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1. Transient non-specific DNA binding dominates the target search of bacterial DNA-binding proteins

Author:
Stracy, Mathew; Schweizer, Jakob; Sherratt, David J.; Kapanidis, Achillefs N.; Uphoff, Stephan; Lesterlin, Christian
Source:
Molecular cell 2021 v.81 no.7 pp. 1499-1514.e6
ISSN:
1097-2765
Subject:
Escherichia coli,   etc   ; DNA; chromosomes;     Show all 3 Subjects
Abstract:
... Despite their diverse biochemical characteristics and functions, all DNA-binding proteins share the ability to accurately locate their target sites among the vast excess of non-target DNA. Toward identifying universal mechanisms of the target search, we used single-molecule tracking of 11 diverse DNA-binding proteins in living Escherichia coli. The mobility of these proteins during the target sear ...
DOI:
10.1016/j.molcel.2021.01.039

2. More than propellers: how flagella shape bacterial motility behaviors

Author:
Grognot, Marianne; Taute, Katja M
Source:
Current opinion in microbiology 2021 v.61 pp. 73-81
ISSN:
1369-5274
Subject:
Escherichia coli,   etc   ; bacterial motility; flagellum;     Show all 3 Subjects
Abstract:
... Bacteria use a wide variety of flagellar architectures to navigate their environment. While the iconic run-tumble motility strategy of the peritrichously flagellated Escherichia coli has been well studied, recent work has revealed a variety of new motility behaviors that can be achieved with different flagellar architectures, such as single, bundled, or opposing polar flagella. The recent discover ...
DOI:
10.1016/j.mib.2021.02.005

3. Deciphering the effects of CeO2 nanoparticles on Escherichia coli in the presence of ferrous and sulfide ions: Physicochemical transformation-induced toxicity and detoxification mechanisms

Author:
You, Guoxiang; Xu, Yi; Wang, Peifang; Wang, Chao; Chen, Juan; Hou, Jun; Miao, Lingzhan; Gao, Yang; Li, Yan
Source:
Journal of hazardous materials 2021 v.413 pp. 125300
ISSN:
0304-3894
Subject:
Escherichia coli,   etc   ; cytotoxicity; direct contact; sulfides;     Show all 4 Subjects
Abstract:
... The physicochemical transformations as well as the redox reaction-induced toxicity changes of ceria nanoparticles (CeO₂ NPs) in reducing conditions is extremely lacking. Herein, the behaviors, chemical modifications and toxicity of CeO₂ NPs in the presence of reduction-active ions (namely Fe²⁺ and S²⁻) were investigated, with a particular emphasis on the cytotoxicity mechanism associated with thei ...
DOI:
10.1016/j.jhazmat.2021.125300

4. Antimicrobial effectiveness of beverage containers made of silver doped PET

Author:
Braun, Sabrina; Ilberg, Vladimir; Langowski, Horst-Christian
Source:
Journal of food engineering 2021 v.300 pp. 110526
ISSN:
0260-8774
Subject:
Escherichia coli,   etc   ; glass; iced tea; phosphates; silver;     Show all 5 Subjects
Abstract:
... Active packaging based on a release of silver ions (Ag⁺) has become a focus of increasing interest. However, applications are rare for beverages in particular, and the question remains whether the amount for antimicrobial effectiveness is below the migration limit of 0.05 mg Ag/kg food proposed by the EFSA. Experiments were performed using E. coli (10⁶ cfu/mL) in distilled water and Z. bailii (<10 ...
DOI:
10.1016/j.jfoodeng.2021.110526

5. AES and ToF-SIMS combination for single cell chemical imaging of gold nanoparticle-labeled Escherichia coli

Author:
Courrèges, Cécile; Bonnecaze, Mélanie; Flahaut, Delphine; Nolivos, Sophie; Grimaud, Régis; Allouche, Joachim
Source:
Chemical communications 2021 v.57 no.44 pp. 5446-5449
ISSN:
1364-548X
Subject:
Escherichia coli,   etc   ; gelatin; gold; mass spectrometry; nanogold;     Show all 5 Subjects
Abstract:
... A chemical fingerprint of the Escherichia coli cell surface labeled by gelatin coated gold nanoparticles was obtained by combining Auger Electron Spectroscopy (AES) for single cell level chemical images, and Time-of-Flight Secondary Ion Mass Spectroscopy (ToF-SIMS) Tandem MS for unambiguous molecular identification of co-localized species. ...
DOI:
10.1039/d1cc01211h

