PubAg

Main content area

You searched for: Author "Cheng, Xiyao" Remove constraint Author: "Cheng, Xiyao"
Start Over

Search

Did you mean to type: xiyan or xinyao?

4  Search Results

1 - 4 of 4
Number of results to display per page

Search Results

1. Structure-based reconstruction of a Mycobacterium hypothetical protein into an active Δ5–3-ketosteroid isomerase

Author:
Cheng, Xiyao,   et al.   ; Peng, Fei; Wang, Hongwei; Song, Shikui; Chen, Tian; Li, Xin; He, Yijun; Huang, Yongqi; Liu, Sen; Yang, Fei; Su, Zhengding;     Show all 11 Authors
Source:
Biochimica et biophysica acta 2019 v.1867 no.9 pp. 821-830
ISSN:
1570-9639
Subject:
Mycobacterium tuberculosis; Pseudomonas putida; X-ray diffraction; active sites; amino acid sequences; engineering; hydrogen bonding; isomerases; models; proteins; sequence analysis; site-directed mutagenesis
Abstract:
... Protein engineering based on structure homology holds the potential to engineer steroid-transforming enzymes on demand. Based on the genome sequencing analysis of industrial Mycobacterium strain HGMS2 to produce 4-androstene-3,17-dione (4-AD), three hypothetical proteins were predicted as putative Δ5–3-ketosteroid isomerases (KSIs) to catalyze an intramolecular proton transfer involving the transf ...
DOI:
10.1016/j.bbapap.2019.06.008

2. Recombinant Butelase-Mediated Cyclization of the p53-Binding Domain of the Oncoprotein MdmX-Stabilized Protein Conformation as a Promising Model for Structural Investigation

Author:
Cheng, Xiyao,   et al.   ; Pi, Ni; Gao, Meng; Liu, Huili; Kuang, Zhengkun; Yang, Zixin; Yang, Jing; Zhang, Bailing; Chen, Yao; Liu, Sen; Huang, Yongqi; Su, Zhengding;     Show all 12 Authors
Source:
Biochemistry 2019 v.58 no.27 pp. 3005-3015
ISSN:
1520-4995
Subject:
Komagataella pastoris; antineoplastic agents; crystallization; ligands; ligases; melting; melting point; mice; models; nuclear magnetic resonance spectroscopy; oncogene proteins; polypeptides; protein conformation; sortase A; yeasts
Abstract:
... Cyclization of the polypeptide backbone has proven to be a powerful strategy for enhancing protein stability for fundamental research and pharmaceutical application. The use of such an approach is restricted by how well a targeted polypeptide can be efficiently ligated. Recently, an Asx-specific peptide ligase identified from a tropical cyclotide-producing plant and named butelase 1 exhibited exce ...
DOI:
10.1021/acs.biochem.9b00263

3. Effect of the Flexible Regions of the Oncoprotein Mouse Double Minute X on Inhibitor Binding Affinity

Author:
Cheng, Xiyao,   et al.   ; Qin, Lingyun; Liu, Huili; Chen, Rong; Zhou, Jingjing; Chen, Yao; Huang, Yongqi; Su, Zhengding;     Show all 8 Authors
Source:
Biochemistry 2017 v.56 no.44 pp. 5943-5954
ISSN:
1520-4995
Subject:
amino acid sequences; binding capacity; chemical interactions; drugs; engineering; ligands; mice; models; molecular dynamics; mutants; oncogene proteins; pharmacology; prediction; protein conformation; protein engineering; screening
Abstract:
... The oncoprotein MdmX (mouse double minute X) is highly homologous to Mdm2 (mouse double minute 2) in terms of their amino acid sequences and three-dimensional conformations, but Mdm2 inhibitors exhibit very weak affinity for MdmX, providing an excellent model for exploring how protein conformation distinguishes and alters inhibitor binding. The intrinsic conformation flexibility of proteins plays ...
DOI:
10.1021/acs.biochem.7b00903

4. A novel strategy to prepare the precursor peptide of liraglutide

Author:
Cheng, Xiyao,   et al.   ; Cheng, Nian; Yang, Lei; Dai, Ning; Hu, Zhongping; Yang, Fan; Chen, Rong; Zhou, Jingjing; Huang, Yongqi; Su, Zhengding;     Show all 10 Authors
Source:
Process biochemistry 2017 v.62 pp. 10-15
ISSN:
1359-5113
Subject:
Escherichia coli; formic acid; glucagon-like peptide 1; glycemic effect; humans; ion exchange chromatography; mutants; palmitic acid; protein secretion; protein synthesis; reversed-phase liquid chromatography; secretin; solvents; yeasts
Abstract:
... Glucagon-like peptide-1 (GLP-1) is a natural incretin hormone released from intestinal L-cells in response to hormonal, neural and nutrient stimuli. Liraglutide is derived from human GLP-1 by substituting Lys34 with Arg and conjugating a palmitic acid to Lys26 via a glutamate spacer, which has a long-lasting hypoglycemic effect. The precursor of liraglutide is a K34R mutant of GLP-1 (GLP-1ᴿ³⁴) and ...
DOI:
10.1016/j.procbio.2017.07.006