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1. Fe(iii)-complex mediated bacterial cell surface immobilization of eGFP and enzymes

Author:
Feng, Lilin; Gao, Liang; Sauer, Daniel F.; Ji, Yu; Cui, Haiyang; Schwaneberg, Ulrich
Source:
Chemical communications 2021 v.57 no.36 pp. 4460-4463
ISSN:
1364-548X
Subject:
Escherichia coli,   etc   ; bacteria; hydrogels;     Show all 3 Subjects
Abstract:
... We report a facile and reversible method to immobilize a broad range of His₆-tagged proteins on the E. coli cell surface through Fe(iii)–metal complexes. A His₆-tagged eGFP and four His₆-tagged enzymes were successfully immobilized on the cell surface. Additionally, a hydrogel sheath around E. coli cells was generated by immobilized His₆-tagged HRP. ...
DOI:
10.1039/d1cc01575c

2. Transient non-specific DNA binding dominates the target search of bacterial DNA-binding proteins

Author:
Stracy, Mathew; Schweizer, Jakob; Sherratt, David J.; Kapanidis, Achillefs N.; Uphoff, Stephan; Lesterlin, Christian
Source:
Molecular cell 2021 v.81 no.7 pp. 1499-1514.e6
ISSN:
1097-2765
Subject:
Escherichia coli,   etc   ; DNA; chromosomes;     Show all 3 Subjects
Abstract:
... Despite their diverse biochemical characteristics and functions, all DNA-binding proteins share the ability to accurately locate their target sites among the vast excess of non-target DNA. Toward identifying universal mechanisms of the target search, we used single-molecule tracking of 11 diverse DNA-binding proteins in living Escherichia coli. The mobility of these proteins during the target sear ...
DOI:
10.1016/j.molcel.2021.01.039

3. More than propellers: how flagella shape bacterial motility behaviors

Author:
Grognot, Marianne; Taute, Katja M
Source:
Current opinion in microbiology 2021 v.61 pp. 73-81
ISSN:
1369-5274
Subject:
Escherichia coli,   etc   ; bacterial motility; flagellum;     Show all 3 Subjects
Abstract:
... Bacteria use a wide variety of flagellar architectures to navigate their environment. While the iconic run-tumble motility strategy of the peritrichously flagellated Escherichia coli has been well studied, recent work has revealed a variety of new motility behaviors that can be achieved with different flagellar architectures, such as single, bundled, or opposing polar flagella. The recent discover ...
DOI:
10.1016/j.mib.2021.02.005

4. Macromolecular aging: ATP hydrolysis-driven functional and structural changes in Escherichia coli RecQ helicase

Author:
Li, Dan; Xu, Hou-Qiang; Xi, Xu-Guang
Source:
Biochemical and biophysical research communications 2021 v.542 pp. 29-33
ISSN:
0006-291X
Subject:
Escherichia coli,   etc   ; age; motors; research;     Show all 4 Subjects
Abstract:
... Aging has been considered a phenomenon that can be only applied to cells or organisms. Here, we show that RecQ helicase from E. coli displays an aging phenomenon: this macromolecular motor loses its structure and function after hydrolyzing a certain number of ATP molecules. The aging process was only triggered by repeated catalytic cycles. These observations lead to a new concept: macromolecule ag ...
DOI:
10.1016/j.bbrc.2021.01.020

5. Retardation of Folding Rates of Substrate Proteins in the Nanocage of GroEL

Author:
Koculi, Eda; Thirumalai, D.
Source:
Biochemistry 2021 v.60 no.6 pp. 460-464
ISSN:
1520-4995
Subject:
Escherichia coli,   etc   ; chaperonins; thiosulfate sulfurtransferase;     Show all 3 Subjects
Abstract:
... The Escherichia coli ATP-consuming chaperonin machinery, a complex between GroEL and GroES, has evolved to facilitate folding of substrate proteins (SPs) that cannot do so spontaneously. A series of kinetic experiments show that the SPs are encapsulated in the GroEL/ES nanocage for a short duration. If confinement of the SPs is the mechanism by which GroEL/ES facilitates folding, it follows that t ...
DOI:
10.1021/acs.biochem.0c00903

6. Conjugation-regulating synthesis of high photosensitizing activity porphyrin-based covalent organic frameworks for photodynamic inactivation of bacteria