6. Lipoproteins in Gram-negative bacteria: new insights into their biogenesis, subcellular targeting and functional roles

Author:
El Rayes, Jessica; Rodríguez-Alonso, Raquel; Collet, Jean-François
Source:
Current opinion in microbiology 2021 v.61 pp. 25-34
ISSN:
1369-5274
Subject:
Escherichia coli,   etc   ; antibiotics; biogenesis; lipoproteins; moieties;     Show all 5 Subjects
Abstract:
... Bacterial lipoproteins are globular proteins anchored to a membrane by a lipid moiety. By discovering new functions carried out by lipoproteins, recent research has highlighted the crucial roles played by these proteins in the cell envelope of Gram-negative bacteria. Here, after discussing the wide range of activities carried out by lipoproteins in the model bacterium Escherichia coli, we review n ...
DOI:
10.1016/j.mib.2021.02.003

7. TtOmp85, a single Omp85 member protein functions as a β-barrel protein insertase and an autotransporter translocase without any accessory proteins

Author:
Chu, Yindi; Wang, Zhe; Weigold, Sebastian; Norrell, Derrick; Fan, Enguo
Source:
Biochemical and biophysical research communications 2021 v.552 pp. 73-77
ISSN:
0006-291X
Subject:
Escherichia coli,   etc   ; Thermus thermophilus; biogenesis; research;     Show all 4 Subjects
Abstract:
... The biogenesis of outer membrane proteins requires the function of β-barrel assembly machinery (BAM), whose function is highly conserved while its composition is variable. The Escherichia coli BAM is composed of five subunits, while Thermus thermophilus seems to contain a single BAM protein, named TtOmp85. To search for the primitive form of a functional BAM, we investigated and compared the funct ...
DOI:
10.1016/j.bbrc.2021.03.043

8. High-level production of l-homoserine using a non-induced, non-auxotrophic Escherichia coli chassis through metabolic engineering

Author:
Zhang, Yu; Wei, Minhua; Zhao, Guihong; Zhang, Wenjie; Li, Yingzi; Lin, Beibei; Li, Yanjun; Xu, Qingyang; Chen, Ning; Zhang, Chenglin
Source:
Bioresource technology 2021 v.327 pp. 124814
ISSN:
0960-8524
Subject:
Escherichia coli,   etc   ; acetates; amino acids; fermentation;     Show all 4 Subjects
Abstract:
... l-Homoserine is a valuable non-proteinogenic amino acid used in the synthesis of various important compounds. Microbial fermentation has potential value for producing l-homoserine on a large scale, but suffers from a low yield and the need for expensive additives. In this study, a non-induced, non-auxotrophic, plasmid-free Escherichia coli chassis for the high-efficiency production of l-homoserine ...
DOI:
10.1016/j.biortech.2021.124814

9. BtsT/ BtsS is involved in glyoxylate transport in E. coli and its mutations facilitated glyoxylate utilization

Author:
Zhao, Tongxing; Xu, Liru; Zhao, Lei; Zhang, Hongjie; Li, Yin; Zhang, Yanping
Source:
Biochemical and biophysical research communications 2021 v.551 pp. 71-77
ISSN:
0006-291X
Subject:
Escherichia coli,   etc   ; genomic libraries; pyruvic acid; research;     Show all 4 Subjects
Abstract:
... Glyoxylate is an important chemical and is also an intermediate involved in metabolic pathways of living microorganisms. However, it cannot be rapidly utilized by many microbes. We observed a very long lag phase (up to 120 h) when E. coli is growing in a mineral medium supplemented with 50 mM glyoxylate. To better understand this strange growth pattern on glyoxylate and accelerate glyoxylate utili ...
DOI:
10.1016/j.bbrc.2021.03.002

10. Genetic Code Expansion: Inception, Development, Commercialization

Author:
Manandhar, Miglena; Chun, Eugene; Romesberg, Floyd E.
Source:
Journal of the American Chemical Society 2021 v.143 no.13 pp. 4859-4878
ISSN:
1520-5126
Subject:
Escherichia coli,   etc   ; commercialization; genetic code; therapeutics;     Show all 4 Subjects
Abstract:
... Virtually all natural proteins are built from only 20 amino acids, and while this makes possible all the functions they perform, the ability to encode other amino acids selected for specific purposes promises to enable the discovery and production of proteins with novel functions, including therapeutic proteins with more optimal drug-like properties. The field of genetic code expansion (GCE) has f ...
DOI:
10.1021/jacs.0c11938

