PubAg
Main content area
Limit your search
Search
25 Search Results
« Previous |
1 - 20 of 25
|
Next »
Search Results
- Author:
- David P. Alt, et al. ; Jarlath E. Nally; Richard L. Hornsby; Show all 3 Authors
- Source:
- Veterinary sciences 2021 v.8 no.9 pp. -
- ISSN:
- 2306-7381
- Subject:
- cattle; cell-mediated immunity; excretion; leptospirosis; morbidity; mortality; rats; serotypes; urine; wildlife; zoonoses
- Abstract:
- ... Domestic and wildlife animal species act as reservoir hosts of leptospirosis, a global zoonotic disease affecting more than 1 million people annually and causing significant morbidity and mortality in domestic animals. In contrast to incidental hosts which present with an array of clinical manifestations, reservoir hosts are typically asymptomatic and can shed leptospires from chronically infected ...
- DOI:
- 10.3390/vetsci8090178
- https://doi.org/10.3390/vetsci8090178
- Author:
- David P. Alt, et al. ; Ellie J. Putz; Luis G.V. Fernandes; Sathesh K. Sivasankaran; Darrell O. Bayles; John D. Lippolis; Jarlath E. Nally; Show all 7 Authors
- Source:
- Journal of proteomics 2022 v.262 pp. 104602
- ISSN:
- 1874-3919
- Subject:
- Leptospira borgpetersenii subsp. hardjo; body temperature; cattle; cold; cross immunity; death; gene expression; leptospirosis; people; protein synthesis; proteome; proteomics; serotypes; slaughterhouses; transcriptome; vaccine development; vaccines; wildlife; zoonoses
- Abstract:
- ... Leptospirosis is a global zoonotic disease affecting humans and livestock species. Bacterin vaccines lack cross protection between serogroups, and include multiple serovars propagated at 29 °C. Recent work demonstrated substantial variation in the transcriptome of identical species and serovars of Leptospira. Here, substantial differences in protein abundance profiles were identified in Leptospira ...
- Handle:
- 10113/7767080
- DOI:
- 10.1016/j.jprot.2022.104602
- CHORUS:
- 10.1016/j.jprot.2022.104602
- https://doi.org/10.1016/j.jprot.2022.104602
3. Phenotypic and proteomic characterization of treponemes associated with bovine digital dermatitis
- Author:
- David P. Alt, et al. ; Jarlath E. Nally; Richard L. Hornsby; Julian P. Whitelegge; Show all 4 Authors
- Source:
- Veterinary microbiology 2019 v.235 pp. 35-42
- ISSN:
- 0378-1135
- Subject:
- ABC transporters; ambient temperature; cooked foods; dairy cows; dermatitis; diagnostic techniques; infectious diseases; lipoproteins; mass spectrometry; microscopy; outer membrane proteins; phenotype; phylotype; protein composition; protein synthesis; proteomics; signal peptide; two-dimensional gel electrophoresis; vaccine development; Iowa
- Abstract:
- ... Bovine digital dermatitis (BDD) is a multifactorial polymicrobial infectious disease associated with multiple species and phylotypes of treponemes. However, despite the abundance of molecular signatures for treponemes that are identified in bovine lesions, relatively few isolates are cultured, and even fewer have been characterized at the level of protein expression. Here we report the successful ...
- DOI:
- 10.1016/j.vetmic.2019.05.023
- PubMed:
- 31282377
- PubMed Central:
- PMC6684396
- https://doi.org/10.1016/j.vetmic.2019.05.023
- Author:
- David P. Alt, et al. ; Matthew G. Bakker; Torey Looft; Kathleen Delate; Cynthia A. Cambardella; Show all 5 Authors
- Source:
- Canadian journal of microbiology 2018 v.64 no.12 pp. 901-914
- ISSN:
- 1480-3275
- Subject:
- agricultural management; alfalfa; community structure; corn; genes; greenhouse gases; management systems; methane monooxygenase (particulate); methane monooxygenase (soluble); microbiome; nitrous-oxide reductase; pH; prokaryotic cells; soil; soil bacteria; soil quality
- Abstract:
- ... Understanding how soil microbiomes respond to management is essential to maximizing soil health. We contrasted microbiomes in bulk soil under long-term organic and conventional management in a grain production setting. Management category significantly impacted the relative abundances of 17% of the most abundant taxa. Both conventional and organic management favored particular taxa, but these effe ...
