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1. Ultrabright fluorescent microsphere and its novel application for improving the sensitivity of immunochromatographic assay

Author:
Zhang, Gang-gang; Xu, Shao-lan; Xiong, Yong-hua; Duan, Hong; Chen, Wen-yao; Li, Xiang-min; Yuan, Mei-fang; Lai, Wei-hua
Source:
Biosensors & bioelectronics 2019 v.135 pp. 173-180
ISSN:
0956-5663
Subject:
Escherichia coli O157,   etc   ; biosensors; detection limit; fluorescence; immunoaffinity chromatography; microparticles;     Show all 6 Subjects
Abstract:
... Fluorescent microsphere (FM) is widely used as probe in immunochromatographic assay (ICA). However, the performance of conventional FM is limited because of the aggregation-caused quenching effect. Herein, we compared a kind of conventional FM (DMFFM, loading DMF) with novel aggregation-induced emission FM (AIEFM, loading TCBPE). The fluorescence intensity of DMFFM initially increased and then dec ...
DOI:
10.1016/j.bios.2019.04.023

2. Novel ELISA based on fluorescent quenching of DNA-stabilized silver nanoclusters for detecting E. coli O157:H7

Author:
Wang, Chun; Xing, Keyu; Zhang, Ganggang; Yuan, Meifang; Xu, Shaolan; Liu, Daofeng; Chen, Wenyao; Peng, Juan; Hu, Song; Lai, Wei-Hua
Source:
Food chemistry 2019 v.281 pp. 91-96
ISSN:
0308-8146
Subject:
Escherichia coli O157,   etc   ; enzyme-linked immunosorbent assay; fluorescence; human health; nanogold; nanosilver; single-stranded DNA;     Show all 7 Subjects
Abstract:
... Escherichia coli O157:H7 (E. coli O157:H7) is a potential threat to human health; thus, a rapid and sensitive method for detecting it is necessary. We designed a single-stranded DNA that contained an appended block and anchoring block. The appended block acted as a scaffold to prepare fluorescent Ag nanoclusters (AgNCs). The anchoring block contained Poly A, which bound with the surface of gold na ...
DOI:
10.1016/j.foodchem.2018.12.079

3. Survival and internalization of Salmonella and Escherichia coli O157:H7 sprayed onto different cabbage cultivars during cultivation in growth chambers

Author:
Erickson, Marilyn C; Liao, Jye‐Yin; Payton, Alison S; Cook, Peter W; Ortega, Ynes R
Source:
Journal of the science of food and agriculture 2019 v.99 no.7 pp. 3530-3537
ISSN:
0022-5142
Subject:
Escherichia coli O157,   etc   ; Salmonella; cabbage; cultivars; enteropathogens; growth chambers; maturity stage; risk reduction;     Show all 8 Subjects
Abstract:
... BACKGROUND: Cabbage may become contaminated with enteric pathogens during cultivation. Using multiple cabbage cultivars at two maturity stages (small plants or plants with small heads) in growth chamber studies, the fate (internalization or surface survival) of Salmonella and Escherichia coli O157:H7 (0157) were examined in conjunction with any potential relationships to the plant's antimicrobial ...
DOI:
10.1002/jsfa.9573

4. Recovery from mild Escherichia coli O157:H7 infection in young and aged C57BL/6 mice with intact flora estimated by fecal shedding, locomotor activity and grip strength

Author:
Malavolta, Marco; Basso, Andrea; Giacconi, Robertina; Orlando, Fiorenza; Pierpaoli, Elisa; Cardelli, Maurizio; Leoni, Francesca; Chierichetti, Serena; Bray, Dorothy; Benlhassan, Khadija; Provinciali, Mauro
Source:
Comparative immunology, microbiology, and infectious diseases 2019 v.63 pp. 1-9
ISSN:
0147-9571
Subject:
Escherichia coli O157,   etc   ; at-risk population; discriminant analysis; flora; food pathogens; locomotion; mice; models; monitoring;     Show all 9 Subjects
Abstract:
... Escherichia coli 0157:H7 is a food-borne pathogen that can cause severe complications in vulnerable populations. Mouse infection models of E. coli 0157:H7 are usually developed under severe animal suffering classification by depleting the normal flora, in which age plays a role.To develop a refined method for longitudinal monitoring of E. coli 0157:H7 in young and old mice with intact flora.We app ...
DOI:
10.1016/j.cimid.2018.12.003

