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- Author:
- Ario de Marco, et al. ; Sandra Oloketuyi; Giulia Annovi; Show all 3 Authors
- Source:
- International journal of biological macromolecules 2020 v.156 pp. 869-873
- ISSN:
- 0141-8130
- Subject:
- acrylamides; agarose; biomarkers; gel electrophoresis; gels; horseradish; peroxidase; protein isoforms; staining
- Abstract:
- ... A recently published method to separate protein isoforms by means of a flat-bed agarose native gel was adapted for identifying simultaneously both acid and basic isoforms of plant peroxidases. These were evidenced by in situ activity staining using alternative substrates for which the isoforms showed specific preference. Such approach allowed the detection of a significantly higher number of horse ...
- DOI:
- 10.1016/j.ijbiomac.2020.04.058
- https://doi.org/10.1016/j.ijbiomac.2020.04.058
- Author:
- Ario de Marco, et al. ; Lidija Filipović; Milica Spasojević; Radivoje Prodanović; Aleksandra Korać; Suzana Matijaševic; Goran Brajušković; Milica Popović; Show all 8 Authors
- Source:
- New biotechnology 2022 v.69 pp. 36-48
- ISSN:
- 1871-6784
- Subject:
- biomarkers; biotechnology; cell culture; chromatography; composite polymers; culture media; exosomes; fractionation; humans; lipoproteins; porous media
- Abstract:
- ... Correct elucidation of physiological and pathological processes mediated by extracellular vesicles (EV) is highly dependent on the reliability of the method used for their purification. Currently available chemical/physical protocols for sample fractionation are time-consuming, often scarcely reproducible and their yields are low. Immuno-capture based approaches could represent an effective purifi ...
- DOI:
- 10.1016/j.nbt.2022.03.001
- https://doi.org/10.1016/j.nbt.2022.03.001
- Author:
- Ario de Marco, et al. ; Serena D'Agostino; Elisa Mazzega; Katja Praček; Sara Piccinin; Flavia Pivetta; Michela Armellin; Sara Fortuna; Roberta Maestro; Show all 9 Authors
- Source:
- International journal of biological macromolecules 2022 v.194 pp. 24-31
- ISSN:
- 0141-8130
- Subject:
- cell viability; humans; llamas; phosphorylation; serine; therapeutics
- Abstract:
- ... Twist1 promote the bypass of p53 response by interacting with p53 and facilitating its MDM2-mediated degradation. We reasoned that reagents able to interfere with the p53:Twist1 complex might alleviate Twist1 inhibitory effect over p53, thus representing potential therapeutic tools in p53 wild type tumors. From a pre-immune library of llama nanobodies (VHH), we isolated binders targeting the p53 C ...
- DOI:
- 10.1016/j.ijbiomac.2021.11.160
- https://doi.org/10.1016/j.ijbiomac.2021.11.160
- Author:
- Ario de Marco, et al. ; Oginni Gbenga Folorunsho; Sandra Folarin Oloketuyi; Elisa Mazzega; Hanna Budasheva; Alfred Beran; Marina Cabrini; Dorota Korte; Mladen Franko; Show all 9 Authors
- Source:
- Applied biochemistry and biotechnology 2021 v.193 no.9 pp. 2729-2741
- ISSN:
- 0273-2289
- Subject:
- Microcystis aeruginosa; bacteriophages; biotechnology; colorimetry; cross reaction; detection limit; diagnostic techniques; enzyme-linked immunosorbent assay; fluorescence; fluorescent antibody technique; green fluorescent protein; microalgae; peroxidase; spectroscopy; toxicity
- Abstract:
- ... Nanobodies against cell surface antigens of toxic cyanobacteria Microcystis aeruginosa were recovered by whole-cell biopanning of a naïve phage display library of nanobodies. Six unique sequences were identified and three sub-cloned and purified as fusion immunoreagents together with either green fluorescent protein or AviTag to be used for diagnostics. The yields of nanobody constructs were in th ...
