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1. Summation of disinfection by-product CHO cell relative toxicity indices: sampling bias, uncertainty, and a path forward

Author:
Elizabeth McKenna; Kyle A. Thompson; Lizbeth Taylor-Edmonds; Daniel L. McCurry; David Hanigan
Source:
Environmental science 2020 v.22 no.3 pp. 708-718
ISSN:
2050-7895
Subject:
Chinese hamsters,   etc   ; activated carbon; animal ovaries; byproducts; cytotoxicity; disinfection; mutagens; uncertainty;     Show all 8 Subjects
Abstract:
... The cyto- and genotoxic potencies of disinfection by-products (DBPs) have been evaluated in published literature by measuring the response of exposed Chinese hamster ovary cells. In recent publications, DBP concentrations divided by their individual toxicity indices are summed to predict the relative toxicity of a water sample. We hypothesized that the omission or inclusion of certain DBPs over ot ...
DOI:
10.1039/c9em00468h

2. Kinetic modeling of Chinese hamster ovary cell culture: factors and principles

Author:
Peifeng Tang; Jianlin Xu; Alastair Louey; Zhijun Tan; Andrew Yongky; Shaoyan Liang; Zheng Jian Li; Yongyan Weng; Shijie Liu
Source:
Critical reviews in biotechnology 2020 v.40 no.2 pp. 265-281
ISSN:
1549-7801
Subject:
Chinese hamsters,   etc   ; animal ovaries; biopharmaceutical industry; bioreactors; cell culture; kinetics; manufacturing; protein synthesis; therapeutics;     Show all 9 Subjects
Abstract:
... As a host for therapeutic protein expression, Chinese hamster ovary (CHO) cells are widely utilized in the mainstream biopharmaceutical industry. Cell culture process development plays an important role in transitioning laboratory research to manufacturing. Among different mathematic tools, kinetic modeling is commonly achieved through analyzing cell culture data to design process parameters, opti ...
DOI:
10.1080/07388551.2019.1711015

3. Modelling of hollow fiber membrane bioreactor for mammalian cell cultivation using computational hydrodynamics

Author:
Natalia V. Menshutina; Elena V. Guseva; Ruslan R. Safarov; Joseph Boudrant
Source:
Bioprocess and biosystems engineering 2020 v.43 no.3 pp. 549-567
ISSN:
1615-7591
Subject:
Chinese hamsters,   etc   ; animal ovaries; cell growth; computer software; filtration; hydrodynamics; hydrologic models; mathematical models; membrane bioreactors;     Show all 9 Subjects
Abstract:
... The hollow fiber membrane bioreactor (HFMB) has been investigated for the cultivation of mammalian Chinese hamster ovary cell expansion. The experiments were carried out in Petri’s dishes and in the hollow fiber membrane bioreactor having 20 fibers (S2025 from FiberCell Systems). The approach to HFMB modelling which combines the model of cell growth kinetics and hydrodynamics has been proposed. Th ...
DOI:
10.1007/s00449-019-02249-9

4. Towards a new avenue for producing therapeutic proteins: Microalgae as a tempting green biofactory

Author:
Jaber Dehghani; Khosro Adibkia; Ali Movafeghi; Hadi Maleki-Kakelar; Nazli Saeedi; Yadollah Omidi
Source:
Biotechnology advances 2020 v.40 pp. 107499
ISSN:
0734-9750
Subject:
Chinese hamsters,   etc   ; animal ovaries; biopharmaceuticals; bioreactors; disulfide bonds; glycosylation; humans; microalgae; pathogens; purification methods; recombinant proteins;     Show all 11 Subjects
Abstract:
... Most of the recent approved therapeutic proteins are multi-subunit biologics, which need glycosylation and disulfide bridges for their correct conformation and biological functions. Currently, there exist many protein-based drugs that are mostly produced in the Chinese hamster ovary (CHO) cells. However, this expression system appears to associate with some limitations both in upstream and downstr ...
DOI:
10.1016/j.biotechadv.2019.107499

