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You searched for: Subject "Escherichia coli" Remove constraint Subject: "Escherichia coli"
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1. Primer reporte de Escherichia coli diarreogénica en población pediátrica ambulatoria con diarrea atendida en la ciudad de La Plata, Argentina

Author:
Nora Beatriz Molina; Sebastián Oderiz; Cecilia Vescina; Alejandra Córdoba; Juan Ángel Basualdo; Mónica Delfina Sparo
Source:
Revista argentina microbiología 2022 v.54 no.1 pp. 15-21
ISSN:
0325-7541
Subject:
Shiga toxin-producing Escherichia coli; enteroinvasive Escherichia coli; enteropathogenic Escherichia coli,   etc   ; Shiga-like toxin 2; diarrhea; enterotoxigenic Escherichia coli; feces; hospitals; pathotypes; virulence; Argentina;     Show all 11 Subjects
Abstract:
... Diarrheagenic Escherichia coli is a heterogeneous group of strains that presents various virulence factors and causes different diarrheal syndromes. The most studied pathotypes are enteropathogenic Escherichia coli (EPEC), enterotoxigenic Escherichia coli (ETEC), enteroaggregative Escherichia coli (EAEC), enteroinvasive Escherichia coli (EIEC) and Shiga toxin-producing Escherichia coli (STEC). The ...
DOI:
10.1016/j.ram.2021.02.006

2. Nanobodies as solubilization chaperones for the expression and purification of inclusion-body prone proteins

Author:
Guangshuai Yao; Chundong Huang; Fangling Ji; Jun Ren; Xiuna Luo; Berlin Zang; Lingyun Jia
Source:
Chemical communications 2022 v.58 no.17 pp. 2898-2901
ISSN:
1364-548X
Subject:
Escherichia coli,   etc   ; epitopes; solubilization;     Show all 3 Subjects
Abstract:
... Here, we report a new protocol for enhancing the soluble expression of inclusion body (IB)-prone proteins in E. coli using nanobodies (Nbs) as a molecular-specific chaperone. The specific intracellular binding between the cognate-Nbs and the antigen is successfully achieved and enables the formation of a soluble Nb–antigen complex in E. coli. We further expand this method by adding an epitope tag ...
DOI:
10.1039/d1cc07105j

3. Bacterial Ribosome Rescue Systems

Author:
Daisuke Kurita; Hyouta Himeno
Source:
Microorganisms 2022 v.10 no.2 pp. -
ISSN:
2076-2607
Subject:
Escherichia coli,   etc   ; ribosomes; stop codon;     Show all 3 Subjects
Abstract:
... To maintain proteostasis, the cell employs multiple ribosome rescue systems to relieve the stalled ribosome on problematic mRNA. One example of problematic mRNA is non-stop mRNA that lacks an in-frame stop codon produced by endonucleolytic cleavage or transcription error. In Escherichia coli, there are at least three ribosome rescue systems that deal with the ribosome stalled on non-stop mRNA. Acc ...
DOI:
10.3390/microorganisms10020372

4. A highly selective 2′′-O-glycosyltransferase from Ziziphus jujuba and De novo biosynthesis of isovitexin 2′′-O-glucoside

Author:
Ya-Qun Zhang; Zi-Long Wang; Zhuo Chen; Zheng-Tong Jin; Aobulikasimu Hasan; Hai-Dong Wang; Yu-Wei Sun; Xue Qiao; Yong Wang; Min Ye
Source:
Chemical communications 2022 v.58 no.15 pp. 2472-2475
ISSN:
1364-548X
Subject:
Escherichia coli,   etc   ; Ziziphus jujuba; biosynthesis; glycosides;     Show all 4 Subjects
Abstract:
... A novel and efficient 2′′-O-glycosyltransferase ZjOGT38 was identified from Ziziphus jujuba. It could regio-selectively glycosylate 2-hydroxyflavanone C-glycosides. ZjOGT38 allowed de novo biosynthesis of isovitexin 2′′-O-glucoside in E. coli. ...
DOI:
10.1039/d1cc06949g

5. Bioinspired photo-responsive membrane enhanced with “light-cleaning” feature for controlled molecule release

Author:
Qisheng Ye; Rui Wang; Saitao Yan; Baoliang Chen; Xiaoying Zhu
Source:
Journal of materials chemistry B 2022 v.10 no.14 pp. 2617-2627
ISSN:
2050-7518
Subject:
Escherichia coli,   etc   ; hydrophilicity; porosity; ultraviolet radiation;     Show all 4 Subjects
Abstract:
... Inspired by the stomatal feature of plant leaves, a photo-responsive membrane was developed to enhance the removal of irreversible membrane fouling and to control molecule release. Photo-responsive polymers were prepared by reacting the amine group of 4-amineazobenzene with about 3, 5 and 9 out of 12 carboxylic groups of PMAA which was grafted from P(VDF-CTFE) with a certain length. Subsequently, ...
DOI:
10.1039/d1tb02329b