- DOI:
- 10.1007/s12010-021-03552-6
- https://doi.org/10.1007/s12010-021-03552-6
- Author:
- Ario de Marco, et al. ; Sandra Oloketuyi; Elisa Mazzega; Janez Zavašnik; Kingkan Pungjunun; Kurt Kalcher; Eda Mehmeti; Show all 7 Authors
- Source:
- Biosensors & bioelectronics 2020 v.154 pp. 112052
- ISSN:
- 0956-5663
- Subject:
- Alexandrium minutum; brackish water; chemical bonding; cysteine; detection limit; diagnostic techniques; dialysis; dielectric spectroscopy; electrochemistry; glassy carbon electrode; immunosensors; microalgae; nanogold; scanning electron microscopy; seawater; toxicity; voltammetry
- Abstract:
- ... In this work an electrochemical immunosensor for the toxic microalgae Alexandrium minutum (A. minutum AL9T) detection is described. A glassy carbon electrode (GCE) was modified by depositing gold nanoparticles followed by L-cysteine for obtaining a self-assembled monolayer. The SpyTagged nanobody C1, specific for the A. minutum toxic strain AL9T, was then covalently immobilized via SpyCatcher on t ...
- DOI:
- 10.1016/j.bios.2020.112052
- https://doi.org/10.1016/j.bios.2020.112052
- Author:
- Ario de Marco, et al. ; Elisa Mazzega; Alfred Beran; Marina Cabrini; Show all 4 Authors
- Source:
- Harmful algae 2019 v.82 pp. 44-51
- ISSN:
- 1568-9883
- Subject:
- Alexandrium minutum; algal blooms; antibodies; bacteria; ballast water; coastal water; cross reaction; diagnostic techniques; epitopes; flow cytometry; fluorescence microscopy; fluorescent dyes; human resources; indigenous species; laws and regulations; light microscopy; microalgae; models; phytoplankton; poisonous algae; quantitative polymerase chain reaction; toxicity; water management
- Abstract:
- ... At the present, the identification of planktonic species in coastal water is still a time intensive process performed by highly trained personnel that relies either on qPCR or on light microscopy observation and in vitro culturing. Furthermore, the increasing danger represented by Harmful Algal Blooms (HABs) inside phytoplankton community and the recent implementation of the legislation on ballast ...
- DOI:
- 10.1016/j.hal.2019.01.002
- https://doi.org/10.1016/j.hal.2019.01.002
- Author:
- Ario de Marco, et al. ; Giulio Bernardinelli; Sandra Oloketuyi; Salesia Franziska Werner; Elisa Mazzega; Björn Högberg; Show all 6 Authors
- Source:
- New biotechnology 2020 v.56 pp. 1-8
- ISSN:
- 1871-6784
- Subject:
- Alexandrium minutum; DNA; amino acids; antibodies; antigen detection; antigens; binding capacity; catalytic activity; chemical bonding; colorimetry; cost effectiveness; electrochemistry; enzyme substrates; enzyme-linked immunosorbent assay; nanoparticles; peroxidase; poisonous algae; stoichiometry; streptavidin
- Abstract:
- ... Hemin-utilizing G-quadruplex DNAzymes with peroxidase-like (POX) activity are widely used as signal reporters in biosensing technology. However, their application to protein detection has been mostly limited to sandwich-type assays involving streptavidin or nanoparticles as indirect bridging platforms between DNAzymes and antibodies. Herein, we describe the generation of a compact, covalently DNAz ...
- DOI:
- 10.1016/j.nbt.2019.10.009
- https://doi.org/10.1016/j.nbt.2019.10.009
- Author:
- Ario de Marco, et al. ; Sandra Oloketuyi; Carina Dilkaute; Elisa Mazzega; Joachim Jose; Show all 5 Authors
- Source:
- Applied microbiology and biotechnology 2019 v.103 no.11 pp. 4443-4453
- ISSN:
- 0175-7598
- Subject:
- Escherichia coli; antibodies; bacteria; cytoplasm; diagnostic techniques; eukaryotic cells; flow cytometry; fluorescence; microalgae; neoplasm cells; proteins; toxicity
- Abstract:
- ... The availability of preimmune libraries of antibody fragments allows for the fast generation of binders which can be expressed in both eukaryotic and prokaryotic systems. We exploited the recombinant nature of antibody fragments to demonstrate the possibility of expressing them as functional proteins displayed on the surface of Escherichia coli and by such a way to generate living reagents ready-t ...
