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1. DNA recovery from gelatin fingerprint lifters by direct proteolytic digestion

Author:
Zieger, Martin; Schneider, Christoph; Utz, Silvia
Source:
Forensic science international 2019 v.295 pp. 145-149
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; DNA; forensic sciences; gelatin; magnetism; proteolysis; protocols; staining;     Show all 8 Subjects
Abstract:
... Fingerprints are a valuable source for DNA profiling in forensic investigations. In practice, the fingerprints are routinely visualized first by powder staining and then often transferred to tapes or gelatin lifters for storage or examination. If at all, fingerprints are usually sampled for DNA in a second step. To target the DNA sampling in an optimal way, it is essential to know how much of the ...
DOI:
10.1016/j.forsciint.2018.12.006

2. Advances in the systematics and evolution of western Mediterranean representative species of the Pentanema conyzae clade through genetic fingerprinting

Author:
Gutiérrez‐Larruscain, David; Andrés‐Sánchez, Santiago; Rico, Enrique; Martínez‐Ortega, María Montserrat
Source:
Journal of systematics and evolution 2019 v.57 no.1 pp. 42-54
ISSN:
1674-4918
Subject:
DNA fingerprinting,   etc   ; amplified fragment length polymorphism; evolution; Iberian Peninsula;     Show all 4 Subjects
Abstract:
... Forty‐five populations of Pentanema corresponding to seven species included in the Pentanema conyzae clade have been studied using AFLP fingerprinting. The results show that allopolyploidization could have been involved in the diversification of this group, specifically in species P. langeanum and P. maletii. Molecular data confirm the presence of P. britannicum in the Iberian Peninsula and key st ...
DOI:
10.1111/jse.12431

3. Time since intercourse (TSI) data from a large-scale casework study of penile–vaginal penetration allegations using Sperm Elution​™

Author:
Owers, R.; Davidson, G.; McDonald, A.; Morgan, R.; O’Rourke, P.
Source:
Forensic science international 2018 v.288 pp. 10-13
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; forensic sciences; males; spermatozoa;     Show all 4 Subjects
Abstract:
... Time since intercourse (TSI) expectations are dependent on the method used to recover spermatozoa from vaginal swabs. TSI data following Sperm Elution™ is presented from a large scale study of 2269 cases of penile–vaginal penetration sexual assault allegations analysed by Cellmark Forensic Services and is compared to published TSI data generated using two different water-based elution methods Sper ...
DOI:
10.1016/j.forsciint.2018.04.014

4. Direct STR typing from fired and unfired bullet casings

Author:
Thanakiatkrai, Phuvadol; Rerkamnuaychoke, Budsaba
Source:
Forensic science international 2019 v.301 pp. 182-189
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; DNA; alleles; forensic sciences; microsatellite repeats; models; polymerase chain reaction;     Show all 7 Subjects
Abstract:
... Cartridges, bullets, and casings (CBCs) are commonly recovered after shooting incidents and could provide valuable DNA information from touch DNA that has been left behind during handling of bullets and loading of guns. Direct PCR, in which the DNA extraction and quantification steps are bypassed, has been shown to provide comparable and sometimes better results from touch DNA and trace DNA sample ...
DOI:
10.1016/j.forsciint.2019.05.037

5. A practical study on direct PCR amplification using the GlobalFiler™ PCR Amplification Kit on human bloodstains collected with microFLOQ™ Direct swabs

Author:
Chong, Kevin Wai Yin; Wong, Yongxun; Ng, Boon Kiat; Lim, Wei Siong Holden; Rosli, Afiqah Razanah; Syn, Christopher Kiu Choong
Source:
Forensic science international 2019 v.300 pp. 43-50
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; DNA; blood; capillary electrophoresis; denim; humans; law enforcement; leather; nylon; paper; polymerase chain reaction;     Show all 11 Subjects
Abstract:
... Rapid DNA profiling of casework samples is a powerful tool that can support law enforcement agencies in the quick apprehension of perpetrators before they re-offend or escape the jurisdiction. This present study evaluated the feasibility of direct PCR amplification, using the microFLOQ™ Direct swab, for generating DNA profiles (from bloodstains) within 3 h. The swab tip is coated with nylon fibers ...
DOI:
10.1016/j.forsciint.2019.04.018

6. DNA kinship analysis of unidentified human remains that led to a murder investigation

Author:
Starinsky-Elbaz, Sigal; Ariel, Tal; Issan, Yossi
Source:
Forensic science international 2019 v.300 pp. e20
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; DNA; databases; forensic sciences; humans; kinship; metadata; pedigree; people; Israel;     Show all 10 Subjects
Abstract:
... DNA-based analysis is an important tool for the identification of unidentified human remains (UHR). Comparing DNA of UHR with DNA profiles of missing persons’ relatives may provide genetic data leading to identification. In most cases, DNA profiles of missing persons are not available and therefore, the identification process must be based on kinship analysis.UHR were found buried in Southern Isra ...
DOI:
10.1016/j.forsciint.2019.03.045

