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- Defoor, Els, et al. Show all 4 Authors
- Applied and environmental microbiology 2008 v.74 no.15 pp. 4772-4775
- Escherichia coli; Lactococcus lactis; auxotrophs; bacteriophages; chromosomes; erythromycin; gene expression; genes; homologous recombination; lactic acid bacteria; toxicity; transporters; triose-phosphate isomerase
- ... In this paper we describe the new selection/counterselection vector pCS1966, which is suitable for both sequence-specific integration based on homologous recombination and integration in a bacteriophage attachment site. This plasmid harbors oroP, which encodes a dedicated orotate transporter, and can replicate only in Escherichia coli. Selection for integration is performed primarily by resistance ...