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- Author:
- Santoro, Azzurra; Dorbek-Kolin, Elisabeth; Jeremejeva, Julia; Tummeleht, Lea; Orro, Toomas; Jokelainen, Pikka; Lassen, Brian
- Source:
- Parasitology 2019 v.146 no.2 pp. 261-267
- ISSN:
- 1469-8161
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium parvum; Cryptosporidium ryanae; DNA; calves; farms; feces; genes; glycoproteins; livestock and meat industry; molecular epidemiology; parasites; questionnaires; ribosomal RNA; spring; Estonia; Show all 16 Subjects
- Abstract:
- ... We investigated the molecular epidemiology of Cryptosporidium spp. in Estonia by testing fecal samples from 486 calves aged <2 months, raised on 53 cattle farms, for the presence of Cryptosporidium DNA. The parasites were identified and characterized by sequencing of the 18S rRNA gene and of the 60 kDa glycoprotein (gp60) gene. Moreover, using a questionnaire, we surveyed factors that could be rel ...
- DOI:
- 10.1017/S0031182018001348
- PubMed:
- 30086806 30086806
- http://dx.doi.org/10.1017/S0031182018001348
- Author:
- Li, Na; Wang, Rui; Cai, Min; Jiang, Wen; Feng, Yaoyu; Xiao, Lihua
- Source:
- International journal for parasitology 2019 v.49 no.7 pp. 569-577
- ISSN:
- 0020-7519
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium andersoni; Cryptosporidium parvum; Cryptosporidium ryanae; Rotavirus; antibiotics; cryptosporidiosis; dairy calves; dairy farming; diarrhea; enteropathogens; farms; feces; genotyping; immunoassays; mixed infection; polymerase chain reaction; regression analysis; risk factors; therapeutics; virulence; China; Show all 22 Subjects
- Abstract:
- ... Neonatal diarrhea is one of the most important syndromes in dairy cattle. Among enteropathogens, Cryptosporidium spp. are primary causes of diarrhea, but outbreaks due to cryptosporidiosis are rarely reported in cattle. From January to April in 2016, severe diarrhea was observed in over 400 neonatal dairy calves on a large dairy farm in Jiangsu Province of East China. Approximately 360 calves died ...
- DOI:
- 10.1016/j.ijpara.2019.02.006
- https://dx.doi.org/10.1016/j.ijpara.2019.02.006
3. Molecular epidemiology of Cryptosporidium spp. in dairy cattle in Guangdong Province, South China
- Author:
- Liang, Nan; Wu, Yayun; Sun, Mingfei; Chang, Yankai; Lin, Xuhui; Yu, Linzeng; Hu, Suhui; Zhang, Xiangqian; Zheng, Shuangjian; Cui, Zhaohui; Zhang, Longxian
- Source:
- Parasitology 2019 v.146 no.1 pp. 28-32
- ISSN:
- 1469-8161
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium andersoni; Cryptosporidium parvum; Cryptosporidium ryanae; DNA; adults; calves; dairy cattle; dairy farming; farms; feces; heifers; linkage disequilibrium; molecular epidemiology; multilocus sequence typing; nucleotide sequences; polymerase chain reaction; population structure; sheep; China; Show all 20 Subjects
- Abstract:
- ... To determine the prevalence of Cryptosporidium in dairy cattle in Guangdong Province, South China, 1440 fecal samples were collected from 10 farms and screened for Cryptosporidium with PCR. The overall prevalence of Cryptosporidium was 4.38% (63/1440), and the infection rates in preweaned calves, postweaned calves, heifers and adults were 6.4% (19/297), 6.19% (33/533), 1.48% (4/271) and 2.06% (7/3 ...
- DOI:
- 10.1017/S0031182018001129
- PubMed:
- 29986775
- http://dx.doi.org/10.1017/S0031182018001129
- Author:
- Wang, Geping; Wang, Guanghua; Li, Xiuping; Zhang, Xueyong; Karanis, Gabriele; Jian, Yingna; Ma, Liqing; Karanis, Panagiotis
- Source:
- Parasitology research 2018 v.117 no.6 pp. 1793-1800
- ISSN:
- 0932-0113
- Subject:
- Cryptosporidium bovis, etc ; Giardia lamblia; diarrhea; feces; fluorescence microscopy; fluorescent antibody technique; humans; parasites; polymerase chain reaction; staining; towns; yaks; China; Show all 13 Subjects
- Abstract:
- ... Cryptosporidium and Giardia are ubiquitous parasites that infect humans and animals. Few reports are available on the prevalence of these two protozoan parasites in yaks (Bos grunniens). In this study, 344 faecal samples were collected from yaks with diarrhoea in the Chenduo and Nangqian counties of Qinghai Province, China. Cryptosporidium spp. and Giardia duodenalis were detected by light and imm ...
