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- Anji, Antje, et al. Show all 2 Author
- Analytical biochemistry 2019 v.566 pp. 20-22
- alkylation; alpha-glucosidase; antibodies; brain; mass spectrometry; mice; molecular weight; proteins; two-dimensional gel electrophoresis; urea
- ... Protein reduction and alkylation is routinely used for analysis of protein dimers and protein complexes in cell fractions using two dimensional gel electrophoresis and mass spectrometry. To resolve the heterogeneity of a high molecular weight protein band that is highlighted by an antibody to the beta subunit of alpha glucosidase II (GIIβ), we performed reduction and alkylation of cytosolic protei ...