- DOI:
- 10.1007/s00253-019-09823-x
- https://doi.org/10.1007/s00253-019-09823-x
- Author:
- Ario de Marco, et al. ; Miguel A. Soler; Barbara Medagli; Marta S. Semrau; Paola Storici; Gregor Bajc; Alessandro Laio; Sara Fortuna; Show all 8 Authors
- Source:
- Chemical communications 2019 v.55 no.93 pp. 14043-14046
- ISSN:
- 1364-548X
- Subject:
- algorithms; antibodies; bacteriophages; binding capacity; chemical compounds; chemical reactions; computer simulation; molecular dynamics; mutants; mutation; peptides; solvents
- Abstract:
- ... We present an in silico mutagenetic protocol for improving the binding affinity of single domain antibodies (or nanobodies, VHHs). The method iteratively attempts random mutations in the interacting region of the protein and evaluates the resulting binding affinity towards the target by scoring, with a collection of scoring functions, short explicit solvent molecular dynamics trajectories of the b ...
- DOI:
- 10.1039/c9cc06182g
- https://doi.org/10.1039/c9cc06182g
- Author:
- Ario de Marco, et al. ; Laura Sánchez-García; Eric Voltà-Durán; Eloi Parladé; Elisa Mazzega; Alejandro Sánchez-Chardi; Naroa Serna; Hèctor López-Laguna; Mara Mitstorfer; Ugutz Unzueta; Esther Vázquez; Antonio Villaverde; Show all 12 Authors
- Source:
- ACS applied materials & interfaces 2021 v.13 no.25 pp. 29406-29415
- ISSN:
- 1944-8252
- Subject:
- oligomerization; ricin; therapeutics; toxins
- Abstract:
- ... Nanobodies represent valuable tools in advanced therapeutic strategies but their small size (∼2.5 × ∼ 4 nm) and limited valence for interactions might pose restrictions for in vivo applications, especially regarding their modest capacity for multivalent and cooperative interaction. In this work, modular protein constructs have been designed, in which nanobodies are fused to protein domains to prov ...
- DOI:
- 10.1021/acsami.1c08092
- https://doi.org/10.1021/acsami.1c08092
- Author:
- Ario de Marco, et al. ; Daniele Ubbiali; Marco Orlando; Matic Kovačič; Claudio Iacobucci; Marta S. Semrau; Gregor Bajc; Sara Fortuna; Gregor Ilc; Barbara Medagli; Sandra Oloketuyi; Paola Storici; Andrea Sinz; Rita Grandori; Show all 14 Authors
- Source:
- International journal of biological macromolecules 2021 v.182 pp. 502-511
- ISSN:
- 0141-8130
- Subject:
- antibody binding sites; crosslinking; data collection; epitopes; erbB-2 receptor; mass spectrometry; molecular dynamics; peptides
- Abstract:
- ... High-resolution structural data of complexes between antibodies and membrane receptors still represent a demanding task. In this study, we used complementary sets of experimental data to obtain a structural model of the complex formed by the human epidermal growth factor receptor 2 (HER2) and its specific nanobody A10. First we identified by NMR the residues that bind or rearrange as a consequence ...
- DOI:
- 10.1016/j.ijbiomac.2021.04.032
- https://doi.org/10.1016/j.ijbiomac.2021.04.032
- Author:
- Ario de Marco
- Source:
- Critical reviews in biotechnology 2013 v.33 no.1 pp. 40-48
- ISSN:
- 1549-7801
- Subject:
- animals; cytotoxicity; dimerization; epitopes; immunization; monoclonal antibodies; peptides; phages; proteins; recombinant antibodies; signal transduction; synergism
- Abstract:
- ... The availability of binders to different functional domains of the same protein or to physiologically co-operating proteins allows for the simultaneous inhibition of independent downstream signaling pathways. This multi-target approach represents a promising therapeutic strategy, as demonstrated in the case of the synergistic effect of anti-Her2 treatment based on the combined use of the trastuzum ...
- DOI:
- 10.3109/07388551.2012.665353
- https://doi.org/10.3109/07388551.2012.665353
- Author:
- Ario de Marco, et al. ; Bert Dhondt; Jan Van Deun; Silke Vermaerke; Nicolaas Lumen; Olivier De Wever; An Hendrix; Show all 7 Authors
- Source:
- international journal of biochemistry & cell biology 2018 v.99 pp. 236-256
- ISSN:
- 1357-2725
- Subject:
- biomarkers; cell communication; cost effectiveness; early diagnosis; excretion; glomerular filtration rate; neoplasms; pathophysiology; prediction; prognosis; proteins; renal tubules; secretion; therapeutics; uncertainty; urine
- Abstract:
- ... Urine contains cellular elements, biochemicals, and proteins derived from glomerular filtration of plasma, renal tubule excretion, and urogenital tract secretions that reflect an individual's metabolic and pathophysiologic state. Despite intensive research into the discovery of urinary biomarkers to facilitate early diagnosis, accurate prognosis and prediction of therapy response in urological can ...