- Handle:
- 10113/6440083
- DOI:
- 10.1139/cjm-2018-0134
- https://doi.org/10.1139/cjm-2018-0134
- Author:
- David P. Alt, et al. ; Jennifer H. Wilson-Welder; Jarlath E. Nally; Samuel B. Humphrey; Steven C. Olsen; Show all 5 Authors
- Source:
- Journal of dairy science 2018 v.101 no.9 pp. 8301-8307
- ISSN:
- 0022-0302
- Subject:
- B-lymphocytes; CD4-positive T-lymphocytes; antibodies; bacterial antigens; blood serum; cattle diseases; dairy cattle; dermatitis; flow cytometry; hoof trimming; immune response; infectious diseases; interferon-gamma; lameness; pathogens; phenotype; secretion
- Abstract:
- ... Digital dermatitis is an infectious disease of cattle and the leading cause of lameness. This disease is complicated by the reoccurrence of the lesions and the observation of lesions on more than one limb at different time points, indicating infection may not result in a protective immune response. The objective of this study was to characterize the peripheral blood cellular response in naturally ...
- DOI:
- 10.3168/jds.2017-13913
- https://doi.org/10.3168/jds.2017-13913
- Author:
- David P. Alt, et al. ; Jennifer H. Wilson-Welder; Paola Boggiatto; Jarlath E. Nally; Emad I. Wafa; Richard L. Hornsby; Ami Frank; Douglas E. Jones; Steven C. Olsen; Ned B. Bowden; Aliasger K. Salem; Show all 11 Authors
- Source:
- Vaccine 2020 v.38 no.18 pp. 3464-3473
- ISSN:
- 0264-410X
- Subject:
- Leptospira borgpetersenii subsp. hardjo; T-lymphocytes; aluminum hydroxide; bacterial antigens; biodegradability; blood serum; cattle; cattle diseases; cattle production; emulsions; financial economics; humoral immunity; immune response; immunogenicity; immunoglobulin G; inoculum; interferon-gamma; interleukin-17; kidneys; leptospirosis; microparticles; phenotype; polymers; serotypes; urine; vaccination; vaccine adjuvants; vaccines; zoonoses
- Abstract:
- ... Leptospirosis is a global zoonosis causing significant economic losses for cattle production. Current cattle vaccines against leptospirosis need improvement to provide efficacy against multiple serovars, reduce shedding in urine, and to induce earlier and more robust immune responses. In this study, Leptospira borgpetersenii serovar Hardjo strain 203 antigen was combined with novel adjuvants (a bi ...
- DOI:
- 10.1016/j.vaccine.2020.02.086
- CHORUS:
- 10.1016/j.vaccine.2020.02.086
- https://doi.org/10.1016/j.vaccine.2020.02.086
7. Experimental Transmission of Bovine Digital Dermatitis to Sheep: Development of an Infection Model
- Author:
- David P. Alt, et al. ; Jennifer H. Wilson-Welder; Jarlath E. Nally; Mitchell V. Palmer; John Coatney; Paul Plummer; Show all 6 Authors
- Source:
- Veterinary pathology v.55 no.2 pp. 245-257
- ISSN:
- 1544-2217
- Subject:
- Treponema; anaerobes; animal models; animal pathology; bacteria; biopsy; cattle; crossbreds; dermatitis; dermis; diagnostic techniques; elks; environmental factors; etiology; feet; flocks; foot diseases; goats; host-pathogen relationships; hyperkeratosis; keratinocytes; lameness; sheep; silver staining
- Abstract:
- ... Digital dermatitis is an infectious cause of lameness primarily affecting cattle but also described in sheep, goats, and wild elk. Digital dermatitis is a polymicrobial infection, involving several Treponema species and other anaerobic bacteria. Although the exact etiology has not been demonstrated, a number of bacterial, host, and environmental factors are thought to contribute to disease develop ...
- DOI:
- 10.1177/0300985817736572
- https://doi.org/10.1177/0300985817736572
- Author:
- David P. Alt, et al. ; Sushan Han; Kristin G. Mansfield; Dan S. Bradway; Thomas E. Besser; Deryck H. Read; Gary J. Haldorson; Jennifer H. Wilson-Welder; Show all 8 Authors
- Source:
- Veterinary pathology 2019 v.56 no.1 pp. 118-132
- ISSN:
- 1544-2217
- Subject:
- Cervus elaphus; Treponema; adults; anaerobes; animal pathology; antibodies; bacteria; calves; dermatitis; elks; enzootic diseases; enzyme-linked immunosorbent assay; epithelium; hooves; hyperplasia; immunohistochemistry; inflammation; pathogenesis; polymerase chain reaction; sheep; sole ulcers; Washington (state)
- Abstract:
- ... A novel foot disease in free-ranging elk (Cervus elaphus) in southwestern Washington State emerged in 2008 and spread throughout the region. Initial studies showed adult elk had chronic hoof overgrowth, sole ulcers, and sloughed hoof capsules, but no cause was determined. To identify possible causes and characterize the earliest lesions, 9-, 7-, and 3-month-old elk were collected. Nine-month-old e ...