5. A novel method based on fluorescent magnetic nanobeads for rapid detection of Escherichia coli O157:H7

Author:
Huang, Zhen; Peng, Juan; Han, Jiaojiao; Zhang, Ganggang; Huang, Youju; Duan, Miaolin; Liu, Daofeng; Xiong, Yonghua; Xia, Shiqi; Lai, Weihua
Source:
Food chemistry 2019 v.276 pp. 333-341
ISSN:
0308-8146
Subject:
Escherichia coli O157,   etc   ; fluorescence; food pathogens; immunoassays; immunomagnetic separation; magnetism; monoclonal antibodies; nanoparticles; photoluminescence; rapid methods;     Show all 10 Subjects
Abstract:
... Fluorescent lateral flow immunoassay (FLFIA) based on immunomagnetic separation (IMS) has the advantage of sensitivity. However, its complex operation includes IMS, elution, incubation, and FLFIA steps. Here, we prepared a core@shell@satellite structure fluorescent magnetic nanobeads (FMNBs) and firstly introduced them into the novel method that integrated IMS with FLFIA (I-IMS-FLFIA) for the dete ...
DOI:
10.1016/j.foodchem.2018.09.164

6. Butia odorata Barb. Rodr. extract inhibiting the growth of Escherichia coli in sliced mozzarella cheese

Author:
Maia, Darla Silveira Volcan; Haubert, Louise; dos Santos Soares, Kauana; de Fátima Rauber Würfel, Simone; da Silva, Wladimir Padilha
Source:
Journal of food science and technology 2019 v.56 no.3 pp. 1663-1668
ISSN:
0022-1155
Subject:
Escherichia coli O157,   etc   ; Butia odorata; antimicrobial properties; genes; mozzarella cheese; palmitic acid; phenotype; virulence; Brazil;     Show all 9 Subjects
Abstract:
... The aims of this study were to verify the occurrence of Escherichia coli in sliced mozzarella cheese marketed in Pelotas city, Brazil and perform the phenotypic and genotypic characterization of the isolates. Besides that, evaluate the susceptibility of E. coli to Butia odorata extract, characterize it chemically, and apply the extract in sliced mozzarella cheese contaminated experimentally with E ...
DOI:
10.1007/s13197-019-03686-w

7. Resistance of various shiga-toxin producing Escherichia coli (STEC) strains and serogroups to infra-red and pulsed UV radiation and effect of nalidixic acid adaptation

Author:
Chintagari, Sailaja; Jadeja, Raviraj; Hung, Yen-Con
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2019 v.102 pp. 356-363
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; Escherichia coli O104; Escherichia coli O26; acid tolerance; nalidixic acid; serotypes; ultraviolet radiation;     Show all 7 Subjects
Abstract:
... The efficacy of Infra-red radiation (IR) and Pulsed UV (PUV) for the inactivation of parent (Par) and Nalidixic acid adapted (Nal) strains of Escherichia coli O157:H7, top six shiga-toxin producing E. coli (E. coli O26, E. coli O45, E. coli O103, E. coli O111, E. coli O121, and E. coli O145 and E. coli O104) along with nonpathogenic surrogates were evaluated. It was observed that significant (P <  ...
DOI:
10.1016/j.lwt.2018.11.094

8. Safety risks associated with dispersal of E. coli O157:H7 in home sprouting modules

Author:
Mulaosmanovic, Emina; Farkas, Sebastian; Vågsholm, Ivar; Darlison, Julia; Sousa, Maria; Mogren, Lars; Gharaie, Samareh; Alsanius, Beatrix W.
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2019 v.101 pp. 783-788
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; animal pathogens; bacteria; fenugreek; lifestyle; markets; models; organic foods; risk; seeds; sprouting; Scandinavia;     Show all 12 Subjects
Abstract:
... Despite numerous outbreaks of foodborne illnesses associated with sprouts, consumption of raw sprouted seeds is still linked with a healthy lifestyle in Scandinavia. Different types of sprouting modules for home sprout production are available on the market, and sold without information about risks associated with sprouting. During sprouting, conditions are ideal for seed emergence, but also for b ...
DOI:
10.1016/j.lwt.2018.11.086

