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1. Application of SRAP Markers for DNA Identification of Russian Alfalfa Cultivars

Author:
Shamustakimova, A. O.; Mavlyutov, Y. M.; Klimenko, I. A.
Source:
Russian journal of genetics 2021 v.57 no.5 pp. 540-547
ISSN:
1022-7954
Subject:
DNA fingerprinting,   etc   ; DNA; alfalfa; certification; cultivars; genetic variation;     Show all 6 Subjects
Abstract:
... This study was carried out to estimate the genetic diversity between cultivars of alfalfa using SRAP marker system (sequence-related amplified polymorphism). We tested 25 combinations of SRAP markers and revealed that seven of them were informative for DNA polymorphism identification. These combinations generated 129 PCR products with the percentage of polymorphic bands per pair ranging from 21 to ...
DOI:
10.1134/S1022795421050112

2. An 18 Multi‐InDels panel for analysis of highly degraded forensic biological samples

Author:
Li, Jienan; Lin, Lin; Jiang, Bowei; Wang, Chudong; Zeye, Moutanou Modeste Judes; Wen, Dan; He, Wei; Qu, Weifeng; Liu, Ying; Zha, Lagabaiyila
Source:
Electrophoresis 2021 v.42 no.9-10 pp. 1143-1152
ISSN:
0173-0835
Subject:
DNA fingerprinting,   etc   ; DNA; capillary electrophoresis; forensic sciences; genotyping; humans; parentage; probability; China;     Show all 9 Subjects
Abstract:
... DNA genotyping from trace and highly degraded biological samples is one of the most significant challenges of forensic DNA identification. There is a lack of simple and effective methods for genotyping highly degraded samples. In this study, a multiple loci insertion/deletion polymorphisms (Multi‐InDels) panel was designed for detecting 18 autosomal Multi‐InDels through capillary electrophoresis ( ...
DOI:
10.1002/elps.202000245

3. Identification and characterization of white onion (Allium cepa L.) genotypes for high total soluble solid content through molecular markers

Author:
Singh, Puthem Robindro; Mahajan, Vijay; Verma, Annu; Sagar, R.; Jayaswall, Kuldip; Shukla, Neeraj; Gedam, Pranjali; Singh, Major
Source:
Genetic resources and crop evolution 2021 v.68 no.3 pp. 957-967
ISSN:
0925-9864
Subject:
DNA fingerprinting,   etc   ; Allium cepa; DNA; bulbs; evolution; heterozygosity; introns; loci; onions; total soluble solids;     Show all 10 Subjects
Abstract:
... Onion (Allium cepa L.) is one of the most important bulbous vegetable used in global kitchen. Although it has various important traits but among them the high total soluble solid content (HTSS) of white onion is highly preferred by the industries for processing purpose. Looking into the broader use of the white onion; we utilized a total of 23 polymorphic simple sequence repeats (SSRs) and intron ...
DOI:
10.1007/s10722-020-01037-9

4. Intraspecific trait variability and genetic diversity in the adaptive strategies of serpentine and non-serpentine populations of Silene paradoxa L.

Author:
Lazzaro, Lorenzo; Colzi, Ilaria; Ciampi, Daniele; Gonnelli, Cristina; Lastrucci, Lorenzo; Bazihizina, Nadia; Viciani, Daniele; Coppi, Andrea
Source:
Plant and soil 2021 v.460 no.1-2 pp. 105-121
ISSN:
0032-079X
Subject:
DNA fingerprinting,   etc   ; Silene; gene pool; genetic drift; genetic variation; genome; leaves; serpentine; soil types;     Show all 9 Subjects
Abstract:
... AIMS: We investigated whether individuals of Silene paradoxa L., grown in serpentine and non-serpentine soils, displayed variation in functional traits and adaptive strategies together with a differentiation of the gene pool. We hypothesised that individuals growing in serpentine sites may be exposed to a higher degree of stress, resulting in measurable differences in leaf traits and adaptive stra ...
DOI:
10.1007/s11104-020-04780-1

5. Biochemical and molecular changes of the zosteric acid-treated Escherichia coli biofilm on a mineral surface

Author:
Villa, Federica; Secundo, Francesco; Forlani, Fabio; Cattò, Cristina; Cappitelli, Francesca
Source:
Annals of microbiology 2021 v.71 no.1 pp. 3
ISSN:
1590-4261
Subject:
DNA fingerprinting,   etc   ; DNA replication; Escherichia coli; adhesion; biofilm; biomass; carbohydrates; cells; flow; kinetics; microbiology; proteins; proteome; selenides; stress response; zinc;     Show all 16 Subjects
Abstract:
... PURPOSE: The main goal of the present work was to assess the effectiveness of zosteric acid (ZA) in hindering Escherichia coli biofilm formation on a mineral surface. METHODS: Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) flow system was used to probe in situ the biochemical changes induced by ZA on E. coli sessile cells growing on the zinc selenide ATR plate. Comparative prot ...
DOI:
10.1186/s13213-020-01617-1