- DOI:
- 10.1177/0300985818798108
- CHORUS:
- 10.1177/0300985818798108
- https://doi.org/10.1177/0300985818798108
- Author:
- David P. Alt, et al. ; Jennifer H. Wilson-Welder; Jarlath E. Nally; Show all 3 Authors
- Source:
- Veterinary medicine: research and reports 2015 v.2015 no.6 pp. 155-164
- ISSN:
- 2230-2034
- Subject:
- beef cattle; dairy cattle; dermatitis; disease course; disease transmission; elks; etiology; feet; goats; infectious diseases; inflammation; lesions (animal); sheep
- Abstract:
- ... Digital dermatitis (DD) is a multifactorial polymicrobial infectious disease originally described in dairy cattle, but is increasingly recognized in beef cattle, sheep, and more recently, elk and goats. Clinical bovine lesions typically appear on the plantar surface of the hind foot from the interdigital space and heel bulb to the accessory digits, with a predilection for skin–horn junctions. Lesi ...
- Handle:
- 10113/60926
- DOI:
- 10.2147/VMRR.S62072
- PubMed:
- 30101102
- PubMed Central:
- PMC6070020
- https://doi.org/10.2147/VMRR.S62072
- Author:
- David P. Alt, et al. ; Jarlath E. Nally; Richard L. Hornsby; Darrell Bayles; Jennifer H. Wilson-Welder; Debra E. Palmquist; Nathan E. Bauer; Show all 7 Authors
- Source:
- Veterinary microbiology 2018 v.218 pp. 25-30
- ISSN:
- 0378-1135
- Subject:
- Leptospira borgpetersenii; agglutination tests; animal models; beef cattle; bladder; blood serum; cross-sectional studies; disease reservoirs; excretion; fluorescent antibody technique; renal tubules; ribosomal DNA; sequence analysis; serology; seroprevalence; serotypes; slaughterhouses; urine; virulence
- Abstract:
- ... Pathogenic leptospires colonize the renal tubules of reservoir hosts of infection, including cattle, and are excreted via urine. In order to identify circulating serovars of pathogenic leptospires in beef cattle, and their associated rates of urinary excretion, a cross sectional study was performed. Fifty urine samples were collected one day each month over 12 consecutive months (N = 600), directl ...
- DOI:
- 10.1016/j.vetmic.2018.03.023
- https://doi.org/10.1016/j.vetmic.2018.03.023
- Author:
- David P. Alt, et al. ; Kathy T. Mou; Heather K. Allen; Julian Trachsel; Samantha J. Hau; Johann F. Coetzee; Devin B. Holman; Steven Kellner; Crystal L. Loving; Susan L. Brockmeier; Show all 10 Authors
- Source:
- Veterinary microbiology 2019 pp. -
- ISSN:
- 0378-1135
- Subject:
- Actinobacillus; Streptococcus; acute exposure; bacterial communities; community structure; intramuscular injection; microorganisms; necropsy; nose; oxytetracycline; swine; tonsils
- Abstract:
- ... The impacts of antibiotic treatment and dosing regimen of an antibiotic on the swine respiratory microbiota are poorly defined. To begin to address this, this study characterized the impact of oxytetracycline administration, given either parenterally or in feed, on the diversity of the nasal and tonsil microbiotas of post-weaned pigs over a two-week period. One group received a single intramuscula ...
- DOI:
- 10.1016/j.vetmic.2019.108386
- CHORUS:
- 10.1016/j.vetmic.2019.108386
- https://doi.org/10.1016/j.vetmic.2019.108386
- Author:
- David P. Alt, et al. ; Jennifer H. Wilson-Welder; Jarlath E. Nally; Show all 3 Authors
- Source:
- Animals 2015 v.5 no.4 pp. 1114-1135
- ISSN:
- 2076-2615
- Subject:
- antibodies; blood serum; dairy cattle; dermatitis; etiology; immune response; lameness; phylotype
- Abstract:
- ... Globally; digital dermatitis is a leading form of lameness observed in production dairy cattle. While the precise etiology remains to be determined; the disease is clearly associated with infection by numerous species of treponemes; in addition to other anaerobic bacteria. The goal of this review article is to provide an overview of the current literature; focusing on discussion of the polybacteri ...
