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1. Structure, function and regulation of the thermostable direct hemolysin (TDH) in pandemic Vibrio parahaemolyticus

Author:
Cai, Qin,   et al.   ; Zhang, Yiquan;     Show all 2 Author
Source:
Microbial pathogenesis 2018 v.123 pp. 242-245
ISSN:
0882-4010
Subject:
Vibrio parahaemolyticus; agar; cytotoxicity; gastroenteritis; genes; hemolysins; hemolysis; pandemic; pathogenesis; pathogens; thermal stability; transcription (genetics)
Abstract:
... Vibrio parahaemolyticus is a leading cause of seafood-associated bacterial gastroenteritis. The pathogen produces the thermostable direct hemolysin (TDH), which is the sole cause of the Kanagawa phenomenon (KP), a special β-type haemolysis in the Wagatsuma agar. TDH also exerts several other biological activities, the major includes lethal toxicity, cytotoxicity, and enterotoxicity. The structure ...
DOI:
10.1016/j.micpath.2018.07.021

2. Recyclable three-dimensional Ag nanorod arrays decorated with O-g-C3N4 for highly sensitive SERS sensing of organic pollutants

Author:
Han, Cai-Qin,   et al.   ; Qu, Lu-Lu; Geng, Zhi-Qin; Wang, Wen; Yang, Kai-Chun; Wang, Wei-Peng; Yang, Guo-Hai; Vajtai, Robert; Li, Da-Wei; Ajayan, Pulickel M.;     Show all 10 Authors
Source:
Journal of hazardous materials 2019 v.379 pp. 120823
ISSN:
0304-3894
Subject:
Raman spectroscopy; antibiotics; carbon nitride; catalytic activity; chemical species; graphene; irradiation; light; nanorods; nanosilver; pollutants; silver
Abstract:
... A three-dimensional (3D) substrate was developed by assembling a monolayer of graphitic carbon nitride (O-g-C3N4) on Ag nanorod arrays (Ag NRs) for sensitive and recyclable surface enhanced Raman scattering (SERS) detection. The prepared Ag NRs/O-g-C3N4 substrate not only generated a significant Raman enhancement effect as a result of the strong π-π stacking interaction between O-g-C3N4 and the an ...
DOI:
10.1016/j.jhazmat.2019.120823

3. Thin layer chromatography combined with surface-enhanced raman spectroscopy for rapid sensing aflatoxins

Author:
Han, Cai-Qin,   et al.   ; Qu, Lu-Lu; Jia, Qin; Liu, Chunyuan; Wang, Wen; Duan, Lingfeng; Yang, Guohai; Li, Haitao;     Show all 8 Authors
Source:
Journal of chromatography 2018 v.1579 pp. 115-120
ISSN:
0021-9673
Subject:
Raman spectroscopy; aflatoxin B1; aflatoxin B2; aflatoxin G1; aflatoxin G2; colloids; detection limit; gold; molds (fungi); peanuts; screening; spectrometers; thin layer chromatography
Abstract:
... A fast and facile method was developed for on-site detection of aflatoxins (AFs) in moldy agricultural products using thin layer chromatography combined with surface-enhanced Raman spectroscopy (TLC-SERS). Four different AFs were successfully separated by TLC and then a small portable Raman spectrometer, with gold colloids as the SERS-active substrate, was applied to identify the separated spots. ...
DOI:
10.1016/j.chroma.2018.10.024

4. Highly reproducible solid-phase extraction membrane for removal and surface-enhanced Raman scattering detection of antibiotics

Author:
Han, Cai-Qin,   et al.   ; Jia, Qin; Geng, Zhi-Qin; Liu, Yang; Wang, Wen; Yang, Guo-Hai; Li, Haitao; Qu, Lu-Lu;     Show all 8 Authors
Source:
Journal of materials science 2018 v.53 no.21 pp. 14989-14997
ISSN:
0022-2461
Subject:
Raman spectroscopy; activated carbon; adsorption; antibiotics; aqueous solutions; detection limit; filtration; nanosilver; quantitative analysis; rhodamines; solid phase extraction
Abstract:
... A disposable solid-phase extraction (SPE) membrane was developed for rapid removal and sensitive surface-enhanced Raman scattering (SERS) detection of antibiotics in water samples. The membrane was fabricated on commercially available SPE column by filtration of the activated carbon modified with silver nanoparticles (Ag NPs/AC). The prepared SPE membrane exhibited outstanding preconcentration abi ...
DOI:
10.1007/s10853-018-2745-y