6. Assessment of fish diversity in the South China Sea using DNA taxonomy

Author:
Xu, Lei; Wang, Xuehui; Van Damme, Kay; Huang, Delian; Li, Yafang; Wang, Lianggen; Ning, Jiajia; Du, Feiyan
Source:
Fisheries research 2021 v.233 pp. 105771
ISSN:
0165-7836
Subject:
DNA fingerprinting,   etc   ; ; DNA; DNA barcoding; fisheries; genes; marine fish; molecular systematics; research; Pacific Ocean; South China Sea;     Show all 11 Subjects
Abstract:
... The South China Sea (SCS) is part of the Indo-West Pacific Ocean, which is regarded as harboring a high diversity of fish. However, our knowledge of true fish diversity especially among pelagic and deep-sea families is still insufficient. Here, we used DNA barcoding and DNA identification to investigate the fish diversity and distribution in the three most important fisheries regions in the South ...
DOI:
10.1016/j.fishres.2020.105771

7. Body fluid identification: A case for more research and innovation

Author:
Williams, Graham A
Source:
Forensic science international 2020 v.2 pp. 100096
ISSN:
2665-9107
Subject:
DNA fingerprinting,   etc   ; body fluids; crime; fluids; forensic sciences; research; testing;     Show all 7 Subjects
Abstract:
... Body fluids are valuable evidence in a range of offences, with tests regularly being used to identify them. However, with the + increasing sensitivity of DNA profiling, capability gaps are becoming increasingly obvious. DNA profiles can also be obtained from body fluids which cannot be identified. Efforts to improve the capability of BFID have focused on genetic strategies. However, such technique ...
DOI:
10.1016/j.fsir.2020.100096

8. Territorial Subdivision of the Megalopolis Population by the Ethnic Trait in Relation to the Problem of Creating Genetic Databases: Moscow

Author:
Gracheva, A. S.; Pobedonostseva, E. Yu.; Udina, I. G.; Kurbatova, O. L.
Source:
Russian journal of genetics 2020 v.56 no.12 pp. 1520-1529
ISSN:
1022-7954
Subject:
DNA fingerprinting,   etc   ; multicultural diversity; population structure; topography; urban areas;     Show all 5 Subjects
Abstract:
... On the basis of the materials of the 2010 All-Russian Population Census in Moscow, an analysis of the unevenness of the settlement of ethnic groups in the urban area is carried out. Maps reflecting the “ethnic topography” of the megalopolis have been constructed. Using the attribute “nationality” as a “quasi-genetic” marker, estimates of the level of territorial subdivision of the megalopolis popu ...
DOI:
10.1134/S1022795420120078

9. An international survey into the analysis and interpretation of microscopic hair evidence by forensic hair examiners

Author:
Wilkinson, Laura; Gwinnett, Claire
Source:
Forensic science international 2020 v.308 pp. 110158
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; crime; forensic sciences; interviews; microscopy; police; surveys;     Show all 7 Subjects
Abstract:
... Due to inadequacies in historical hair examinations, the use and perceived value of microscopic hair evidence has reduced. Many reports have heavily criticised the use of pattern-based evidence including hair evidence as being unreliable, with police forces focussing on more individualising evidence. Hair evidence has been utilised in casework for many years due to its ability to transfer easily b ...
DOI:
10.1016/j.forsciint.2020.110158

10. Prevalence of DNA from the driver, passengers and others within a car of an exclusive driver

Author:
Boyko, Toni; Szkuta, B.; Mitchell, R. John; van Oorschot, Roland A.H.
Source:
Forensic science international 2020 v.307 pp. 110139
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; DNA; forensic sciences; sampling; vehicles (equipment);     Show all 5 Subjects
Abstract:
... Cars are often sampled for DNA to help identify occupants and their possible location(s) within the car. While DNA from the frequent driver is likely to accumulate over time, DNA from previous and/or subsequent occupants, and those whose DNA has inadvertently been transferred to the car, may also contribute to any samples collected. This study investigates how much DNA resides on various sites wit ...
DOI:
10.1016/j.forsciint.2020.110139