- DOI:
- 10.3390/ani5040400
- PubMed:
- 26569318
- PubMed Central:
- PMC4693204
- https://doi.org/10.3390/ani5040400
- Author:
- David P. Alt, et al. ; Jennifer Wilson-Welder; Show all 2 Author
- Source:
- Biologicals 2013 v.41 pp. 323-324
- ISSN:
- 1045-1056
- Subject:
- hamsters; recombinant antigens; Leptospira interrogans serovar Hardjo; hosts; Leptospira borgpetersenii; recombinant proteins; models; serotypes; vaccines; immune response; vaccination; case studies; Leptospira interrogans serovar Icterohaemorrhagiae
- Abstract:
- ... Evaluation of leptospiral vaccines for potency against Leptospira interrogans serovars Pomona, Icterohaemorrhagiae, Canicola, and Grippotyphosa is accomplished using the hamster potency test method described in 9 CFR 113.101-104. Applicability of this method to evaluation of bacterins developed for immunization against infection with L. interrogans serovar Hardjo or Leptospira borgpetersenii serov ...
- Handle:
- 10113/57664
- DOI:
- 10.1016/j.biologicals.2013.06.003
- https://doi.org/10.1016/j.biologicals.2013.06.003
- Author:
- David P. Alt, et al. ; Andrew K. Cheung; Terry Fei Fan Ng; Kelly M. Lager; Eric Delwart; Roman M. Pogranichniy; Show all 6 Authors
- Source:
- Archives of virology 2015 v.160 no.1 pp. 353-358
- ISSN:
- 0304-8608
- Subject:
- DNA replication; circular DNA; coat proteins; feces; genome; intergenic DNA; metagenomics; monophyly; open reading frames; ssDNA viruses; swine; viruses
- Abstract:
- ... Metagenomic analysis of fecal samples collected from diarrheal swine detected sequences encoding a replication initiator protein (Rep). The genomes of ten novel single-stranded DNA viruses were determined, and they exhibited a similar genome organization. The two putative open reading frames (ORFs) encoding Rep and the capsid protein are bidirectionally transcribed and separated by two intergenic ...
- DOI:
- 10.1007/s00705-014-2234-9
- PubMed:
- 25248627
- https://doi.org/10.1007/s00705-014-2234-9
- Author:
- David P. Alt, et al. ; Jennifer H. Wilson-Welder; Margaret K. Elliott; Richard L. Zuener; Darrell O. Bayles; Thad B. Stanton; Show all 6 Authors
- Source:
- BMC Microbiology 2013 v.13 no.280 pp. 1-9
- ISSN:
- 1471-2180
- Subject:
- Treponema phagedenis; acetates; amino acid sequences; bacterial infections; butyrates; dairy cows; dermatitis; etiology; fermentation; flagellum; formic acid; fructose; genitalia; genome; genomics; glucose; humans; lameness; lesions (animal); maltose; mannitol; mannose; nucleic acid hybridization; nucleotide sequences; pH; pectins; ribose; sequence analysis; temperature; volatile fatty acids; Iowa
- Abstract:
- ... Background: Bovine papillomatous digital dermatitis (DD) is the leading cause of lameness in dairy cattle and represents a serious welfare and economic burden. Found primarily in high production dairy cattle worldwide, DD is characterized by the development of an often painful red, raw ulcerative or papillomatous lesion frequently located near the interdigital cleft and above the bulbs of the heel ...
- Handle:
- 10113/58913
- DOI:
- 10.1186/1471-2180-13-280
- PubMed:
- 24304812
- PubMed Central:
- PMC3879011
- https://doi.org/10.1186/1471-2180-13-280
- Author:
- David P. Alt, et al. ; Torey Looft; Heather K. Allen; Brandi L. Cantarel; Uri Y. Levine; Darrell O. Bayles; Bernard Henrissat; Thaddeus B. Stanton; Show all 8 Authors
- Source:
- Journal of the International Society for Microbial Ecology 2014 v.8 pp. 1566-1576
- ISSN:
- 1751-7370
- Subject:
- Escherichia; Lachnobacterium; bacteria; bacterial communities; bacteriophages; cecum; cell wall components; chlortetracycline; colon; feces; feed conversion; food safety; genes; ileum; inoculum; intestinal microorganisms; metagenomics; mucosa; nucleotide sequences; penicillins; ribosomal RNA; swine
- Abstract:
- ... Disturbance of the beneficial gut microbial community is a potential collateral effect of antibiotics, which have many uses in animal agriculture (disease treatment or prevention and feed efficiency improvement). Understanding antibiotic effects on bacterial communities at different intestinal locations is essential to realize the full benefits and consequences of in-feed antibiotics. In this stud ...