5. Alantolactone, a sesquiterpene lactone, inhibits breast cancer growth by antiangiogenic activity via blocking VEGFR2 signaling

Author:
Cai, Qin‐Yi,   et al.   ; Liu, Ya‐Rong; Gao, Yun‐Ge; Luan, Xin; Guan, Ying‐Yun; Lu, Qin; Sun, Peng; Zhao, Mei; Fang, Chao;     Show all 9 Authors
Source:
Phytotherapy research 2018 v.32 no.4 pp. 643-650
ISSN:
0951-418X
Subject:
Inula helenium; angiogenesis; antineoplastic activity; breast neoplasms; chickens; chicks; chorioallantoic membrane; drugs; human umbilical vein endothelial cells; humans; medicinal plants; mice; phosphorylation; sesquiterpenoids; therapeutics; vascular endothelial growth factor receptor-2
Abstract:
... Alantolactone (ALA), a sesquiterpene lactone isolated from several medicinal plants such as Inula helenium, has been identified to have attractive anticancer activity. However, its role in the inhibition of angiogenesis during tumor development remains unclear. In this study, we found ALA can inhibit the proliferation, motility, migration, and tube formation of human umbilical vein endothelial cel ...
DOI:
10.1002/ptr.6004

6. One-Step Synthesis of Rox-DNA Functionalized CdZnTeS Quantum Dots for the Visual Detection of Hydrogen Peroxide and Blood Glucose

Author:
Cai, Qin,   et al.   ; Mao, Guobin; Wang, Fubing; Luo, Changliang; Ji, Xinghu; He, Zhike;     Show all 6 Authors
Source:
Analytical chemistry 2017 v.89 no.21 pp. 11628-11635
ISSN:
1520-6882
Subject:
DNA; blood glucose; blood serum; diabetes; fluorescence; fluorescent dyes; glucose; glucose oxidase; hot water treatment; humans; hydrogen peroxide; monitoring; oxidation; quantum dots
Abstract:
... As the blood glucose concentration is an important clinical parameter of diabetes, the rapid and effective detection of blood glucose is very significant for monitoring and managing diabetes. Here, a facile method to prepare Rox-DNA functionalized CdZnTeS quantum dots (QDs) was developed. The Rox-DNA functionalized CdZnTeS QDs were prepared by a one-pot hydrothermal method through phosphorothioate ...
DOI:
10.1021/acs.analchem.7b03053

7. Thermal processing effects on peanut allergen Ara h 2 allergenicity in mice and its antigenic epitope structure

Author:
Cai, Qin,   et al.   ; Zhang, Wenju; Zhu, Qingqing; Zhang, Tong; Chen, Qin;     Show all 5 Authors
Source:
Food chemistry 2016 v.212 pp. 657-662
ISSN:
0308-8146
Subject:
allergenicity; allergens; binding capacity; boiling; epitopes; frying; glycation; mice; peanuts; protein structure; roasting
Abstract:
... Ara h 2 was purified from peanuts that were thermally treated by various processes, including boiling, glycation, frying and roasting. The allergenicity of Ara h 2 in Balb/c mice and the influence of thermal processing on the structural characteristics, and binding capacity of three core antigenic epitopes were studied. The results demonstrated that boiling, glycation and frying induced the down-r ...
DOI:
10.1016/j.foodchem.2016.06.036

8. Influence of heat treatment on the structure and core IgE-binding epitopes of rAra h 2.02

Author:
Cai, Qin,   et al.   ; Zhang, Wen-ju; Zhu, Qing-qing; Chen, Qin;     Show all 4 Authors
Source:
Food chemistry 2016 v.202 pp. 404-408
ISSN:
0308-8146
Subject:
Western blotting; antibodies; atomic force microscopy; autoclaving; binding capacity; boiling; circular dichroism spectroscopy; enzyme-linked immunosorbent assay; epitopes; matrix-assisted laser desorption-ionization mass spectrometry; polyacrylamide gel electrophoresis
Abstract:
... The rAra h 2.02 was studied to determine the influence of heat treatment on its structure and core IgE-binding epitopes. The results of SDS–PAGE, Western blotting, MALDI-TOF-MS, and atomic force microscopy showed that the structure of rAra h 2.02 was altered after boiling (100°C) or autoclaving (121°C) for 20min. Furthermore, some of the protein may be aggregated. Results of circular dichroism spe ...
DOI:
10.1016/j.foodchem.2016.02.004

