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Marcus Vinícius Merfa; Eduarda Regina Fischer; Mariana de Souza e Silva; Carolina Sardinha Francisco; Helvécio Della Coletta-Filho; Alessandra Alves de Souza
... Huanglongbing (HLB) is currently the most devastating disease of citrus worldwide. Both bacteria ‘Candidatus Liberibacter asiaticus’ (CLas) and ‘Candidatus Liberibacter americanus’ (CLam) are associated with HLB in Brazil but with a strong prevalence of CLas over CLam. Conventionally, HLB management focuses on controlling the insect vector population (Diaphorina citri; also known as Asian citrus p ...
CandidatusLiberibacter asiaticus, etc ; Citrus; DNA; ethidium; greening disease; propidium; quantitative polymerase chain reaction; Show all 7 Subjects
Abstract:
... Citrus Huanglongbing (HLB, also known as “citrus greening”), an important disease worldwide, is associated with three species of phloem-limited Candidatus liberibacter, of which Candidatus L. asiaticus (CLas) is the predominant one that has severely affected citrus production. TaqMan real-time polymerase chain reaction (PCR) (TM) has been the standard and very efficient method to diagnose several ...
CandidatusLiberibacter solanacearum, etc ; Apiaceae; Bactericera; DNA; Solanaceae; abdomen; bacteria; cross contamination; ethanol; head; insects; Show all 11 Subjects
Abstract:
... ‘Candidatus Liberibacter solanacearum’ (CaLsol) is an uncultured bacterium, transmitted by psyllids and associated with several diseases in Solanaceae and Apiaceae crops. CaLsol detection in psyllids often requires insect destruction, preventing a subsequent morphological identification. In this work, we have assessed the influence on the detection of CaLsol by PCR in Bactericera trigonica (Hemipt ...
... Trioza erytreae is one natural psyllid vector of Candidatus liberibacter, the causal agent of the citrus greening disease (HLB). Since its introduction in 2014 into the Iberian Peninsula, T. erytreae was able to spread continuously toward southern coastal regions of Portugal and northern coastal regions of the Cantabric sea in Spain. Identifying key areas of potential colonization by the psyllid v ...
CandidatusLiberibacter asiaticus, etc ; Bergera koenigii; Citrus reticulata; Diaphorina citri; entomology; insects; instars; oviposition; pathogens; phylogeny; Show all 10 Subjects
Abstract:
... The black curry leaf psyllid, Diaphorina communis, is a host of the citrus pathogen, ‘Candidatus Liberibacter asiaticus’ (‘CLas’). However, there is a paucity of information on its biology; hence, this study was conducted to evaluate survival and development on citrus, in this instance mandarin (Citrus reticulata) and curry leaf (Bergera koenigii), and transmission of ‘CLas’. Given its similarity ...
... Huanglongbing is caused by Candidatus Liberibacter asiaticus (CLas) and transmitted by Diaphorina citri. D. citri harbors various insect-specific viruses, including the Diaphorina citri flavi-like virus (DcFLV). The distribution and biological role of DcFLV in its host and the relationship with CLas are unknown. DcFLV was found in various organs of D. citri, including the midgut and salivary gland ...
... “Candidatus Liberibacter solanacearum” (Lso), transmitted by the potato psyllid (Bactericera cockerelli), is the causal agent of potato zebra chip, but can also infect other solanaceous plants, including peppers. Studies were conducted to investigate whether Lso could be transmitted to the next generation of plants through seeds from infected pepper plants. In 2014, jalapeno pepper plants were inf ...
... Huanglongbing (HLB) is considered one of the most important diseases in citrus industry, which currently has no cure. In Argentina, ‘Candidatus Liberibacter asiaticus’ (CLas) has been detected only in plant material. It is transmitted by Diaphorina citri, which is distributed in most of the country’s citrus regions. The aim of this study was to detect the presence of CLas in D. citri in Argentina. ...
... Candidatus Liberibacter spp. are fastidious α-proteobacteria that cause multiple diseases on plant hosts of economic importance, including the most devastating citrus disease: Huanglongbing (HLB). HLB was reported in Asia a century ago but has since spread worldwide. Understanding the pathogenesis of Candidatus Liberibacter spp. remains challenging as they are yet to be cultured in artificial medi ...
... The pathogens associated with citrus Huanglongbing symptoms, including yellowing and mottled leaves in Citrus maxima, an important economic crop on Hainan Island of China, were identified and characterized. In the study, detection, genetic variation and phylogenetic relationship analysis of the pathogens were performed based on 16S rRNA and β-operon gene fragments specific to phytoplasma and Candi ...
