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- Csörgő, B., et al. Show all 2 Author
- Acta biologica Hungarica 2007 v.58 no.0 pp. 1-10
- Escherichia coli; alleles; chromosome breakage; chromosomes; genetic engineering; homologous recombination; metabolites; molecular biology; operon; plasmids; proteins
- ... E. coli K-12 is the workhorse of molecular biology and the platform of choice for production of DNA, metabolites and proteins of industrial interest. To construct strains for the multitude of purposes, efficient genome manipulation methods are required. The suicide plasmid-mediated, homologous recombination-based gene replacement method is a convenient genome engineering tool. In consecutiv ...