You searched for:
Author
"Claudio L. Afonso"
Remove constraint Author: "Claudio L. Afonso"
PubAg
Main content area
Limit your search
Search
74 Search Results
« Previous |
1 - 20 of 74
|
Next »
Search Results
- Author:
- Claudio L. Afonso
- Source:
- Viruses 2021 v.13 no.1 pp. -
- ISSN:
- 1999-4915
- Subject:
- Newcastle disease; adults; mutation; pathogenesis; pathotypes; phylogeny; virulence
- Abstract:
- ... The hypothesis that host adaptation in virulent Newcastle disease viruses (NDV) has been accompanied by virulence modulation is reviewed here. Historical records, experimental data, and phylogenetic analyses from available GenBank sequences suggest that currently circulating NDVs emerged in the 1920–1940′s from low virulence viruses by mutation at the fusion protein cleavage site. These viruses la ...
- DOI:
- 10.3390/v13010110
- https://doi.org/10.3390/v13010110
- Author:
- Claudio L. Afonso, et al. ; Iryna V. Goraichuk; James F. Davis; Arun B. Kulkarni; David L. Suarez; Show all 5 Authors
- Source:
- Microbiology resource announcements 2020 v.9 no.9 pp. -
- ISSN:
- 2576-098X
- Subject:
- Infectious bronchitis virus; broiler chickens; fecal examination; feces; infectious bronchitis; nucleotide sequences; viral genome; virulent strains; Georgia
- Abstract:
- ... Avian coronavirus, also known as infectious bronchitis virus, is a highly contagious respiratory pathogen of chickens that is responsible for major economic losses to the poultry industry around the globe. Here, we report the complete genome sequence of strain GA08 of the GI-27 lineage, isolated from a fecal sample from a broiler chicken collected in Georgia in 2015. ...
- Handle:
- 10113/7058196
- DOI:
- 10.1128/MRA .00068-20
- https://doi.org/10.1128/MRA .00068-20
- Author:
- Claudio L. Afonso, et al. ; Qingzhong Yu; Yufeng Li; Kiril Dimitrov; Stephen Spatz; Laszlo Zsak; Show all 6 Authors
- Source:
- Vaccine 2020 v.38 no.4 pp. 925-932
- ISSN:
- 0264-410X
- Subject:
- Avian orthoavulavirus 1; Gallid alphaherpesvirus 1; chick embryos; chicken eggs; chickens; fluorescent antibody technique; genes; genetic stability; glycoproteins; growth models; high-throughput nucleotide sequencing; recombinant vaccines; reverse transcriptase polymerase chain reaction; single nucleotide polymorphism; virulence; viruses
- Abstract:
- ... Previously, we have demonstrated that the recombinant Newcastle disease virus (NDV) expressing the infectious laryngotracheitis virus (ILTV) glycoprotein D (gD) conferred protection against both virulent NDV and ILTV challenges in chickens. In this study, we evaluated the genetic stability of the recombinant vaccine after eight serial passages in embryonated chicken eggs (ECE). The vaccine master ...
- DOI:
- 10.1016/j.vaccine.2019.10.074
- CHORUS:
- 10.1016/j.vaccine.2019.10.074
- https://doi.org/10.1016/j.vaccine.2019.10.074
- Author:
- Claudio L. Afonso, et al. ; Stivalis Cardenas-Garcia; Show all 2 Author
- Source:
- Methods in Molecular Biology 2017 v.1602 no. pp. 141-158
- ISSN:
- 1064-3745
- Subject:
- gene therapy; molecular biology; complementary DNA; bacteriophages; plasmids; Avian orthoavulavirus 1; genes; Vaccinia virus; vaccine development; site-directed mutagenesis; gene deletion; DNA-directed RNA polymerase
- Abstract:
- ... Reverse genetics allows for the generation of recombinant viruses or vectors used in functional studies, vaccine development, and gene therapy. This technique enables genetic manipulation and cloning of viral genomes, gene mutation through site-directed mutagenesis, along with gene insertion or deletion, among other studies. An in vitro infection-based system including the highly attenuated vaccin ...