- DOI:
- 10.1016/j.biocel.2018.04.009
- https://doi.org/10.1016/j.biocel.2018.04.009
- Author:
- Ario de Marco, et al. ; Milica Popović; Marco Gregori; Dominik Vodnik; Mitja Ferlan; Tanja Mrak; Ines Štraus; Nathan G. McDowell; Hojka Kraigher; Show all 9 Authors
- Source:
- Canadian journal of forest research = 2017 v.47 no.11 pp. 1517-1526
- ISSN:
- 1208-6037
- Subject:
- Fagus sylvatica subsp. sylvatica; biomarkers; breeding; cell walls; drought; forest management; forest trees; free radical scavengers; glutathione peroxidase; metabolites; monitoring; phenolic compounds; photosynthesis; rain; risk; seedlings; soil temperature; superoxide dismutase; water deprivation
- Abstract:
- ... Drought is an environmental stress that impacts plant productivity. Projections show both an increase in intense rain events and a reduction in the number of rain days, conditions that leads to increased risk of drought. Consequently, the identification of molecular biomarkers suitable for evaluating the impact of water deprivation conditions on forest plant seedlings is of significant value for m ...
- DOI:
- 10.1139/cjfr-2016-0530
- https://doi.org/10.1139/cjfr-2016-0530
- Author:
- Ario de Marco, et al. ; Hèctor López-Laguna; Julieta M. Sánchez; José Vicente Carratalá; Mauricio Rojas-Peña; Laura Sánchez-García; Eloi Parladé; Alejandro Sánchez-Chardi; Eric Voltà-Durán; Naroa Serna; Olivia Cano-Garrido; Sandra Flores; Neus Ferrer-Miralles; Verónica Nolan; Nerea Roher; Ugutz Unzueta; Esther Vazquez; Antonio Villaverde; Show all 18 Authors
- Source:
- ACS sustainable chemistry & engineering 2021 v.9 no.36 pp. 12341-12354
- ISSN:
- 2168-0485
- Subject:
- biofabrication; biomedical research; green chemistry; polypeptides
- Abstract:
- ... We have developed a simple, robust, and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn²⁺ ions and His residues in His-tagged proteins. This approach results in a spontaneous a ...
- DOI:
- 10.1021/acssuschemeng.1c04256
- https://doi.org/10.1021/acssuschemeng.1c04256
- Author:
- Ario de Marco, et al. ; Milica Popović; Vid Šuštar; Jozica Gričar; Ines Štraus; Gregor Torkar; Hojka Kraigher; Show all 7 Authors
- Source:
- Canadian journal of forest research = 2015 v.46 no.1 pp. 58-66
- ISSN:
- 1208-6037
- Subject:
- Fagus sylvatica subsp. sylvatica; Pinus sylvestris; abiotic stress; biomarkers; breeding; cell walls; climate; crops; forest management; forest trees; forests; free radical scavengers; glutathione; metabolism; metabolites; models; monitoring; peroxidases; phenolic compounds; seedlings; superoxide dismutase; temperature; Central European region
- Abstract:
- ... Climate development models predict alterations that will critically influence plant metabolism in southern and central Europe. Although the molecular players involved in the response to climatic stress factors have been well described in crops, little information is available for forest tree species. Consequently, the identification of molecular biomarkers suitable for evaluating the actual impact ...
