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... Diarrheagenic Escherichia coli is a heterogeneous group of strains that presents various virulence factors and causes different diarrheal syndromes. The most studied pathotypes are enteropathogenic Escherichia coli (EPEC), enterotoxigenic Escherichia coli (ETEC), enteroaggregative Escherichia coli (EAEC), enteroinvasive Escherichia coli (EIEC) and Shiga toxin-producing Escherichia coli (STEC). The ...
... Here, we report a new protocol for enhancing the soluble expression of inclusion body (IB)-prone proteins in E. coli using nanobodies (Nbs) as a molecular-specific chaperone. The specific intracellular binding between the cognate-Nbs and the antigen is successfully achieved and enables the formation of a soluble Nb–antigen complex in E. coli. We further expand this method by adding an epitope tag ...
... To maintain proteostasis, the cell employs multiple ribosome rescue systems to relieve the stalled ribosome on problematic mRNA. One example of problematic mRNA is non-stop mRNA that lacks an in-frame stop codon produced by endonucleolytic cleavage or transcription error. In Escherichia coli, there are at least three ribosome rescue systems that deal with the ribosome stalled on non-stop mRNA. Acc ...
Escherichiacoli, etc ; Ziziphus jujuba; biosynthesis; glycosides; Show all 4 Subjects
Abstract:
... A novel and efficient 2′′-O-glycosyltransferase ZjOGT38 was identified from Ziziphus jujuba. It could regio-selectively glycosylate 2-hydroxyflavanone C-glycosides. ZjOGT38 allowed de novo biosynthesis of isovitexin 2′′-O-glucoside in E. coli. ...
Escherichiacoli, etc ; hydrophilicity; porosity; ultraviolet radiation; Show all 4 Subjects
Abstract:
... Inspired by the stomatal feature of plant leaves, a photo-responsive membrane was developed to enhance the removal of irreversible membrane fouling and to control molecule release. Photo-responsive polymers were prepared by reacting the amine group of 4-amineazobenzene with about 3, 5 and 9 out of 12 carboxylic groups of PMAA which was grafted from P(VDF-CTFE) with a certain length. Subsequently, ...
Escherichiacoli, etc ; geometry; phase-contrast microscopy; Show all 3 Subjects
Abstract:
... Understanding the behavior of bacteria at the proximity of different surfaces is of great importance and interest. Despite recent exciting progress in geometric control of bacterial behavior around surfaces, a detailed comparison on the interaction of bacteria with cylindrical surfaces of different geometric modifications is still missing. Here, we investigated how bacteria interacted with cylindr ...
Escherichiacoli, etc ; cellulose; exposure duration; paper; Show all 4 Subjects
Abstract:
... CuO nanoflakes with dimension of 50–150 nm × 200 nm were successfully immobilized on the surface of cellulose fibers by an ultrafast (5 min reaction time), scalable microwave assisted method. The high retention of CuO up to 70% has been phenomenal considering that the immobilization was carried out in the absence of any linker, binder or retention aid. Retention of almost 87% of the immobilized Cu ...
Escherichiacoli, etc ; gel electrophoresis; reporter genes; Show all 3 Subjects
Abstract:
... Amino acid transport systems perform important physiological functions; their role should certainly be considered in microbial production of amino acids. Typically, in the context of metabolic engineering, efforts are focused on the search for and application of specific amino acid efflux pumps. However, in addition, importers can also be used to improve the industrial process as a whole. In this ...
enteropathogenic Escherichiacoli; enterotoxigenic Escherichiacoli, etc ; Carica papaya; antibacterial properties; death; diarrhea; drugs; gastrointestinal system; public health; serotypes; therapeutics; Show all 11 Subjects
Abstract:
... Background: Diarrhea is currently the second leading cause of death among children under five years old in the world and therefore, remains a global public health problem. Considering that most cases of diarrhea are due to gastrointestinal infections, and medicinal plants have been traditionally used to treat such conditions, this work aimed to evaluate the chemical composition, antibacterial acti ...
Escherichiacoli, etc ; acetates; byproducts; energy; metabolism; transcriptomics; Show all 6 Subjects
Abstract:
... A novel Escherichia coli efficient expression system had been constructed in our previous study. The system was based on the overexpression of endogenous genes prpD and malK to enhance the expression of exogenous genes. In this study, a general regulatory mechanism of prpD and malK was first revealed through transcriptome analysis and many experimental verifications. We surprisingly proved that ov ...