- Handle:
- 10113/5763048
- DOI:
- 10.1007/978-1-4939-6964-7_10
- PubMed:
- 28508219
- PubMed Central:
- PMC7120584
- https://doi.org/10.1007/978-1-4939-6964-7_10
- Author:
- Claudio L. Afonso, et al. ; Henry M. Kariithi; Helena L. Ferreira; Catharine N. Welch; Leonard O. Ateya; Auleria A. Apopo; Richard Zoller; Jeremy D. Volkening; Dawn Williams-Coplin; Darren J. Parris; Tim L. Olivier; Dana Goldenberg; Yatinder S. Binepal; Sonia M. Hernandez; David L. Suarez; Show all 15 Authors
- Source:
- Viruses 2021 v.13 no.1 pp. -
- ISSN:
- 1999-4915
- Subject:
- Avian orthoavulavirus 1; Newcastle disease; amino acids; chickens; farms; genome; monitoring; phylogeography; subgenotype; surveys; trade; virulence; viruses; Kenya
- Abstract:
- ... Kenyan poultry consists of ~80% free-range indigenous chickens kept in small flocks (~30 birds) on backyard poultry farms (BPFs) and they are traded via live bird markets (LBMs). Newcastle disease virus (NDV) was detected in samples collected from chickens, wild farm birds, and other domestic poultry species during a 2017–2018 survey conducted at 66 BPFs and 21 LBMs in nine Kenyan counties. NDV nu ...
- DOI:
- 10.3390/v13010103
- https://doi.org/10.3390/v13010103
- Author:
- Claudio L. Afonso, et al. ; Valerie Marcano; Stivalis Cardenas-Garcia; Robert M. Gogal; Show all 4 Authors
- Source:
- Journal of virological methods 2018 v.251 pp. 1-6
- ISSN:
- 0166-0934
- Subject:
- chickens; phenotype; antigens; antibodies; allantoic fluid; heat; flow cytometry; ligands; macrophages; Avian orthoavulavirus 1; splenocytes; phosphates; formalin; ultraviolet radiation
- Abstract:
- ... Inactivation of Newcastle disease virus (NDV) has been routinely achieved with heat, β-propiolactone, binary ethylenimine, ultraviolet light and formalin. However, these strategies have not been tested for cell surface ligand or receptor phenotype in viral-infected chicken immune cells. To study the capacity of fixation buffers to preserve surface markers while inactivating NDV, a primary splenocy ...
- Handle:
- 10113/5838019
- DOI:
- 10.1016/j.jviromet.2017.09.025
- https://doi.org/10.1016/j.jviromet.2017.09.025
- Author:
- Claudio L. Afonso, et al. ; Helena L. Ferreira; Tonya L. Taylor; Kiril M. Dimitrov; Mahmoud Sabra; David L. Suarez; Show all 6 Authors
- Source:
- Veterinary microbiology 2019 v.235 pp. 25-34
- ISSN:
- 0378-1135
- Subject:
- chickens; Newcastle disease; hosts; Avian orthoavulavirus 1; viruses; genotype; seroconversion; viral load; viral shedding; wild birds; virulence; mortality; signs and symptoms (animals and humans)
- Abstract:
- ... Five, class II, virulent Newcastle disease virus (vNDV) isolates of different genotypes from different host species were evaluated for their ability to infect, cause disease, and transmit to naïve chickens. Groups of five birds received a low, medium, or high dose, by the oculonasal route, of one of the following vNDV: three chicken-origin, one cormorant-origin, and one pigeon-origin. Three naïve ...