11. Recombinant expression of computationally designed peptide-bundlemers in Escherichia coli

Author:
Sinha, Nairiti J.; Kloxin, Christopher J.; Saven, Jeffery G.; Jensen, Grethe V.; Kelman, Zvi; Pochan, Darrin J.
Source:
Journal of biotechnology 2021 v.330 pp. 57-60
ISSN:
0168-1656
Subject:
Escherichia coli,   etc   ; biocompatible materials; biomimetics; peptides;     Show all 4 Subjects
Abstract:
... Computational design of fully artificial peptides is extensively researched by material scientists and engineers for the construction of novel nanostructures and biomaterials. Such design has yielded a peptide-based building block or bundlemer, a coiled coil peptide assembly that undergoes further physical-covalent interactions to form 1D, 2D and, potentially, 3D hierarchical assemblies and displa ...
DOI:
10.1016/j.jbiotec.2021.03.004

12. Imaging-Based Optofluidic Biolaser Array Encapsulated with Dynamic Living Organisms

Author:
Gong, Xuerui; Feng, Shilun; Qiao, Zhen; Chen, Yu-Cheng
Source:
Analytical chemistry 2021 v.93 no.14 pp. 5823-5830
ISSN:
1520-6882
Subject:
Escherichia coli,   etc   ; analytical chemistry; antibiotics; fluorescence;     Show all 4 Subjects
Abstract:
... Optofluidic biolasers have emerged as promising tools for biomedical analysis due to their strong light–matter interactions and miniaturized size. Recent developments in optofluidic lasers have opened a new Frontier in monitoring biological processes. However, most biolasers require precise recording of the lasing spectrum at the single cavity level, which limits its application in high-throughput ...
DOI:
10.1021/acs.analchem.1c00020

13. Food Safety and E. coli in Aquaponic and Hydroponic Systems

Author:
Sawyer, Tawnya
Source:
Horticulturae 2021 v.7 no.3
ISSN:
2311-7524
Subject:
Escherichia coli,   etc   ; aquaponics; food safety; hydroponics;     Show all 4 Subjects
Abstract:
... The following document is The Aquaponic Association’s response to a recent publication on E. coli in Aquaponic and Hydroponic systems [...] ...
DOI:
10.3390/horticulturae7030036

14. Reply to Comment on “The Occurrence of Shiga Toxin-Producing E. coli in Aquaponic and Hydroponic Systems”

Author:
Wang, Yi-Ju; Deering, Amanda J.; Kim, Hye-Ji
Source:
Horticulturae 2021 v.7 no.3
ISSN:
2311-7524
Subject:
Shiga toxin-producing Escherichia coli,   etc   ; aquaponics; hydroponics;     Show all 3 Subjects
Abstract:
... Recently, the Aquaponic Association (AA) published a statement through multiple outlets in response to our article entitled “The Occurrence of Shiga Toxin-Producing E. coli in Aquaponic and Hydroponic Systems” [...] ...
DOI:
10.3390/horticulturae7030037

15. Assessing changes in the expression levels of cell surface proteins with a turn-on fluorescent molecular probe

Author:
Hatai, Joydev; Prasad, Pragati Kishore; Lahav-Mankovski, Naama; Oppenheimer-Low, Noa; Unger, Tamar; Sirkis, Yael Fridmann; Dadosh, Tali; Motiei, Leila; Margulies, David
Source:
Chemical communications 2021 v.57 no.15 pp. 1875-1878
ISSN:
1364-548X
Subject:
Escherichia coli,   etc   ; fluorescence; outer membrane proteins;     Show all 3 Subjects
Abstract:
... Tri-nitrilotriacetic acid (NTA)-based fluorescent probes were developed and used to image His-tagged-labelled outer membrane protein C (His-OmpC) in live Escherichia coli. One of these probes was designed to light up upon binding, which provided the means to assess changes in the His-OmpC expression levels by taking a simple fluorescence spectrum. ...
DOI:
10.1039/d0cc07095e

16. Genetically encoding ε-N-benzoyllysine in proteins

Author:
Ji, Yanli; Ren, Conghui; Miao, Hui; Pang, Zhili; Xiao, Ruotong; Yang, Xiaochen; Xuan, Weimin
Source:
Chemical communications 2021 v.57 no.14 pp. 1798-1801
ISSN:
1364-548X
Subject:
Escherichia coli,   etc   ; ; histones; lysine; mammals; sirtuins;     Show all 6 Subjects
Abstract:
... Lysine ε-N-benzoylation is a recently identified PTM occurring on histone proteins, and herein we genetically encoded ε-N-benzoyl-lysine (BzK) into recombinant proteins in E. coli and mammalian cells, and applied it for the modification of histone proteins and the analysis of sirtuin debenzoylase activity. ...
DOI:
10.1039/d0cc07954e