- DOI:
- 10.1007/s00436-018-5861-3
- http://dx.doi.org/10.1007/s00436-018-5861-3
- Author:
- Feng, Yuanyuan; Gong, Xiaoqing; Zhu, Kexin; Li, Na; Yu, Zhengjie; Guo, Yaqiong; Weng, Yabiao; Kváč, Martin; Feng, Yaoyu; Xiao, Lihua
- Source:
- Parasites & vectors 2019 v.12 no.1 pp. 41
- ISSN:
- 1756-3305
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium parvum; Cryptosporidium ryanae; Enterocytozoon bieneusi; Giardia lamblia; chi-square distribution; climate; dairy calves; disease transmission; dominant species; emerging diseases; enteropathogens; genotype; genotyping; humans; loci; monsoon season; polymerase chain reaction; sequence analysis; signs and symptoms (animals and humans); spring; summer; winter; zoonoses; China; Show all 25 Subjects
- Abstract:
- ... BACKGROUND: Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi are common enteric pathogens in humans and animals. Data on the transmission of these pathogens are scarce from Guangdong, China, which has a subtropical monsoon climate and is the epicenter for many emerging infectious diseases. This study was conducted to better understand the prevalence and identity of the three pa ...
- DOI:
- 10.1186/s13071-019-3310-5
- https://dx.doi.org/10.1186/s13071-019-3310-5
- Author:
- Avendaño, Catalina; Ramo, Ana; Vergara-Castiblanco, Claudia; Sánchez-Acedo, Caridad; Quílez, Joaquín
- Source:
- Parasitology research 2018 v.117 no.5 pp. 1317-1323
- ISSN:
- 0932-0113
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium parvum; Protozoa; dairy calves; dairy farming; diarrhea; farms; feces; genes; glycoproteins; livestock and meat industry; microscopy; oocysts; probability; ribosomal RNA; sequence analysis; Colombia; Show all 17 Subjects
- Abstract:
- ... Fecal specimens from 432 pre-weaned calves younger than 35 days were collected over a 2-year period (2010–2012) from 74 dairy cattle farms in the central area of Colombia. These samples were microscopically examined for the presence of Cryptosporidium oocysts, and positive specimens were selected for molecular examination. Microscopy revealed that 115 calves (26.6%) from 44 farms (59.5%) tested po ...
- DOI:
- 10.1007/s00436-018-5818-6
- https://dx.doi.org/10.1007/s00436-018-5818-6
- Author:
- Santana, Bruna Nicoleti; Kurahara, Brayan; Nakamura, Alex Akira; da Silva Camargo, Vinícius; Ferrari, Elis Domingos; da Silva, Giane Serafim; Nagata, Walter Bertequini; Meireles, Marcelo Vasconcelos
- Source:
- Preventive veterinary medicine 2018 v.151 pp. 73-78
- ISSN:
- 0167-5877
- Subject:
- Cryptosporidium bovis, etc ; DNA; actin; chickens; feces; genes; genetic similarity; genotype; oocysts; polymerase chain reaction; poultry production; production technology; ribosomal RNA; sequence analysis; Brazil; Show all 15 Subjects
- Abstract:
- ... The objective of this study was to determine the occurrence of Cryptosporidium spp. in domestic chickens raised in different chicken production systems in Brazil using three nested PCR protocols. The purification and concentration of oocysts present in 190 fecal samples from chickens raised in extensive, semi-intensive and intensive production systems were accomplished by centrifugal flotation in ...
- DOI:
- 10.1016/j.prevetmed.2018.01.007
- https://dx.doi.org/10.1016/j.prevetmed.2018.01.007
- Author:
- Tao, Wei; Li, Yijing; Yang, Hang; Song, Mingxin; Lu, Yixin; Li, Wei
- Source:
- The Journal of parasitology 2018 v.104 no.1 pp. 10-17
- ISSN:
- 0022-3395
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium andersoni; Cryptosporidium parvum; Cryptosporidium ryanae; DNA; cities; cryptosporidiosis; dairy cattle; dominant species; genes; genetic traits; genotype; glycoproteins; livestock and meat industry; monitoring; parasites; polymerase chain reaction; public health; ribosomal RNA; sequence analysis; China; Show all 21 Subjects
- Abstract:
- ... Bovine cryptosporidiosis constitutes a threat to the livestock industry and public health worldwide. In the present study we investigated dairy cattle of all ages in northeast China for the prevalence and genetic traits of Cryptosporidium. Nested polymerase chain reaction of the small subunit rRNA gene was used to identify Cryptosporidium species or genotype. The parasite was detected in 130 of 53 ...