Escherichiacoli, etc ; fabrics; polyesters; smoke; ultraviolet radiation; Show all 5 Subjects
Abstract:
... The exploration of novel and multifunctional coatings through composite nanomaterials design is crucial for developing next-generation polyester fabrics. Herein, we report an efficient approach of self-assembling MoO₃–ₓ QDs on MXene (Ti₃C₂Tₓ) to fabricate multifunctional polyester fabrics. In a typical preparation, MoO₃–ₓ QDs with an average size of 5 nm were uniformly distributed on the surface o ...
Escherichiacoli, etc ; biopharmaceutical industry; biopharmaceuticals; fluorescence; viability; Show all 5 Subjects
Abstract:
... The improvement of cell enumeration methods for the counting of Escherichia coli (E. coli) is important as E. coli gains in popularity as a basis for biopharmaceutical applications. In the biopharmaceutical industry, enumerating, characterizing, and dosing the accurate number of cells is imperative. In this work, we demonstrate the utilization of a chip-based image cytometer using a thin-gap, low ...
Escherichiacoli, etc ; genes; recombinant fusion proteins; research; toxicity; Show all 5 Subjects
Abstract:
... A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of β-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-β-lactamase. This bifunctional immun ...
Escherichiacoli, etc ; cytoplasm; nuclear magnetic resonance spectroscopy; urea; Show all 4 Subjects
Abstract:
... The high concentration of macromolecules in cells affects the stability of proteins and protein complexes via hard repulsions and chemical interactions, yet few studies have focused on chemical interactions. We characterized the domain-swapped dimer of the B1 domain of protein G in buffer and Escherichia coli cells by using heteronuclear, multidimensional nuclear magnetic resonance spectroscopy. I ...
Escherichiacoli, etc ; Osmanthus fragrans; biosynthesis; cultivars; flavor; lycopene; Show all 6 Subjects
Abstract:
... Sweet osmanthus (Osmanthus fragrans) flowers, as a food flavor additive, are rich in carotenoids, the majority of which are cyclized carotenoids. Lycopene β- and ε-cyclases (LCYB and LCYE, respectively) catalyze the cyclization of the open ends of acyclic lycopene and manipulate the metabolic flux into different downstream branches. Although both LCYB and LCYE have been fully characterized in many ...
Escherichiacoli, etc ; molecular biology; mutants; proteinases; uracil; yeasts; Show all 6 Subjects
Abstract:
... Natural evolution is driven by random mutations that improve fitness. In vitro evolution mimics this process, however, on a short time-scale and is driven by the given bait. Here, we used directed in vitro evolution of a random mutant library of Uracil glycosylase (eUNG) displayed on yeast surface to select for binding to chaperones GroEL, DnaK + DnaJ + ATP (DnaKJ) or E. coli cell extract (CE), us ...
Escherichiacoli, etc ; feasibility studies; hardwood; microbial contamination; softwood; Show all 5 Subjects
Abstract:
... The antimicrobial characteristics of Australian commercial timber species were studied using a direct screening method and two different microorganisms. The effectiveness of timber samples was compared with plastic and paper samples during an incubation period of 24 h at 37 °C. The initial data with E. coli and S. aureus showed no difference in performance between the plastic and paper samples and ...
Escherichiacoli, etc ; biotin; biotinylation; green fluorescent protein; immunoblotting; research; Show all 6 Subjects
Abstract:
... Proteins and antibodies labeled with biotin have been widely used for protein analysis, enzyme immunoassays, and diagnoses. Presently, they are prepared using either a chemical reaction involving a biotin N-hydroxysuccinimide (NHS) ester compound or by enzymatic biotin ligation using a combination of a biotinylation-peptide tag and Escherichia coli BirA. However, these methods are relatively compl ...
Escherichiacoli, etc ; Salmonella; bacterial contamination; decontamination; genes; microbiome; salmonellosis; Show all 7 Subjects
Abstract:
... Reduction of Salmonella on poultry carcasses is one way to prevent salmonellosis. The purpose of this research was to evaluate the effects of subzero saline chilling (SSC) with/without hot water spray (HWS) on broiler carcasses prior to chilling for bacterial reduction. Eviscerated broiler carcasses were subjected to water immersion chilling (WIC, 0% NaCl/0.5°C) or SSC (4% NaCl/−2.41°C) with/witho ...
Escherichiacoli, etc ; at-risk population; biofilm; cellulose; magnetism; pathogens; Show all 6 Subjects
Abstract:
... Pathogen and biofilm contamination in aqueous systems leave millions of people at risk of waterborne diseases. Herein, to address this issue, a green and highly efficient strategy is developed to concurrently trap and kill bacteria, eliminate the debris and the existing biofilm matrix in water environment via magnetic microparticles. The particles (TPFPs) were prepared from the in-situ deposition ...