9. Plasmonic enhancement in lateral flow sensors for improved sensing of E. coli O157:H7

Author:
Ren, Wen; Ballou, Dexter R.; FitzGerald, Ryan; Irudayaraj, Joseph
Source:
Biosensors & bioelectronics 2019 v.126 pp. 324-331
ISSN:
0956-5663
Subject:
Escherichia coli O157,   etc   ; biosensors; colorimetry; encapsulation; immunoassays; liquids; microbial detection; nanogold; polyethyleneimine; rapid methods; translation (genetics);     Show all 11 Subjects
Abstract:
... We propose a plasmonic enhanced lateral flow sensor (pLFS) concept with an enhanced colorimetric signal by utilizing liposome encapsulating reagent to trigger the aggregation of gold nanoparticles (GNPs). Our signal enhancement strategy incorporates the simplicity of lateral flow immunoassays (LFIA) utilizing plasmonic enhancement. The conceptualized hybrid pLFS for onsite rapid detection of patho ...
DOI:
10.1016/j.bios.2018.10.066

10. Internalization assessment of E. coli O157:H7 in hydroponically grown lettuce

Author:
Moriarty, Matthew J.; Semmens, Kenneth; Bissonnette, Gary K.; Jaczynski, Jacek
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2019 v.100 pp. 183-188
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; aquaponics; cutting; decontamination; hydroponics; lettuce; models; root systems; roots;     Show all 9 Subjects
Abstract:
... Hydroponics and aquaponics have been gaining popularity to grow produce. Escherichia coli O157:H7 may internalize within edible produce. This study was to investigate E. coli O157:H7 internalization in red sails lettuce in a custom designed/constructed model hydroponic system under laboratory-controlled conditions. The objective was to determine not only the likelihood and level of internalization ...
DOI:
10.1016/j.lwt.2018.10.060

11. Validation on milk and sprouts of EN ISO 16654:2001 - Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157

Author:
Tozzoli, Rosangela; Maugliani, Antonella; Michelacci, Valeria; Minelli, Fabio; Caprioli, Alfredo; Morabito, Stefano
Source:
International journal of food microbiology 2019 v.288 pp. 53-57
ISSN:
0168-1605
Subject:
Escherichia coli O157,   etc   ; European Union; food analysis; food chain; foods; microbiology; milk;     Show all 7 Subjects
Abstract:
... In 2006, the European Committee for standardisation (CEN)/Technical Committee 275 - Food analysis - Horizontal methods/Working Group 6 - Microbiology of the food chain (TC275/WG6), launched the project of validating the method ISO 16654:2001 for the detection of Escherichia coli O157 in foodstuff by the evaluation of its performance, in terms of sensitivity and specificity, through collaborative s ...
DOI:
10.1016/j.ijfoodmicro.2018.05.005

12. Novel Continuous and Manual Sampling Methods for Beef Trim Microbiological Testing

Author:
Wheeler, Tommy L.; Arthur, Terrance, M.
Source:
Journal of food protection 2018 v.81 no.10 pp. 1605-1613
ISSN:
0362-028X
Subject:
Escherichia coli O157,   etc   ; Salmonella; beef; indicator species; industry; pathogens; sampling;     Show all 7 Subjects
Abstract:
... A sampling method that represents a greater proportion of the beef trimmings in a 900-kg combo bin should improve the current pathogen sampling and detection programs used by fresh beef processors. This study compared two novel, nondestructive sampling methodologies (a continuous sampling device [CSD] and a manual sampling device [MSD]) with the current industry methodologies, the N60 Excision (th ...
Handle:
10113/6199940
DOI:
10.4315/0362-028X.JFP-18-197

13. Combination effect of saturated or superheated steam and lactic acid on the inactivation of Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytogenes on cantaloupe surfaces