Author:
Meng, Fan-Lin; Qian, Hai-Long; Yan, Xiu-Ping
Source:
Talanta 2021 v.233 pp. 122536
ISSN:
0039-9140
Subject:
Escherichia coli,   etc   ; Staphylococcus aureus; human health; porphyrins;     Show all 4 Subjects
Abstract:
... Preparation of porphyrin-based covalent organic frameworks (Por-COFs) with high photosensitizing activity for photodynamic inactivation of bacteria is of great challenge, but significant for economy and human health. Herein, we show a conjugation-regulating strategy to design and synthesize Por-COFs with high photosensitizing activity for the photodynamic inactivation of bacteria. Terephthalaldehy ...
DOI:
10.1016/j.talanta.2021.122536

7. Genome editing by miniature CRISPR/Cas12f1 enzyme in Escherichia coli

Author:
Okano, Kenji; Sato, Yu; Hizume, Tatsuya; Honda, Kohsuke
Source:
Journal of bioscience and bioengineering 2021 v.132 no.2 pp. 120-124
ISSN:
1389-1723
Subject:
Escherichia coli,   etc   ; Streptococcus pyogenes; cytotoxicity; enzymes;     Show all 4 Subjects
Abstract:
... The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system is a valuable genome editing tool for microorganisms. However, the commonly used Cas9 nuclease derived from Streptococcus pyogenes (SpCas9) is not applicable to many industrially relevant bacteria, due to its cytotoxicity and large size (1368 amino acids [aa]). We developed an alternative genome ed ...
DOI:
10.1016/j.jbiosc.2021.04.009

8. Deciphering the effects of CeO2 nanoparticles on Escherichia coli in the presence of ferrous and sulfide ions: Physicochemical transformation-induced toxicity and detoxification mechanisms

Author:
You, Guoxiang; Xu, Yi; Wang, Peifang; Wang, Chao; Chen, Juan; Hou, Jun; Miao, Lingzhan; Gao, Yang; Li, Yan
Source:
Journal of hazardous materials 2021 v.413 pp. 125300
ISSN:
0304-3894
Subject:
Escherichia coli,   etc   ; cytotoxicity; direct contact; sulfides;     Show all 4 Subjects
Abstract:
... The physicochemical transformations as well as the redox reaction-induced toxicity changes of ceria nanoparticles (CeO₂ NPs) in reducing conditions is extremely lacking. Herein, the behaviors, chemical modifications and toxicity of CeO₂ NPs in the presence of reduction-active ions (namely Fe²⁺ and S²⁻) were investigated, with a particular emphasis on the cytotoxicity mechanism associated with thei ...
DOI:
10.1016/j.jhazmat.2021.125300

9. Antimicrobial effectiveness of beverage containers made of silver doped PET

Author:
Braun, Sabrina; Ilberg, Vladimir; Langowski, Horst-Christian
Source:
Journal of food engineering 2021 v.300 pp. 110526
ISSN:
0260-8774
Subject:
Escherichia coli,   etc   ; glass; iced tea; phosphates; silver;     Show all 5 Subjects
Abstract:
... Active packaging based on a release of silver ions (Ag⁺) has become a focus of increasing interest. However, applications are rare for beverages in particular, and the question remains whether the amount for antimicrobial effectiveness is below the migration limit of 0.05 mg Ag/kg food proposed by the EFSA. Experiments were performed using E. coli (10⁶ cfu/mL) in distilled water and Z. bailii (<10 ...
DOI:
10.1016/j.jfoodeng.2021.110526

10. Minimized backbone and novel microaerobic promoters boost plasmid DNA production

Author:
Velazquez, Daniela; Jaén, Karim E.; Sigala, Juan-Carlos; Lara, Alvaro R.
Source:
Process biochemistry 2021 v.106 pp. 130-136
ISSN:
1359-5113
Subject:
Escherichia coli,   etc   ; Vitreoscilla; biomass; hemoglobin; plasmids;     Show all 5 Subjects
Abstract:
... A novel microaerobically inducible pUC18-derived plasmid was produced in Escherichia coli chromosomally expressing Vitreoscilla hemoglobin. The plasmid contains a copy of the positive replication control molecule rnaII under the control of the Pᵥgb promoter. The plasmid was unstable in the engineered strain. Sequencing showed that the plasmid was fragmented during preculture development, probably ...
DOI:
10.1016/j.procbio.2021.04.013

