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1. Virulence during Newcastle Disease Viruses Cross Species Adaptation

Author:
Claudio L. Afonso
Source:
Viruses 2021 v.13 no.1 pp. -
ISSN:
1999-4915
Subject:
Newcastle disease; adults; mutation; pathogenesis; pathotypes; phylogeny; virulence
Abstract:
... The hypothesis that host adaptation in virulent Newcastle disease viruses (NDV) has been accompanied by virulence modulation is reviewed here. Historical records, experimental data, and phylogenetic analyses from available GenBank sequences suggest that currently circulating NDVs emerged in the 1920–1940′s from low virulence viruses by mutation at the fusion protein cleavage site. These viruses la ...
DOI:
10.3390/v13010110

2. Genetic stability of a Newcastle disease virus vectored infectious laryngotracheitis virus vaccine after serial passages in chicken embryos

Author:
Claudio L. Afonso,   et al.   ; Qingzhong Yu; Yufeng Li; Kiril Dimitrov; Stephen Spatz; Laszlo Zsak;     Show all 6 Authors
Source:
Vaccine 2020 v.38 no.4 pp. 925-932
ISSN:
0264-410X
Subject:
Avian orthoavulavirus 1; Gallid alphaherpesvirus 1; chick embryos; chicken eggs; chickens; fluorescent antibody technique; genes; genetic stability; glycoproteins; growth models; high-throughput nucleotide sequencing; recombinant vaccines; reverse transcriptase polymerase chain reaction; single nucleotide polymorphism; virulence; viruses
Abstract:
... Previously, we have demonstrated that the recombinant Newcastle disease virus (NDV) expressing the infectious laryngotracheitis virus (ILTV) glycoprotein D (gD) conferred protection against both virulent NDV and ILTV challenges in chickens. In this study, we evaluated the genetic stability of the recombinant vaccine after eight serial passages in embryonated chicken eggs (ECE). The vaccine master ...
DOI:
10.1016/j.vaccine.2019.10.074

3. Runting and Stunting Syndrome in Broiler Chickens: Histopathology and Association With a Novel Picornavirus

Author:
Claudio L. Afonso,   et al.   ; Letícia B. de Oliveira; James B. Stanton; Jian Zhang; Corrie Brown; Salman Latif Butt; Kiril Dimitrov; Jeremy D. Volkening; Leonardo J. C. Lara; Camila Stefanie F. de Oliveira; Roselene Ecco;     Show all 11 Authors
Source:
Veterinary pathology 2021 v.58 no.1 pp. 123-135
ISSN:
1544-2217
Subject:
Avian nephritis virus; RNA; Rotavirus; animal pathology; atrophy; epidemiology; epithelium; etiology; genes; genetic similarity; histology; histopathology; hybridization; ileum; jejunum; pathogenesis; serosa
Abstract:
... Runting stunting syndrome (RSS) in commercial chickens has been reported worldwide, and although several studies have attempted to clarify the cause and describe the lesions, there are gaps in knowledge of the epidemiology, pathogenesis, and etiology. The study objective was to use commercial chicks naturally affected by RSS to describe the histologic changes of RSS in all segments of the small in ...
DOI:
10.1177/0300985820969971

4. Complete Genome Sequence of Avian Coronavirus Strain GA08 (GI-27 Lineage)

Author:
Claudio L. Afonso,   et al.   ; Iryna V. Goraichuk; James F. Davis; Arun B. Kulkarni; David L. Suarez;     Show all 5 Authors
Source:
Microbiology resource announcements 2020 v.9 no.9 pp. -
ISSN:
2576-098X
Subject:
Infectious bronchitis virus; broiler chickens; fecal examination; feces; infectious bronchitis; nucleotide sequences; viral genome; virulent strains; Georgia
Abstract:
... Avian coronavirus, also known as infectious bronchitis virus, is a highly contagious respiratory pathogen of chickens that is responsible for major economic losses to the poultry industry around the globe. Here, we report the complete genome sequence of strain GA08 of the GI-27 lineage, isolated from a fecal sample from a broiler chicken collected in Georgia in 2015. ...
Handle:
10113/7058196
DOI:
10.1128/MRA .00068-20

