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CandidatusLiberibacterasiaticus, etc ; Citrus; DNA; ethidium; greening disease; propidium; quantitative polymerase chain reaction; Show all 7 Subjects
Abstract:
... Citrus Huanglongbing (HLB, also known as “citrus greening”), an important disease worldwide, is associated with three species of phloem-limited Candidatus liberibacter, of which Candidatus L. asiaticus (CLas) is the predominant one that has severely affected citrus production. TaqMan real-time polymerase chain reaction (PCR) (TM) has been the standard and very efficient method to diagnose several ...
CandidatusLiberibacterasiaticus, etc ; Bergera koenigii; Citrus reticulata; Diaphorina citri; entomology; insects; instars; oviposition; pathogens; phylogeny; Show all 10 Subjects
Abstract:
... The black curry leaf psyllid, Diaphorina communis, is a host of the citrus pathogen, ‘Candidatus Liberibacter asiaticus’ (‘CLas’). However, there is a paucity of information on its biology; hence, this study was conducted to evaluate survival and development on citrus, in this instance mandarin (Citrus reticulata) and curry leaf (Bergera koenigii), and transmission of ‘CLas’. Given its similarity ...
CandidatusLiberibacterasiaticus, etc ; Citrus macrophylla; Diaphorina citri; females; grapefruits; greenhouses; greening disease; midgut; progeny; virology; viruses; Show all 11 Subjects
Abstract:
... Huanglongbing is caused by Candidatus Liberibacter asiaticus (CLas) and transmitted by Diaphorina citri. D. citri harbors various insect-specific viruses, including the Diaphorina citri flavi-like virus (DcFLV). The distribution and biological role of DcFLV in its host and the relationship with CLas are unknown. DcFLV was found in various organs of D. citri, including the midgut and salivary gland ...
... Huanglongbing (HLB) is considered one of the most important diseases in citrus industry, which currently has no cure. In Argentina, ‘Candidatus Liberibacter asiaticus’ (CLas) has been detected only in plant material. It is transmitted by Diaphorina citri, which is distributed in most of the country’s citrus regions. The aim of this study was to detect the presence of CLas in D. citri in Argentina. ...
... The pathogens associated with citrus Huanglongbing symptoms, including yellowing and mottled leaves in Citrus maxima, an important economic crop on Hainan Island of China, were identified and characterized. In the study, detection, genetic variation and phylogenetic relationship analysis of the pathogens were performed based on 16S rRNA and β-operon gene fragments specific to phytoplasma and Candi ...
CandidatusLiberibacterasiaticus, etc ; Citrus; Citrus tristeza virus; Diaphorina citri; genome; greening disease; industry; insects; integrated pest management; Florida; Show all 10 Subjects
Abstract:
... The destructive citrus disease, Huanglongbing (HLB) or citrus greening, continues to devastate Florida’s citrus industry. A hemipteran insect, the Asian citrus psyllid (ACP), disperses Candidatus Liberibacter asiaticus, one of the putative bacterial pathogens of HLB. This study builds upon ongoing research utilizing high-throughput sequencing to analyze the virome of ACP populations collected from ...
... The phytopathogenic bacteria Candidatus Liberibacter asiaticus (CLas) is the causal agent of huanglongbing (HLB), a destructive disease affecting citrus worldwide. It is vectored by the Asian citrus psyllid Diaphorina citri, an invasive pest that has spread across the Caribbean Islands and Florida over the last 20 years. It has been suggested that intercropping citrus and guava could significantly ...
CandidatusLiberibacterasiaticus, etc ; Citrus sinensis; apigenin; electrospray ionization mass spectrometry; gene expression; greening disease; metabolites; naringin; p-coumaric acid; Show all 9 Subjects
Abstract:
... Huanglongbing (HLB), a devastating disease for citrus worldwide, is caused by Candidatus Liberibacter asiaticus (CLas). In this study, we employed a novel extractive electrospray ionization-mass spectrometry (EESI-MS) method to analyze the metabolites in leaves of uninfected and HLB-infected Newhall navel orange. The results showed that uninfected and HLB-infected leaves could be readily distingui ...
... Phytopathogen infections not only affect the physiology of host plants but also the preference of insect vectors; these modifications may increase the spread of infection. For this, we determined the effects of “Candidatus Liberibacter asiaticus” (CLas) infection on the preference of an insect vector (Diaphorina citri) for its uninfected or CLas-infected host (Citrus sinensis) and found that the i ...
... Citriculture landscapes in the U.S. are typically habitat mosaics of commercial groves interspersed with residential areas supporting a variety of unmanaged citrus. Diaphorina citri the vector of Candidatus Liberibacter asiaticus, the causal agent of Huanglongbing feeds on citrus in both habitats. We postulated that residential citrus function as a ‘source’ of D. citri that infest groves, function ...
... Huanglongbing, or citrus greening disease, is the most serious disease of citrus worldwide and is associated with plant infection by ‘Candidatus Liberibacter asiaticus’ (CLas) and other Liberibacter species. CLas is transmitted by Diaphorina citri, the Asian citrus psyllid, in a circulative propagative manner. Circulative propagative transmission is a complex process comprising at least three step ...
