan C., et al. Show all 3 Authors
- Journal of virological methods 2019 v.266 pp. 41-48
- Chrysanthemum; RNA; complementary DNA; diagnostic sensitivity; diagnostic specificity; diagnostic techniques; farms; pathogens; protocols; reverse transcriptase polymerase chain reaction; viruses
- ... A novel duplex RT-PCR assay for simultaneous detection of TSWV and CSVd in chrysanthemums was developed. Previous reported primers for amplification of TSWV and CSVd were used and a novel pair of primers for CSVd was designed to improve duplex amplification compatibility. Sensitivity and efficiency of the previous reported and novel primers for CSVd were assessed. Then, the sensitivity of the comb ...