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1. Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii

Author:
Cheng, Xun-jia,   et al.   ; Wu, Duo; Feng, Meng; Wang, Zhi-xin; Qiao, Ke; Tachibana, Hiroshi;     Show all 6 Authors
Source:
Parasites & vectors 2018 v.11 no.1 pp. 604
ISSN:
1756-3305
Subject:
Acanthamoeba castellanii; Escherichia coli; biochemical pathways; biosynthesis; catalytic activity; confocal laser scanning microscopy; cysteine; cysteine synthase; dose response; drugs; encephalitis; energy metabolism; enzyme activity; gene expression; genes; human diseases; isoelectric point; keratitis; mice; molecular weight; phosphoglycerate dehydrogenase; polyclonal antibodies; quantitative polymerase chain reaction; recombinant proteins; serine; sodium sulfide
Abstract:
... BACKGROUND: Acanthamoeba spp. can cause serious human infections, including Acanthamoeba keratitis, granulomatous amoebic encephalitis and cutaneous acanthamoebiasis. Cysteine biosynthesis and the L-serine metabolic pathway play important roles in the energy metabolism of Acanthamoeba spp. However, no study has confirmed the functions of cysteine synthase (AcCS) in the cysteine pathway and phospho ...
DOI:
10.1186/s13071-018-3188-7

2. Screening for biomarkers reflecting the progression of Babesia microti infection

Author:
Cheng, Xun-Jia,   et al.   ; Xu, Bin; Liu, Xiu-Feng; Cai, Yu-Chun; Huang, Ji-Lei; Zhang, Rui-Xiang; Chen, Jun-Hu; Zhou, Xia; Xu, Xue-Nian; Zhou, Yan; Zhang, Ting; Chen, Shen-Bo; Li, Jian; Wu, Qun-Feng; Sun, Cheng-Song; Fu, Yong-Feng; Chen, Jia-Xu; Zhou, Xiao-Nong; Hu, Wei;     Show all 19 Authors
Source:
Parasites & vectors 2018 v.11 no.1 pp. 379
ISSN:
1756-3305
Subject:
Babesia microti; Escherichia coli; Western blotting; animal models; antibodies; antibody detection; antigens; babesiosis; biomarkers; cell free system; disease course; enzyme-linked immunosorbent assay; erythrocytes; humans; immunoglobulin G; immunoglobulin M; mass spectrometry; mice; microarray technology; microscopy; parasitemia; parasites; patients; polymerase chain reaction; public health; recombinant proteins; screening; two-dimensional gel electrophoresis; vaccination; vaccine development; wheat germ
Abstract:
... BACKGROUND: Babesiosis is caused by the invasion of erythrocytes by parasites of the Babesia spp. Babesia microti is one of the primary causative agents of human babesiosis. To better understand the status of the disease, discovering key biomarkers of the different infection stages is crucial. RESULTS: This study investigated B. microti infection in the mouse model from 0 to 270 days post-infectio ...
DOI:
10.1186/s13071-018-2951-0
PubMed:
29970143
PubMed Central:
PMC6029176

3. Comparison of serine-rich protein genes of Entamoeba histolytica isolates obtained from institutions for the mentally retarded in Kanagawa and Shizuoka Prefectures, Japan

Author:
Cheng, Xun-Jia,   et al.   ; Fu, Yong-Feng; Nagakura, Kouichi; Tachibana, Hiroshi;     Show all 4 Authors
Source:
Parasitology research 2010 v.107 no.4 pp. 999-1002
ISSN:
0932-0113
Subject:
Entamoeba histolytica; amebiasis; genes; men; retrospective studies; Japan
Abstract:
... In Japan, amebiasis has been observed in homosexual men, in institutionalized persons, and in overseas travelers. We have previously reported an outbreak of amebiasis that occurred from 1986 to 1994 in institutions for the mentally retarded in Kanagawa and Shizuoka Prefectures in Eastern Japan. Entamoeba histolytica but not Entamoeba dispar was identified in Entamoeba cultures obtained from cyst p ...
DOI:
10.1007/s00436-010-1954-3
PubMed:
20556425

4. Characterization of Entamoeba histolytica Intermediate Subunit Lectin-Specific Human Monoclonal Antibodies Generated in Transgenic Mice Expressing Human Immunoglobulin Loci