5. Surveillance and Genetic Characterization of Virulent Newcastle Disease Virus Subgenotype V.3 in Indigenous Chickens from Backyard Poultry Farms and Live Bird Markets in Kenya

Author:
Claudio L. Afonso,   et al.   ; Henry M. Kariithi; Helena L. Ferreira; Catharine N. Welch; Leonard O. Ateya; Auleria A. Apopo; Richard Zoller; Jeremy D. Volkening; Dawn Williams-Coplin; Darren J. Parris; Tim L. Olivier; Dana Goldenberg; Yatinder S. Binepal; Sonia M. Hernandez; David L. Suarez;     Show all 15 Authors
Source:
Viruses 2021 v.13 no.1 pp. -
ISSN:
1999-4915
Subject:
Avian orthoavulavirus 1; Newcastle disease; amino acids; chickens; farms; genome; monitoring; phylogeography; subgenotype; surveys; trade; virulence; viruses; Kenya
Abstract:
... Kenyan poultry consists of ~80% free-range indigenous chickens kept in small flocks (~30 birds) on backyard poultry farms (BPFs) and they are traded via live bird markets (LBMs). Newcastle disease virus (NDV) was detected in samples collected from chickens, wild farm birds, and other domestic poultry species during a 2017–2018 survey conducted at 66 BPFs and 21 LBMs in nine Kenyan counties. NDV nu ...
DOI:
10.3390/v13010103

6. Global phylodynamic analysis of avian paramyxovirus-1 provides evidence of inter-host transmission and intercontinental spatial diffusion

Author:
Claudio L. Afonso,   et al.   ; Joseph T. Hicks; Kiril M. Dimitrov; Andrew M. Ramey; Justin Bahl;     Show all 5 Authors
Source:
BMC evolutionary biology 2019 v.19 no. pp. 108
ISSN:
1471-2148
Subject:
Anseriformes; Avian orthoavulavirus 1; Bayesian theory; Columbiformes; Galliformes; Markov chain; Psittaciformes; ancestry; bird diseases; chickens; disease severity; geographical distribution; host range; host-pathogen relationships; nucleotide sequences; phylogeography; population structure; viral fusion proteins; virus transmission; wild birds; Africa; East Asia; Europe; Middle East; South Asia
Abstract:
... Avian avulavirus (commonly known as avian paramyxovirus-1 or APMV-1) can cause disease of varying severity in both domestic and wild birds. Understanding how viruses move among hosts and geography would be useful for informing prevention and control efforts. A Bayesian statistical framework was employed to estimate the evolutionary history of 1602 complete fusion gene APMV-1 sequences collected fr ...
Handle:
10113/7382946
DOI:
10.1186/s12862-019-1431-2

7. A retrospective study of Newcastle disease in Kenya

Author:
Claudio L. Afonso,   et al.   ; Auleria A. Apopo; Henry M. Kariithi; Leonard O. Ateya; Yatinder S. Binepal; Jane H. Sirya; Thomas D. Dulu; Catharine N. Welch; Sonia M. Hernandez;     Show all 9 Authors
Source:
Tropical animal health and production 2020 v.52 no.2 pp. 699-710
ISSN:
0049-4747
Subject:
Avian orthoavulavirus 1; Newcastle disease; agroecological zones; ambient temperature; animal viruses; chickens; climate; diagnostic techniques; farmers; indigenous species; introduced species; monitoring; mortality; poultry production; production technology; relative humidity; retrospective studies; Kenya
Abstract:
... Newcastle disease (ND) is a major constraint to Kenya’s poultry production, which is comprised of approximately 80% indigenous chickens (ICs; caged and free-range system) and 20% exotic chickens (intensive system). This study analyzed cases reported as suspected ND in Kenya between 2005 and 2015. Of the suspected 332 ND reported cases from the three production systems in 27 locations within six Ke ...
DOI:
10.1007/s11250-019-02059-x
PubMed:
31501991
PubMed Central:
PMC7039849

