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1. Correction to: A pH-responsive genetic sensor for the dynamic regulation of D-xylonic acid accumulation in Escherichia coli

Author:
Bañares, Angelo B.; Valdehuesa, Kris Niño G.; Ramos, Kristine Rose M.; Nisola, Grace M.; Lee, Won-Keun; Chung, Wook-Jin
Source:
Applied microbiology and biotechnology 2020 v.104 no.5 pp. 2273-2274
ISSN:
0175-7598
Subject:
Escherichia coli,   etc   ; pH; sensors (equipment);     Show all 3 Subjects
Abstract:
... In the published version, the y-axis data of Fig. 3c was incorrectly inserted (OD600 instead of D-xylonate (g L⁻¹) and the x-axes of Figs. 3b, 3d, 3e and 3f ended at 48 h instead of 72 h. See the correct Fig. 3 below. ...
DOI:
10.1007/s00253-020-10362-z

2. A photo-cross-linking approach to monitor protein dynamics in living cells

Author:
Miyazaki, Ryoji; Akiyama, Yoshinori; Mori, Hiroyuki
Source:
Biochimica et biophysica acta 2020 v.1864 no.2 pp. 129317
ISSN:
0304-4165
Subject:
Escherichia coli,   etc   ; biogenesis; cells; proteins;     Show all 4 Subjects
Abstract:
... Proteins, which comprise one of the major classes of biomolecules that constitute a cell, interact with other cellular factors during both their biogenesis and functional states. Studying not only static but also transient interactions of proteins is important to understand their physiological roles and regulation mechanisms. However, only a limited number of methods are available to analyze the d ...
DOI:
10.1016/j.bbagen.2019.03.003

3. Escherichia coli O-antigen capsule (group 4) is essential for serum resistance

Author:
Biran, Dvora; Rosenshine, Ilan; Ron, Eliora Z.
Source:
Research in microbiology 2020 v.171 no.2 pp. 99-101
ISSN:
0923-2508
Subject:
Escherichia coli,   etc   ; O-antigens; blood serum; serotypes;     Show all 4 Subjects
Abstract:
... Many septicemic Escherichia coli strains produce polysaccharide capsules, which are important for survival in serum. Here we show that a septicemic E. coli strain of serotype O78 produce an O-antigen capsule (group 4 capsule) and we show that this capsule is essential for serum survival. ...
DOI:
10.1016/j.resmic.2019.12.002

4. Comparison of Swabbing Solution Volume and gDNA Extraction Kits on DNA Recovery from Rigid Surface

Author:
Ok, Yeon Jeong; You, Hee Sang; Lee, Song Hee; Sung, Ho Joong; Kang, Hee-Gyoo; Hyun, Sung Hee
Source:
Indian journal of microbiology 2020 v.60 no.2 pp. 206-213
ISSN:
0046-8991
Subject:
Escherichia coli,   etc   ; DNA; Staphylococcus aureus; bacteria; cotton; polysorbates;     Show all 6 Subjects
Abstract:
... For bacteria sampling studies, various collection methods have been used to identify bacteria. To obtain accurate information about bacteria, high quality samples should be obtained. In order to obtain a high quality sample, a stable and large number of DNA copies must be collected. This study compared the efficiency of different methods of bacterial gDNA extraction and bacteria collection accordi ...
DOI:
10.1007/s12088-019-00854-6
PubMed:
32255853
PubMed Central:
PMC7105554

5. A lipid mixing assay to accurately quantify the fusion of outer membrane vesicles

Author:
Gnopo, Yehou M.D.; Putnam, David
Source:
Methods 2020 v.177 pp. 74-79
ISSN:
1046-2023
Subject:
Escherichia coli,   etc   ; chlorides; membrane fusion; mixing; phospholipids; quantitative analysis;     Show all 6 Subjects
Abstract:
... The intermixing of phospholipids from opposing bilayers, or membrane fusion, is a naturally occurring process that can be leveraged to produce hybrid vesicle systems. Optimizing the production of these hybrid vesicles requires accurate, sensitive, and quantitative methods of the lipid mixing that occurs during fusion. A fluorescence-based assay that uses octadecyl-rhodamine B chloride to measure l ...
DOI:
10.1016/j.ymeth.2019.11.009

6. Facilitating the enzymatic conversion of lysineto cadaverine in engineered Escherichia coli with metabolic regulation by genes deletion