Author:
Cheng, Xun-Jia,   et al.   ; Tachibana, Hiroshi; Tsukamoto, Hideo; Itoh, Johbu;     Show all 4 Authors
Source:
Infection and immunity 2009 v.77 no.1 pp. 549-556
ISSN:
0019-9567
Subject:
Entamoeba histolytica; abscess; amebiasis; epitopes; flow cytometry; genes; humans; immunoglobulin G; immunoglobulin M; lectins; loci; mice; microscopy; monoclonal antibodies; trophozoites; vacuoles
Abstract:
... Four fully human monoclonal antibodies (MAbs) to Entamoeba histolytica intermediate subunit lectin (Igl) were prepared in XenoMouse mice, which are transgenic mice expressing human immunoglobulin loci. Examination of the reactivities of these MAbs to recombinant Igl1 and Igl2 of E. histolytica showed that XEhI-20 {immunoglobulin G2(κ) [IgG2(κ)]} and XEhI-28 [IgG2(κ)] were specific to Igl1, XEhI-B5 ...
DOI:
10.1128/IAI.01002-08
PubMed:
19001071
PubMed Central:
PMC2612286

5. Generation of a Neutralizing Human Monoclonal Antibody Fab Fragment to Surface Antigen 1 of Toxoplasma gondii Tachyzoites

Author:
Cheng, Xun-Jia,   et al.   ; Fu, Yong-Feng; Feng, Meng; Ohnishi, Kenji; Kimura, Tamon; Itoh, Johbu; Tachibana, Hiroshi;     Show all 7 Authors
Source:
Infection and immunity 2011 v.79 no.1 pp. 512-517
ISSN:
0019-9567
Subject:
DNA libraries; Toxoplasma gondii; antigens; clones; dissociation; germ cells; humans; immunization; immunoglobulin genes; lymphocytes; mice; microscopy; monoclonal antibodies; mortality; neutralization; patients; sequence analysis; tachyzoites; toxoplasmosis
Abstract:
... A combinatorial immunoglobulin gene library was constructed from lymphocytes in peripheral blood of a patient with toxoplasmosis and screened for production of human monoclonal antibody Fab fragments to recombinant surface antigen 1 (SAG1) of Toxoplasma gondii. Two Fab clones, Tox203 and Tox1403, which consisted of a common heavy chain and different light chains, showed positive staining on the en ...
DOI:
10.1128/IAI.00969-10
PubMed:
20956568
PubMed Central:
PMC3019919

6. Modification of a human monoclonal antibody Fab fragment specific for Plasmodium falciparum 19-kDa C-terminal merozoite surface protein 1 by site-directed mutagenesis

Author:
Cheng, Xun-Jia,   et al.   ; Tao, Yan-Lin; Fu, Yong-Feng; Tsukamoto, Hideo; Yoshihara, Eisaku; Tachibana, Hiroshi;     Show all 6 Authors
Source:
Parasitology research 2008 v.103 no.2 pp. 429-433
ISSN:
0932-0113
Subject:
Plasmodium falciparum; antigen-antibody reactions; humans; merozoites; polymerase chain reaction; site-directed mutagenesis; surface proteins
Abstract:
... We recently produced human monoclonal antibody Fab fragments specific for the 19-kDa C-terminal merozoite surface protein 1 of Plasmodium falciparum in a bacterial expression system. The effect of single amino acid modifications in the third complementarity-determining regions of the heavy and light chains on affinity was examined in one of the Fab fragments, Pf25. Recombination polymerase chain r ...
DOI:
10.1007/s00436-008-0994-4
PubMed:
18446371

7. Production of High-Affinity Human Monoclonal Antibody Fab Fragments to the 19-Kilodalton C-Terminal Merozoite Surface Protein 1 of Plasmodium falciparum

Author:
Cheng, Xun-Jia,   et al.   ; Hayasaka, Hitoshi; Watanabe, Katsuomi; Tao, Yan-Lin; Liu, Jin-Ye; Tsukamoto, Hideo; Horii, Toshihiro; Tanabe, Kazuyuki; Tachibana, Hiroshi;     Show all 9 Authors
Source:
Infection and immunity 2007 v.75 no.7 pp. 3614-3620
ISSN:
0019-9567
Subject:
DNA libraries; Plasmodium falciparum; antibody affinity; clones; dissociation; epitopes; erythrocytes; fluorescence microscopy; germ cells; humans; immunoglobulin genes; lymphocytes; malaria; merozoites; mice; monoclonal antibodies; patients; schizonts; sequence analysis; surface proteins; trophozoites
Abstract:
... A combinatorial immunoglobulin gene library was constructed from peripheral blood lymphocytes of eight patients infected with Plasmodium falciparum and was screened for the production of human monoclonal antibody Fab fragments to the C-terminal 19-kDa fragment of P. falciparum merozoite surface protein 1 (MSP-1₁₉). Three Fab clones recognized recombinant MSP-1₁₉ under nonreducing conditions. Indir ...
DOI:
10.1128/IAI.00062-07
PubMed:
17452466
PubMed Central:
PMC1932930