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... A commercial “concentrating pipette” was scrutinized from the perspective of sample volume reduction to augment native bacterial quantitation and identification within food samples. Aspects related to the elution protocol were evaluated for their impact on bacteria recovery. The results demonstrated there was no significant effect from different diluents/eluents on the relative recovery rate. Furt ...
Chin-YiChen, et al. ; Elizabeth A. McMillan; Ly-Huong T. Nguyen; Lari M. Hiott; Poonam Sharma; Charlene R. Jackson; Jonathan G. Frye; Show all 7 Authors
... Salmonella enterica and Escherichia coli are important human pathogens that frequently contain plasmids, both large and small, carrying antibiotic resistance genes. Large conjugative plasmids are known to mobilize small Col plasmids, but less is known about the specificity of mobilization. In the current study, six S. enterica and four E. coli strains containing large plasmids were tested for thei ...
antibiotic resistance; data collection; genomics; kanamycin kinase; liquid chromatography; mass spectrometry; monitoring; peptides; proteomics; rapid methods; spectral analysis; spectrometers
Abstract:
... Improvements in mass spectrometry technology to include instrument duty cycle, resolution, and sensitivity suggest mass spectrometry as a highly competitive alternative to conventional microbiological proteomic techniques. Targeted mass spectral analysis, sans prior empirical measurements, has begun to solely use the enormous amount of available genomic information for assay development. An in sil ...
... Herein we describe a highly structured, filamentous growth phenotype displayed by an isolate of the food spoilage microorganism Brochothrix thermosphacta. The growth morphology of this B. thermosphacta strain (strain BII) was dependent on environmental factors such as the growth media, incubation temperatures, and the inoculum concentration. Inoculation of cultures in highly dilute suspensions res ...
additives; carbon monoxide; chemical bonding; cleavage (chemistry); ethanol; ethylene glycol; glycerol; hot water treatment; hydrochloric acid; nanocomposites; nanocrystals; nanoparticles; nanorods; nanosheets; nitric acid; oxidation; palladium
Abstract:
... This work suggests a modest hydrothermal method applied for the synthesis of oxygen-deficient WOₓ (x = 2.75, 2.83, and 2.94) nanomaterials with various morphologies, such as bundled nanorods (NR), nanobelts (NB), and nanosheets (NS), by changing the inorganic additives, such as HCl, NaHSO₄, and HNO₃. In addition, WOₓ-supported high- and low-index faceted Pd nanoparticles (Pd–WO₂.₇₅ NB, Pd–WO₂.₈₃ N ...
ChinYiChen, et al. ; Yang Yang; Da Peng Liu; Ankit Raj; Hiro-o. Hamaguchi; Hui Bin Qiu; Yan Jun Lin; Chien Lung Wang; Xiao Song Wang; Show all 9 Authors
carbonyl compounds; hydrogen bonding; hydrophobicity; journals; metals; water
Abstract:
... Self-assembled vesicles with structured (tetrahedral order with strong hydrogen bonds) interstitial water are reported. The vesicles, known as MCsome, are assembled from metal carbonyl compounds, FpR (Fp = (Cp)Fe(PPh₃)(CO)(CO−), Cp = cyclopentadiene, R = C₃Bithiophene, C₆Pyrene or C₆) with the Fp heads exposed to water. The R groups are surrounded by the interstitial water with the hydrogen bondin ...
... DNA sequencing and other DNA-based methods are now broadly used for detection and identification of bacterial foodborne pathogens. For the identification of foodborne bacterial pathogens, taxonomic assignments must be made to the species or even subspecies level. Long-read DNA sequencing provides finer taxonomic resolution than short-read sequencing. Here, we demonstrate the potential of long-read ...
... Although chemical modifications on conjugated molecules are widely applied for the purpose of improving processability and device performances, the effect of the modification was far less investigated. Here, five S,N-hexacenes are studied to reveal the influences of (1) the lateral alkyl chain, (2) the terminal group (thiophene vs benzene), and (3) the end-capping phenyl group of the hexacenes on ...