5. Developing an equivalent toxicity area approach to comparing toxicity of urban road deposited sediments

Author:
Yuting Zhan; Bo Yang; Nian Hong; Yingjie Guan; An Liu; Ye Du; Qianyuan Wu; Yuntao Guan
Source:
Environmental pollution 2020 v.257 pp. 113588
ISSN:
0269-7491
Subject:
Chinese hamsters,   etc   ; animal ovaries; case studies; pollutants; pollution; rain; regression analysis; sediment deposition; stormwater; surface area; toxicity; water reuse; water shortages; China;     Show all 14 Subjects
Abstract:
... Urban road stormwater has been seen as an alternative water resource for mitigating water scarcity. Road deposited sediments (RDS) is the primary source of road stormwater pollution since many toxic pollutants are attached to RDS and eventually enter stormwater runoff when rainfall events occur. This could threaten road stormwater reuse safety. This study developed an innovative equivalent toxicit ...
DOI:
10.1016/j.envpol.2019.113588

6. Ascorbic Acid 2-Glucoside Pretreatment Protects Cells from Ionizing Radiation, UVC, and Short Wavelength of UVB

Author:
Junko Maeda; Allison J. Allum; Jacob T. Mussallem; Coral E. Froning; Alexis H. Haskins; Mark A. Buckner; Chris D. Miller; Takamitsu A. Kato
Source:
Genes 2020 v.11 no.3 pp. -
ISSN:
2073-4425
Subject:
Chinese hamsters,   etc   ; DNA damage; animal ovaries; ascorbic acid; bioavailability; cell death; cytotoxicity; dimethyl sulfoxide; filtration; protective effect; spectrometers; spectroradiometers; ultraviolet radiation; wavelengths;     Show all 14 Subjects
Abstract:
... Ascorbic acid 2-glucoside (AA2G), glucosylated ascorbic acid (AA), has superior properties for bioavailability and stability compared to AA. Although AA2G has shown radioprotective properties similar to AA, effects for UV light, especially UVC and UVB, are not studied. AA2G was tested for cytotoxicity and protective effects against ionizing radiation, UVC, and broadband and narrowband UVB in Chine ...
DOI:
10.3390/genes11030238

7. Human CYP1B1-dependent genotoxicity of dioxin-like polychlorinated biphenyls in mammalian cells

Author:
Yuting Chen; Yifan Wu; Weiwei Xiao; Hansi Jia; Hansruedi Glatt; Ming Shi; Yungang Liu
Source:
Toxicology 2020 v.429 pp. 152329
ISSN:
0300-483X
Subject:
Chinese hamsters,   etc   ; DNA damage; carcinogens; catfish; centromeres; genetic engineering; genotoxicity; hepatoma; human diseases; humans; metabolism; mutagens; persistent organic pollutants; polychlorinated biphenyls; staining;     Show all 15 Subjects
Abstract:
... Polychlorinated biphenyls (PCBs) are persistent organic pollutants and human carcinogens. It was reported that rat CYP1A1 and catfish CYP1A can hydroxylate 3,3',4,4',5-pentachlorobiphenyl (PCB 126) and 3,3',4,4'-tetrachlorobiphenyl (PCB 77), while potential roles of other CYP1 enzymes in the metabolism of dioxin-like (DL) PCBs remain unconfirmed. In this study, three representative DL-PCBs, i.e., ...
DOI:
10.1016/j.tox.2019.152329

8. Transient manipulation of the expression level of selected growth rate correlating microRNAs does not increase growth rate in CHO-K1 cells

Author:
Gerald Klanert; Nina Bydlinski; Patrice Agu; Andreas B. Diendorfer; Matthias Hackl; Michael Hanscho; Michael Melcher; Martina Baumann; Johannes Grillari; Nicole Borth
Source:
Journal of biotechnology 2019 v.295 pp. 63-70
ISSN:
0168-1656
Subject:
Chinese hamsters,   etc   ; animal ovaries; engineering; gene expression regulation; microRNA; phenotype; plasmids; transfection;     Show all 8 Subjects
Abstract:
... Engineering of Chinese Hamster Ovary cells by manipulating microRNA (miRNA) expression levels has been shown to induce advantageous, desired phenotypes. Most of these studies so far were concerned with increasing productivity or reducing growth rate (with the implied intention of thus freeing cellular resources to also increase productivity). Here we evaluated the ability of growth correlating miR ...
DOI:
10.1016/j.jbiotec.2019.02.011