7. Enhancement of fingermarks and visualizing DNA

Author:
Kanokwongnuwut, P.; Kirkbride, K.P.; Kobus, H.; Linacre, A.
Source:
Forensic science international 2019 v.300 pp. 99-105
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; DNA; aluminum; dyes; fluorescence; forensic sciences; magnetism; powders; staining; vapors;     Show all 10 Subjects
Abstract:
... A novel method for detection and visualization of latent DNA using Diamond™ Nucleic Acid Dye (DD) staining has been developed. Applying DD to an object has the real potential to visualize DNA on a substrate from which a DNA profile can be generated, but it is important to determine whether this staining will adversely affect other forensic investigational techniques and vice versa.The aim of this ...
DOI:
10.1016/j.forsciint.2019.04.035

8. Using the information embedded in the mixed profiles to assist in determining the identity of the deceased and the suspect in a deficiency case

Author:
Xiao, Chao; Jiang, Yanwei; Liang, Man
Source:
Forensic science international 2019 v.300 pp. e13
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; DNA; blood sampling; databases; females; genotyping; loci; males; probability; women; worker honey bees;     Show all 11 Subjects
Abstract:
... In the present case, due to the lack of database matches and available relatives, the use of single-source DNA profiles from the unidentified deceased and the suspect was not yet able to determine the identity of them, resulting in the inability to continue the investigation. However, by interpreting a simple mixture on the penile swab of the deceased and a complex mixture on the prayer flag wrapp ...
DOI:
10.1016/j.forsciint.2019.04.022

9. Poverty Reduction Effects of Agricultural Technology Adoption: The Case of Improved Cassava Varieties in Nigeria

Author:
Wossen, Tesfamicheal; Alene, Arega; Abdoulaye, Tahirou; Feleke, Shiferaw; Rabbi, Ismail Y.; Manyong, Victor
Source:
Journal of agricultural economics 2019 v.70 no.2 pp. 392-407
ISSN:
0021-857X
Subject:
DNA fingerprinting,   etc   ; cassava; farmers; household income; income distribution; innovation adoption; models; poverty; Nigeria;     Show all 9 Subjects
Abstract:
... We use DNA‐fingerprinting to estimate the poverty reduction effect of adoption of improved cassava varieties in Nigeria. We estimate the counterfactual household income distribution of cassava producers by combining farm‐level treatment effects with a market‐level model. Our results suggest that adoption of improved cassava varieties has led to a 4.6 percentage point reduction in poverty, though t ...
DOI:
10.1111/1477-9552.12296

10. Development and validation of the RapidHIT™ 200 utilising NGMSElect™ Express for the processing of buccal swabs

Author:
Shackleton, David; Pagram, Jenny; Ives, Lesley; Vanhinsbergh, Des
Source:
Forensic science international 2018 v.289 pp. 244-252
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; DNA; databases; polymerase chain reaction; United Kingdom;     Show all 5 Subjects
Abstract:
... The RapidHIT™ 200 System is a fully automated sample-to-DNA profile system designed to produce high quality DNA profiles within 2h. The use of RapidHIT™ 200 System within the United Kingdom Criminal Justice System (UKCJS) has required extensive development and validation of methods with a focus on AmpFℓSTR® NGMSElect™ Express PCR kit to comply with specific regulations for loading to the UK Nation ...
DOI:
10.1016/j.forsciint.2018.05.041

11. Impact of one-step luminescent cyanoacrylate treatment on subsequent DNA analysis

Author:
Khuu, Alicia; Chadwick, Scott; Moret, Sébastien; Spindler, Xanthe; Gunn, Peter; Roux, Claude
Source:
Forensic science international 2018 v.286 pp. 1-7
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; DNA; alleles; crime; forensic sciences; luminescence; saliva;     Show all 7 Subjects
Abstract:
... Fingermarks can be exploited for both their ridge detail and touch DNA. One-step luminescent cyanoacrylate (CA) fuming techniques used for fingermark enhancement, such as PolyCyano UV (Foster+Freeman Ltd) and Lumicyano™ (Crime Science Technology), claim to be compatible with DNA analysis as they reduce the need for post‐staining to increase contrast of the developed fingermark. The aim of this stu ...
DOI:
10.1016/j.forsciint.2018.02.015