8. Virulent Newcastle disease viruses from chicken origin are more pathogenic and transmissible to chickens than viruses normally maintained in wild birds

Author:
Claudio L. Afonso,   et al.   ; Helena L. Ferreira; Tonya L. Taylor; Kiril M. Dimitrov; Mahmoud Sabra; David L. Suarez;     Show all 6 Authors
Source:
Veterinary microbiology 2019 v.235 pp. 25-34
ISSN:
0378-1135
Subject:
chickens; Newcastle disease; hosts; Avian orthoavulavirus 1; viruses; genotype; seroconversion; viral load; viral shedding; wild birds; virulence; mortality; signs and symptoms (animals and humans)
Abstract:
... Five, class II, virulent Newcastle disease virus (vNDV) isolates of different genotypes from different host species were evaluated for their ability to infect, cause disease, and transmit to naïve chickens. Groups of five birds received a low, medium, or high dose, by the oculonasal route, of one of the following vNDV: three chicken-origin, one cormorant-origin, and one pigeon-origin. Three naïve ...
Handle:
10113/6461958
DOI:
10.1016/j.vetmic.2019.06.004
CHORUS:
10.1016/j.vetmic.2019.06.004
Chorus Open Access:
10.1016/j.vetmic.2019.06.004

9. Genetic characterization and pathogenesis of the first H9N2 low pathogenic avian influenza viruses isolated from chickens in Kenyan live bird markets

Author:
Claudio L. Afonso,   et al.   ; Henry M. Kariithi; Catharine N. Welch; Helena L. Ferreira; Elizabeth A. Pusch; Leonard O. Ateya; Yatinder S. Binepal; Auleria A. Apopo; Thomas D. Dulu; David L. Suarez;     Show all 10 Authors
Source:
Infection, genetics, and evolution 2020 v.78 pp. 104074
ISSN:
1567-1348
Subject:
Influenza A virus; avian influenza; direct contact; evolution; infection; mammals; monitoring; mortality; nucleotide sequences; pathogenesis; poultry production; sequence analysis; seroconversion; viruses; zoonoses; Kenya
Abstract:
... Poultry production plays an important role in the economy and livelihoods of rural households in Kenya. As part of a surveillance program, avian influenza virus (AIV)-specific real-time RT-PCR (RRT-PCR) was used to screen 282 oropharyngeal swabs collected from chickens at six live bird markets (LBMs) and 33 backyard poultry farms in Kenya and 8 positive samples were detected. Virus was isolated in ...
DOI:
10.1016/j.meegid.2019.104074
CHORUS:
10.1016/j.meegid.2019.104074

10. Reverse genetics of Newcastle disease virus

Author:
Claudio L. Afonso,   et al.   ; Stivalis Cardenas-Garcia;     Show all 2 Author
Source:
Methods in Molecular Biology 2017 v.1602 no. pp. 141-158
ISSN:
1064-3745
Subject:
gene therapy; molecular biology; complementary DNA; bacteriophages; plasmids; Avian orthoavulavirus 1; genes; Vaccinia virus; vaccine development; site-directed mutagenesis; gene deletion; DNA-directed RNA polymerase
Abstract:
... Reverse genetics allows for the generation of recombinant viruses or vectors used in functional studies, vaccine development, and gene therapy. This technique enables genetic manipulation and cloning of viral genomes, gene mutation through site-directed mutagenesis, along with gene insertion or deletion, among other studies. An in vitro infection-based system including the highly attenuated vaccin ...
Handle:
10113/5763048
DOI:
10.1007/978-1-4939-6964-7_10
PubMed:
28508219
PubMed Central:
PMC7120584

11. Intracellular fixation buffer inactivates Newcastle disease virus in chicken allantoic fluid, macrophages and splenocytes