Author:
Huang, Chih-Yu; Ting, Wan-Wen; Chen, Ying-Chun; Wu, Pong-Yee; Dong, Cheng-Di; Huang, Shih-Fang; Lin, Hung-Yi; Li, Sheng-Feng; Ng, I-Son; Chang, Jo-Shu
Source:
Biochemical engineering journal 2020 v.156 pp. 107514
ISSN:
1369-703X
Subject:
Escherichia coli,   etc   ; biochemical pathways; cadaverine; decarboxylation; genes; lysine; microorganisms; nylon;     Show all 8 Subjects
Abstract:
... Cadaverine, also known as 1,5-diaminopentane, is a biogenic amine particularly used for the synthesis of nylon. Cadaverine can be efficiently synthesized through decarboxylation of lysine in microorganisms. However, the respective metabolic pathways in Escherichia coli results in many byproducts in parallel with cadaverine synthesis via genes such as speE, speG, ygjG, and puuA. In this study, nine ...
DOI:
10.1016/j.bej.2020.107514

7. Household slow sand filters with and without water level control: continuous and intermittent flow efficiencies

Author:
Maciel, Paulo Marcos Faria; Sabogal-Paz, Lyda Patricia
Source:
Environmental technology 2020 v.41 no.8 pp. 944-958
ISSN:
1479-487X
Subject:
Escherichia coli,   etc   ; filtration; kaolinite; poly(vinyl chloride); sand filters; turbidity;     Show all 6 Subjects
Abstract:
... Four household slow sand filters were made out of PVC and operated in continuous and intermittent flows, with and without using a float to control the maximum level of water inside the units. The efficiency was evaluated as a function of Escherichia coli reduction and turbidity in water from the study prepared with kaolinite and E. coli suspension. The correlation of the efficiencies with the foll ...
DOI:
10.1080/09593330.2018.1515988

8. Identification and characterization of methyltransferases involved in benzylisoquinoline alkaloids biosynthesis from Stephania intermedia

Author:
Zhao, Wanli; Shen, Chen; Zhu, Jinqian; Ou, Chenhui; Liu, Manyu; Dai, Wenling; Liu, Xiufeng; Liu, Jihua
Source:
Biotechnology letters 2020 v.42 no.3 pp. 461-469
ISSN:
0141-5492
Subject:
Escherichia coli,   etc   ; Stephania; alkaloids; biosynthesis; catalytic activity; genes; methylation; methyltransferases;     Show all 8 Subjects
Abstract:
... OBJECTIVES: To characterize methyltransferases involved in the biosynthesis of benzylisoquinoline alkaloids in Stephania intermedia. RESULTS: Three N-methyltransferases, SiCNMT1, SiCNMT2, SiCNMT3, and O-methyltransferase SiSOMT were identified in Stephania intermedia. Then, four methyltransferase genes were cloned into the pGEX-6P-1 vector. The recombinant vectors were transformed into Escherichia ...
DOI:
10.1007/s10529-019-02785-0

9. Ultrasound-accelerated green synthesis of new quinolin-2-thione derivatives and antimicrobial evaluation against Escherichia coli and Staphylococcus aureus

Author:
Geesi, Mohammed H.; Moustapha, Moustapha E.; Bakht, Mohammed A.; Riadi, Yassine
Source:
Sustainable chemistry and pharmacy 2020 v.15 pp. 100195
ISSN:
2352-5541
Subject:
Escherichia coli,   etc   ; Staphylococcus aureus; antibacterial properties; green chemistry; ultrasonic treatment;     Show all 5 Subjects
Abstract:
... An efficient, green strategy to access to a new series of quinolinthiones was investigated. The targeted derivatives have been prepared from arylhydrazides and 2-thiocoumarin under ultrasound irradiation in refluxing water. The remarkable features of this strategy are simple procedure, high conversion, cleaner with short reaction times. All resultant compounds were screened against bacterial strai ...
DOI:
10.1016/j.scp.2019.100195

10. Multidrug-Resistant Escherichia coli Strain Isolated from Swine in China Harbors mcr-3.1 on a Plasmid of the IncX1 Type That Cotransfers with mcr-1.1

Author:
Sun, Dewei; Jin, Shanshan; Wang, Jing; Wang, Zhenyu; Fan, Jiajian; Xu, Zhengzhong; Xu, Yaohui; Chen, Xiang; Jiao, Xin'an
Source:
Foodborne pathogens & disease 2020
ISSN:
1556-7125
Subject:
Escherichia coli,   etc   ; multiple drug resistance; plasmids; swine; transposases; transposons; China;     Show all 7 Subjects
Abstract:
... An Escherichia coli strain isolated from the feces of swine at a pork slaughterhouse in Henan province China was found to possess two colistin-resistance genes, mcr-1 and mcr-3, plus 16 additional resistance genes. Genes mcr-1.1 and mcr-3.1 were identified on IncHI2 and IncX1 type plasmids, respectively. Transconjugants (containing mcr-3, mcr-1&mcr-3) were obtained that were 64- and 512-fold highe ...
DOI:
10.1089/fpd.2019.2769