12. Regional variations in human papillomavirus prevalence across time in NHANES (2003–2014)

Author:
Hirth, Jacqueline M.; Kuo, Yong-Fang; Starkey, Jonathan M.; Rupp, Richard E.; Laz, Tabassum H.; Rahman, Mahbubur; Berenson, Abbey B.
Source:
Vaccine 2019 v.37 no.30 pp. 4040-4046
ISSN:
0264-410X
Subject:
DNA fingerprinting,   etc   ; National Health and Nutrition Examination Survey; Papillomaviridae; females; models; regression analysis; risk; uterine cervical neoplasms; vaccination; vaccines;     Show all 10 Subjects
Abstract:
... The consequences of low human papillomavirus (HPV) vaccination in Census regions with higher incidence of cervical cancer may contribute to continued disparities. Our purpose was to evaluate regional variations in HPV prevalence across time.Repeated cross-sectional data from the National Health and Nutrition Examination Survey (NHANES), 2003–2014 were examined. Participants included females 14 to ...
DOI:
10.1016/j.vaccine.2019.06.001

13. AFLP protocol comparison for microbial diversity fingerprinting

Author:
Bertani, Gaia; Savo Sardaro, Maria Luisa; Neviani, Erasmo; Lazzi, Camilla
Source:
Journal of applied genetics 2019 v.60 no.2 pp. 217-223
ISSN:
1234-1983
Subject:
DNA fingerprinting,   etc   ; DNA; Lactobacillus plantarum; Listeria monocytogenes; Streptococcus thermophilus; amplified fragment length polymorphism; bacteria; biodiversity; genomics; genotyping; taxonomy;     Show all 11 Subjects
Abstract:
... Over the last decade, several methods based on genomic DNA have been developed for the identification and genotyping of prokaryotic and eukaryotic organisms. These genomic methods differ regarding taxonomic range, discriminatory power, reproducibility, and ease of interpretation and standardization. The amplified fragment length polymorphism (AFLP) technique is a very powerful DNA fingerprinting t ...
DOI:
10.1007/s13353-019-00492-0

14. Isolation and characterization of spontaneous phage-resistant mutants of Lactobacillus paracasei

Author:
Sunthornthummas, Sirinthorn; Doi, Katsumi; Rangsiruji, Achariya; Krajangsung, Sukhumaporn; Sarawaneeyaruk, Siriruk; Pringsulaka, Onanong
Source:
Food control 2019 v.99 pp. 114-123
ISSN:
0956-7135
Subject:
DNA fingerprinting,   etc   ; Lactobacillus paracasei; acidification; agar; bacteriophages; cold storage; fermentation; milk; mutants; random amplified polymorphic DNA technique;     Show all 10 Subjects
Abstract:
... Spontaneous phage-resistant mutants were isolated from Lactobacillus paracasei LPC by agar plate (AP) and secondary-culture (SC) methods. They were characterized by cell and colony morphologies, carbohydrate fermentation patterns, phage resistance stability, efficiency of plaquing (EOP), acidifying and milk acidification kinetics. Only 98 out of 175 isolates (56%) proved to be true phage-resistant ...
DOI:
10.1016/j.foodcont.2018.12.037

15. The impact of burial period on compact bone microstructure: Histological analysis of matrix loss and cell integrity in human bones exhumed from tropical soil

Author:
Astolphi, Rafael Dias; de Seixas Alves, Maria Teresa; Evison, Martin Paul; Francisco, Raffaela Arrabaça; Guimarães, Marco Aurelio; Iwamura, Edna Sadayo Miazato
Source:
Forensic science international 2019 v.298 pp. 384-392
ISSN:
0379-0738
Subject:
DNA fingerprinting,   etc   ; DNA; bone resorption; diagenesis; femur; forensic sciences; histology; humans; microstructure; necropsy; nitric acid; nuclear genome; tropical soils; tropics;     Show all 14 Subjects
Abstract:
... Human bone histological analysis is a useful tool to assess post mortem diagenesis and to predict successful nuclear DNA typing of forensic material. This study is part of a series of studies developed by the authors intended to improve the understanding of post mortem diagenesis and to develop applications for DNA analysis of skeletal species from tropical soils, in order to optimize genetic and ...
DOI:
10.1016/j.forsciint.2019.03.008

16. Genetic diversity and population structure analysis of Indian garlic (Allium sativum L.) collection using SSR markers

Author:
Kumar, Mukesh; Rakesh Sharma, V.; Kumar, Vipin; Sirohi, Ujjawal; Chaudhary, Veena; Sharma, Shiveta; Saripalli, Gautam; Naresh, R. K.; Yadav, Hemant Kumar; Sharma, Shailendra
Source:
Physiology and molecular biology of plants 2019 v.25 no.2 pp. 377-386
ISSN:
0971-5894
Subject:
DNA fingerprinting,   etc   ; Allium sativum; cluster analysis; garlic; genetic variation; germplasm; microsatellite repeats; oligodeoxyribonucleotides; population structure; principal component analysis; screening;     Show all 11 Subjects
Abstract:
... Genetic diversity was assessed among 53 Indian garlic accessions using SSR markers. Initially, 24 SSR primer pairs were used for screening three selected garlic accessions. Out of 24 SSR primer pairs, 10 primer pairs which consistently showed good amplification and polymorphism were selected for DNA profiling. SSR primer pairs showed PIC values ranging from 0.30 to 0.99. Based on AMOVA we found th ...
DOI:
10.1007/s12298-018-0628-y