6. Interactions of E. coli with cylindrical micro-pillars of different geometric modifications

Author:
Venkata Rao Krishnamurthi; Nathaniel Harris; Ariel Rogers; Min Zou; Yong Wang
Source:
Colloids and surfaces 2022 v.209 pp. 112190
ISSN:
0927-7765
Subject:
Escherichia coli,   etc   ; geometry; phase-contrast microscopy;     Show all 3 Subjects
Abstract:
... Understanding the behavior of bacteria at the proximity of different surfaces is of great importance and interest. Despite recent exciting progress in geometric control of bacterial behavior around surfaces, a detailed comparison on the interaction of bacteria with cylindrical surfaces of different geometric modifications is still missing. Here, we investigated how bacteria interacted with cylindr ...
DOI:
10.1016/j.colsurfb.2021.112190

7. Microwave assisted ultrafast immobilization of CuO nanostructures in paper matrices for antimicrobial applications

Author:
Sudiksha Aggrawal; Debabrata Pradhan; Paritosh Mohanty
Source:
Cellulose 2022 v.29 no.4 pp. 2633-2643
ISSN:
0969-0239
Subject:
Escherichia coli,   etc   ; cellulose; exposure duration; paper;     Show all 4 Subjects
Abstract:
... CuO nanoflakes with dimension of 50–150 nm × 200 nm were successfully immobilized on the surface of cellulose fibers by an ultrafast (5 min reaction time), scalable microwave assisted method. The high retention of CuO up to 70% has been phenomenal considering that the immobilization was carried out in the absence of any linker, binder or retention aid. Retention of almost 87% of the immobilized Cu ...
DOI:
10.1007/s10570-022-04457-1

8. The Escherichia coli Amino Acid Uptake Protein CycA: Regulation of Its Synthesis and Practical Application in l-Isoleucine Production

Author:
Christine Hook; Natalya Eremina; Petr Zaytsev; Daria Varlamova; Nataliya Stoynova
Source:
Microorganisms 2022 v.10 no.3 pp. -
ISSN:
2076-2607
Subject:
Escherichia coli,   etc   ; gel electrophoresis; reporter genes;     Show all 3 Subjects
Abstract:
... Amino acid transport systems perform important physiological functions; their role should certainly be considered in microbial production of amino acids. Typically, in the context of metabolic engineering, efforts are focused on the search for and application of specific amino acid efflux pumps. However, in addition, importers can also be used to improve the industrial process as a whole. In this ...
DOI:
10.3390/microorganisms10030647

9. HPLC-DAD-ESI-MS profile, antibacterial activity, and modulation of the activity of antibiotics by Carica papaya L. against Escherichia coli serotypes

Author:
Cícera Natalia Figueiredo Leite Gondim; Denise Aline Casimiro Bezerra; Nadghia Figueiredo Leite Sampaio; Maria Anésia Sousa de.Alencar; Roger Henrique Sousa da.Costa; Lucas Silva Abreu; Joanda Paolla Raimundo.e.Silva; Francisco Nascimento Pereira Junior; Josean Fechine Tavares; Marcelo Sobral da Silva; Jaime Ribeiro-Filho; Henrique Douglas Melo Coutinho
Source:
Phytomedicine plus 2022 v.2 no.3 pp. 100306
ISSN:
2667-0313
Subject:
enteropathogenic Escherichia coli; enterotoxigenic Escherichia coli,   etc   ; Carica papaya; antibacterial properties; death; diarrhea; drugs; gastrointestinal system; public health; serotypes; therapeutics;     Show all 11 Subjects
Abstract:
... Background: Diarrhea is currently the second leading cause of death among children under five years old in the world and therefore, remains a global public health problem. Considering that most cases of diarrhea are due to gastrointestinal infections, and medicinal plants have been traditionally used to treat such conditions, this work aimed to evaluate the chemical composition, antibacterial acti ...
DOI:
10.1016/j.phyplu.2022.100306

10. A pyruvate-centered metabolic regulation mechanism for the enhanced expression of exogenous genes in Escherichia coli