11. Characterization of Photophysical Properties of Photoactivatable Fluorescent Proteins for Super-Resolution Microscopy

Author:
Tao, Antai; Zhang, Rongjing; Yuan, Junhua
Source:
TheJournal of physical chemistry 2020 v.124 no.10 pp. 1892-1897
ISSN:
1520-5207
Subject:
Escherichia coli,   etc   ; fluorescent proteins; image analysis; microscopy; physical chemistry;     Show all 5 Subjects
Abstract:
... Photoactivatable fluorescent proteins (PA-FPs) are widely used in photoactivated localization microscopy (PALM), and information about their blinking properties is important for precise assembling of PALM images and for quantitative molecular counting with PALM. As the photophysical properties of fluorescent proteins depend sensitively on the local physiochemical environment, we developed a series ...
DOI:
10.1021/acs.jpcb.9b11028

12. De novo biosynthesis of indole-3-ethanol and indole-3-ethanol acetate in engineered Escherichia coli

Author:
Kong, Sijia; Zhang, Jianbi; Li, Xun; Pan, Hong; Guo, Daoyi
Source:
Biochemical engineering journal 2020 v.154 pp. 107432
ISSN:
1369-703X
Subject:
Escherichia coli,   etc   ; acetates; acetyltransferases; alcohols; biosynthesis; carbon; glucose; tryptophan;     Show all 8 Subjects
Abstract:
... Indole-3-ethanol is an important pharmaceutical intermediate that possess significant pharmaceutical properties. In this study, we established an artificial pathway in engineered Escherichia coli for microbial production of indole-3-ethanol from simple carbon sources. The overall pathway includes the upstream pathway from glucose to L-tryptophan and the downstream pathway from L-tryptophan to indo ...
DOI:
10.1016/j.bej.2019.107432

13. Fermentative production of sulfur-containing amino acid with engineering putative l-cystathionine and l-cysteine uptake systems in Escherichia coli

Author:
Yamazaki, Shunsuke; Ziyatdinov, Mikhail Kharisovich; Nonaka, Gen
Source:
Journal of bioscience and bioengineering 2020
ISSN:
1389-1723
Subject:
Escherichia coli,   etc   ; amino acid transporters; auxotrophs; cysteine; genes; sulfur; toxicity;     Show all 7 Subjects
Abstract:
... Here, proteins involved in sulfur-containing amino acid uptake in Escherichia coli strains were investigated with the aim of applying the findings in fermentative amino acid production. A search of genes in an l-methionine auxotrophic strain library suggested YecSC as the putative transporter of l-cystathionine. l-Methionine production increased by 15% after amplification of yecSC in producer stra ...
DOI:
10.1016/j.jbiosc.2020.02.007

14. Construction of a switchable synthetic Escherichia coli for aromatic amino acids by a tunable switch

Author:
Liu, Xiaozhen; Niu, Hao; Huang, Zhaosong; Li, Qiang; Gu, Pengfei
Source:
Journal of industrial microbiology & biotechnology 2020 v.47 no.2 pp. 233-242
ISSN:
1367-5435
Subject:
Escherichia coli,   etc   ; auxotrophs; bacteria; metabolic engineering; models; phenylalanine; tryptophan; tyrosine;     Show all 8 Subjects
Abstract:
... Escherichia coli, a model microorganism for which convenient metabolic engineering tools are available and that grows quickly in cheap media, has been widely used in the production of valuable chemicals, including aromatic amino acids. As the three aromatic amino acids, L-tryptophan, L-tyrosine, and L-phenylalanine, share the same precursors, to increase the titer of a specific aromatic amino acid ...
DOI:
10.1007/s10295-020-02262-y

15. The role of Lys2–Cl−–Lys2 salt linkages in oligomeric intermediates of RbsD protein in Escherichia coli

Author:
Zheng, Jing; Zheng, Jie; Ma, Yuanwu; Zuo, Guocai; Feng, Yongjun
Source:
Journal of basic microbiology 2020 v.60 no.2 pp. 185-194
ISSN:
0233-111X
Subject:
Escherichia coli,   etc   ; crosslinking; dissociation; mutation; protein subunits; proteins;     Show all 6 Subjects
Abstract:
... As a homo‐oligomeric protein, the disassembly of Escherichia coli RbsD decamer produces a urea‐unfolded oligomeric intermediate structure, as the dissociation speed of the protein is lower than that of the unfolding process. There are five Lys2–Cl⁻–Lys2 salt linkages to connect these subunits. To explore the role of the salt linkages in these oligomeric intermediates, the Lys2Ala mutated in the N‐ ...
DOI:
10.1002/jobm.201900337