17. Primer reporte de Escherichia coli diarreogénica en población pediátrica ambulatoria con diarrea atendida en la ciudad de La Plata, Argentina

Author:
Molina, Nora Beatriz; Oderiz, Sebastián; Vescina, Cecilia; Córdoba, Alejandra; Basualdo, Juan Ángel; Sparo, Mónica Delfina
Source:
Revista argentina microbiología 2021
ISSN:
0325-7541
Subject:
Shiga toxin-producing Escherichia coli; enteroinvasive Escherichia coli; enteropathogenic Escherichia coli,   etc   ; Shiga-like toxin 2; diarrhea; enterotoxigenic Escherichia coli; feces; hospitals; pathotypes; virulence; Argentina;     Show all 11 Subjects
Abstract:
... Diarrheagenic Escherichia coli is a heterogeneous group of strains that presents various virulence factors and causes different diarrheal syndromes. The most studied pathotypes are enteropathogenic Escherichia coli (EPEC), enterotoxigenic Escherichia coli (ETEC), enteroaggregative Escherichia coli (EAEC), enteroinvasive Escherichia coli (EIEC) and Shiga toxin-producing Escherichia coli (STEC). The ...
DOI:
10.1016/j.ram.2021.02.006

18. Genetic characterization of Shigatoxigenic and enteropathogenic Escherichia coli O80:H2 from diarrhoeic and septicaemic calves and relatedness to human Shigatoxigenic E. coli O80:H2

Author:
Habets, A.; Crombé, F.; Nakamura, K.; Guérin, V.; De Rauw, K.; Piérard, D.; Saulmont, M.; Hayashi, T.; Mainil, J.G.; Thiry, D.
Source:
Journal of applied microbiology 2021 v.130 no.1 pp. 258-264
ISSN:
1364-5072
Subject:
enterohemorrhagic Escherichia coli; enteropathogenic Escherichia coli,   etc   ; feces; genes; humans; intestines; kidneys; microbiology; phylogeny; serotypes; Belgium;     Show all 11 Subjects
Abstract:
... AIM: The purpose of this work was to identify and genetically characterize enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) O80:H2 from diarrhoeic and septicaemic calves in Belgium and to comparing them with human EHEC after whole genome sequencing. METHODS AND RESULTS: Ten EHEC and 21 EPEC O80 identified by PCR between 2009 and 2018 from faeces, intestinal content and ...
DOI:
10.1111/jam.14759

19. Syntheses of the Carotane-type Terpenoids (+)-Schisanwilsonene A and (+)-Tormesol via a Two-Stage Approach

Author:
Mou, Shu-Bin; Xiao, Wen; Wang, Hua-Qi; Chen, Kai-Yue; Xiang, Zheng
Source:
Organic letters 2020 v.23 no.2 pp. 400-404
ISSN:
1523-7052
Subject:
Escherichia coli,   etc   ; stereoselectivity; terpenoids;     Show all 3 Subjects
Abstract:
... Stereoselective syntheses of terpenoids in a more efficient manner have been a long-term pursuit for synthetic chemists. Herein we describe the two-step, enantiospecific and protecting-group-free synthesis of (+)-schisanwilsonene A from a carotane compound, which was produced in E. coli. We also completed the first enantiomeric synthesis of (+)-tormesol in five steps. The two-stage strategy offers ...
DOI:
10.1021/acs.orglett.0c03894

20. Frequency modulation of the Min-protein oscillator by nucleoid-associated factors in Escherichia coli

Author:
Shen, Jie-Pan; Chang, Yi-Ren; Chou, Chia-Fu
Source:
Biochemical and biophysical research communications 2020 v.525 no.4 pp. 857-862
ISSN:
0006-291X
Subject:
Escherichia coli,   etc   ; geometry; research;     Show all 3 Subjects
Abstract:
... In E. coli, the Min-protein oscillator, together with the nucleoid occlusion (NO), destabilizes the Z-ring assembly away from the midcell to ensure faithful septation. These two inhibitory pathways are thought to be working independently for division site placement. Even though the Min-protein oscillator has been displayed by synthetic minimal systems, it is unclear the interplays of Min proteins ...
DOI:
10.1016/j.bbrc.2020.02.161