9. Detection of peanut (Arachis hypogaea) allergen by Real-time PCR method with internal amplification control

Author:
Cai, Qin,   et al.   ; Zhang, Wen-Ju; Guan, Xiao; Chen, Qin;     Show all 4 Authors
Source:
Food chemistry 2015 v.174 pp. 547-552
ISSN:
0308-8146
Subject:
Arachis hypogaea; DNA; DNA primers; allergens; detection limit; foods; ingredients; nucleotide sequences; peanuts; quantitative polymerase chain reaction
Abstract:
... Specific primer sets were designed based on the DNA sequence of Ara h 1, one of the major peanut (Arachis hypogaea) allergens, and a competitive internal amplification control (IAC) was designed by compound primer technology. By choosing 314copies/PCR as the IAC dosage, a Real-time PCR method with IAC was established for detecting peanut allergen Ara h 1 DNA. The method showed high specificity wit ...
DOI:
10.1016/j.foodchem.2014.11.091

10. Efficient stabilization of recombinant human coagulation factor VIII in the milk of transgenic mice using hFVIII and vWF co-expression vector transduction

Author:
Cai, Qin,   et al.   ; Ren, Xiaoye; Gong, Xiuli; Guo, Xinbing; Xu, Miao; Ren, Zhaorui; Zeng, Yitao;     Show all 7 Authors
Source:
Biotechnology letters 2015 v.37 no.6 pp. 1187-1194
ISSN:
0141-5492
Subject:
biopharmaceuticals; bioreactors; coagulation; factor VIII; gene expression; half life; heterozygosity; humans; mammary glands; mice; milk; storage conditions; temperature; transgenic animals
Abstract:
... OBJECTIVES: To investigate the reasons for the instability of human coagulation factor FVIII (hFVIII) in milk which is an intractable obstacle during the hFVIII production by a transgenic mammary gland bioreactor. RESULTS: We constructed P1A3-hFVIIIBDD and P1A3-hFVIIIBDD-IRES-vWF co-expression cassettes for generating transgenic mice. P1A3-hFVIII/CMV-vWF double heterozygotes were also prepared by ...
DOI:
10.1007/s10529-015-1793-5

11. Rhodium-Catalyzed Enantioselective Hydrogenation of Tetrasubstituted α-Acetoxy β-Enamido Esters: A New Approach to Chiral α-Hydroxyl-β-amino Acid Derivatives

Author:
Cai, Qin,   et al.   ; Wang, Qingli; Huang, Wenhua; Yuan, Haoquan; Chen, Liming; Lv, Hui; Zhang, Xumu;     Show all 7 Authors
Source:
Journal of the American Chemical Society 2014 v.136 no.46 pp. 16120-16123
ISSN:
1520-5126
Subject:
bioactive compounds; catalysts; enantiomers; esters; hydrogenation; ligands; rhodium
Abstract:
... Asymmetric hydrogenation of tetrasubtitued α-acetoxy β-enamido esters with rhodium catalysts based on chiral diphosphine ligands provides an efficient and concise route to the synthesis of chiral α-hydroxyl-β-amino acid derivatives in excellent enantioselectivities. The products are valuable chiral building blocks in many biologically active compounds and have important applications in organic syn ...
DOI:
10.1021/ja509005e

12. A New Species Record and Range Extension of two Species of Barbula in China Bryologie

Author:
Ding, Cai-Qin,   et al.   ; Bai, Sarula, Xue-Liang; Zhao, Dong-Ping; Zhang, Hong-Xia;     Show all 4 Authors
Source:
Cryptogamie 2014 v.35 no.3 pp. 327-332
ISSN:
1776-0992
Subject:
Bryopsida; flora; mosses and liverworts; photographs; China
Abstract:
... New locality records of three species of Barbula are added to the Chinese moss flora. Barbula hiroshii K. Saito is newly reported to China. Additional localities are reported for Barbula amplexifolia (Mitt.) A. Jaeg. and Barbula convoluta Hedw. Brief morphological description, range extension, digital photographs of the three species and a new key to Chinese species of Barbula are provided. ...
DOI:
10.7872/cryb.v35.iss3.2014.327