16. The shortcomings of accurate rate estimations in cultivation processes and a solution for precise and robust process modeling

Author:
Bayer, B.; Sissolak, B.; Duerkop, M.; von Stosch, M.; Striedner, G.
Source:
Bioprocess and biosystems engineering 2020 v.43 no.2 pp. 169-178
ISSN:
1615-7591
Subject:
Escherichia coli,   etc   ; batch fermentation; bioprocessing; cell growth; models;     Show all 5 Subjects
Abstract:
... The accurate estimation of cell growth or the substrate consumption rate is crucial for the understanding of the current state of a bioprocess. Rates unveil the actual cell status, making them valuable for quality-by-design concepts. However, in bioprocesses, the real rates are commonly not accessible due to analytical errors. We simulated Escherichia coli fed-batch fermentations, sampled at four ...
DOI:
10.1007/s00449-019-02214-6
PubMed:
31541314
PubMed Central:
PMC6960212

17. Tuning chromosomal gene expression in Escherichia coli by combining single-stranded oligonucleotides mediated recombination and kil counter selection system

Author:
Chen, Wei; Chen, Ruyi; Wang, Hui; Li, Yujuan; Zhang, Yunyi; Li, Shanhu
Source:
Journal of biotechnology 2020 v.307 pp. 63-68
ISSN:
0168-1656
Subject:
Escherichia coli,   etc   ; gene expression; genes; metabolic engineering; oligonucleotides; transcription (genetics);     Show all 6 Subjects
Abstract:
... Extensively modulating gene expression to achieve optimal flux is a critical step in metabolic engineering. Gene expression is usually modulated at the transcriptional level by controlling the strength of a promoter. However, this type of modulation is often hampered by its inability to fully sample the complete continuum of transcriptional control. In Escherichia coli, this limitation can be solv ...
DOI:
10.1016/j.jbiotec.2019.10.014

18. Characterisation of a cointegrate plasmid harbouring blaNDM-1 in a clinical Salmonella Lomita strain

Author:
Li, Ruichao; Xie, Miaomiao; Liu, Lizhang; Huang, Yonglu; Wu, Xiaoyan; Wang, Zhiqiang; Chan, Edward Wai Chi; Chen, Sheng
Source:
International journal of antimicrobial agents 2020 v.55 no.1 pp. 105817
ISSN:
0924-8579
Subject:
Escherichia coli,   etc   ; Salmonella; anti-infective agents; evolution; plasmids;     Show all 5 Subjects
Abstract:
... This study aimed to characterise the molecular events underlying formation and evolution of a cointegrate plasmid harbouring blaNDM₋₁ and blaCMY₋₂ in a clinical Salmonella Lomita (S. Lomita) strain. The Salmonella strain SL131 was found to harbour two multidrug resistant (MDR) plasmids. One plasmid, pSL131_IncHI2, is a typical IncHI2 plasmid containing blaOXA₋₁, catB3, arr-3, sul1, qnrB4 and blaDH ...
DOI:
10.1016/j.ijantimicag.2019.09.021

19. Diversity of Bacterial Chemosensory Arrays

Author:
Yang, Wen; Briegel, Ariane
Source:
Trends in microbiology 2020 v.28 no.1 pp. 68-80
ISSN:
0966-842X
Subject:
Escherichia coli,   etc   ; bacteria; chemotaxis; models; protein composition; signal transduction;     Show all 6 Subjects
Abstract:
... Chemotaxis is crucial for the survival of bacteria, and the signaling systems associated with it exhibit a high level of evolutionary conservation. The architecture of the chemosensory array and the signal transduction mechanisms have been extensively studied in Escherichia coli. More recent studies have revealed a vast diversity of the chemosensory system among bacteria. Unlike E. coli, some bact ...
DOI:
10.1016/j.tim.2019.08.002

20. E. coli recovery from antimicrobial hand towels used in rural households in Kenya

Author:
Kim, Sunkyung; Quick, Robert; Stauber, Christine; Oremo, Jared; Murphy, Jennifer
Source:
Journal of microbiological methods 2020 v.168 pp. 105776
ISSN:
0167-7012
Subject:
Escherichia coli,   etc   ; drying; field experimentation; hands; households; Kenya;     Show all 6 Subjects
Abstract:
... An antimicrobial towel designed for repeated use was developed to prevent recontamination of washed hands after drying. This field trial in Kenya found that nearly all antimicrobial hand towels and untreated control towels were contaminated with E. coli after household use. The antimicrobial towel did not inactivate E. coli. ...
DOI:
10.1016/j.mimet.2019.105776