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DNAbarcoding, etc ; Gloeophyllum trabeum; Picea abies; brown-rot fungi; decayed wood; forest litter; lignocellulose; woody biomass; Show all 8 Subjects
Abstract:
... In forest ecosystems, fungi are the key actors in wood decay. They have the capability to degrade lignified substrates and the woody biomass of coniferous forests, with brown rot fungi being common colonizers. Brown rots are typically involved in the earliest phase of lignocellulose breakdown, which therefore influences colonization by other microorganisms. However, few studies have focused on the ...
DNAbarcoding, etc ; biodiversity; community structure; data collection; decline; ecosystems; environment; environmental DNA; humans; land use; pollutants; riparian areas; rivers; Show all 13 Subjects
Abstract:
... Human-induced changes in land use drive an alarming decline in river biodiversity and related ecosystem services worldwide. However, how different land use shapes aquatic multitrophic communities is still not well understood. Here, we used the biodiversity dataset from bacteria to fish captured by the environmental DNA (eDNA) approach in the four riverine systems with spatially different land use ...
DNAbarcoding, etc ; barcoding; cost effectiveness; food safety; fraud; genes; genetic markers; human population; mitochondrial DNA; nanopores; nontarget organisms; seafoods; Show all 12 Subjects
Abstract:
... Seafood is the world's most highly traded food commodity. Due to the non-stop demand of the ever-increasing human population, the supply of certain species is not guaranteed, and mislabelling practices arise. Fillets and heavily processed products that cannot be easily authenticated are particularly vulnerable to fraudulent practices. In order to face this challenge, as a cost-effective, rapid, an ...
... Microplastic contamination poses a significant threat to agroecosystem functioning, provoking a move away from the use of conventional oil-based plastics in agriculture, to biodegradable alternatives that may be degraded over a shorter timescale. The impact of these bioplastics on plant and soil health, however, has received relatively little attention. Here, we investigated the effect of soil loa ...
Kristine Bohmann; Vasco Elbrecht; Christian Carøe; Iliana Bista; Florian Leese; Michael Bunce; Douglas W. Yu; Mathew Seymour; Alex J. Dumbrell; Simon Creer
... Metabarcoding of DNA extracted from environmental or bulk specimen samples is increasingly used to profile biota in basic and applied biodiversity research because of its targeted nature that allows sequencing of genetic markers from many samples in parallel. To achieve this, PCR amplification is carried out with primers designed to target a taxonomically informative marker within a taxonomic grou ...
DNAbarcoding, etc ; Lacertidae; mitochondrial DNA; phylogeny; taxonomy; Show all 5 Subjects
Abstract:
... The Kokshaal racerunner, Eremias kokshaaliensis Eremchenko et Panfilov, 1999, together with other central Asian racerunner species, is included in the Eremias multiocellata complex. In the present work, for the first time, the results of the analysis of historical mitochondrial DNA (barcode) are presented and the taxonomic status and preliminary phylogenetic relationships within the complex are sp ...
DNAbarcoding, etc ; indigenous species; phylogeny; taxonomy; Bulgaria; Show all 5 Subjects
Abstract:
... We present a study on the taxonomy of eleven Thymus species, belonging to two sections and occurring naturally in Bulgaria. Four DNA barcoding markers—matK, rbcL, trnH-psbA and ITS—were applied to discriminate the species and to reveal their phylogenetic relationships. The results showed that rbcL has the lowest discriminating power regarding the studied species, while the other markers yielded re ...
DNAbarcoding, etc ; DNA; chromatography; gold; nanocomposites; nanospheres; Show all 6 Subjects
Abstract:
... Self-assembly of DNA-labeled nanoparticles is an effective strategy to fabricate new nanocomposite materials and nanoscale devices from the bottom-up. To tailor the properties of the resulting material or device, one requires access to a wide range of nanoparticle sizes and shapes, as well as control over the number (valency) of DNA molecules on the nanoparticle surface. Currently, nanoparticles w ...
DNAbarcoding, etc ; Perlodidae; color; larvae; phylogeny; China; Show all 6 Subjects
Abstract:
... The larval morphology of Plecoptera is relatively insufficient in comparison with their adults. In this study, the larvae of Neofilchneria wanglanga Chen, 2019 and Skwala compacta (McLachlan 1872) from the family Perlodidae are described and illustrated based on specimens from China. The larvae of N. wanglanga and S. compacta share very similar body color patterns. Their morphological differences ...
DNAbarcoding, etc ; biodiversity; color; Antarctic region; Antarctica; Show all 5 Subjects
Abstract:
... The Southern Ocean is one of the most exposed regions to climate-related changes on our planet. Better understanding of the current biodiversity and past speciation events, as well as implementation of conservation actions and accurate identification of organisms to species level in this unique environment, is fundamental. In this study, two species of sea stars, Odontaster roseus Janosik & Halany ...