... Among the polycyclic aromatic hydrocarbons, although perylene is commercially available and possesses higher solubility and stability than the others, its thin-film structures and organic field-effect transistor (OFET) performances have been rarely explored. To understand its potential as an active material in OFETs, the polymorphic behaviors, packing structures, and OFET characteristics of peryle ...
... In this research, manganese and nickel transition metal layered double hydroxide nanoparticles (MnNi(OH)₂) with a size of ∼100 nm have been successfully prepared using a sonochemical irradiation method for supercapacitor applications. X-ray diffraction (XRD) and Fourier Transform Infrared (FT-IR) analyses clearly indicate the formation of MnNi(OH)₂ nanoparticles. Scanning and Transmission electron ...
... Here, two diketopyrrolopyrrole (DPP)-based oligomers, DPP-4T and DPP-6T, are studied to reveal the influences of conjugation length on thin-film morphology and organic field-effect transistor (OFET) performances. PDMS-assisted crystallization in a solvent-annealing chamber is applied to prepare crystal arrays of DPP-4T and DPP-6T to optimize the quality of charge channels for OFET characterization ...
... Prophage insertions in Escherichia coli O157:H7 mlrA contribute to the low expression of curli fimbriae and biofilm observed in many clinical isolates. Varying levels of CsgD-dependent curli/biofilm expression are restored to strains bearing prophage insertions in mlrA by mutation of regulatory genes affecting csgD . Our previous study identified strong biofilm- and curli-producing variants in O15 ...
... Campylobacter jejuni , commonly found in poultry and meat products, causes gastroenteritis in humans. Here, we report the complete genome sequence of a C. jejuni strain, YH001, isolated from retail beef liver. The genome is 1,712,361 bp and has a 30.5% G+C content and two plasmids of 46.5 kb and 4.4 kb. ...
... A novel cloning vector to aid in the construction of single copy β-galactosidase reporter systems for gene expression studies in lactose metabolizing Escherichia coli strains, including STEC, is described. The plasmid allows construction of translational fusions of cloned gene promoters to a short segment of E. colilacZ. A selectable spectinomycin resistance marker flanked by a short lacI segment ...
... Biofilm formation in most Escherichia coli strains is dependent on curli fimbriae and cellulose, and the production of both varies widely among pathogenic strains. Curli and cellulose production by colonies growing on agar are often identified by their affinity for Congo red dye (CR). However, media composition and incubation temperature can affect dye affinity and impose limitations on red phenot ...
... In the present study, nanocrystalline photocatalyst Bi2O3 powders with various ZnO additions were synthesized by spray pyrolysis at 600 (SP600) and 700°C (SP700). The Bi2O3 powder converted from a monoclinic α phase to a tetragonal β phase at pyrolysis temperatures ≥600°C. The crystallinity of the SP powder increased with the increase in pyrolysis temperature. The morphology of the SP powders was ...
... A group of low molecular-weight ColE1-like plasmids carrying the aph sequence type aph(ii) from three different Salmonella serovars were sequenced. These plasmids carry two or more copies of IS903 elements, with up to 21bp sequence differences to one another, two of which flank the aph gene. This group of plasmids did not appear to carry any known mobilization genes and instead carry three open re ...
Chin-YiChen, et al. ; Christopher S. Hofmann; Bryan J. Cottrell; Terence P. Strobaugh Jr; George C. Paoli; Ly-Huong Nguyen; Xianghe Yan; Gaylen A. Uhlich; Mark Alexander Webber; Show all 9 Authors
... The biofilm life style helps bacteria resist oxidative stress, desiccation, antibiotic treatment, and starvation. Biofilm formation involves a complex regulatory gene network controlled by various environmental signals. It was previously shown that prophage insertions in mlrA and heterogeneous mutations in rpoS constituted major obstacles limiting biofilm formation and the expression of extracellu ...