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1. pCP13, a representative of a new family of conjugative toxin plasmids in Clostridium perfringens

Author:
Watts, Thomas D.; Vidor, Callum J.; Awad, Milena M.; Lyras, Dena; Rood, Julian I.; Adams, Vicki
Source:
Plasmid 2019 v.102 pp. 37-45
ISSN:
0147-619X
Subject:
Clostridium botulinum; Clostridium perfringens,   etc   ; animal pathogens; antibiotic resistance; bacteria; bioinformatics; enterotoxins; genes; humans; loci; plasmids; virulence;     Show all 12 Subjects
Abstract:
... Conjugative transfer is a major contributor to the dissemination of antibiotic resistance and virulence genes in the human and animal pathogen, Clostridium perfringens. The C. perfringens plasmid pCW3 is the archetype of an extensive family of highly related conjugative toxin and antibiotic resistance plasmids found in this bacterium. These plasmids were thought to constitute the only conjugative ...
DOI:
10.1016/j.plasmid.2019.02.002

2. Detection and molecular characterization of Clostridium difficile ST 1 in Buenos Aires, Argentina

Author:
Cejas, Daniela; Ríos Osorio, Néstor Raúl; Quirós, Rodolfo; Sadorin, Roxana; Berger, María Alejandra; Gutkind, Gabriel; Fernández Canigia, Liliana; Radice, Marcela
Source:
Anaerobe 2018 v.49 pp. 14-17
ISSN:
1075-9964
Subject:
Clostridium difficile,   etc   ; genes; Argentina;     Show all 3 Subjects
Abstract:
... Thirty one C. difficile isolates recovered in 2015 were characterized. Nineteen/31 were positive for tcdA/B, among them, 4 isolates were also positive for CDT coding genes. Two/4 cdtA/B positives isolates corresponded to ST 1 resembling BI/NAP1/027/ST 1 strain, while the others corresponded to ST 226 and ST 377. ...
DOI:
10.1016/j.anaerobe.2017.10.007

3. Acetone-free biobutanol production: Past and recent advances in the Isopropanol-Butanol-Ethanol (IBE) fermentation

Author:
dos Santos Vieira, Carla Ferreira; Maugeri Filho, Francisco; Maciel Filho, Rubens; Pinto Mariano, Adriano
Source:
Bioresource technology 2019 v.287 pp. 121425
ISSN:
0960-8524
Subject:
Clostridium,   etc   ; acetone; biobutanol; butanol; feedstocks; fermentation; isopropyl alcohol; microorganisms; tanks;     Show all 9 Subjects
Abstract:
... Production of butanol for fuel via the conventional Acetone-Butanol-Ethanol fermentation has been considered economically risky because of a potential oversupply of acetone. Alternatively, acetone is converted into isopropanol by specific solventogenic Clostridium species in the Isopropanol-Butanol-Ethanol (IBE) fermentation. This route, although less efficient, has been gaining attention because ...
DOI:
10.1016/j.biortech.2019.121425

4. Highly efficient continuous acetone–butanol–ethanol production from mixed sugars without carbon catabolite repression

Author:
Kihara, Takahiro; Noguchi, Takuya; Tashiro, Yukihiro; Sakai, Kenji; Sonomoto, Kenji
Source:
Bioresource technology reports 2019 v.7 pp. 100185
ISSN:
2589-014X
Subject:
Clostridium saccharoperbutylacetonicum,   etc   ; catabolite repression; cellobiose; continuous fermentation; glucose; recycling; xylose;     Show all 7 Subjects
Abstract:
... Factors including the composition and concentration of mixed sugars, dilution rate, and cell density were examined to establish a highly efficient continuous acetone–butanol–ethanol (ABE) fermentation process using Clostridium saccharoperbutylacetonicum N1-4. During conventional continuous fermentation at a dilution rate of 0.15 h−1, the glucose/xylose mixture exhibited carbon catabolite repressio ...
DOI:
10.1016/j.biteb.2019.03.017

5. Random mutagenesis of Clostridium butyricum strain and optimization of biosynthesis process for enhanced production of 1,3-propanediol