13. Treatment of the terephthalic acid-containing wastewater using a biological-aerated filter

Author:
Zhang, Cai-qin,   et al.   ; Zhang, Wen-yi; Liu, Liang; Shen, Rong-yan; Han, Xiao-jing;     Show all 5 Authors
Source:
Environmental technology 2014 v.35 no.1 pp. 70-74
ISSN:
1479-487X
Subject:
chemical oxygen demand; environmental technology; mathematical models; pH; wastewater
Abstract:
... In this paper, the biological-aerated filter (BAF) was employed to treat the wastewater containing terephthalic acid (TA). Factors that affected the efficiency of TA and COD Cᵣ removal were evaluated experimetally, including pH, hydraulic loading, hydraulic retention time (HRT) and TA volume loading. At pH 7–8, hydraulic loading rate 0.067–0.48 m ³/(m ² h), HRT more than 3.5 h and TA loading 0.04— ...
DOI:
10.1080/09593330.2013.808269

14. Antibacterial Evaluation of Novel Organoarsenic Compounds by the Microcalorimetric Method

Author:
Qin, Cai-Qin,   et al.   ; Chen, Xin-You; Hu, Xin-Liang; Xia, Cai-Fen; Liu, Yi;     Show all 5 Authors
Source:
Biological trace element research 2013 v.153 no.1-3 pp. 382-389
ISSN:
0163-4984
Subject:
Escherichia coli; antibacterial properties; arsenic; arsenicals; cell growth; inhibitory concentration 50; schiff bases
Abstract:
... Antibacterial activities of novel organoarsenic compounds As(III)-containing Schiff bases on Escherichia coli (CCTCCAB91112) were investigated by microcalorimetry in this study. The experimental result showed that the arsenic(III)-containing Schiff bases at micromolar concentration exhibit strong inhibition on the E. coli. Specifically, the growth rate constant k decreased, and the generation time ...
DOI:
10.1007/s12011-013-9660-5
PubMed:
23605049

15. Development of Polarizable Models for Molecular Mechanical Calculations. 3. Polarizable Water Models Conforming to Thole Polarization Screening Schemes

Author:
Cai, Qin,   et al.   ; Wang, Jun; Cieplak, Piotr; Hsieh, Meng-Juei; Wang, Junmei; Duan, Yong; Luo, Ray;     Show all 7 Authors
Source:
The Journal of physical chemistry 2012 v.116 no.28 pp. 7999-8008
ISSN:
1520-5207
Subject:
ambient temperature; models; physical chemistry; screening
Abstract:
... As an integrated step toward a coherent polarizable force field for biomolecular modeling, we analyzed four polarizable water models to evaluate their consistencies with the Thole polarization screening schemes utilized in our latest Amber polarizable force field. Specifically, we studied the performance of both the Thole linear and exponential schemes in these water models to assess their abiliti ...
DOI:
10.1021/jp212117d
PubMed:
22712654
PubMed Central:
PMC3493849

16. Development of Polarizable Models for Molecular Mechanical Calculations. 4. van der Waals Parametrization

Author:
Cai, Qin,   et al.   ; Wang, Junmei; Cieplak, Piotr; Li, Jie; Hsieh, Meng-Juei; Luo, Ray; Duan, Yong;     Show all 7 Authors
Source:
The Journal of physical chemistry 2012 v.116 no.24 pp. 7088-7101
ISSN:
1520-5207
Subject:
algorithms; chromosomes; energy; models; molecular dynamics; philosophy; screening; van der Waals forces; volatilization
Abstract:
... In the previous publications of this series, we presented a set of Thole induced dipole interaction models using four types of screening functions. In this work, we document our effort to refine the van der Waals parameters for the Thole polarizable models. Following the philosophy of AMBER force field development, the van der Waals (vdW) parameters were tuned for the Thole model with linear scree ...
DOI:
10.1021/jp3019759
PubMed:
22612331
PubMed Central:
PMC3391542