11. Nanoconfinement-Induced DNA Reptating Motion and Analogy to Fluctuating Interfaces

Author:
Yeh, Jia-Wei; Taloni, Alessandro; Sriram, K. K.; Shen, Jie-Pan; Kao, Der-You; Chou, Chia-Fu
Source:
Macromolecules 2020 v.53 no.3 pp. 1001-1013
ISSN:
1520-5835
Subject:
DNA fingerprinting,   etc   ; DNA; geometry; polymers; quantitative analysis; roughness;     Show all 6 Subjects
Abstract:
... Macromolecules in an entangled environment are constrained to wriggle predominantly along a confining tube, giving rise to the so-called reptation or tube-like motion. While the principles of polymer physics were well developed to understand its conformational dynamics, the quantitative characterization of the tube diameter and resulting reptation remains an open question. Here, using highly confi ...
DOI:
10.1021/acs.macromol.9b02074

12. A new digital approach to SNP encoding for DNA identification

Author:
Garafutdinov, Ravil R.; Sakhabutdinova, Assol R.; Slominsky, Petr A.; Aminev, Farit G.; Chemeris, Alexey V.
Source:
Forensic science international 2020 v.317 pp. 110520
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; biometry; computer software; forensic sciences; genetic testing; humans; nucleotides;     Show all 7 Subjects
Abstract:
... Identification of individuals has become an urgent problem for mankind. In the last three decades, STR-based DNA identification has actively evolved along with traditional biometric methods. Nonetheless, single-nucleotide polymorphisms (SNPs) are now of great interest and a number of relevant SNP panels have been proposed for DNA identification. Here, a simple approach to SNP data digitization tha ...
DOI:
10.1016/j.forsciint.2020.110520

13. A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans

Author:
Jung, Kwang-Woo; Lee, Kyung-Tae; Bahn, Yong-Sun
Source:
The journal of microbiology 2020 v.58 no.10 pp. 823-831
ISSN:
1225-8873
Subject:
DNA fingerprinting,   etc   ; Cryptococcus neoformans; antifungal agents; drug resistance; microbiology; mutagenesis; mutants; nucleotide sequences; pathogens; phenotype; virulence;     Show all 11 Subjects
Abstract:
... Signature-tagged mutagenesis (STM) is a high-throughput genetic technique that can be used to investigate the function of genes by constructing a large number of mutant strains with unique DNA identification tags, pooling them, and screening them for a particular phenotypic trait. STM was first designed for the identification of genes that contribute to the virulence or infectivity of a pathogen i ...
DOI:
10.1007/s12275-020-0341-8

14. Y Chromosome as a Tool for DNA Identification and Determination of Ethnoterritorial Origin

Author:
Kharkov, V. N.; Zarubin, A. A.; Vagaitseva, K. V.; Radzhabov, M. O.; Novikova, L. M.; Valikhova, L. V.; Khitrinskaya, I. Y.; Stepanov, V. A.
Source:
Russian journal of genetics 2020 v.56 no.9 pp. 1109-1117
ISSN:
1022-7954
Subject:
DNA fingerprinting,   etc   ; Y chromosome; dominance (genetics); genes; genetic variation; genotyping; haplotypes;     Show all 7 Subjects
Abstract:
... Genetic diversity of a large number of populations is analyzed using various Y-chromosome markers. Genotyping of a wide range of novel highly informative SNP and YSTR markers shows that most of the Y-chromosome haplogroups can be divided not only into ethnically specific lineages but also into narrower sublineages and clusters of haplotypes. A significant extent of population and interethnic genet ...
DOI:
10.1134/S1022795420090112

15. Comparative Analysis of ANDE 6C Rapid DNA Analysis System and Traditional Methods

Author:
Ragazzo, Michele; Melchiorri, Stefano; Manzo, Laura; Errichiello, Valeria; Puleri, Giulio; Nicastro, Fabio; Giardina, Emiliano
Source:
Genes 2020 v.11 no.5
ISSN:
2073-4425
Subject:
DNA fingerprinting,   etc   ; DNA; DNA fragmentation; alleles; automation; capillary electrophoresis; forensic sciences; nuclear genome; polymerase chain reaction;     Show all 9 Subjects
Abstract:
... Rapid DNA analysis is an ultrafast and fully automated DNA-typing system, which can produce interpretable genetic profiles from biological samples within 90 minutes. This “swab in—profile out” method comprises DNA extraction, amplification by PCR multiplex, separation and detection of DNA fragments by capillary electrophoresis. The aim of study was the validation of the Accelerated Nuclear DNA Equ ...
DOI:
10.3390/genes11050582

16. Revealing new leads for the impact of galacto-oligosaccharides on gut commensals and gut health benefits through text mining