- Handle:
- 10113/58451
- DOI:
- 10.1038/ismej.2014.12
- PubMed:
- 24522263
- PubMed Central:
- PMC4817603
- https://doi.org/10.1038/ismej.2014.12
- Author:
- David P. Alt, et al. ; Qingzhong Wu; Katherine C. Prager; Tracey Goldstein; Renee L. Galloway; Richard L. Zuerner; James O. Lloyd-Smith; Lori Schwacke; Show all 8 Authors
- Source:
- Veterinary microbiology 2014 v.110 no.3 pp. 165-172
- Subject:
- DNA; DNA primers; Leptospira biflexa; Leptospira interrogans; Leptospira meyeri; Zalophus; detection limit; genes; kidneys; leptospirosis; microbial detection; pathogens; quantitative polymerase chain reaction; serotypes; urine
- Abstract:
- ... Several real-time PCR assays are currently used for detection of pathogenic Leptospira spp.; however, few methods have been described for the successful evaluation of clinical urine samples. This study reports a rapid assay for the detection of pathogenic Leptospira spp. in California sea lions Zalophus californianus using real-time PCR with primers and a probe targeting the lipL32 gene. The PCR a ...
- Handle:
- 10113/59506
- DOI:
- 10.3354/dao02752
- PubMed:
- 25114040
- PubMed Central:
- PMC6423441
- https://doi.org/10.3354/dao02752
- Author:
- David P. Alt, et al. ; Kelly M. Lager; Terry F. Ng; Darrell O. Bayles; Eric L. Delwart; Andrew K. Cheung; Show all 6 Authors
- Source:
- Journal of veterinary diagnostic investigation v.24 no.6 pp. 1177-1179
- ISSN:
- 1943-4936
- Subject:
- experimental design; feces; high-throughput nucleotide sequencing; nucleic acids; parturition; pathogens; scientists; swine; viruses
- Abstract:
- ... Although advances in nucleic acid sequencing have enabled the discovery of many infectious agents, challenges remain for scientists and veterinary diagnosticians trying to design animal studies with a minimum of variables and to interpret laboratory results. To evaluate pyrosequencing technology as a potential screening method to estimate the virome in pigs, fecal samples were collected from 4 pig ...
- DOI:
- 10.1177/1040638712463212
- PubMed:
- 23051826
- https://doi.org/10.1177/1040638712463212
- Author:
- David P. Alt, et al. ; Jennifer H. Wilson-Welder; Darrell O. Bayles; Caroline Cameron; Ben Adler; Dieter M. Bulach; Torsten Seemann; Michael J. Lehane; Lee R. Haines; Alistair C. Darby; Neil Hall; Alan D. Radford; Richard L. Zuerner; Show all 13 Authors
- Source:
- Genome Announcements 2015 v.3 no.3 pp. e00678-15
- ISSN:
- 2169-8287
- Subject:
- Leptospira interrogans; chromosomes; genome assembly; horses; nucleotide sequences; reproductive performance; serotypes; swine; wildlife
- Abstract:
- ... Leptospira interrogans serovar Bratislava infection occurs in multiple domestic and wildlife species and is associated with poor reproductive performance in swine and horses. We present the complete genome assembly of strain PigK151 comprising two chromosomes, CI (4.457 Mbp) and CII (358 kbp). ...
- DOI:
- 10.1128/genomeA.00678-15
- PubMed:
- 26112787
- PubMed Central:
- PMC4481285
- https://doi.org/10.1128/genomeA.00678-15
- Author:
- David P. Alt, et al. ; Baoqing Guo; Ann C. Vorwald; Kelly M. Lager; Darrell O. Bayles; Kay S. Faaberg; Show all 6 Authors
- Source:
- Virus research 2011 v.161 no.2 pp. 162-169
- ISSN:
- 0168-1702
- Subject:
- sequence analysis; porcine reproductive and respiratory syndrome; Porcine reproductive and respiratory syndrome virus; genome; amino acid sequences; amino acids; viruses; mutants; nucleotide sequences; point mutation; swine
- Abstract:
- ... Fifteen porcine reproductive and respiratory syndrome virus (PRRSV) isolate genomes were derived simultaneously using 454 pyrosequencing technology. The viral isolates sequenced were from a recent swine study, in which engineered Type 2 prototype PRRSV strain VR-2332 mutants, with 87, 184, 200, and 403 amino acid deletions in the second hypervariable region of nsp2, were found to be stable in the ...
- Handle:
- 10113/50425
- DOI:
- 10.1016/j.virusres.2011.07.018
- https://doi.org/10.1016/j.virusres.2011.07.018