Author:
Claudio L. Afonso,   et al.   ; Valerie Marcano; Stivalis Cardenas-Garcia; Robert M. Gogal;     Show all 4 Authors
Source:
Journal of virological methods 2018 v.251 pp. 1-6
ISSN:
0166-0934
Subject:
chickens; phenotype; antigens; antibodies; allantoic fluid; heat; flow cytometry; ligands; macrophages; Avian orthoavulavirus 1; splenocytes; phosphates; formalin; ultraviolet radiation
Abstract:
... Inactivation of Newcastle disease virus (NDV) has been routinely achieved with heat, β-propiolactone, binary ethylenimine, ultraviolet light and formalin. However, these strategies have not been tested for cell surface ligand or receptor phenotype in viral-infected chicken immune cells. To study the capacity of fixation buffers to preserve surface markers while inactivating NDV, a primary splenocy ...
Handle:
10113/5838019
DOI:
10.1016/j.jviromet.2017.09.025

12. First Complete Genome Sequences of a Subgenotype Vd Newcastle Disease Virus Isolate

Author:
Claudio L. Afonso,   et al.   ; Iryna V. Goraichuk; Peter L. M. Msoffe; Gaspar H. Chiwanga; Kiril M. Dimitrov; David L. Suarez;     Show all 6 Authors
Source:
Microbiology resource announcements 2019 v.8 no.27 pp. -
ISSN:
2576-098X
Subject:
Avian orthoavulavirus 1; chickens; live bird markets; nucleotide sequences; subgenotype; viral genome; Tanzania
Abstract:
... A Newcastle disease virus was isolated from a chicken from a live birdmarket in the Mbeya region of Tanzania. Complete genome characterization of the isolate identified it as a member of subgenotype Vd. This is the first complete genome sequence of this subgenotype. ...
Handle:
10113/6837728
DOI:
10.1128/MRA.00436-19
PubMed:
31270191
PubMed Central:
PMC6606905

13. MinION sequencing to genotype US strains of infectious laryngotracheitis virus

Author:
Claudio L. Afonso,   et al.   ; Stephen J. Spatz; Maricarmen Garcia; Sylva Riblet; Teresa A. Ross; Jeremy D. Volkening; Tonya L. Taylor; Taejoong Kim;     Show all 8 Authors
Source:
Avian pathology 2019 v.48 no.3 pp. 255-269
ISSN:
1465-3338
Subject:
restriction fragment length polymorphism; allelic variation; Gallid alphaherpesvirus 1; nanopores; alleles; monitoring; open reading frames; flocks; industry; polymerase chain reaction; phylogeny; genotype; genotyping; virulence; birds; single nucleotide polymorphism; United States
Abstract:
... Over the last decade the US broiler industry has fought long-lasting outbreaks of infectious laryngotracheitis (ILTV). Previously, nine genotypes (I-IX) of ILTVs have been recognized using the polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) method with three viral alleles (gB, gM and UL47/gG). In this study, the genotyping system was simplified to six genotypes by am ...
Handle:
10113/6372540
DOI:
10.1080/03079457.2019.1579298

14. First Genome Sequence of Newcastle Disease Virus of Genotype VIIi from Jordan

Author:
Claudio L. Afonso,   et al.   ; Mustafa Ababneh; Helena L. Ferreira; Mohammad Khalifeh; David L. Suarez;     Show all 5 Authors
Source:
Microbiology resource announcements 2018 v.7 no.23 pp. e01136
ISSN:
2576-098X
Subject:
Avian orthoavulavirus 1; RNA; chickens; molecular systematics; nucleotide sequences; phylogeny; spleen; subgenotype; viral genome; Jordan
Abstract:
... Newcastle disease virus (NDV) was detected by reverse transcriptase PCR (RT-PCR) from total RNA isolated from a chicken spleen of a backyard flock in Jordan. The complete coding genome sequence of NDV/chicken/Jordan/J11-spleen/2018 was obtained with MiSeq (Illumina) sequencing. Phylogenetic analysis of the concatenated coding sequences classified the virus as class II subgenotype VIIi. ...
Handle:
10113/7382910
DOI:
10.1128/MRA.01136-18