Author:
Sijbers, A.M.; Schoemaker, R.J.W.; Nauta, A.; Alkema, W.
Source:
Beneficial microbes 2020 v.11 no.3 pp. 283-302
ISSN:
1876-2891
Subject:
DNA fingerprinting,   etc   ; Bifidobacterium; constipation; databases; digestive system; ecosystems; epithelium; galactooligosaccharides; humans; metagenomics; ribosomal DNA;     Show all 11 Subjects
Abstract:
... Galacto-oligosaccharides (GOS) are linked to various health benefits, such as the relief of symptoms of constipation. Part of the beneficial effects of GOS are thought to be the consequence of their bifidogenic effect, stimulating the growth of several Bifidobacterium species in vivo. However, GOS may exert additional effects by directly stimulating other bacterial species or by effects that bifid ...
DOI:
10.3920/BM2019.0105

17. Novel 28 microsatellite loci using high-throughput sequencing for an endangered species on Metasequoia glyptostroboides (Cupressaceae)

Author:
Wang, Jing-Wen; Xu, Tong-Lei; Sereke, Ghenet Weldegebriel; Wang, Rong; Li, Yuan-Yuan
Source:
Molecular biology reports 2020 v.47 no.4 pp. 2991-2996
ISSN:
0301-4851
Subject:
DNA fingerprinting,   etc   ; Metasequoia glyptostroboides; endangered species; germplasm; loci; microsatellite repeats; molecular biology;     Show all 7 Subjects
Abstract:
... Metasequoia glyptostroboides is a living fossil and an endangered species listed in the International Union for Conservation of Nature (IUCN). Distinguishing the genotypes of all wild individuals of M. glyptostroboides is important to delimit management units and key germplasm resources. We characterized 28 novel polymorphic microsatellite loci using a streptavidin–biotin microsatellite-enriched l ...
DOI:
10.1007/s11033-020-05303-y

18. Species delimitation within Campomanesia (Myrtaceae) using morphology and amplification profiles of microsatellite markers

Author:
Luber, Jaquelini; Christ, Jheniffer Abeldt; Ferreira, Marcia Flores da Silva; Carrijo, Tatiana Tavares
Source:
Revista brasileira de botânica 2020 v.43 no.1 pp. 131-137
ISSN:
0100-8404
Subject:
DNA fingerprinting,   etc   ; Campomanesia; cluster analysis; genetic markers; heat; microsatellite repeats; plant identification; regression analysis; species identification;     Show all 9 Subjects
Abstract:
... Campomanesia is a well-circumscribed genus within Myrtaceae, but the boundaries among some of its species remain poorly understood. Microsatellite amplification profiles have proven useful DNA fingerprinting for plant species identification, but have never been applied to the taxonomy of this genus. Here we studied 12 species of Campomanesia, including a set of related and non-related species, as ...
DOI:
10.1007/s40415-020-00583-x

19. Development of a core SNP panel for cacao (Theobroma cacao L.) identity analysis

Author:
Mahabir, Amrita; Motilal, Lambert A.; Gopaulchan, David; Ramkissoon, Saila; Sankar, Antoinette; Umaharan, Pathmanathan
Source:
Genome 2020 v.63 no.2 pp. 103-114
ISSN:
1480-3321
Subject:
DNA fingerprinting,   etc   ; Theobroma cacao; data collection; gene frequency; heterozygosity; linkage disequilibrium; linkage groups; single nucleotide polymorphism;     Show all 8 Subjects
Abstract:
... Single nucleotide polymorphisms (SNPs) are preferred markers for DNA fingerprinting and diversity studies in cacao (Theobroma cacao L.). Yet, a consensus SNP panel with a minimum number of SNPs for optimal identity analysis is unavailable for cacao. An initial set of 146 SNP panels of varying sizes were assembled based on heterozygosity, linkage disequilibrium (LD), linkage group (LG) distribution ...
DOI:
10.1139/gen-2019-0071

20. Genetic analyses reveal complex introduction histories for the invasive tree Acacia dealbata Link around the world

Author:
Hirsch, Heidi; Richardson, David M.; Pauchard, Aníbal; Le Roux, Johannes J.
Source:
Diversity & distributions 2021 v.27 no.2 pp. 360-376
ISSN:
1366-9516
Subject:
DNA fingerprinting,   etc   ; Acacia dealbata; Bayesian theory; administrative management; data collection; exhibitions; genetic analysis; genetic markers; genetic structure; genetic variation; history; invasive species; knowledge; population; research; sampling; testing; Chile; Madagascar; New Zealand; Portugal; Reunion; South Africa; Tasmania; United States;     Show all 25 Subjects
Abstract:
... AIM: To compare genetic diversity and structure between Acacia dealbata populations sampled across the species’ native range in Australia and from its non‐native ranges in Chile, Madagascar, New Zealand, Portugal, La Réunion island, South Africa and the United States, and to investigate the most likely introduction scenarios to non‐native ranges. LOCATION: Global. TAXON: Acacia dealbata, Fabaceae. ...
DOI:
10.1111/ddi.13186