- Handle:
- 10113/6461958
- DOI:
- 10.1016/j.vetmic.2019.06.004
- CHORUS:
- 10.1016/j.vetmic.2019.06.004
- Chorus Open Access:
- 10.1016/j.vetmic.2019.06.004
- https://doi.org/10.1016/j.vetmic.2019.06.004
- Author:
- Claudio L. Afonso, et al. ; Auleria A. Apopo; Henry M. Kariithi; Leonard O. Ateya; Yatinder S. Binepal; Jane H. Sirya; Thomas D. Dulu; Catharine N. Welch; Sonia M. Hernandez; Show all 9 Authors
- Source:
- Tropical animal health and production 2020 v.52 no.2 pp. 699-710
- ISSN:
- 0049-4747
- Subject:
- Avian orthoavulavirus 1; Newcastle disease; agroecological zones; ambient temperature; animal viruses; chickens; climate; diagnostic techniques; farmers; indigenous species; introduced species; monitoring; mortality; poultry production; production technology; relative humidity; retrospective studies; Kenya
- Abstract:
- ... Newcastle disease (ND) is a major constraint to Kenya’s poultry production, which is comprised of approximately 80% indigenous chickens (ICs; caged and free-range system) and 20% exotic chickens (intensive system). This study analyzed cases reported as suspected ND in Kenya between 2005 and 2015. Of the suspected 332 ND reported cases from the three production systems in 27 locations within six Ke ...
- DOI:
- 10.1007/s11250-019-02059-x
- PubMed:
- 31501991
- PubMed Central:
- PMC7039849
- https://doi.org/10.1007/s11250-019-02059-x
- Author:
- Claudio L. Afonso, et al. ; Mustafa Ababneh; Helena L. Ferreira; Mohammad Khalifeh; David L. Suarez; Show all 5 Authors
- Source:
- Microbiology resource announcements 2018 v.7 no.23 pp. e01136
- ISSN:
- 2576-098X
- Subject:
- Avian orthoavulavirus 1; RNA; chickens; molecular systematics; nucleotide sequences; phylogeny; spleen; subgenotype; viral genome; Jordan
- Abstract:
- ... Newcastle disease virus (NDV) was detected by reverse transcriptase PCR (RT-PCR) from total RNA isolated from a chicken spleen of a backyard flock in Jordan. The complete coding genome sequence of NDV/chicken/Jordan/J11-spleen/2018 was obtained with MiSeq (Illumina) sequencing. Phylogenetic analysis of the concatenated coding sequences classified the virus as class II subgenotype VIIi. ...
- Handle:
- 10113/7382910
- DOI:
- 10.1128/MRA.01136-18
- https://doi.org/10.1128/MRA.01136-18
- Author:
- Claudio L. Afonso, et al. ; Stephen J. Spatz; Maricarmen Garcia; Sylva Riblet; Teresa A. Ross; Jeremy D. Volkening; Tonya L. Taylor; Taejoong Kim; Show all 8 Authors
- Source:
- Avian pathology 2019 v.48 no.3 pp. 255-269
- ISSN:
- 1465-3338
- Subject:
- restriction fragment length polymorphism; allelic variation; Gallid alphaherpesvirus 1; nanopores; alleles; monitoring; open reading frames; flocks; industry; polymerase chain reaction; phylogeny; genotype; genotyping; virulence; birds; single nucleotide polymorphism; United States
- Abstract:
- ... Over the last decade the US broiler industry has fought long-lasting outbreaks of infectious laryngotracheitis (ILTV). Previously, nine genotypes (I-IX) of ILTVs have been recognized using the polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) method with three viral alleles (gB, gM and UL47/gG). In this study, the genotyping system was simplified to six genotypes by am ...