- DOI:
- 10.1645/17-140
- http://dx.doi.org/10.1645/17-140
- Author:
- Yap, Nan Jiun; Koehler, Anson V.; Ebner, Janine; Tan, Tiong Kai; Lim, Yvonne A.L.; Gasser, Robin B.
- Source:
- Molecular and cellular probes 2016 v.30 no.1 pp. 39-43
- ISSN:
- 0890-8508
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium ryanae; DNA; calves; cattle industry; cryptosporidiosis; farms; feces; polymerase chain reaction; public health; rural communities; zoonoses; Malaysia; Show all 13 Subjects
- Abstract:
- ... Despite the importance of the cattle industry in Malaysia, there are very few studies of the diversity and public health significance of bovine cryptosporidiosis in this country. In the present study, we used a PCR-based approach to detect and genetically characterize Cryptosporidium DNA in faecal samples from a cohort of 215 asymptomatic cattle (of different ages) from six farms from five states ...
- DOI:
- 10.1016/j.mcp.2016.01.002
- https://dx.doi.org/10.1016/j.mcp.2016.01.002
- Author:
- Zahedi, Alireza; Phasey, Jordan; Boland, Tony; Ryan, Una
- Source:
- Parasitology research 2016 v.115 no.3 pp. 1349-1353
- ISSN:
- 0932-0113
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium parvum; adults; buffaloes; epidemiological studies; feces; glycoproteins; humans; loci; oocysts; quantitative polymerase chain reaction; rivers; sequence analysis; waterways; Northern Territory; Show all 15 Subjects
- Abstract:
- ... A molecular epidemiological survey of Cryptosporidium from water buffalo (Bubalus bubalis) in the Northern Territory in Australia was conducted. Fecal samples were collected from adult farmed (n = 50) and wild buffalo (n = 50) and screened using an 18S quantitative PCR (qPCR). Positives were typed by sequence analysis of 18S nested PCR products. The qPCR prevalence of Cryptosporidium species in fa ...
- DOI:
- 10.1007/s00436-016-4901-0
- http://dx.doi.org/10.1007/s00436-016-4901-0
- Author:
- Mirhashemi, Marzieh Ezzaty; Zintl, Annetta; Grant, Tim; Lucy, Frances; Mulcahy, Grace; Waal, Theo De
- Source:
- Veterinary parasitology 2016 v.216 pp. 18-22
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium andersoni; Cryptosporidium parvum; Cryptosporidium ryanae; Protozoa; cattle; cryptosporidiosis; diarrhea; farms; genotype; horses; hosts; molecular epidemiology; sheep; spring; summer; swine; watersheds; Ireland; Show all 19 Subjects
- Abstract:
- ... Cryptosporidium is a protozoan that can cause gastro-intestinal illness with diarrhoea in a wide range of hosts. In fact some species of Cryptosporidium can infect the broad range of hosts. The current paper is focused to investigate monthly prevalence and diversity of Cryptosporidium spp. during the spring and early summer (March–June) in 2009 and 2010 in farms with no history of cryptosporidiosi ...
- DOI:
- 10.1016/j.vetpar.2015.12.002
- https://dx.doi.org/10.1016/j.vetpar.2015.12.002
- Author:
- Li, Pei; Cai, Jinzhong; Cai, Min; Wu, Wenxian; Li, Chunhua; Lei, Mengtong; Xu, Hailing; Feng, Lijun; Ma, Jiawen; Feng, Yaoyu; Xiao, Lihua
- Source:
- Veterinary parasitology 2016 v.215 pp. 58-62
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium andersoni; Cryptosporidium ryanae; buffaloes; cattle; cold; feces; free range husbandry; habitats; oxygen; polymerase chain reaction; sequence analysis; sheep; ultraviolet radiation; yaks; China; Show all 16 Subjects
- Abstract:
- ... Few data are available on the distribution of Cryptosporidium species in Tibetan sheep and yaks, which are free-range animals living in a cold, low oxygen, and high ultraviolet radiation habitat. In this study, 904 fecal specimens were collected from 350 Tibetan sheep and 554 yaks in six counties. Cryptosporidium spp. were detected and differentiated by PCR and sequence analyses. Altogether, 43 (1 ...