9. Thermal expansion of substrate may affect adhesion of Chinese hamster fibroblasts to surfaces during freezing

Author:
Taylor Rutt; Nasim Eskandari; Maria Zhurova; Janet A.W. Elliott; Locksley E. McGann; Jason P. Acker; John A. Nychka
Source:
Cryobiology 2019 v.86 pp. 134-139
ISSN:
0011-2240
Subject:
Chinese hamsters,   etc   ; cryopreservation; fibroblasts; freezing; glass; ice; stress relaxation; temperature; thermal expansion;     Show all 9 Subjects
Abstract:
... Despite success in cryopreservation of cells in suspension, cryopreservation of cells in monolayers is still challenging. One of the major problems is detachment of the cells from the substrate which occurs during cryopreservation. We hypothesized that this detachment may be due to a mismatch in the coefficient of linear thermal expansion αL between glass and the frozen cell layer which manifests ...
DOI:
10.1016/j.cryobiol.2018.10.006

10. Surrogates for the removal by ozonation of the cytotoxicity and DNA double-strand break effects of wastewater on mammalian cells

Author:
Ye Du; Yang Yang; Wen-Long Wang; Yu-Ting Zhou; Qian-Yuan Wu
Source:
Environment international 2020 v.135 pp. 105369
ISSN:
0160-4120
Subject:
Chinese hamsters,   etc   ; DNA; animal ovaries; cell viability; cytotoxicity; effluents; fluorescence; fluorescent dyes; genotoxicity; molecular weight; organic matter; ozonation; ozone; wastewater; wastewater treatment; water quality;     Show all 16 Subjects
Abstract:
... Effluents from wastewater treatment plants (WWTPs) may contain various pollutants with potential toxic effects. Ozonation is widely applied to purify wastewater, which may influence the toxicity and water quality indices simultaneously. The main goal of this study was to reveal influence of ozonation on toxicity of WWTP effluents and to find the surrogates for toxicity changes. Cytotoxicity and DN ...
DOI:
10.1016/j.envint.2019.105369

11. The use of site-specific recombination and cassette exchange technologies for monoclonal antibody production in Chinese Hamster ovary cells: retrospective analysis and future directions

Author:
Kajan Srirangan; Martin Loignon; Yves Durocher
Source:
Critical reviews in biotechnology 2020 v.40 no.6 pp. 833-851
ISSN:
1549-7801
Subject:
Chinese hamsters,   etc   ; animal ovaries; antibody formation; biopharmaceuticals; cell lines; clinical examination; clones; industry; monoclonal antibodies; plasmids; recombinases; retrospective studies; site-specific recombination; therapeutics; transgenes;     Show all 15 Subjects
Abstract:
... Chinese hamster ovary (CHO) cell-based platforms are the most widely used for the biomanufacturing of complex therapeutic proteins, such as monoclonal antibodies (mAbs). The development of high-producing clones that are stable and amenable to large-scale cultures is essential to advance a molecule toward clinical evaluation. Nevertheless, the generation of such clones generally relies on random in ...
DOI:
10.1080/07388551.2020.1768043

12. Evidence for diversity in the activation of the melanocortin 2 receptor: A study on gar, elephant shark and stingray MC2Rs

Author:
Brianne E. Hoglin; Amanda Ferguson; Sheila Pahlavan; Robert M. Dores
Source:
Peptides 2020 v.124 pp. 170209
ISSN:
0196-9781
Subject:
Chinese hamsters,   etc   ; Chondrichthyes; Lepisosteus oculatus; alanine; animal ovaries; corticotropin; ligands; parallel evolution; sharks;     Show all 9 Subjects
Abstract:
... The melanocortin-2 receptor (MC2R) is a critical component of the HPI and HPA axes of cartilaginous fishes, teleosts and tetrapods. Studies on teleost and tetrapod orthologs suggest two contact sites between ACTH and the receptor involving the following motifs on ACTH: H⁶F⁷R⁸W⁹ and K¹⁵K¹⁶R¹⁷R¹⁸P¹⁹. Using spotted gar (g) MC2R as a representative bony fish MC2R ortholog, we found that activation of ...
DOI:
10.1016/j.peptides.2019.170209