DNAbarcoding, etc ; forests; phylogeny; species richness; trees; China; Show all 6 Subjects
Abstract:
... Studying community assembly has always been a central issue in ecological research and is necessary for understanding mechanisms of species coexistence and biodiversity. Environmental heterogeneity is a driver of biodiversity, but much remains to be learned about how evolutionary processes are affected by environmental factors. We aimed to clarify the evolutionary processes in different vegetation ...
DNAbarcoding, etc ; Aeonium; codon usage; evolutionary adaptation; phylogeny; Show all 5 Subjects
Abstract:
... MAIN CONCLUSION: This study reported 13 new plastomes from Aeonium and Monanthes, and observed new markers for phylogeny and DNA barcoding, such as novel tRNA structures and codon usage bias and aversion. The Macaronesian clade of Crassulaceae consists of three genera: Aichryson, with about 15 species; Monanthes, with about 10 species; Aeonium, with about 40 species. Within this clade, Aeonium, kn ...
DNAbarcoding, etc ; biodiversity; fauna; haplotypes; mitochondria; morphs; taxonomy; Show all 7 Subjects
Abstract:
... Deep-sea corals are important benthic inhabitants that support the biodiversity and function of the wider faunal community; however, their taxonomy is underdeveloped and their accurate identification is often difficult. In our study, we investigated the utility of a superextended (>3000 bp) barcode and explored the effectiveness of various molecular species delimitation techniques with an aim to p ...
DNAbarcoding, etc ; fish; genes; oligodeoxyribonucleotides; species diversity; zooplankton; Show all 6 Subjects
Abstract:
... Metabarcoding to determine the species composition and diversity of marine zooplankton communities is a fast‐developing field in which the standardization of methods is yet to be fully achieved. The selection of genetic markers and primer choice are particularly important because they substantially influence species detection rates and accuracy. Validation is therefore an important step in the des ...
DNAbarcoding, etc ; Naegleria; new species; phylogeny; protists; soil; Show all 6 Subjects
Abstract:
... Heterolobosea is one of the major protist groups in soils. While an increasing number of soil heterolobosean species has been described, we have likely only scratched the surface of heterolobosean diversity in soils. Here, we expand this knowledge by morphologically and molecularly classifying four novel strains. One was identified as Naegleria clarki, while the remaining three strains had no iden ...
DNAbarcoding, etc ; community ecology; genetic testing; rapid methods; Show all 4 Subjects
Abstract:
... The community of biologists has been eager to realize the promise of DNA barcodes since the concept of a rapid method for genetic identification of species was first proposed in 2003 [...] ...
DNAbarcoding, etc ; Rhododendron; biodiversity; plastid genome; ribosomal DNA; Show all 5 Subjects
Abstract:
... Standard plant DNA barcodes based on 2–3 plastid regions, and nrDNA ITS show variable levels of resolution, and fail to discriminate among species in many plant groups. Genome skimming to recover complete plastid genome sequences and nrDNA arrays has been proposed as a solution to address these resolution limitations. However, few studies have empirically tested what gains are achieved in practice ...
DNAbarcoding, etc ; Halyomorpha halys; citizen science; haplotypes; Germany; Show all 5 Subjects
Abstract:
... AbstractThe invasive pentatomid true bug Halyomorpha halys is reported for the first time from the German state of Hamburg, along with records from several other locations in Northern Germany. Specimens collected by scientists were evaluated along with records from citizen science platforms. DNA barcode sequences of several H. halys specimens from Hamburg were evaluated along with 42 sequences of ...
DNAbarcoding, etc ; Achillea millefolium; genes; mesophyll; rhizomes; secondary metabolites; Show all 6 Subjects
Abstract:
... Ilham M,Mukarromah SR, Rakashiwi GA, Indriati DT, Yoku BF, Purnama PR, Junairiah, Prasongsuk S,Purnobasuki H, Wahyuni DK. 2022. Morpho-anatomical characterizationand DNA barcoding of Achillea millefolium L. Biodiversitas 23: 1958-1969. One of the important things to study the distribution of secondary metabolites in the plant body is to carry out the identification process. Morphological markers h ...
DNAbarcoding, etc ; community structure; environmental DNA; fish; rivers; streams; Show all 6 Subjects
Abstract:
... Environmental DNA (eDNA) techniques can be used to help monitor the presence of aquatic organisms without causing a disturbance and are expected to become useful tools in aquatic environmental monitoring. The results of eDNA techniques are largely affected by the sampling method used. Few studies have investigated the effects of diurnal and seasonal changes on the detection of fish species using e ...