Author:
Hongchen Zheng; Wenju Shu; Xiaoping Fu; Jiahan Wang; Yifan Yang; Jianyong Xu; Hui Song; Yanhe Ma
Source:
International journal of biological macromolecules 2022 v.203 pp. 58-66
ISSN:
0141-8130
Subject:
Escherichia coli,   etc   ; acetates; byproducts; energy; metabolism; transcriptomics;     Show all 6 Subjects
Abstract:
... A novel Escherichia coli efficient expression system had been constructed in our previous study. The system was based on the overexpression of endogenous genes prpD and malK to enhance the expression of exogenous genes. In this study, a general regulatory mechanism of prpD and malK was first revealed through transcriptome analysis and many experimental verifications. We surprisingly proved that ov ...
DOI:
10.1016/j.ijbiomac.2022.01.141

11. MoO3–x QDs/MXene (Ti3C2Tx) self-assembled heterostructure for multifunctional application with antistatic, smoke suppression, and antibacterial on polyester fabric

Author:
Wangyi Zhai; Yuanming Cao; Yifei Li; Min Zheng; Zuoshan Wang
Source:
Journal of materials science 2022 v.57 no.4 pp. 2597-2609
ISSN:
0022-2461
Subject:
Escherichia coli,   etc   ; fabrics; polyesters; smoke; ultraviolet radiation;     Show all 5 Subjects
Abstract:
... The exploration of novel and multifunctional coatings through composite nanomaterials design is crucial for developing next-generation polyester fabrics. Herein, we report an efficient approach of self-assembling MoO₃–ₓ QDs on MXene (Ti₃C₂Tₓ) to fabricate multifunctional polyester fabrics. In a typical preparation, MoO₃–ₓ QDs with an average size of 5 nm were uniformly distributed on the surface o ...
DOI:
10.1007/s10853-021-06690-8

12. Rapid cell counting and viability detection method of Escherichia coli Nissle using image cytometry

Author:
Michele Perry; Mary McDonald; Anders Lund; Mrinalini Nikrad; Denise Wong; William L. Rice; Leo Li-Ying Chan
Source:
Journal of microbiological methods 2022 v.192 pp. 106381
ISSN:
0167-7012
Subject:
Escherichia coli,   etc   ; biopharmaceutical industry; biopharmaceuticals; fluorescence; viability;     Show all 5 Subjects
Abstract:
... The improvement of cell enumeration methods for the counting of Escherichia coli (E. coli) is important as E. coli gains in popularity as a basis for biopharmaceutical applications. In the biopharmaceutical industry, enumerating, characterizing, and dosing the accurate number of cells is imperative. In this work, we demonstrate the utilization of a chip-based image cytometer using a thin-gap, low ...
DOI:
10.1016/j.mimet.2021.106381

13. 3.5-scFv-β-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive

Author:
Mladen Simonovic; Thorsten Mix; Miodrag Glumac; Boris Pejin
Source:
Natural product research 2022 v.36 no.5 pp. 1317-1320
ISSN:
1478-6427
Subject:
Escherichia coli,   etc   ; genes; recombinant fusion proteins; research; toxicity;     Show all 5 Subjects
Abstract:
... A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of β-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-β-lactamase. This bifunctional immun ...
DOI:
10.1080/14786419.2020.1860977

14. A Difference between In Vitro and In-Cell Protein Dimer Formation

Author:
I-Te Chu; Claire J. Stewart; Shannon L. Speer; Gary J. Pielak
Source:
Biochemistry 2022 v.61 no.6 pp. 409-412
ISSN:
1520-4995
Subject:
Escherichia coli,   etc   ; cytoplasm; nuclear magnetic resonance spectroscopy; urea;     Show all 4 Subjects
Abstract:
... The high concentration of macromolecules in cells affects the stability of proteins and protein complexes via hard repulsions and chemical interactions, yet few studies have focused on chemical interactions. We characterized the domain-swapped dimer of the B1 domain of protein G in buffer and Escherichia coli cells by using heteronuclear, multidimensional nuclear magnetic resonance spectroscopy. I ...
DOI:
10.1021/acs.biochem.1c00780

15. Functional Characterization of Two Lycopene Cyclases from Sweet Osmanthus (Osmanthus fragrans)

Author:
Hongsheng Qing; Jiahong Chen; Lingli Jiang; Jieyu Qian; Jianxin Fu; Chao Zhang
Source:
Scientia horticulturae 2022 v.299 pp. 111062
ISSN:
0304-4238
Subject:
Escherichia coli,   etc   ; Osmanthus fragrans; biosynthesis; cultivars; flavor; lycopene;     Show all 6 Subjects
Abstract:
... Sweet osmanthus (Osmanthus fragrans) flowers, as a food flavor additive, are rich in carotenoids, the majority of which are cyclized carotenoids. Lycopene β- and ε-cyclases (LCYB and LCYE, respectively) catalyze the cyclization of the open ends of acyclic lycopene and manipulate the metabolic flux into different downstream branches. Although both LCYB and LCYE have been fully characterized in many ...
DOI:
10.1016/j.scienta.2022.111062