Author:
Kwon, Sun-Ah; Song, Won-Jae; Kang, Dong-Hyun
Source:
Food microbiology 2019 v.82 pp. 342-348
ISSN:
0740-0020
Subject:
Escherichia coli O157,   etc   ; Listeria monocytogenes; Salmonella Typhimurium; acid treatment; cantaloupes; color; detection limit; food pathogens; lactic acid; spraying; steam; texture;     Show all 12 Subjects
Abstract:
... The purpose of this study was to evaluate the effectiveness of the combination treatment of lactic acid immersion and saturated or superheated steam (SHS) on inactivation of foodborne pathogens on cantaloupes. Saturated steam (SS) treatments were performed at 100 °C, while SHS treatments were delivered at either 150 or 200 °C. Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytog ...
DOI:
10.1016/j.fm.2019.03.012

14. Curli fimbriae confer shiga toxin-producing Escherichia coli a competitive trait in mixed biofilms

Author:
Carter, Michelle Qiu; Feng, Doris; Li, Hui Hong
Source:
Food microbiology 2019 v.82 pp. 482-488
ISSN:
0740-0020
Subject:
Escherichia coli O157,   etc   ; biofilm; biogenesis; fimbriae; food pathogens; genes; leaves; microorganisms; mutants; phenotype; spinach; stainless steel; temperature;     Show all 13 Subjects
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) is one of the most common causal agents of foodborne illness linked to fresh leafy vegetables. Here, we investigated the impact of spinach-associated microorganisms on proliferation and biofilm formation of STEC O157:H7 on stainless steel surfaces at temperatures related to produce production and postharvest processing environments. Although a prolifer ...
DOI:
10.1016/j.fm.2019.03.024

15. Effect of 222-nm krypton-chloride excilamp treatment on inactivation of Escherichia coli O157:H7 and Salmonella Typhimurium on alfalfa seeds and seed germination

Author:
Kang, Jun-Won; Lee, Jae-Ik; Jeong, Soo-Yeon; Kim, Yu-Min; Kang, Dong-Hyun
Source:
Food microbiology 2019 v.82 pp. 171-176
ISSN:
0740-0020
Subject:
Escherichia coli O157,   etc   ; DNA damage; Salmonella Typhimurium; alfalfa; food pathogens; mercury; seed germination; seed industry; seed treatment; seeds;     Show all 10 Subjects
Abstract:
... We examined the control effect of a 222-nm KrCl excilamp on foodborne pathogens on alfalfa seeds and compared it with a conventional 254-nm low-pressure (LP) Hg lamp. When the 222-nm KrCl excilamp treated seeds at 87, 174 and 261 mJ/cm2, the log reductions of Escherichia coli O157:H7 (E. coli O157:H7) were 0.85, 1.77, and 2.77, respectively, and Salmonella Typhimurium (S. Typhimurium) experienced ...
DOI:
10.1016/j.fm.2019.01.019

16. UV-C irradiated gallic acid exhibits enhanced antimicrobial activity via generation of reactive oxidative species and quinone

Author:
Wang, Qingyang; Leong, Wai Fun; Elias, Ryan J.; Tikekar, Rohan V.
Source:
Food chemistry 2019 v.287 pp. 303-312
ISSN:
0308-8146
Subject:
Escherichia coli O157,   etc   ; antimicrobial properties; gallic acid; hydrogen peroxide; hydrogen production; irradiation; oxidative stress; pH; quinones; ultraviolet radiation; wavelengths;     Show all 11 Subjects
Abstract:
... A novel light-enhanced antimicrobial treatment was developed by exposing gallic acid (GA) to UV-C light. GA (15 mM) solution was exposed to UV-C for 30 min and subsequently incubated with E. coli O157:H7 for 30 min to achieve a 3.2 ± 0.2 log CFU/mL reduction. The antimicrobial activity is affected by the irradiation duration, wavelength, and pH of solution. The addition of benzenesulfinic acid (BS ...
DOI:
10.1016/j.foodchem.2019.02.041