15. Genome-wide analysis reveals class and gene specific codon usage adaptation in avian paramyxoviruses 1

Author:
Claudio L. Afonso,   et al.   ; Tonya L. Taylor; Kiril M. Dimitrov;     Show all 3 Authors
Source:
Infection, genetics, and evolution 2017 v.50 pp. 28-37
ISSN:
1567-1348
Subject:
Gallus gallus; transcription (genetics); codons; Avian orthoavulavirus 1; viruses; genotype; birds
Abstract:
... In order to characterize the evolutionary adaptations of avian paramyxovirus 1 (APMV-1) genomes, we have compared codon usage and codon adaptation indexes among groups of Newcastle disease viruses that differ in biological, ecological, and genetic characteristics. We have used available GenBank complete genome sequences, and compared codon usage of class I (CI-29 sequences containing 132,675 codon ...
DOI:
10.1016/j.meegid.2017.02.004
CHORUS:
10.1016/j.meegid.2017.02.004
Chorus Open Access:
10.1016/j.meegid.2017.02.004

16. Experimental Infection and Transmission of Newcastle Disease Vaccine Virus in Four Wild Passerines

Author:
Claudio L. Afonso,   et al.   ; Andrea J. Ayala; Sonia M. Hernandez; Timothy L. Olivier; Catherine N. Welch; Kiril M. Dimitrov; Iryna V. Goraichuk; Patti J. Miller;     Show all 8 Authors
Source:
Avian diseases 2019 v.63 no.3 pp. 389-399
ISSN:
0005-2086
Subject:
Avian orthoavulavirus 1; Cardinalis cardinalis; Carpodacus mexicanus; Molothrus ater; Newcastle disease; White Leghorn; adults; antibodies; antibody formation; blood; chickens; cloaca; drinking water; hemagglutination; live vaccines; monitoring; reverse transcriptase polymerase chain reaction; signs and symptoms (animals and humans); specific pathogen-free animals; viral shedding; viruses; wild birds
Abstract:
... Our prior work has shown that live poultry vaccines have been intermittently isolated from wild birds sampled during field surveillance studies for Newcastle disease virus (NDV). Thus, we experimentally investigated the susceptibility of four native agriculturally associated wild bird species to the NDV LaSota vaccine and evaluated the shedding dynamics, potential transmission from chickens, and h ...
Handle:
10113/6743214
DOI:
10.1637/11980-092918-Reg.1

17. Enhanced phylogenetic resolution of Newcastle disease outbreaks using complete viral genome sequences from formalin-fixed paraffin-embedded tissue samples

Author:
Claudio L. Afonso,   et al.   ; Salman Latif Butt; Kiril M. Dimitrov; Jian Zhang; Abdul Wajid; Tasra Bibi; Asma Basharat; Corrie C. Brown; Shafqat F. Rehmani; James B. Stanton;     Show all 10 Authors
Source:
Virus genes 2019 v.55 no.4 pp. 502-512
ISSN:
0920-8569
Subject:
Avian orthoavulavirus 1; Newcastle disease; RNA; brain; chickens; data analysis; disease control; disease outbreaks; epidemiological studies; flocks; genes; high-throughput nucleotide sequencing; intestines; lungs; nucleotide sequences; phylogeny; poultry production; spleen; subgenotype; tissues; virulence; viruses; Pakistan
Abstract:
... Highly virulent Newcastle disease virus (NDV) causes Newcastle disease (ND), which is a threat to poultry production worldwide. Effective disease management requires approaches to accurately determine sources of infection, which involves tracking of closely related viruses. Next-generation sequencing (NGS) has emerged as a research tool for thorough genetic characterization of infectious organisms ...
DOI:
10.1007/s11262-019-01669-9