13. Enzyme capacity-based genome scale modelling of CHO cells

Author:
Hock Chuan Yeo; Jongkwang Hong; Meiyappan Lakshmanan; Dong-Yup Lee
Source:
Metabolic engineering 2020 v.60 pp. 138-147
ISSN:
1096-7176
Subject:
Chinese hamsters,   etc   ; animal ovaries; bioprocessing; case studies; cell culture; cell engineering; cell lines; enzyme kinetics; enzymes; genome; glucose; lactic acid; mitochondria; models; oxidative phosphorylation; prediction;     Show all 16 Subjects
Abstract:
... Chinese hamster ovary (CHO) cells are most prevalently used for producing recombinant therapeutics in biomanufacturing. Recently, more rational and systems approaches have been increasingly exploited to identify key metabolic bottlenecks and engineering targets for cell line engineering and process development based on the CHO genome-scale metabolic model which mechanistically characterizes cell c ...
DOI:
10.1016/j.ymben.2020.04.005

14. Genotoxicity evaluation of tobacco and nicotine delivery products: Part Two. In vitro micronucleus assay

Author:
David Thorne; Robert Leverette; Damien Breheny; Mel Lloyd; Stephen McEnaney; James Whitwell; Julie Clements; Betsy Bombick; Marianna Gaça
Source:
Food and chemical toxicology 2019 v.132 pp. 110546
ISSN:
0278-6915
Subject:
Chinese hamsters,   etc   ; cigarettes; electronic equipment; genotoxicity; human cell lines; humans; micronucleus tests; nicotine; particulates; Kentucky;     Show all 10 Subjects
Abstract:
... In this study, a variety of test matrices from tobacco and nicotine delivery products were assessed against a 3R4F Kentucky reference cigarette using the in vitro micronucleus assay. Testing was conducted using two Chinese hamster cell lines (CHO and V79), and a human lymphoblastoid cell line (TK6), in accordance with established guidelines.Total particulate matter (TPM) from a 3R4F Reference ciga ...
DOI:
10.1016/j.fct.2019.05.054

15. Model-assisted Design of Experiments as a concept for knowledge-based bioprocess development

Author:
Johannes Möller; Kim B. Kuchemüller; Tobias Steinmetz; Kirsten S. Koopmann; Ralf Pörtner
Source:
Bioprocess and biosystems engineering 2019 v.42 no.5 pp. 867-882
ISSN:
1615-7591
Subject:
Chinese hamsters,   etc   ; animal ovaries; bioprocessing; experimental design; models;     Show all 5 Subjects
Abstract:
... Design of Experiments methods offer systematic tools for bioprocess development in Quality by Design, but their major drawback is the user-defined choice of factor boundary values. This can lead to several iterative rounds of time-consuming and costly experiments. In this study, a model-assisted Design of Experiments concept is introduced for the knowledge-based reduction of boundary values. First ...
DOI:
10.1007/s00449-019-02089-7

16. DNA retention on depth filters

Author:
Ohnmar Khanal; Xuankuo Xu; Nripen Singh; Steven J. Traylor; Chao Huang; Sanchayita Ghose; Zheng Jian Li; Abraham M. Lenhoff
Source:
Journal of membrane science 2019 v.570-571 pp. 464-471
ISSN:
0376-7388
Subject:
Chinese hamsters,   etc   ; DNA; animal ovaries; artificial membranes; bioprocessing; filters; filtration; fluorescence microscopy; ionic strength; protein aggregates;     Show all 10 Subjects
Abstract:
... Depth filtration is a commonly-used bioprocessing unit operation for harvest clarification that reduces the levels of process- and product-related impurities such as cell debris, host-cell proteins, nucleic acids and protein aggregates. Since depth filters comprise multiple components, different functionalities may contribute to such retention, making the mechanisms by which different impurities a ...
DOI:
10.1016/j.memsci.2018.10.058
PubMed:
31223185
PubMed Central:
PMC6586439