- Handle:
- 10113/6372540
- DOI:
- 10.1080/03079457.2019.1579298
- https://doi.org/10.1080/03079457.2019.1579298
- Author:
- Claudio L. Afonso, et al. ; Letícia B. de Oliveira; James B. Stanton; Jian Zhang; Corrie Brown; Salman Latif Butt; Kiril Dimitrov; Jeremy D. Volkening; Leonardo J. C. Lara; Camila Stefanie F. de Oliveira; Roselene Ecco; Show all 11 Authors
- Source:
- Veterinary pathology 2021 v.58 no.1 pp. 123-135
- ISSN:
- 1544-2217
- Subject:
- Avian nephritis virus; RNA; Rotavirus; animal pathology; atrophy; epidemiology; epithelium; etiology; genes; genetic similarity; histology; histopathology; hybridization; ileum; jejunum; pathogenesis; serosa
- Abstract:
- ... Runting stunting syndrome (RSS) in commercial chickens has been reported worldwide, and although several studies have attempted to clarify the cause and describe the lesions, there are gaps in knowledge of the epidemiology, pathogenesis, and etiology. The study objective was to use commercial chicks naturally affected by RSS to describe the histologic changes of RSS in all segments of the small in ...
- DOI:
- 10.1177/0300985820969971
- CHORUS:
- 10.1177/0300985820969971
- https://doi.org/10.1177/0300985820969971
- Author:
- Claudio L. Afonso, et al. ; Kiril M. Dimitrov; Qingzhong Yu; Patti J. Miller; Show all 4 Authors
- Source:
- Veterinary microbiology 2017 v.206 pp. 126-136
- ISSN:
- 0378-1135
- Subject:
- chickens; neutralization; Newcastle disease; research and development; biosecurity; antigens; gastrointestinal system; inactivated vaccines; antibodies; flocks; animal health; Avian orthoavulavirus 1; villages; immunization; death; virulence; mortality; virulent strains
- Abstract:
- ... Newcastle disease (ND) has been defined by the World Organisation for Animal Health as infection of poultry with virulent strains of Newcastle disease virus (NDV). Lesions affecting the neurological, gastrointestinal, respiratory, and reproductive systems are most often observed. The control of ND must include strict biosecurity that prevents virulent NDV from contacting poultry, and also proper a ...
- Handle:
- 10113/5734383
- DOI:
- 10.1016/j.vetmic.2016.12.019
- PubMed:
- 28024856
- PubMed Central:
- PMC7131810
- https://doi.org/10.1016/j.vetmic.2016.12.019
- Author:
- Claudio L. Afonso, et al. ; Tonya L. Taylor; Kiril M. Dimitrov; Show all 3 Authors
- Source:
- Infection, genetics, and evolution 2017 v.50 pp. 28-37
- ISSN:
- 1567-1348
- Subject:
- Gallus gallus; transcription (genetics); codons; Avian orthoavulavirus 1; viruses; genotype; birds
- Abstract:
- ... In order to characterize the evolutionary adaptations of avian paramyxovirus 1 (APMV-1) genomes, we have compared codon usage and codon adaptation indexes among groups of Newcastle disease viruses that differ in biological, ecological, and genetic characteristics. We have used available GenBank complete genome sequences, and compared codon usage of class I (CI-29 sequences containing 132,675 codon ...
- DOI:
- 10.1016/j.meegid.2017.02.004
- CHORUS:
- 10.1016/j.meegid.2017.02.004
- Chorus Open Access:
- 10.1016/j.meegid.2017.02.004
- https://doi.org/10.1016/j.meegid.2017.02.004
- Author:
- Claudio L. Afonso, et al. ; Salman Latif Butt; Kiril M. Dimitrov; Jian Zhang; Abdul Wajid; Tasra Bibi; Asma Basharat; Corrie C. Brown; Shafqat F. Rehmani; James B. Stanton; Show all 10 Authors
- Source:
- Virus genes 2019 v.55 no.4 pp. 502-512
- ISSN:
- 0920-8569
- Subject:
- Avian orthoavulavirus 1; Newcastle disease; RNA; brain; chickens; data analysis; disease control; disease outbreaks; epidemiological studies; flocks; genes; high-throughput nucleotide sequencing; intestines; lungs; nucleotide sequences; phylogeny; poultry production; spleen; subgenotype; tissues; virulence; viruses; Pakistan
- Abstract:
- ... Highly virulent Newcastle disease virus (NDV) causes Newcastle disease (ND), which is a threat to poultry production worldwide. Effective disease management requires approaches to accurately determine sources of infection, which involves tracking of closely related viruses. Next-generation sequencing (NGS) has emerged as a research tool for thorough genetic characterization of infectious organisms ...