- DOI:
- 10.1016/j.vetpar.2015.11.009
- https://dx.doi.org/10.1016/j.vetpar.2015.11.009
- Author:
- BJÖRKMAN, C.; LINDSTRÖM, L.; OWESON, C.; AHOLA, H.; TROELL, K.; AXÉN, C.
- Source:
- Parasitology 2015 v.142 no.8 pp. 1108-1114
- ISSN:
- 1469-8161
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium parvum; Cryptosporidium ryanae; beef cattle; calves; calving; cleaning; clinical examination; cows; feces; genes; herds; information management; microscopy; oocysts; questionnaires; regression analysis; ribosomal RNA; Sweden; Show all 19 Subjects
- Abstract:
- ... A study was carried out to investigate how common Cryptosporidium infections are in beef calves in Swedish suckler herds and to explore which species and subtypes that occur. We further aimed at identifying factors associated with shedding of Cryptosporidium oocysts in this type of calf management. The study was conducted in two regions in Sweden and included 30 herds. Faecal samples were collecte ...
- DOI:
- 10.1017/S0031182015000426
- PubMed:
- 25899555
- PubMed Central:
- PMC4453919
- https://dx.doi.org/10.1017/S0031182015000426
- Author:
- Silverlås, C.; Bosaeus-Reineck, H.; Näslund, K.; Björkman, C.
- Source:
- International journal for parasitology 2013 v.43 pp. 155-161
- ISSN:
- 0020-7519
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium parvum; calves; color; cryptosporidiosis; diarrhea; feces; herds; microscopy; pathogens; Sweden; Show all 11 Subjects
- Abstract:
- ... Cryptosporidium parvum is a common pathogen in preweaned calves but in Sweden Cryptosporidium bovis, which is considered apathogenic, is the most common species in this age group and it has been identified in diarrhoeal samples, indicating that it could be a cause of diarrhoea. In routine diagnostic procedures, infection is determined by microscopy, which is not sufficient to differentiate these s ...
- DOI:
- 10.1016/j.ijpara.2012.10.009
- PubMed:
- 23142404
- http://dx.doi.org/10.1016/j.ijpara.2012.10.009
- Author:
- Smith, R.P.; Clifton-Hadley, F.A.; Cheney, T.; Giles, M.
- Source:
- Veterinary parasitology 2014 v.204 no.3-4 pp. 111-119
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium andersoni; Cryptosporidium parvum; adults; agricultural wastes; birds; calves; dairy cattle; dairy farming; farms; humans; indirect contact; microscopy; oocysts; pastures; rodents; sheep; slurries; wildlife; England; Wales; Show all 21 Subjects
- Abstract:
- ... An average of 70 samples were collected from 80 dairy farms in England and Wales, from cattle, co-grazed sheep, wildlife and farm wastes, to investigate prevalence, potential sources and transmission routes of Cryptosporidium. At least one positive sample was detected on 74 of the farms (92.5%) by IFAT microscopy. The prevalence in cattle was 10.2% (95% CI 9.4–11.1%), with greater prevalences dete ...
- DOI:
- 10.1016/j.vetpar.2014.05.022
- https://dx.doi.org/10.1016/j.vetpar.2014.05.022
16. Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle, northwest China
- Author:
- Zhang, Xiao-Xuan; Tan, Qi-Dong; Zhou, Dong-Hui; Ni, Xiao-Ting; Liu, Guang-Xue; Yang, Yan-Chuan; Zhu, Xing-Quan
- Source:
- Parasitology research 2015 v.114 no.7 pp. 2781-2787
- ISSN:
- 0932-0113
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium andersoni; Cryptosporidium parvum; Cryptosporidium ryanae; DNA; dairy cattle; digestive system diseases; feces; genes; humans; nucleotide sequences; polymerase chain reaction; ribosomal RNA; sequence analysis; species identification; China; Show all 16 Subjects
- Abstract:
- ... Cryptosporidium spp. cause enteric diseases in a wide range of animals, including dairy cattle. However, limited information is available regarding prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in Gansu province and Ningxia Hui Autonomous Region (NXHAR), northwest China. A total of 2945 dairy feces samples (1257 from Gansu province and 1688 from NXHAR) were coll ...