- DOI:
- 10.1139/cjfr-2015-0274
- https://doi.org/10.1139/cjfr-2015-0274
- Author:
- Ario de Marco, et al. ; Yoav Peleg; Renaud Vincentelli; Brett M. Collins; Kai-En Chen; Emma K. Livingstone; Saroja Weeratunga; Natalya Leneva; Qian Guo; Kim Remans; Kathryn Perez; Gro E.K Bjerga; Øivind Larsen; Ondřej Vaněk; Ondřej Skořepa; Sophie Jacquemin; Arnaud Poterszman; Svend Kjær; Evangelos Christodoulou; Shira Albeck; Orly Dym; Elena Ainbinder; Tamar Unger; Anja Schuetz; Susann Matthes; Michael Bader; Paola Storici; Marta S. Semrau; Peggy Stolt-Bergner; Christian Aigner; Sabine Suppmann; Adi Goldenzweig; Sarel J Fleishman; Show all 33 Authors
- Source:
- Journal of molecular biology 2021 v.433 no.13 pp. 166964
- ISSN:
- 0022-2836
- Subject:
- Escherichia coli; algorithms; heterologous gene expression; humans; molecular biology; protein synthesis; stem cell factor; thermal stability; Europe
- Abstract:
- ... Recent years have seen a dramatic improvement in protein-design methodology. Nevertheless, most methods demand expert intervention, limiting their widespread adoption. By contrast, the PROSS algorithm for improving protein stability and heterologous expression levels has been successfully applied to a range of challenging enzymes and binding proteins. Here, we benchmark the application of PROSS as ...
- DOI:
- 10.1016/j.jmb.2021.166964
- https://doi.org/10.1016/j.jmb.2021.166964
- Author:
- Ario de Marco, et al. ; Ronan Crepin; Gianluca Veggiani; Selma Djender; Anne Beugnet; François Planeix; Christophe Pichon; Sandrine Moutel; Sebastian Amigorena; Franck Perez; Nicolae Ghinea; Show all 11 Authors
- Source:
- Biochemical and biophysical research communications 2017 pp. -
- ISSN:
- 0006-291X
- Subject:
- G-protein coupled receptors; biomarkers; cyclic AMP; cytoplasm; diagnostic techniques; epitopes; follicle-stimulating hormone; follicle-stimulating hormone receptors; neoplasms; recombinant antibodies; spermatogenesis; therapeutics
- Abstract:
- ... Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrat ...
- DOI:
- 10.1016/j.bbrc.2017.10.036
- https://doi.org/10.1016/j.bbrc.2017.10.036
- Author:
- Ario de Marco, et al. ; Chiara Martinelli; Emanuela Colombo; Daniele Piccini; Cristina Sironi; Pier Giuseppe Pelicci; Show all 6 Authors
- Source:
- Biotechnology reports 2014 v.3 pp. 27-33
- ISSN:
- 2215-017X
- Subject:
- antigens; biotechnology; cytoplasm; mutants; mutation; myeloid leukemia; nuclear localization signals; protein synthesis; protein transport; proteins
- Abstract:
- ... The cytoplasmic accumulation of NPM1 (NPMc+) is found in acute myeloid leukemia (AML) with NPM1 mutation. NPM1 must shuttle between nucleus and cytoplasm to assure physiological protein synthesis and, therefore, the elimination of NPMc+ is not a suitable therapeutic option. We isolated, characterized, and produced a functional scFv intrabody fused to nuclear localization signal(s) (NLS) that does ...
- DOI:
- 10.1016/j.btre.2014.05.008
- PubMed:
- 28626645
- PubMed Central:
- PMC5466097
- https://doi.org/10.1016/j.btre.2014.05.008
- Author:
- Ario de Marco, et al. ; Tao Zou; Fatimata Dembele; Anne Beugnet; Lucie Sengmanivong; Sylvain Trepout; Sergio Marco; Min-Hui Li; Show all 8 Authors
- Source:
- Journal of biotechnology 2015 v.214 pp. 147-155
- ISSN:
- 0168-1656
- Subject:
- biodegradability; breast neoplasms; composite polymers; confocal microscopy; drugs; flow cytometry; fluorescein; in vivo studies; light scattering; nanoparticles; neoplasm cells; pH; particle size; polyethylene glycol; receptors; transmission electron microscopy; zeta potential
- Abstract:
- ... We prepared and characterized polymersomes functionalized with nanobodies (VHHs) on the basis of biocompatible, biodegradable and FDA-approved poly(ethylene glycol)-block-poly(ϵ-caprolactone) (PEG-b-PCL). Fluorescein isothiocyanate (FITC) and N-beta-maleimidopropyl-oxysuccinimide ester were allowed reacting with H2N-PEG-b-PCL to produce FITC and maleimide (Mal) functionalized copolymers, Mal-PEG-b ...
- DOI:
- 10.1016/j.jbiotec.2015.09.034
- https://doi.org/10.1016/j.jbiotec.2015.09.034