17. Decontamination of bovine hide surfaces for enhancing food safety: Use of alkyltrimethylammonium bromide and chlorhexidine digluconate

Author:
Long, Wilbert; Sarker, Majher I.; Annous, Bassam A.; Paoli, George C.
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2019 v.109 pp. 255-260
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; Listeria monocytogenes; Salmonella enterica; beef; chlorhexidine; decontamination; food safety; hides and skins; pathogens; quaternary ammonium compounds;     Show all 10 Subjects
Abstract:
... This study evaluated the efficacy of alkyltrimethylammonium-bromide (ATMB) and chlorhexidine-digluconate (CDG) to reduce pathogens of meat safety concerns in solution and on hides. In solution, ATMB at 50 and 250 ppm reduced individual cocktails of Salmonella enterica, Escherichia coli O157:H7 and Listeria monocytogenes at averages of 1.77 and 3.76, 1.11 and 5.78, 0.52 and 2.55 log CFU/mL respecti ...
DOI:
10.1016/j.lwt.2019.04.005

18. Hydrodynamic cavitation for the rapid separation and electrochemical detection of Cryptosporidium parvum and Escherichia coli O157:H7 in ground beef

Author:
Nze, Ugochukwu C.; Beeman, Michael G.; Lambert, Christopher J.; Salih, Ghadhanfer; Gale, Bruce K.; Sant, Himanshu J.
Source:
Biosensors & bioelectronics 2019 v.135 pp. 137-144
ISSN:
0956-5663
Subject:
Escherichia coli O157,   etc   ; Cryptosporidium parvum; biosensors; diarrhea; electrochemistry; food matrix; food pathogens; ground beef; hydrodynamics; immunomagnetic separation; industry; oocysts;     Show all 12 Subjects
Abstract:
... Foodborne illnesses are a major contributor to misery and health challenges in both rich and poor nations. Illnesses from pathogens such as Escherichia coli and Cryptosporidium parvum oocysts account for most of the cases of diarrhea in the world. Many standard methods exist for detecting these pathogens in water. However, these standard methods do not readily translate to the detection of the sam ...
DOI:
10.1016/j.bios.2019.04.002

19. Naked eye detection of an amplified gene using metal particle-based DNA transport within functionalized porous interfaces

Author:
Park, Yoo Min; Kim, Chi Hyun; Song, Younseong; Hwang, Sang-Hyun; Lee, Seok Jae; Lee, Moon-Keun
Source:
Talanta 2019 v.195 pp. 97-102
ISSN:
0039-9140
Subject:
Escherichia coli O157,   etc   ; DNA; agarose; color; gels; genes; heavy metals; molecular weight; point-of-care testing; polymerase chain reaction;     Show all 10 Subjects
Abstract:
... The current study focuses on developing a system for visually detecting an amplified bacterial (Escherichia coli O157:H7) gene using a heavy metal particle (MP) and functionalized porous sepharose gel. To functionalize DNA-specificity to the MP, an avidin-modified MP was employed in combination with a biotin-conjugated primer. The porous sepharose matrix was functionalized with an amine-reactive g ...
DOI:
10.1016/j.talanta.2018.11.037

20. Inactivation effect of UV-C and mild heat treatment against Salmonella Typhimurium and Escherichia coli O157:H7 on black pepper powder

Author:
Park, Mi-Jung; Kim, Jin-Hee; Oh, Se-Wook
Source:
Food science and biotechnology 2019 v.28 no.2 pp. 599-607
ISSN:
1226-7708
Subject:
Escherichia coli O157,   etc   ; Salmonella Typhimurium; black pepper; color; heat treatment; pathogens; physicochemical properties; ultraviolet radiation; water content;     Show all 9 Subjects
Abstract:
... In this study, UV-C and mild heat treatments were used alone or in combination for the inactivation of Salmonella Typhimurium and Escherichia coli O157:H7 on the black pepper powder. The treated pepper powder was analyzed microbiologically, and the physicochemical properties of the pepper powder were examined. UV-C combined with heat treatment at 60 °C most effectively inactivated the pathogens. H ...
DOI:
10.1007/s10068-018-0480-4