11. AES and ToF-SIMS combination for single cell chemical imaging of gold nanoparticle-labeled Escherichia coli

Author:
Courrèges, Cécile; Bonnecaze, Mélanie; Flahaut, Delphine; Nolivos, Sophie; Grimaud, Régis; Allouche, Joachim
Source:
Chemical communications 2021 v.57 no.44 pp. 5446-5449
ISSN:
1364-548X
Subject:
Escherichia coli,   etc   ; gelatin; gold; mass spectrometry; nanogold;     Show all 5 Subjects
Abstract:
... A chemical fingerprint of the Escherichia coli cell surface labeled by gelatin coated gold nanoparticles was obtained by combining Auger Electron Spectroscopy (AES) for single cell level chemical images, and Time-of-Flight Secondary Ion Mass Spectroscopy (ToF-SIMS) Tandem MS for unambiguous molecular identification of co-localized species. ...
DOI:
10.1039/d1cc01211h

12. Lipoproteins in Gram-negative bacteria: new insights into their biogenesis, subcellular targeting and functional roles

Author:
El Rayes, Jessica; Rodríguez-Alonso, Raquel; Collet, Jean-François
Source:
Current opinion in microbiology 2021 v.61 pp. 25-34
ISSN:
1369-5274
Subject:
Escherichia coli,   etc   ; antibiotics; biogenesis; lipoproteins; moieties;     Show all 5 Subjects
Abstract:
... Bacterial lipoproteins are globular proteins anchored to a membrane by a lipid moiety. By discovering new functions carried out by lipoproteins, recent research has highlighted the crucial roles played by these proteins in the cell envelope of Gram-negative bacteria. Here, after discussing the wide range of activities carried out by lipoproteins in the model bacterium Escherichia coli, we review n ...
DOI:
10.1016/j.mib.2021.02.003

13. TtOmp85, a single Omp85 member protein functions as a β-barrel protein insertase and an autotransporter translocase without any accessory proteins

Author:
Chu, Yindi; Wang, Zhe; Weigold, Sebastian; Norrell, Derrick; Fan, Enguo
Source:
Biochemical and biophysical research communications 2021 v.552 pp. 73-77
ISSN:
0006-291X
Subject:
Escherichia coli,   etc   ; Thermus thermophilus; biogenesis; research;     Show all 4 Subjects
Abstract:
... The biogenesis of outer membrane proteins requires the function of β-barrel assembly machinery (BAM), whose function is highly conserved while its composition is variable. The Escherichia coli BAM is composed of five subunits, while Thermus thermophilus seems to contain a single BAM protein, named TtOmp85. To search for the primitive form of a functional BAM, we investigated and compared the funct ...
DOI:
10.1016/j.bbrc.2021.03.043

14. Drift dynamics in microbial communities and the effective community size

Author:
Sloan, William T.; Nnaji, Chioma F.; Lunn, Mary; Curtis, Thomas P.; Colloms, Sean D.; Couto, Jillian M.; Pinto, Ameet J.; Connelly, Stephanie; Rosser, Susan J.
Source:
Environmental microbiology 2021 v.23 no.5 pp. 2473-2483
ISSN:
1462-2912
Subject:
Escherichia coli,   etc   ; death; immigration; microbiology; population dynamics;     Show all 5 Subjects
Abstract:
... The structure and diversity of all open microbial communities are shaped by individual births, deaths, speciation and immigration events; the precise timings of these events are unknowable and unpredictable. This randomness is manifest as ecological drift in the population dynamics, the importance of which has been a source of debate for decades. There are theoretical reasons to suppose that drift ...
DOI:
10.1111/1462-2920.15453

15. High-level production of l-homoserine using a non-induced, non-auxotrophic Escherichia coli chassis through metabolic engineering

Author:
Zhang, Yu; Wei, Minhua; Zhao, Guihong; Zhang, Wenjie; Li, Yingzi; Lin, Beibei; Li, Yanjun; Xu, Qingyang; Chen, Ning; Zhang, Chenglin
Source:
Bioresource technology 2021 v.327 pp. 124814
ISSN:
0960-8524
Subject:
Escherichia coli,   etc   ; acetates; amino acids; fermentation;     Show all 4 Subjects
Abstract:
... l-Homoserine is a valuable non-proteinogenic amino acid used in the synthesis of various important compounds. Microbial fermentation has potential value for producing l-homoserine on a large scale, but suffers from a low yield and the need for expensive additives. In this study, a non-induced, non-auxotrophic, plasmid-free Escherichia coli chassis for the high-efficiency production of l-homoserine ...
DOI:
10.1016/j.biortech.2021.124814