Author:
Yang, Miaomiao; An, Yingfeng; Zabed, Hossain M.; Guo, Qi; Yun, Junhua; Zhang, Guoyan; Awad, Faisal N.; Sun, Wenjing; Qi, Xianghui
Source:
Bioresource technology 2019 v.284 pp. 188-196
ISSN:
0960-8524
Subject:
Clostridium butyricum,   etc   ; ambient temperature; biosynthesis; mutagenesis; mutants; response surface methodology;     Show all 6 Subjects
Abstract:
... The aim of this work was to study the random mutagenesis of Clostridium butyricum strain. A high 1,3-PD tolerant mutant strain, designated as C. butyricum YP855, was developed from the wild strain C. butyricum XYB11, using combined chemical (NTG, N-methyl-N′-nitro-N-nitrosoguanidine,) and plasma-based mutagenesis (ARTP, atmospheric and room temperature plasma). The YP855 showed a maximum tolerance ...
DOI:
10.1016/j.biortech.2019.03.098

6. Clostridium septicum arthritis in a young infant: A case report

Author:
Grover, Pragati; Bala, Kiran; Muralidharan, Jayashree; Angrup, Archana; Ray, Pallab
Source:
Anaerobe 2019 v.57 pp. 32-34
ISSN:
1075-9964
Subject:
Clostridium septicum,   etc   ; anaerobes; arthritis; bacteria; case studies; childhood; infancy; neonates;     Show all 8 Subjects
Abstract:
... Septic arthritis is one of the most serious conditions leading to grave consequences in all age groups, with a number of cases seen in infancy and childhood. Although septic arthritis in neonates is a known clinical entity, it is a diagnostic dilemma for the paediatricians. Of the reported causes of bacterial arthritis, anaerobic bacteria account for only 1%. Anaerobic bacterial septic arthritis m ...
DOI:
10.1016/j.anaerobe.2019.03.009

7. A rapid and sensitive enzymatic assay for 2,3-butanediol

Author:
Lee, Gyu Bi; Kim, Yun Jae; Lim, Jae Kyu; Kim, Tae Wan; Kang, Sung Gyun; Lee, Jung-Hyun; Lee, Hyun Sook
Source:
3 Biotech 2019 v.9 no.5 pp. 170
ISSN:
2190-572X
Subject:
Clostridium ljungdahlii,   etc   ; enzymes; high performance liquid chromatography; microorganisms; screening;     Show all 5 Subjects
Abstract:
... In this study, we developed a rapid and sensitive enzymatic assay for 2,3-butanediol (2,3-BDO) detection. The concentration of 2,3-BDO was determined by measuring the reduction of NADP⁺ using Clostridium ljungdahlii 2,3-butanediol dehydrogenase (CL-Bdh). The enzymatic assay could detect as low as 0.01 mM of 2,3-BDO, while the high-performance liquid chromatography (HPLC) method required a much hig ...
DOI:
10.1007/s13205-019-1705-9

8. Fecal calprotectin and fecal indole predict outcome of fecal microbiota transplantation in subjects with recurrent Clostridium difficile infection

Author:
Hibbard, J.; Jiang, Z.-D.; DuPont, H.L.
Source:
Anaerobe 2019 v.56 pp. 102-105
ISSN:
1075-9964
Subject:
Clostridium difficile,   etc   ; immunologic factors; intestinal microorganisms; nutritional status; prognosis;     Show all 5 Subjects
Abstract:
... Fecal calprotectin and indole were studied in 134 subjects with recurrent CDI before and after FMT. Reduced fecal calprotectin (p = 0.0353, 95% CI 0.1305–0.1439) and rising levels of indole (p < 0.0001, 95% CI < 0.0001–0.0003) predicted successful treatment. A ratio of recal calprotectin/indole may provide prognostic value for FMT (p = 0.0004, 95% CI 0.22–0.87). ...
DOI:
10.1016/j.anaerobe.2019.03.006