17. Insights into the loss of protein sialylation in an fc-fusion protein-producing CHO cell bioprocess

Author:
Xinning Chen; Xuping Liu; Zheng Xiao; Jintao Liu; Liang Zhao; Wen-Song Tan; Li Fan
Source:
Applied microbiology and biotechnology 2019 v.103 no.12 pp. 4753-4765
ISSN:
0175-7598
Subject:
Chinese hamsters,   etc   ; animal ovaries; bioprocessing; biosynthesis; glycoproteins; glycosylation; half life; hydrolysis; manufacturing; sialic acid; sialidase; therapeutics;     Show all 12 Subjects
Abstract:
... Sialylation affects circulating half-life, charge distribution, and other biochemical properties of therapeutic glycoproteins. Loss of protein sialylation during glycoprotein-producing bioprocesses could lead to a low final protein sialylation level and bring negative effects on subsequent clinical efficacy. In this work, an Fc-fusion protein-producing Chinese hamster ovary cell fed-batch culture ...
DOI:
10.1007/s00253-019-09850-8

18. Predicting the cytotoxicity of disinfection by-products to Chinese hamster ovary by using linear quantitative structure–activity relationship models

Author:
Li-Tang Qin; Xin Zhang; Yu-Han Chen; Ling-Yun Mo; Hong-Hu Zeng; Yan-Peng Liang; Hua Lin; Dun-Qiu Wang
Source:
Environmental science and pollution research 2019 v.26 no.16 pp. 16606-16615
ISSN:
0944-1344
Subject:
Chinese hamsters,   etc   ; animal ovaries; byproducts; cytotoxicity; disinfection; linear models; prediction; quantitative structure-activity relationships; regression analysis;     Show all 9 Subjects
Abstract:
... A suitable model to predict the toxicity of current and continuously emerging disinfection by-products (DBPs) is needed. This study aims to establish a reliable model for predicting the cytotoxicity of DBPs to Chinese hamster ovary (CHO) cells. We collected the CHO cytotoxicity data of 74 DBPs as the endpoint to build linear quantitative structure–activity relationship (QSAR) models. The linear mo ...
DOI:
10.1007/s11356-019-04947-z

19. The fickle CHO: a review of the causes, implications, and potential alleviation of the CHO cell line instability problem

Author:
Hussain Dahodwala; Kelvin H Lee
Source:
Current opinion in biotechnology 2019 v.60 pp. 128-137
ISSN:
0958-1669
Subject:
Chinese hamsters,   etc   ; animal ovaries; cell lines; genetic engineering; genome; genomics; plasticity; prediction; product quality; recombinant proteins;     Show all 10 Subjects
Abstract:
... Chinese hamster ovary (CHO) cell-based bioproduction of recombinant proteins can now routinely achieve >5 g/L titers in fed-batches. This progress is partly due to the rapid adaptability of CHO cells to various genetic manipulations and changing process conditions. An inherently plastic genome allows for this adaptability; however, it also gives CHO cells the propensity for genomic rearrangements. ...
DOI:
10.1016/j.copbio.2019.01.011

20. Virus removal filtration of chemically defined Chinese Hamster Ovary cells medium with nanocellulose-based size exclusion filter

Author:
Levon Manukyan; Justine Padova; Albert Mihranyan
Source:
Biologicals 2019 v.59 pp. 62-67
ISSN:
1045-1056
Subject:
Chinese hamsters,   etc   ; Parvoviridae; animal ovaries; bacteriophages; bioprocessing; bioreactors; cell culture; cell viability; cost effectiveness; filters; filtration;     Show all 11 Subjects
Abstract:
... Sterility of bioreactors in biotherapeutic processing remains a significant challenge. Virus removal size-exclusion filtration is a robust and highly efficient approach to remove viruses. This article investigates the virus removal capacity of nanocellulose-based filter for upstream bioprocessing of chemically defined Chinese hamster ovary (CHO) cells medium containing Pluronic F-68 (PowerCHO™, Lo ...
DOI:
10.1016/j.biologicals.2019.03.001