18. Newcastle disease vaccines—A solved problem or a continuous challenge?

Author:
Claudio L. Afonso,   et al.   ; Kiril M. Dimitrov; Qingzhong Yu; Patti J. Miller;     Show all 4 Authors
Source:
Veterinary microbiology 2017 v.206 pp. 126-136
ISSN:
0378-1135
Subject:
chickens; neutralization; Newcastle disease; research and development; biosecurity; antigens; gastrointestinal system; inactivated vaccines; antibodies; flocks; animal health; Avian orthoavulavirus 1; villages; immunization; death; virulence; mortality; virulent strains
Abstract:
... Newcastle disease (ND) has been defined by the World Organisation for Animal Health as infection of poultry with virulent strains of Newcastle disease virus (NDV). Lesions affecting the neurological, gastrointestinal, respiratory, and reproductive systems are most often observed. The control of ND must include strict biosecurity that prevents virulent NDV from contacting poultry, and also proper a ...
Handle:
10113/5734383
DOI:
10.1016/j.vetmic.2016.12.019
PubMed:
28024856
PubMed Central:
PMC7131810

19. Detection of Fowlpox virus carrying distinct genome segments of Reticuloendotheliosis virus

Author:
Claudio L. Afonso,   et al.   ; Lok R. Joshi; Fernando V. Bauermann; Kyle S. Hain; Gerald F. Kutish; Anibal G. Armién; Chad P. Lehman; Regg Neiger; Deoki N. Tripathy; Diego G. Diel;     Show all 10 Authors
Source:
Virus research 2019 v.260 pp. 53-59
ISSN:
0168-1702
Subject:
Fowlpox virus; Meleagris gallopavo; Reticuloendotheliosis virus; chickens; fowl pox; genome; head; homologous recombination; metagenomics; nucleic acids; polymerase chain reaction; proviruses; sequence analysis; skin lesions; terminal repeat sequences; transmission electron microscopy; turkeys; virion; United States
Abstract:
... Fowlpox virus (FWPV), the type species of the genus Avipoxvirus family Poxviridae, is a large double-stranded DNA virus that causes fowlpox in chickens and turkeys. Notably, sequences of the avian retrovirus reticuloendotheliosis virus (REV) are frequently found integrated into the genome of FWPV. While some FWPV strains carry remnants of the REV long terminal repeats (LTRs), other strains have be ...
Handle:
10113/6195913
DOI:
10.1016/j.virusres.2018.10.017
CHORUS:
10.1016/j.virusres.2018.10.017

20. Tropism of Newcastle disease virus strains for chicken neurons, astrocytes, oligodendrocytes, and microglia

Author:
Claudio L. Afonso,   et al.   ; Salman L. Butt; Veridiana Maria Brianezi Dignani Moura; Leonardo Susta; Patti J. Miller; Jessica M. Hutcheson; Stivalis Cardenas-Garcia; Corrie C. Brown; Franklin D. West; James B. Stanton;     Show all 10 Authors
Source:
BMC veterinary research 2019 v.15 no.1 pp. 317
ISSN:
1746-6148
Subject:
Avian orthoavulavirus 1; Newcastle disease; White Leghorn; astrocytes; brain; chickens; chicks; encephalitis; fluorescent antibody technique; neurons; nucleoproteins; oligodendroglia; parenchyma (animal tissue); pathotypes; phenotype; signs and symptoms (animals and humans); virulence; virulent strains
Abstract:
... BACKGROUND: Newcastle disease (ND), which is caused by infections of poultry species with virulent strains of Avian orthoavulavirus-1, also known as avian paramyxovirus 1 (APMV-1), and formerly known as Newcastle disease virus (NDV), may cause neurological signs and encephalitis. Neurological signs are often the only clinical signs observed in birds infected with neurotropic strains of NDV. Experi ...
DOI:
10.1186/s12917-019-2053-z
PubMed:
31484573
PubMed Central:
PMC6727330
CHORUS:
10.1186/s12917-019-2053-z