9. Native joint septic arthritis due to Clostridium tarantellae

Author:
Cointe, Aurélie; de Ponfilly, Gauthier Péan; Munier, Anne-Lise; Bachir, Marwa; Benmansour, Hanaa; Crémieux, Anne-Claude; Forien, Marine; Frazier, Aline; Krief, Elie; Cambau, Emmanuelle; Jacquier, Hervé
Source:
Anaerobe 2019 v.56 pp. 46-48
ISSN:
1075-9964
Subject:
Clostridium,   etc   ; Eubacterium tarantellae; arthritis; infectious diseases; patients; therapeutics;     Show all 6 Subjects
Abstract:
... Clostridium is a diverse genus including more than 200 species involved in varied clinical presentations in infectious diseases. Septic arthritis caused by Clostridium sp. are however uncommon. We report here the first septic arthritis due to Clostridium tarantellae, formerly called Eubacterium tarantellae, in a patient under anti-TNF therapy. ...
DOI:
10.1016/j.anaerobe.2019.02.004

10. Structural insight into Wnt signaling inhibition by Clostridium difficile toxin B

Author:
Chen, Peng; Tao, Liang; Liu, Zheng; Dong, Min; Jin, Rongsheng
Source:
TheFEBS journal 2019 v.286 no.5 pp. 874-881
ISSN:
1742-464X
Subject:
Clostridium difficile,   etc   ; epithelium; humans; morbidity; mortality; pathogenesis; receptors; therapeutics; virulence;     Show all 9 Subjects
Abstract:
... The incidence of Clostridium difficile infection (CDI) has increased significantly worldwide, causing substantial morbidity and mortality. One of the major virulence factor, TcdB, manages to enter the colonic epithelia via the human frizzled proteins (FZDs), which are physiological receptors for Wnt morphogens. Binding of TcdB to FZDs inhibits Wnt signaling, which may contribute to pathogenesis of ...
DOI:
10.1111/febs.14681

11. The Bacterial Chromatin Protein HupA Can Remodel DNA and Associates with the Nucleoid in Clostridium difficile

Author:
Oliveira Paiva, Ana M.; Friggen, Annemieke H.; Qin, Liang; Douwes, Roxanne; Dame, Remus T.; Smits, Wiep Klaas
Source:
Journal of molecular biology 2019 v.431 no.4 pp. 653-672
ISSN:
0022-2836
Subject:
Clostridium difficile,   etc   ; DNA; Escherichia coli; bacteria; chromatin; metabolism;     Show all 6 Subjects
Abstract:
... The maintenance and organization of the chromosome plays an important role in the development and survival of bacteria. Bacterial chromatin proteins are architectural proteins that bind DNA and modulate its conformation, and by doing so affect a variety of cellular processes. No bacterial chromatin proteins of Clostridium difficile have been characterized to date.Here, we investigate aspects of th ...
DOI:
10.1016/j.jmb.2019.01.001

12. Clostridial whole cell and enzyme systems for hydrogen production: current state and perspectives

Author:
Latifi, Amel; Avilan, Luisana; Brugna, Myriam
Source:
Applied microbiology and biotechnology 2019 v.103 no.2 pp. 567-575
ISSN:
0175-7598
Subject:
Clostridium,   etc   ; agricultural wastes; anaerobes; bacteria; fermentation; hydrogen production; industrial wastes;     Show all 7 Subjects
Abstract:
... Strictly anaerobic bacteria of the Clostridium genus have attracted great interest as potential cell factories for molecular hydrogen production purposes. In addition to being a useful approach to this process, dark fermentation has the advantage of using the degradation of cheap agricultural residues and industrial wastes for molecular hydrogen production. However, many improvements are still req ...
DOI:
10.1007/s00253-018-9514-3

13. n-Butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases

Author:
Bao, Teng; Zhao, Jingbo; Li, Jing; Liu, Xin; Yang, Shang-Tian
Source:
Bioresource technology 2019 v.285 pp. 121316
ISSN:
0960-8524
Subject:
Clostridium acetobutylicum; Clostridium cellulovorans,   etc   ; NADP (coenzyme); alcohol dehydrogenase; biosynthesis; butanol; butyric acid; cellulose; ethanol; ethanol production; fermentation; glucose; metabolic engineering;     Show all 13 Subjects
Abstract:
... With high cellulolytic and acetic/butyric acids production abilities, Clostridium cellulovorans is promising for use to produce cellulosic n-butanol. Here, we introduced three different aldehyde/alcohol dehydrogenases encoded by bdhB, adhE1, and adhE2 from Clostridium acetobutylicum into C. cellulovorans and studied their effects on ethanol and n-butanol production. Compared to AdhE2, AdhE1 was mo ...
DOI:
10.1016/j.biortech.2019.121316