CandidatusLiberibacterasiaticus, etc ; Citrus; canopy; dieback; greening disease; longevity; plant pathology; plant response; root growth; root systems; spring; summer; tree age; Show all 13 Subjects
Abstract:
... Huanglongbing (HLB), caused by ‘Candidatus Liberibacter asiaticus’ (CLas), is a devastating disease of citrus. After initial infection, CLas quickly colonizes the root system before canopy symptoms develop. There is limited understanding of CLas movement from roots to canopy and local and systemic effects on root dynamics. Using split-root rhizoboxes and late summer below-the-split bud inoculation ...
... Although the mitochondria retain all required enzymes for an intact tricarboxylic acid (TCA) cycle, plants might shift the cyclic flux from the TCA cycle to an alternative noncyclic pathway via γ-aminobutyric acid (GABA) shunt under specific physiological conditions. We hypothesize that several genes may ease this noncyclic flux and contribute to the citrus response to the phytopathogenic bacteriu ...
CandidatusLiberibacterasiaticus, etc ; Citrus; Cuscuta campestris; Diaphorina citri; case studies; greening disease; mitochondria; mitochondrial genome; phylogeny; tandem repeat sequences; transposons; China; Yangtze River; Show all 13 Subjects
Abstract:
... Citrus huanglongbing (HLB) is present in 10 provinces in China and is associated with ‘Candidatus Liberibacter asiaticus’ (CLas), which is transmitted by the Asian citrus psyllid (Diaphorina citri, ACP). To date, HLB and ACP have expanded to Yibin city of Sichuan Province, posing an imminent threat to the citrus belt of the upper and middle reaches of the Yangtze River, an important late-maturing ...
CandidatusLiberibacterasiaticus, etc ; Citrus; Diaphorina citri; Enterobacteriaceae; Wolbachia; digestive system; endosymbionts; entomology; greening disease; immunity; industry; insect vectors; microbiome; multidimensional scaling; pathogens; pests; species diversity; Show all 17 Subjects
Abstract:
... The Asian citrus psyllid (Diaphorina citri) is a major pest of the citrus industry and is also the vector for Candidatus Liberibacter asiaticus (CLas), a destructive Huanglongbing (HLB) disease of citrus trees. Insect endosymbionts and gut bacteria play important roles in vector-pathogen interactions and host immunity. Thus, our aim was to evaluate the correlation between CLas infection and the mi ...
CandidatusLiberibacterasiaticus, etc ; Citrus; Diaphorina citri; Psyllidae; bacteria; canopy; citrus canker; greening disease; horticulture; insecticides; integrated pest management; leafminers; microclimate; plant protection; sooty molds; tree health; trees; Florida; Show all 18 Subjects
Abstract:
... Following the discovery of Asian citrus psyllid (ACP) in 1998 and Huanglongbing (HLB) in 2005, this destructive disease associated with the phloem-limited bacterium Candidatus Liberibacter asiaticus (CLas) now affects most mature citrus trees in Florida. Despite the endemic presence of ACP and HLB, Florida citrus growers continue to plant new trees. The young trees are more vulnerable to both ACP ...
Marcus Vinícius Merfa; Eduarda Regina Fischer; Mariana de Souza e Silva; Carolina Sardinha Francisco; Helvécio Della Coletta-Filho; Alessandra Alves de Souza
... Huanglongbing (HLB) is currently the most devastating disease of citrus worldwide. Both bacteria ‘Candidatus Liberibacter asiaticus’ (CLas) and ‘Candidatus Liberibacter americanus’ (CLam) are associated with HLB in Brazil but with a strong prevalence of CLas over CLam. Conventionally, HLB management focuses on controlling the insect vector population (Diaphorina citri; also known as Asian citrus p ...
CandidatusLiberibacterasiaticus, etc ; Citrus; Diaphorina citri; RNA interference; body weight; chitin; dose response; double-stranded RNA; genes; greening disease; hexokinase; insect development; messenger RNA; mortality; pest control; pests; Show all 16 Subjects
Abstract:
... BACKGROUND: Silencing specific genes in pests using RNA interference (RNAi) technology is a promising new pest‐control strategy. The Asian citrus psyllid, Diaphorina citri Kuwayama, is the most important citrus pest because it transmits Candidatus Liberibacter asiaticus, which causes huanglongbing. Chitin is essential for insect development, and enzymes in this pathway are attractive targets for p ...
... ‘Shatangju’ mandarin (Citrus reticulate Blanco cv. Shatangju) is a Chinese citrus specialty in southern China with a delicious taste and an attractive appearance. Huanglongbing (HLB) caused by Candidatus Liberibacter asiaticus (CLas) threatens the Shatangju industry seriously. Fruits from citrus trees with HLB show ‘red nose’ peels with a serious reduction in fruit value. Differentially expressed ...
... The Asian citrus psyllid, Diaphorina citri, is the vector of Candidatus Liberibacter asiaticus (CLas), the presumed causative agent of citrus greening disease. For successful transmission, CLas must cross the gut barrier, requiring interaction with proteins on the midgut epithelium. We compared the relative abundance of gut surface proteins for both adult and nymph D. citri, as nymphs are particul ...