- DOI:
- 10.1007/s11262-019-01669-9
- https://doi.org/10.1007/s11262-019-01669-9
- Author:
- Claudio L. Afonso, et al. ; Iryna V. Goraichuk; Peter L. M. Msoffe; Gaspar H. Chiwanga; Kiril M. Dimitrov; David L. Suarez; Show all 6 Authors
- Source:
- Microbiology resource announcements 2019 v.8 no.27 pp. -
- ISSN:
- 2576-098X
- Subject:
- Avian orthoavulavirus 1; chickens; live bird markets; nucleotide sequences; subgenotype; viral genome; Tanzania
- Abstract:
- ... A Newcastle disease virus was isolated from a chicken from a live birdmarket in the Mbeya region of Tanzania. Complete genome characterization of the isolate identified it as a member of subgenotype Vd. This is the first complete genome sequence of this subgenotype. ...
- Handle:
- 10113/6837728
- DOI:
- 10.1128/MRA.00436-19
- PubMed:
- 31270191
- PubMed Central:
- PMC6606905
- https://doi.org/10.1128/MRA.00436-19
- Author:
- Claudio L Afonso
- Source:
- Journal of Clinical Microbiology 2013 v.51 no.2 pp. 508-517
- ISSN:
- 1098-660X
- Subject:
- chickens; sequence analysis; Newcastle disease; viral fusion proteins; lymphatic system; lesions (animal); monitoring; morbidity; genome; phylogeny; disease outbreaks; Avian orthoavulavirus 1; viruses; genotype; wild birds; phenylalanine; virulence; mortality; Dominican Republic
- Abstract:
- ... Newcastle disease (ND) is caused by Newcastle disease virus (NDV) and causes significant morbidity and mortality in most bird species. An ND outbreak was recently reported in the Dominican Republic in 2008 and was determined to be caused by NDV. The complete genome of one isolate and the fusion protein of three other related viruses were sequenced and phylogenetically analyzed to determine that th ...
- Handle:
- 10113/57514
- DOI:
- 10.1128/JCM.02393-12
- PubMed:
- 23196361
- PubMed Central:
- PMC3553931
- https://doi.org/10.1128/JCM.02393-12
- Author:
- Claudio L. Afonso, et al. ; Henry M. Kariithi; Catharine N. Welch; Helena L. Ferreira; Elizabeth A. Pusch; Leonard O. Ateya; Yatinder S. Binepal; Auleria A. Apopo; Thomas D. Dulu; David L. Suarez; Show all 10 Authors
- Source:
- Infection, genetics, and evolution 2020 v.78 pp. 104074
- ISSN:
- 1567-1348
- Subject:
- Influenza A virus; avian influenza; direct contact; evolution; infection; mammals; monitoring; mortality; nucleotide sequences; pathogenesis; poultry production; sequence analysis; seroconversion; viruses; zoonoses; Kenya
- Abstract:
- ... Poultry production plays an important role in the economy and livelihoods of rural households in Kenya. As part of a surveillance program, avian influenza virus (AIV)-specific real-time RT-PCR (RRT-PCR) was used to screen 282 oropharyngeal swabs collected from chickens at six live bird markets (LBMs) and 33 backyard poultry farms in Kenya and 8 positive samples were detected. Virus was isolated in ...