14. Production of 1,3-propanediol from pure and crude glycerol using a UASB reactor with attached biomass in silicone support

Author:
Veras, S.T.S.; Rojas, P.; Florencio, L.; Kato, M.T.; Sanz, J.L.
Source:
Bioresource technology 2019 v.279 pp. 140-148
ISSN:
0960-8524
Subject:
Clostridium beijerinckii; Clostridium pasteurianum,   etc   ; Klebsiella pneumoniae; Lactobacillus; biofilm; biomass; cost effectiveness; glycerol; microorganisms; pH; silicone; upflow anaerobic sludge blanket reactor;     Show all 12 Subjects
Abstract:
... The 1,3-propanediol (1,3-PDO) yield and productivity from glycerol were studied over a 155-day period. A UASB reactor that also contained silicone support for biomass attachment was used to evaluate the optimal operational conditions and microbiota development. The highest average 1,3-PDO yield was 0.54 and 0.48 mol.mol-gly−1 when reactor pH was 5.0–5.5 and the applied loading rate was 18 and 20 g ...
DOI:
10.1016/j.biortech.2019.01.125

15. Production of (S)-1,2-propanediol from l-rhamnose using the moderately thermophilic Clostridium strain AK1

Author:
Ingvadottir, Eva Maria; Scully, Sean M.; Orlygsson, Johann
Source:
Anaerobe 2018 v.54 pp. 26-30
ISSN:
1075-9964
Subject:
Clostridium,   etc   ; fucose; pH; rhamnose; temperature;     Show all 5 Subjects
Abstract:
... Clostridium strain AK1 was investigated for its capacity of producing 1,2-propanediol from l-rhamnose but not l-fucose. The maximum yields of 1,2-propanediol from rhamnose was 0.81 mol 1,2-PD/mol l-rhamnose. The influence of different initial substrate concentrations as well as the effect of temperature and pH on 1,2-PD production was investigated. ...
DOI:
10.1016/j.anaerobe.2018.07.003

16. Phage serine integrase-mediated genome engineering for efficient expression of chemical biosynthetic pathway in gas-fermenting Clostridium ljungdahlii

Author:
Huang, He; Chai, Changsheng; Yang, Sheng; Jiang, Weihong; Gu, Yang
Source:
Metabolic engineering 2019 v.52 pp. 293-302
ISSN:
1096-7176
Subject:
Clostridium acetobutylicum; Clostridium difficile; Clostridium ljungdahlii,   etc   ; DNA fragmentation; Streptomyces; acetogens; anaerobes; bacteriophages; biochemical pathways; butyric acid; carbon dioxide; carbon monoxide; enzymes; fuels; genes; genetic engineering; genetic stability; serine; synthesis gas;     Show all 19 Subjects
Abstract:
... The real value of gas-fermenting clostridia, capable of using CO and CO2, resides in their potential of being developed into cell factories to produce various bulk chemicals and fuels. This process requires rapid chromosomal integration of heterologous chemical biosynthetic pathways, which is impeded by the absence of genetic tools competent for efficient genome engineering in these anaerobes. Her ...
DOI:
10.1016/j.ymben.2019.01.005

17. The alteration of gut microbiota in newly diagnosed type 2 diabetic patients

Author:
Chen, Pei-Chi; Chien, Yi-Wen; Yang, Suh-Ching
Source:
Nutrition 2019 v.63-64 pp. 51-56
ISSN:
0899-9007
Subject:
Clostridium coccoides; Clostridium leptum,   etc   ; Lactobacillus; blood sampling; dysbiosis; enzymes; feces; food records; glucose; glycohemoglobin; intestinal microorganisms; models; noninsulin-dependent diabetes mellitus; patients; ribosomal RNA;     Show all 15 Subjects
Abstract:
... Gut microbiota dysbiosis is known to be associated with diabetes; however, the findings of previous studies are conflicting. To clarify the association between type 2 diabetes and the gut microbiota, the present study analyzed the composition of fecal gut microbiota and its correlation with specific clinical parameters in newly diagnosed, treatment-naive diabetic patients and healthy controls.A to ...
DOI:
10.1016/j.nut.2018.11.019