17. Microcalorimetric studies of the effect of cerium (Ш) on isolated rice mitochondria fed by pyruvate

Author:
Qin, Cai-Qin,   et al.   ; Xia, Cai-Fen; Jin, Jian-Cheng; Yuan, Lian; Zhao, Jie; Chen, Xin-You; Jiang, Feng-Lei; Dai, Jie; Liu, Yi;     Show all 9 Authors
Source:
Chemosphere 2013 v.91 pp. 1577-1582
ISSN:
0045-6535
Subject:
cerium; electrodes; heat; hormesis; hybrids; metabolism; mitochondria; oxygen; plant growth; pyruvic acid; rice
Abstract:
... Mitochondria were isolated from the hybrid rice Xiangzaoxian 31, then the effects of low and high concentrations of Ce (Ш) on metabolism of mitochondria fed by pyruvate were investigated respectively, by microcalorimetry and oxygen electrode method The thermogenic curve of mitochondria without Ce (Ш) could be divided into three parts: activity recovery phase, stationary phase and decline phase. An ...
DOI:
10.1016/j.chemosphere.2012.12.049
PubMed:
23352518

18. Ce(III)-Induced Rice Mitochondrial Permeability Transition Investigated by Spectroscopic and Microscopic Studies

Author:
Qin, Cai-Qin,   et al.   ; Xia, Cai-Fen; Zhao, Jie; Jin, Jian-Cheng; Yuan, Lian; Chen, Xin-You; Peng, Wu; Jiang, Feng-Lei; Dai, Jie; Liu, Yi;     Show all 10 Authors
Source:
Biological trace element research 2013 v.152 no.2 pp. 284-291
ISSN:
0163-4984
Subject:
cerium; crops; feed additives; fertilizers; food chain; human health; membrane fluidity; membrane potential; membrane proteins; mitochondria; permeability; rice; spectroscopy; thiols; ultrastructure
Abstract:
... Cerium has been widely used as fertilizer and feed additives in agriculture, but it might finally impair human health by food chain accumulation with its dosage increased in environmental and crops samples. To resolve the conflict, we investigated the effects of Ce(III) on isolated rice mitochondrial permeability transition (MPT) by examining mitochondrial swelling, transmembrane potential, membra ...
DOI:
10.1007/s12011-013-9621-z
PubMed:
23417494

19. Composition and phylogenetic analysis of wheat cryptochrome gene family

Author:
Zhang, Cai Qin,   et al.   ; Xu, Pei; Zhu, Hui Lan; Xu, Hai Bin; Zhang, Zheng Zhi; Zhang, Li Xia; Ma, Zheng Qiang;     Show all 7 Authors
Source:
Molecular biology reports 2010 v.37 no.2 pp. 825-832
ISSN:
0301-4851
Subject:
Southern blotting; photoreceptors; chromosomes; cryptochromes; Magnoliopsida; phylogeny; expressed sequence tags; polymerase chain reaction; wheat; blue light; Liliopsida; databases
Abstract:
... Cryptochrome (CRY) gene family encodes photoreceptors mediating developmental responses to blue light throughout the life of plants. We report here the characterization of CRY gene family in hexaploid wheat. Degenerate PCR amplification of the regions encoding the conserved flavin-binding domain of CRY proteins yielded seven bands, resulting from amplification of CRY1a, CRY1b and CRY2 homologous g ...
DOI:
10.1007/s11033-009-9628-x
PubMed:
19626459

20. Selection of reliable reference genes for expression studies by reverse transcription quantitative real-time PCR in litchi under different experimental conditions

Author:
Li, Cai-Qin,   et al.   ; Zhong, Hai-Ying; Chen, Jian-Wen; Chen, Lei; Wu, Jian-Yang; Chen, Jian-Ye; Lu, Wang-Jin; Li, Jian-Guo;     Show all 8 Authors
Source:
Plant cell reports 2011 v.30 no.4 pp. 641-653
ISSN:
0721-7714
Subject:
RNA; actin; defoliation; developmental stages; fruit trees; gene expression; genes; girdling; glyceraldehyde-3-phosphate dehydrogenase; guidelines; naphthaleneacetic acid; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; reverse transcription; shade; tissues; transcription factors; tubulin; ubiquitin
Abstract:
... Reverse transcription quantitative real-time PCR (RT-qPCR), a sensitive technique for quantifying gene expression, depends on the stability of the reference gene(s) used for data normalization. Only a few studies on reference genes have been done in fruit trees and none in litchi. In the present study, seven frequently used candidate reference genes, including actin (ACTIN), glyceraldehyde-3-phosp ...
DOI:
10.1007/s00299-010-0992-8