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EscherichiacoliO157, etc ; genetic variation; monitoring; phylogeny; single nucleotide polymorphism; temperature; Show all 6 Subjects
Abstract:
... Single-nucleotide polymorphisms (SNPs) are one of the most common forms of genetic variation and as such are powerful tools for the identification of bacterial strains, their genetic diversity, phylogenetic analysis, and outbreak surveillance. In this study, we used 15 sets of SNP-containing primers to amplify and sequence the target Escherichia coli. Based on the combination of the 15-sequence pr ...
EscherichiacoliO157, etc ; Pseudomonas; biofilm; food microbiology; meat; risk; spoilage; virulence; Show all 8 Subjects
Abstract:
... Escherichia coli O157:H7 and Pseudomonas were considered as common colonizers of fresh and spoilage meat, where they tended to live in the proximity. In this study, we tested the interplay between different isolates of E. coli O157:H7 and Pseudomonas in random two-by-two combinations grown as dual-species consortia. Results showed that the growth fitness of E. coli was not facilitated by the prese ...
EscherichiacoliO157, etc ; analytical chemistry; electrochemistry; food pathogens; human health; public safety; Show all 6 Subjects
Abstract:
... As a notorious foodborne pathogen, Escherichia coli O157:H7 (E. coli O157:H7) poses a major threat to human health, public safety and economic property all around the globe, early detection is essential. In recent years, a series of novel detection strategies for the rapid, specific and sensitive detection of E. coli O157:H7 have been developed, but a systematic and comprehensive overview of purel ...
EscherichiacoliO157, etc ; Naegleria fowleri; encephalitis; glucose; mannose; parasitology; pathogenicity; proteinases; research; Show all 9 Subjects
Abstract:
... Naegleria fowleri can cause acute primary amoebic encephalitis. It is known that contact-dependent pathogenicity in free-living amoeba may be mediated through a carbohydrate-dependent pathway. In this study, the effect of mannose on the interaction between N. fowleri and pathogenic Escherichia coli O157:H7 and non-pathogenic E. coli DH5α was analyzed. In particular, the changes in proteases expres ...
EscherichiacoliO157, etc ; Salmonella; Shigella flexneri; flow cytometry; ground beef; rapid methods; Show all 6 Subjects
Abstract:
... Salmonella, Escherichia coli O157, and Shigella flexneri are typical foodborne pathogens in ground beef, which can cause severe infection even when present as a single cell. Flow cytometry (FCM) methods are widely applied in the rapid detection of pathogens in food products. In this study, we report an FCM-based method for detecting single cells of Salmonella, E. coli O157, and S. flexneri in 25 g ...
EscherichiacoliO157, etc ; biosensors; chemiluminescence; food pathogens; morbidity; mortality; nucleic acids; rapid methods; Show all 8 Subjects
Abstract:
... E. coli O157:H7 has become a crucial foodborne pathogen with certain morbidity and mortality. However, the available methods still face the significant challenges including the improvement of detection specificity and sensitivity for real sample analysis. Herein, a microfluidic chemiluminescence biosensor based on a multiple signal amplification strategy was developed for rapid and ultrasensitive ...
EscherichiacoliO157, etc ; colorimetry; detection limit; food safety; irradiation; public health; temperature; thermometers; Show all 8 Subjects
Abstract:
... Rapid and portable detection of foodborne pathogens is of great significance for food safety and public health. The colorimetric methods based on naked-eye have been demonstrated to be a suitable qualitative method for point-of-care testing (POCT). However, analytical instruments like a microplate reader must be needed for the quantitative assay. To overcome its limitation, we herein report a nove ...
EscherichiacoliO157, etc ; Legionella; Salmonella; biosensors; detection limit; dielectrophoresis; electrodes; pathogens; tap water; wastewater; Show all 10 Subjects
Abstract:
... A microfluidic based biosensor was investigated for rapid and simultaneous detection of Salmonella, Legionella, and Escherichia coli O157:H7 in tap water and wastewater. The biosensor consisted of two sets of focusing electrodes connected in parallel and three sets of interdigitated electrodes (IDE) arrays. The electrodes enabled the biosensor to concentrate and detect bacteria at both low and hig ...
EscherichiacoliO157, etc ; Bacillus cereus; RNA; bacteria; cell death; food irradiation; pepper; pollution; virulence; Show all 9 Subjects
Abstract:
... Although irradiation has been used to destroy bacteria and molds in spices globally, a new technology is needed to alleviate public fears of irradiation, the high cost of irradiated food, and environmental contamination at the irradiation facility. The objective of this study was to investigate the effects of dielectric barrier discharge (DBD) plasma on Eshcericah coli O157: H7 and Bacillus cereus ...