18. Development of an interspecies interaction model: An experiment on Clostridium cadaveris and Clostridium sporogenes under anaerobic condition

Author:
Koo, Taewoan; Lee, Joonyeob; Hwang, Seokhwan
Source:
Journal of environmental management 2019 v.237 pp. 247-254
ISSN:
0301-4797
Subject:
Clostridium cadaveris; Clostridium sporogenes,   etc   ; anaerobic digestion; carbon; glucose; interspecific competition; microbial growth; mixed culture; models; quantitative polymerase chain reaction; specific growth rate; wastewater treatment;     Show all 12 Subjects
Abstract:
... The specific primer and probe sets for quantifying Clostridium cadaveris and Clostridium sporogenes using a quantitative real-time PCR were designed. Each primer and probe set detected only the target species very specifically. The two species were cultivated in pure and mixed culture in batch mode with glucose as the only carbon source. The designed QPCR sets were used successfully to estimate th ...
DOI:
10.1016/j.jenvman.2019.02.084

19. Butanol and butyric acid production from Saccharina japonica by Clostridium acetobutylicum and Clostridium tyrobutyricum with adaptive evolution

Author:
Ra, Chae Hun; Sunwoo, In Yung; Nguyen, Trung Hau; Sukwang, Pailin; Sirisuk, Phunlap; Jeong, Gwi-Taek; Kim, Sung-Koo
Source:
Bioprocess and biosystems engineering 2019 v.42 no.4 pp. 583-592
ISSN:
1615-7591
Subject:
Clostridium acetobutylicum; Clostridium tyrobutyricum,   etc   ; Saccharina japonica; acid hydrolysis; butanol; butyric acid; enzymes; evolutionary adaptation; hydrolysates; macroalgae; monosaccharides; potassium dihydrogen phosphate; saccharification; slurries; sodium bicarbonate; sulfuric acid;     Show all 16 Subjects
Abstract:
... Optimal conditions of hyper thermal (HT) acid hydrolysis of the Saccharina japonica was determined to a seaweed slurry content of 12% (w/v) and 144 mM H₂SO₄ at 160 °C for 10 min. Enzymatic saccharification was carried out at 50 °C and 150 rpm for 48 h using the three enzymes at concentrations of 16 U/mL. Celluclast 1.5 L showed the lowest half-velocity constant (Kₘ) of 0.168 g/L, indicating a high ...
DOI:
10.1007/s00449-018-02063-9

20. Engineer multi-functional cellulase/xylanase/β-glucosidase with improved efficacy to degrade rice straw

Author:
Chen, Chun-Hsu; Yao, Jyun-Yu; Yang, Barbara; Lee, Hsiao-Lin; Yuan, Shuo-Fu; Hsieh, Han-Yu; Liang, Po-Huang
Source:
Bioresource technology reports 2019 v.5 pp. 170-177
ISSN:
2589-014X
Subject:
Clostridium cellulovorans; Clostridium thermocellum,   etc   ; beta-glucosidase; biofuels; biomass; carboxymethylcellulose; cell growth; cell walls; cellobiose; endo-1,4-beta-glucanase; glucose; lignocellulose; polymers; rice straw; xylan; xylanases; xylose; yeasts;     Show all 18 Subjects
Abstract:
... Plant lignocellulosic biomass can be enzymatically degraded and converted into biofuel. We have studied a bifunctional cellulase/xylanase from thermophilic Clostridium thermocellum (CtCel5E), which can digest two major plant cell wall polysaccharides, cellulose (a polymer of glucose) and xylan (a major hemicellulose composed of xylose), into disaccharides cellobiose and xylobiose, respectively. In ...
DOI:
10.1016/j.biteb.2019.01.008