- DOI:
- 10.1007/s00436-015-4537-5
- http://dx.doi.org/10.1007/s00436-015-4537-5
- Author:
- Ma, Jingbo; Li, Pei; Zhao, Xiaoping; Xu, Hailing; Wu, Wenxian; Wang, Yuanfei; Guo, Yaqiong; Wang, Lin; Feng, Yaoyu; Xiao, Lihua
- Source:
- Veterinary parasitology 2015 v.207 no.3-4 pp. 220-227
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium andersoni; Cryptosporidium ryanae; Enterocytozoon; beef cattle; buffaloes; dairy cattle; diarrhea; farms; feces; genes; genotype; hosts; humans; internal transcribed spacers; mixed infection; polymerase chain reaction; protists; ribosomal RNA; sequence analysis; yaks; zoonoses; China; Show all 23 Subjects
- Abstract:
- ... Cryptosporidium spp. and Enterocytozoon bieneusi are important protists in a wide range of vertebrate hosts, causing diarrheal diseases. Cattle are considered potential reservoirs of Cryptosporidium infection in humans, although their role in the transmission of E. bieneusi is not clear. In the present work, 793 fecal specimens from dairy cattle, native beef cattle, and water buffaloes on 11 farms ...
- DOI:
- 10.1016/j.vetpar.2014.10.011
- https://dx.doi.org/10.1016/j.vetpar.2014.10.011
- Author:
- Abeywardena, Harshanie; Jex, Aaron R.; von Samson-Himmelstjerna, Georg; Haydon, Shane R.; Stevens, Melita A.; Gasser, Robin B.
- Source:
- Infection, genetics, and evolution 2013 v.20 pp. 96-102
- ISSN:
- 1567-1348
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium parvum; Giardia lamblia; buffaloes; farms; feces; genes; genotype; humans; mutation; pathogens; polymerase chain reaction; ribosomal RNA; surveys; triose-phosphate isomerase; Victoria (Australia); Show all 16 Subjects
- Abstract:
- ... We conducted a molecular epidemiological survey of Cryptosporidium and Giardia from Bubalus bubalis (water buffalo) on two extensive farms (450km apart) in Victoria, Australia. Faecal samples (n=476) were collected from different age groups of water buffalo at two time points (six months apart) and tested using a PCR-based mutation scanning-targeted sequencing-phylogenetic approach, employing mark ...
- DOI:
- 10.1016/j.meegid.2013.07.019
- PubMed:
- 23886616
- https://dx.doi.org/10.1016/j.meegid.2013.07.019
- Author:
- García-Presedo, Ignacio; Pedraza-Díaz, Susana; González-Warleta, Marta; Mezo, Mercedes; Gómez-Bautista, Mercedes; Ortega-Mora, Luis Miguel; Castro-Hermida, José Antonio
- Source:
- Veterinary parasitology 2013 v.197 no.3-4 pp. 658-664
- ISSN:
- 0304-4017
- Subject:
- Cryptosporidium bovis, etc ; Capreolus capreolus; Giardia lamblia; adults; enteropathogens; feces; fluorescence microscopy; genes; genotype; giardin protein; habitats; humans; oocysts; parasites; polymerase chain reaction; preserves; ribosomal RNA; Spain; Show all 18 Subjects
- Abstract:
- ... The aim of the present study was to identify the species and/or genotypes of Cryptosporidium and Giardia duodenalis infecting roe deer (Capreolus capreolus) in Galicia (NW Spain). The presence of both enteropathogens was investigated in 212 faecal samples from roe deer shot in diverse game preserves in three different areas of Galicia. The samples were analyzed by immunofluorescence microscopy and ...
- DOI:
- 10.1016/j.vetpar.2013.07.002
- PubMed:
- 23890824
- https://dx.doi.org/10.1016/j.vetpar.2013.07.002
- Author:
- Rieux, A.; Chartier, C.; Pors, I.; Delafosse, A.; Paraud, C.
- Source:
- Parasitology research 2013 v.112 no.10 pp. 3423-3431
- ISSN:
- 0932-0113
- Subject:
- Cryptosporidium bovis, etc ; Cryptosporidium parvum; alleles; dairy calves; excretion; farms; feces; genotyping; herds; loci; oocysts; polymerase chain reaction; ribosomal RNA; sequence analysis; France; Show all 15 Subjects
- Abstract:
- ... Ninety-two Cryptosporidium sp.-positive fecal samples of dairy diarrheic or non-diarrheic calves from 30 cattle herds in Normandy (France) were selected. Here, the aim was to investigate the species of Cryptosporidium excreted as well as the subtypes of Cryptosporidium parvum found in 7-17-day-old dairy calves. Excretion levels were comprised between 2 × 10(4) and 4 × 10(7) oocysts per gram of fec ...
- DOI:
- 10.1007/s00436-013-3520-2
- PubMed:
- 23820609
- http://dx.doi.org/10.1007/s00436-013-3520-2
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