EscherichiacoliO157, etc ; bacterial colonization; food science; irrigation; laminar flow; lettuce; microfluidic technology; porosity; spinach; Show all 9 Subjects
Abstract:
... This study investigated the persistence of Escherichia coli O157:H7 on the surface of leafy greens, and penetration into intercellular spaces through stomatal pores. This was done by determining the role of leafy green-mimicking microgrooves (normal and fully open stomata and micro-sized crevices) and the roles of pore size and orientation on proliferation of E. coli. Microgroove-embedded microflu ...
EscherichiacoliO157, etc ; Listeria monocytogenes; Salmonella; breasts; chickens; flow cytometry; food contamination; quantitative analysis; rapid methods; Show all 9 Subjects
Abstract:
... Chicken breast is prone to Listeria monocytogenes, Salmonella spp., and E. coli O157:H7 contaminations. Therefore, a quantitative method for the rapid detection of these pathogens simultaneously in chicken breast is highly desirable for food safety control. This study reports a flow cytometry (FCM)-based method for rapid and multiplexed quantification of the above-stated three pathogens without en ...
EscherichiacoliO157, etc ; antibacterial properties; beef; chitosan; fibroins; hydrolysis; nanofibers; sensory properties; serine proteinases; tensile strength; Show all 10 Subjects
Abstract:
... Cinnamaldehyde (CA) possesses high antibacterial activity, but it's unstable and volatile in food system. To overcome its drawbacks, a kind of dual-response controlled-release nanofiber was fabricated by electrospinning to embed CA@hyaluronic acid/carboxymethyl chitosan nanoparticles (CA@HA/CMCS-NPs) into silk fibroin nanofibers (CA@HA/CMCS-NPs SF nanofibers). When the concentration of CA@HA/CMCS- ...
EscherichiacoliO157, etc ; anti-infective agents; bacteriophages; beef; chlorites; food quality; serotypes; trade; ultraviolet radiation; vacuum packaging; Show all 10 Subjects
Abstract:
... A high event period (HEP) occurs when beef processing facilities experience an elevated rate of STEC positive trim samples. In order to avoid contaminated vacuum-packaged beef entering into commerce, primals and subprimals associated to positive trim must be treated with antimicrobials, repackaged, and retested for STEC to ensure product wholesomeness. In this study, we evaluated the efficacy of B ...
... Shiga toxin producing Escherichia coli (STEC) are common etiological agents of food borne illnesses and outbreaks, most often caused by consuming contaminated beef products, followed by raw vegetables and dairy products. Patients infected with E. coli O157 are more likely hospitalized than patients infected with non-O157 STEC, making E. coli O157 an important target for microbiological interventio ...
EscherichiacoliO157, etc ; chemical species; chemiluminescence; detection limit; enzyme immunoassays; food safety; nanocomposites; peroxidase; storage quality; tap water; Show all 10 Subjects
Abstract:
... Immunoassays based on enzyme-labeled antibodies have been widely used in the food safety field. However, the production process of enzyme-labeled antibodies is complicated and the low storage stability limits their application. Herein, antibody–horseradish peroxidase (HRP) co-assembled nanocomposites (AHC NCs) with outstanding advantages such as enhanced stability, lower cost, and substrate affini ...
... Strawberries can be contaminated by foodborne pathogens or mold on farm or during postharvest handling. Photo-irradiation of edible chitosan coating containing gallic acid can be an innovative antimicrobial intervention to improve the safety and shelf-life of strawberries. This study investigated antimicrobial activity of chitosan-gallic acid coating under 360 nm UV-A light. The thin layer of coat ...
... This study aimed to develop a combination of immunomagnetic separation (IMS) and real-time PCR (qPCR) techniques to detect and analyze Salmonella spp. (SAL), Listeria monocytogenes (LM), and Escherichia coli O157:H7 (O157: H7) in meat samples quickly and accurately. Bacteria-specific immunomagnetic beads (IMBs) were prepared from carboxyl magnetic beads and affinity-purified polyclonal antibodies ...
... The objectives of the present study were: (i) to record time-temperature data in the geometrical center (“coldest spots”) of different sizes and states (raw vs. frozen) of pork “gyros” during realistic cooking scenarios, (ii) to model the growth of Salmonella spp., Escherichia coli O157:H7, Staphylococcus aureus, Clostridium sporogenes (as a surrogate of Clostridiumbotulinum), and lactic acid bact ...
... The sensitive detection of foodborne pathogens is of great significance for ensuring food safety and quality. Herein, on the basis of methylene blue-embedded zirconium based organic framework (UIO@MB) as the remarkable capture carrier and signal indicator, with the Al³⁺-assisted the fluorescent signal response, we developed a label-free and dual-signal lateral flow immunochromatographic assay (LDL ...