CandidatusLiberibacter asiaticus, etc ; Citrus; Citrus tristeza virus; Diaphorina citri; genome; greening disease; industry; insects; integrated pest management; Florida; Show all 10 Subjects
Abstract:
... The destructive citrus disease, Huanglongbing (HLB) or citrus greening, continues to devastate Florida’s citrus industry. A hemipteran insect, the Asian citrus psyllid (ACP), disperses Candidatus Liberibacter asiaticus, one of the putative bacterial pathogens of HLB. This study builds upon ongoing research utilizing high-throughput sequencing to analyze the virome of ACP populations collected from ...
... The phytopathogenic bacteria Candidatus Liberibacter asiaticus (CLas) is the causal agent of huanglongbing (HLB), a destructive disease affecting citrus worldwide. It is vectored by the Asian citrus psyllid Diaphorina citri, an invasive pest that has spread across the Caribbean Islands and Florida over the last 20 years. It has been suggested that intercropping citrus and guava could significantly ...
CandidatusLiberibacter solanacearum, etc ; Bactericera cockerelli; Solanaceae; Tamarixia triozae; Triozidae; bacteria; domestication; olfactometers; parasitism; parasitoids; pest control; potatoes; tomatoes; Show all 13 Subjects
Abstract:
... The potato/tomato psyllid Bactericera cockerelli is the Candidatus Liberibacter solanacearum bacterium vector that causes diseases in Solanaceae crops. Pest control is based on synthetic chemical insecticides, plant extracts, and natural enemies such as parasitoids. Tamarixia triozae feeds on nymphs of B. cockerelli, reaching up to 95% parasitism. This work aimed to evaluate the parasitic performa ...
CandidatusLiberibacter asiaticus, etc ; Citrus sinensis; apigenin; electrospray ionization mass spectrometry; gene expression; greening disease; metabolites; naringin; p-coumaric acid; Show all 9 Subjects
Abstract:
... Huanglongbing (HLB), a devastating disease for citrus worldwide, is caused by Candidatus Liberibacter asiaticus (CLas). In this study, we employed a novel extractive electrospray ionization-mass spectrometry (EESI-MS) method to analyze the metabolites in leaves of uninfected and HLB-infected Newhall navel orange. The results showed that uninfected and HLB-infected leaves could be readily distingui ...
... Phytopathogen infections not only affect the physiology of host plants but also the preference of insect vectors; these modifications may increase the spread of infection. For this, we determined the effects of “Candidatus Liberibacter asiaticus” (CLas) infection on the preference of an insect vector (Diaphorina citri) for its uninfected or CLas-infected host (Citrus sinensis) and found that the i ...
... Citriculture landscapes in the U.S. are typically habitat mosaics of commercial groves interspersed with residential areas supporting a variety of unmanaged citrus. Diaphorina citri the vector of Candidatus Liberibacter asiaticus, the causal agent of Huanglongbing feeds on citrus in both habitats. We postulated that residential citrus function as a ‘source’ of D. citri that infest groves, function ...
... Huanglongbing, or citrus greening disease, is the most serious disease of citrus worldwide and is associated with plant infection by ‘Candidatus Liberibacter asiaticus’ (CLas) and other Liberibacter species. CLas is transmitted by Diaphorina citri, the Asian citrus psyllid, in a circulative propagative manner. Circulative propagative transmission is a complex process comprising at least three step ...
... Crop diseases may be affected by landscape composition, but limited quantitative information is available. We studied the effects of landscape factors on the incidence of the psyllid-transmitted bacterium Candidatus Liberibacter solanacearum (CLso) haplotype C in carrots in Finland. Samples were collected from 104 carrot fields in 2013 and 2014. The relationship between CLso incidence and landscap ...
CandidatusLiberibacter asiaticus, etc ; Citrus; canopy; dieback; greening disease; longevity; plant pathology; plant response; root growth; root systems; spring; summer; tree age; Show all 13 Subjects
Abstract:
... Huanglongbing (HLB), caused by ‘Candidatus Liberibacter asiaticus’ (CLas), is a devastating disease of citrus. After initial infection, CLas quickly colonizes the root system before canopy symptoms develop. There is limited understanding of CLas movement from roots to canopy and local and systemic effects on root dynamics. Using split-root rhizoboxes and late summer below-the-split bud inoculation ...
CandidatusLiberibacter solanacearum, etc ; Bactericera maculipennis; Fraxinus mandshurica; Fraxinus nigra; Nearctic region; death; dieback; insects; plant pathology; provenance; recombinant DNA; Saskatchewan; United Kingdom; Show all 13 Subjects
Abstract:
... ‘Candidatus Liberibacter’ species are associated with severe, economically important diseases. Nearly all known species are putatively insect transmitted, specifically by psyllids. Detection of ‘Ca. Liberibacter’ in plants is complicated by their uneven distribution in host plants and largely fastidius nature. The death of black (Fraxinus nigra) and mancana (Fraxinus mandshurica) ash trees in Sask ...