- DOI:
- 10.1016/j.meegid.2019.104074
- CHORUS:
- 10.1016/j.meegid.2019.104074
- https://doi.org/10.1016/j.meegid.2019.104074
- Author:
- Claudio L. Afonso, et al. ; Ariel Vagnozzi; Sylva Riblet; Guillermo Zavala; Roselene Ecco; Maricarmen García; Show all 6 Authors
- Source:
- Avian pathology 2016 v.45 no.1 pp. 106-113
- ISSN:
- 1465-3338
- Subject:
- chickens; Gallid alphaherpesvirus 1; interferon-gamma; interleukin-18; poultry industry; vaccines; interleukin-6; virulence; financial economics; transcription (genetics); gene expression; viruses; genes; immune response; vaccination; conjunctiva; interferon-beta; interleukin-1beta; virus replication; interleukin-8
- Abstract:
- ... Infectious laryngotracheitis is a highly contagious disease of chickens responsible for significant economic losses for the poultry industry worldwide. The disease is caused by Gallid herpesvirus-1 (GaHV-1) commonly known as the infectious laryngotracheitis virus. Although characterized by their potential to regain virulence, chicken embryo origin (CEO) vaccines are the most effective vaccines aga ...
- Handle:
- 10113/62704
- DOI:
- 10.1080/03079457.2015.1126804
- https://doi.org/10.1080/03079457.2015.1126804
19. Experimental Infection and Transmission of Newcastle Disease Vaccine Virus in Four Wild Passerines
- Author:
- Claudio L. Afonso, et al. ; Andrea J. Ayala; Sonia M. Hernandez; Timothy L. Olivier; Catherine N. Welch; Kiril M. Dimitrov; Iryna V. Goraichuk; Patti J. Miller; Show all 8 Authors
- Source:
- Avian diseases 2019 v.63 no.3 pp. 389-399
- ISSN:
- 0005-2086
- Subject:
- Avian orthoavulavirus 1; Cardinalis cardinalis; Carpodacus mexicanus; Molothrus ater; Newcastle disease; White Leghorn; adults; antibodies; antibody formation; blood; chickens; cloaca; drinking water; hemagglutination; live vaccines; monitoring; reverse transcriptase polymerase chain reaction; signs and symptoms (animals and humans); specific pathogen-free animals; viral shedding; viruses; wild birds
- Abstract:
- ... Our prior work has shown that live poultry vaccines have been intermittently isolated from wild birds sampled during field surveillance studies for Newcastle disease virus (NDV). Thus, we experimentally investigated the susceptibility of four native agriculturally associated wild bird species to the NDV LaSota vaccine and evaluated the shedding dynamics, potential transmission from chickens, and h ...
- Handle:
- 10113/6743214
- DOI:
- 10.1637/11980-092918-Reg.1
- https://doi.org/10.1637/11980-092918-Reg.1
- Author:
- Claudio L. Afonso, et al. ; Catharine N. Welch; Ismaila Shittu; Celia Abolnik; Ponman Solomon; Kiril M. Dimitrov; Tonya L. Taylor; Dawn Williams-Coplin; Iryna V. Goraichuk; Clement A. Meseko; John O. Ibu; Dorcas A. Gado; Tony M. Joannis; Show all 13 Authors
- Source:
- Archives of virology 2019 v.164 no.8 pp. 2031-2047
- ISSN:
- 0304-8608
- Subject:
- Avian orthoavulavirus 1; Newcastle disease; epidemiology; genetic variation; genomics; host range; indigenous species; monitoring; nucleotide sequences; phylogeny; poultry; poultry health; subgenotype; vaccines; virulent strains; viruses; wild birds; Nigeria
- Abstract:
- ... Newcastle disease virus (NDV) has a wide avian host range and a high degree of genetic variability, and virulent strains cause Newcastle disease (ND), a worldwide concern for poultry health. Although NDV has been studied in Nigeria, genetic information about the viruses involved in the endemicity of the disease and the transmission that likely occurs at the poultry-wildlife interface is still larg ...
- Handle:
- 10113/6480143
- DOI:
- 10.1007/s00705-019-04288-9
- CHORUS:
- 10.1007/s00705-019-04288-9
- https://doi.org/10.1007/s00705-019-04288-9
- « Previous
- Next »
- 1
- 2
- 3
- 4