16. Particle fractionation controls Escherichia coli release from solid manure

Author:
Sepehrnia, Nasrollah; Tabatabaei, Sayyed-Hassan; Norouzi, Hamdollah; Gorakifard, Mohsen; Shirani, Hossein; Rezanezhad, Fereidoun
Source:
Heliyon 2021 v.7 no.5 pp. e07038
ISSN:
2405-8440
Subject:
Escherichia coli,   etc   ; data collection; fractionation; pollution; soil;     Show all 5 Subjects
Abstract:
... Bacteria transport through soil is a complex process particularly when the cells are released from solid manures and co-transported with particles. This study focuses on understanding of the Escherichia coli release from different particle fractions (0.25-, 0.5-, 1-, and 2-mm) of solid manure and evaluating different influent boundary conditions during cell release from manure and when a solid man ...
DOI:
10.1016/j.heliyon.2021.e07038

17. BtsT/ BtsS is involved in glyoxylate transport in E. coli and its mutations facilitated glyoxylate utilization

Author:
Zhao, Tongxing; Xu, Liru; Zhao, Lei; Zhang, Hongjie; Li, Yin; Zhang, Yanping
Source:
Biochemical and biophysical research communications 2021 v.551 pp. 71-77
ISSN:
0006-291X
Subject:
Escherichia coli,   etc   ; genomic libraries; pyruvic acid; research;     Show all 4 Subjects
Abstract:
... Glyoxylate is an important chemical and is also an intermediate involved in metabolic pathways of living microorganisms. However, it cannot be rapidly utilized by many microbes. We observed a very long lag phase (up to 120 h) when E. coli is growing in a mineral medium supplemented with 50 mM glyoxylate. To better understand this strange growth pattern on glyoxylate and accelerate glyoxylate utili ...
DOI:
10.1016/j.bbrc.2021.03.002

18. Recombinant expression of HIV-1 protease using soluble fusion tags in Escherichia coli: A vital tool for functional characterization of HIV-1 protease

Author:
Eche, Simeon; Gordon, Michelle L.
Source:
Virus research 2021 v.295 pp. 198289
ISSN:
0168-1702
Subject:
Escherichia coli,   etc   ; cytotoxicity; proteinases; research; solubility; viruses;     Show all 6 Subjects
Abstract:
... HIV-1 protease expression in the laboratory is demanding because of its high cytotoxicity, making it difficult to express in bacterial expression systems such as Escherichia coli. To overcome these challenges, HIV-1 protease fusion with solubility enhancing tags helps to mitigate its cytotoxic effect and drive its expression as a soluble protein. Therefore, this review focuses on the expression of ...
DOI:
10.1016/j.virusres.2020.198289

19. Genetic Code Expansion: Inception, Development, Commercialization

Author:
Manandhar, Miglena; Chun, Eugene; Romesberg, Floyd E.
Source:
Journal of the American Chemical Society 2021 v.143 no.13 pp. 4859-4878
ISSN:
1520-5126
Subject:
Escherichia coli,   etc   ; commercialization; genetic code; therapeutics;     Show all 4 Subjects
Abstract:
... Virtually all natural proteins are built from only 20 amino acids, and while this makes possible all the functions they perform, the ability to encode other amino acids selected for specific purposes promises to enable the discovery and production of proteins with novel functions, including therapeutic proteins with more optimal drug-like properties. The field of genetic code expansion (GCE) has f ...
DOI:
10.1021/jacs.0c11938

20. Recombinant expression of computationally designed peptide-bundlemers in Escherichia coli

Author:
Sinha, Nairiti J.; Kloxin, Christopher J.; Saven, Jeffery G.; Jensen, Grethe V.; Kelman, Zvi; Pochan, Darrin J.
Source:
Journal of biotechnology 2021 v.330 pp. 57-60
ISSN:
0168-1656
Subject:
Escherichia coli,   etc   ; biocompatible materials; biomimetics; peptides;     Show all 4 Subjects
Abstract:
... Computational design of fully artificial peptides is extensively researched by material scientists and engineers for the construction of novel nanostructures and biomaterials. Such design has yielded a peptide-based building block or bundlemer, a coiled coil peptide assembly that undergoes further physical-covalent interactions to form 1D, 2D and, potentially, 3D hierarchical assemblies and displa ...
DOI:
10.1016/j.jbiotec.2021.03.004