16. In vitro Evolution of Uracil Glycosylase Towards DnaKJ and GroEL Binding Evolves Different Misfolded States

Author:
Oran Melanker; Pierre Goloubinoff; Gideon Schreiber
Source:
Journal of molecular biology 2022 v.434 no.13 pp. 167627
ISSN:
0022-2836
Subject:
Escherichia coli,   etc   ; molecular biology; mutants; proteinases; uracil; yeasts;     Show all 6 Subjects
Abstract:
... Natural evolution is driven by random mutations that improve fitness. In vitro evolution mimics this process, however, on a short time-scale and is driven by the given bait. Here, we used directed in vitro evolution of a random mutant library of Uracil glycosylase (eUNG) displayed on yeast surface to select for binding to chaperones GroEL, DnaK + DnaJ + ATP (DnaKJ) or E. coli cell extract (CE), us ...
DOI:
10.1016/j.jmb.2022.167627

17. Investigating Antimicrobial Characteristics/Advantages of Australian Wood Species for Use in Food Packaging—A Feasibility Study

Author:
Maryam Shirmohammadi
Source:
Forests 2022 v.13 no.2 pp. -
ISSN:
1999-4907
Subject:
Escherichia coli,   etc   ; feasibility studies; hardwood; microbial contamination; softwood;     Show all 5 Subjects
Abstract:
... The antimicrobial characteristics of Australian commercial timber species were studied using a direct screening method and two different microorganisms. The effectiveness of timber samples was compared with plastic and paper samples during an incubation period of 24 h at 37 °C. The initial data with E. coli and S. aureus showed no difference in performance between the plastic and paper samples and ...
DOI:
10.3390/f13020334

18. A simple method for labeling proteins and antibodies with biotin using the proximity biotinylation enzyme TurboID

Author:
Ryouhei Shioya; Kohdai Yamada; Kohki Kido; Hirotaka Takahashi; Akira Nozawa; Hidetaka Kosako; Tatsuya Sawasaki
Source:
Biochemical and biophysical research communications 2022 v.592 pp. 54-59
ISSN:
0006-291X
Subject:
Escherichia coli,   etc   ; biotin; biotinylation; green fluorescent protein; immunoblotting; research;     Show all 6 Subjects
Abstract:
... Proteins and antibodies labeled with biotin have been widely used for protein analysis, enzyme immunoassays, and diagnoses. Presently, they are prepared using either a chemical reaction involving a biotin N-hydroxysuccinimide (NHS) ester compound or by enzymatic biotin ligation using a combination of a biotinylation-peptide tag and Escherichia coli BirA. However, these methods are relatively compl ...
DOI:
10.1016/j.bbrc.2021.12.109

19. Effects of hot water spray and sub-zero saline chilling on bacterial decontamination of broiler carcasses

Author:
I. Kang; H.C. Lee; B. Adhikari; S.D. Ha; Y.M. Kwon
Source:
Poultry science 2022 v.101 no.3 pp. 101688
ISSN:
0032-5791
Subject:
Escherichia coli,   etc   ; Salmonella; bacterial contamination; decontamination; genes; microbiome; salmonellosis;     Show all 7 Subjects
Abstract:
... Reduction of Salmonella on poultry carcasses is one way to prevent salmonellosis. The purpose of this research was to evaluate the effects of subzero saline chilling (SSC) with/without hot water spray (HWS) on broiler carcasses prior to chilling for bacterial reduction. Eviscerated broiler carcasses were subjected to water immersion chilling (WIC, 0% NaCl/0.5°C) or SSC (4% NaCl/−2.41°C) with/witho ...
DOI:
10.1016/j.psj.2021.101688

20. Positively-charged microcrystalline cellulose microparticles: Rapid killing effect on bacteria, trapping behavior and excellent elimination efficiency of biofilm matrix from water environment

Author:
Hao Luo; Yuan-Zhang Jiang; Lin Tan
Source:
Journal of hazardous materials 2022 v.424 pp. 127299
ISSN:
0304-3894
Subject:
Escherichia coli,   etc   ; at-risk population; biofilm; cellulose; magnetism; pathogens;     Show all 6 Subjects
Abstract:
... Pathogen and biofilm contamination in aqueous systems leave millions of people at risk of waterborne diseases. Herein, to address this issue, a green and highly efficient strategy is developed to concurrently trap and kill bacteria, eliminate the debris and the existing biofilm matrix in water environment via magnetic microparticles. The particles (TPFPs) were prepared from the in-situ deposition ...
DOI:
10.1016/j.jhazmat.2021.127299