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- Daniels, Greg, et al. Show all 8 Authors
- Canadian journal of plant pathology 2018 v.40 no.3 pp. 417-422
- DNA; Fusarium graminearum; denaturation; nucleic acid annealing; oligodeoxyribonucleotides; polymerase chain reaction; protocols; quality control; wheat
- ... A simple and accurate PCR protocol for identification of Fusarium graminearum from fungal cultures was developed. In this protocol, DNA extraction was omitted by using the Thermo Scientific Phire direct PCR master mix kit for template preparation and PCR. Two primer pairs, ITS5/ITS2 for DNA template quality control and Fg16F/Fg16R for F. graminearum identification, were used in a duplex PCR. Each ...