17. Is DNA fingerprinting the gold standard for estimation of adoption and impacts of improved lentil varieties?

Author:
Yigezu, Yigezu A.; Alwang, Jeffrey; Rahman, M. Wakilur; Mollah, M. Bazlur R.; El-Shater, Tamer; Aw-Hassan, Aden; Sarker, Ashutosh
Source:
Food policy 2019 v.83 pp. 48-59
ISSN:
0306-9192
Subject:
DNA fingerprinting,   etc   ; blight; breeding; cooking; disease susceptibility; experts; household surveys; households; landraces; lentils; regression analysis; relay cropping; taste; Bangladesh;     Show all 14 Subjects
Abstract:
... In the early 1980s, disease susceptibility in short-season lentil landraces began to limit productivity in areas where relay cropping took place in Bangladesh. Since then, several improved high-yielding lentil varieties, which are resistant to rust and blight and suitable in the relay cropping system, have been released jointly by national and international research centers. This study used three ...
DOI:
10.1016/j.foodpol.2018.11.004

18. Molecular characterization of Ralstonia solanacearum strains causing bacterial wilt of solanaceous crops in Myanmar by rep-PCR analysis

Author:
Kyaw, Htet Wai Wai; Tsuchiya, Kenichi; Matsumoto, Masaru; Aye, Seint San; Iiyama, Kazuhiro; Kurose, Daisuke; Horita, Mitsuo; Furuya, Naruto
Source:
Journal of general plant pathology 2019 v.85 no.1 pp. 33-38
ISSN:
1345-2630
Subject:
DNA fingerprinting,   etc   ; Ralstonia solanacearum; bacterial wilt; cluster analysis; crops; genetic variation; host plants; phylotype; polymerase chain reaction; provenance; Myanmar;     Show all 11 Subjects
Abstract:
... Seventy strains of Ralstonia solanacearum from solanaceous crops in Myanmar were molecularly characterized by rep-PCR DNA fingerprinting. Cluster analysis based on the fingerprints (total 33 fingerprint types) revealed seven main clusters at 80% similarity level. Strains in phylotype I showed 29 fingerprint types and were divided into six clusters, most of which were closely correlated with host p ...
DOI:
10.1007/s10327-018-0818-z

19. Application of next generation sequencing for species identification in meat and poultry products: A DNA metabarcoding approach

Author:
Xing, Ran-Ran; Wang, Nan; Hu, Ran-Ran; Zhang, Jiu-Kai; Han, Jian-Xun; Chen, Ying
Source:
Food control 2019 v.101 pp. 173-179
ISSN:
0956-7135
Subject:
DNA fingerprinting,   etc   ; DNA barcoding; birds; databases; food fraud; high-throughput nucleotide sequencing; mammals; mitochondrial genes; poultry products; processed meat; ribosomal RNA; species diversity; species identification; China;     Show all 14 Subjects
Abstract:
... Species substitution is one of the forms of food fraud for economic gain. The vast majority of existing DNA typing methods are targeted methods that developed for the determination of the specific species in food products. However, these methods are inappropriate for the analysis of unknown food products or a blend of more than one species. This study aimed to investigate the ability of high-throu ...
DOI:
10.1016/j.foodcont.2019.02.034

20. Antagonistic effects of Streptococcus and Lactobacillus probiotics in pharyngeal biofilms

Author:
Humphreys, G.J.; McBain, A.J.
Source:
Letters in applied microbiology 2019 v.68 no.4 pp. 303-312
ISSN:
0266-8254
Subject:
DNA fingerprinting,   etc   ; Lactobacillus acidophilus; Lactobacillus plantarum; Lactococcus plantarum; Staphylococcus; Streptococcus pyogenes; Streptococcus salivarius; acidification; acids; antagonism; bacteria; biofilm; fermentation; models; pathogens; pharyngitis; pharynx; plate count; probiotics; throat; viability;     Show all 21 Subjects
Abstract:
... Direct antagonism towards pathogens including Streptococcus pyogenes is a proposed mechanism of pharyngeal probiosis but off‐target effects on the symbiotic microbiota of the throat are possible and may be beneficial, harmful or neutral. We have assessed the bacteriological effects of two candidate Lactobacillus probiotics and the established pharyngeal probiotic Streptococcus salivarius K12. Anta ...
DOI:
10.1111/lam.13133