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1. Changes in STEC and bacterial communities during enrichment of manufacturing beef in selective and non-selective media

Author:
Kang, Sanga; Ravensdale, Joshua T.; Coorey, Ranil; Dykes, Gary A.; Barlow, Robert S.
Source:
Food microbiology 2021 v.96 pp. 103711
ISSN:
0740-0020
Subject:
Escherichia coli O157,   etc   ; Clostridium; beef; food microbiology; peptones; slaughterhouses;     Show all 6 Subjects
Abstract:
... Detection and isolation of Shiga toxin-producing Escherichia coli (STEC) from manufacturing beef is challenging and it may be affected by microbial changes during enrichment. This study was designed to understand population changes during enrichment of beef from an integrated (Samples A and B) and a fragmented (Samples C and D) abattoir. The samples were enriched in buffered peptone water (BPW), A ...
DOI:
10.1016/j.fm.2020.103711

2. Synthesis and Bioactivities of Derivatives of the Diterpenoid Isosteviol with 1,2,3,4-Tetrazole-5-Thiol Moiety

Author:
Liu, C. J.; Wang, Y. F.; Yao, J. H.; Ke, M.; Zhai, X. F.; Wan, Q. H.
Source:
Chemistry of natural compounds 2021 v.57 no.1 pp. 88-90
ISSN:
0009-3130
Subject:
Escherichia coli O157,   etc   ; Bacillus subtilis; Staphylococcus aureus; diterpenoids; moieties;     Show all 5 Subjects
Abstract:
... A novel 1,2,3,4-tetrazole-5-thiol-fused isosteviol derivative 3 has been synthesized and structurally characterized by IR, NMR, and HR-MS. It is more sensitive to Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis than Gram-negative bacteria E. coli O157:H7. The results of anticancer activities demonstrated that compound 3 exhibited better anticancer activities against HCT-116 cell ...
DOI:
10.1007/s10600-021-03289-y

3. Effects of High Hydrostatic Pressure and Beef Patty Formulations on the Inactivation of Native Strains of Shiga Toxin-Producing Escherichia coli O157:H7

Author:
Eccoña Sota, Amparo; Cap, Mariana; Rodriguez, Anabel; Szerman, Natalia; Speroni, Francisco; Vaudagna, Sergio R.
Source:
Food and bioprocess technology 2021 v.14 no.6 pp. 1194-1198
ISSN:
1935-5130
Subject:
Escherichia coli O157,   etc   ; beef; bioprocessing; detection limit; high pressure treatment; lipid content; patties; spoilage microorganisms;     Show all 8 Subjects
Abstract:
... This study aimed to evaluate the effects of beef patty formulations and pressure levels on the inactivation of spoilage microbiota and native Shiga toxin-producing Escherichia coli (STEC) O157:H7. Beef patties were made with different salt and fat content (A: 1% NaCl – 10% fat, B: 2% NaCl – 10% fat, C: 1% NaCl – 20% fat and D: 2% NaCl – 20% fat) and treated at 400 and 600 MPa. STEC reductions afte ...
DOI:
10.1007/s11947-021-02648-5

4. A Novel Real-Time Loop-Mediated Isothermal Amplification Combined with Immunomagnetic Beads Separation and Ethidium Bromide Monoazide Treatment for Rapid and Ultrasensitive Detection of Viable Escherichia coli O157:H7 in Milk

Author:
Chen, Hu; Li, Ya-Ke; Zhang, Tian-Tian; Bi, Yan; Shu, Mei; Zhong, Chan; Tang, Kai-Jie; Wu, Guo-Ping
Source:
Food analytical methods 2021 v.14 no.5 pp. 944-956
ISSN:
1936-9751
Subject:
Escherichia coli O157,   etc   ; DNA; detection limit; ethidium; flagellum; loop-mediated isothermal amplification; milk; pH;     Show all 8 Subjects
Abstract:
... In this study, a real-time loop-mediated isothermal amplification (Rti-LAMP) combined with immunomagnetic beads separation (IMS) and ethidium bromide monoazide (EMA) treatment was developed for the rapid and ultrasensitive detection of viable Escherichia coli (E. coli) O157:H7 in milk without enrichment. Polyclonal antibodies against E. coli O157:H7 flagellum and specific immunomagnetic beads (IMB ...
DOI:
10.1007/s12161-020-01932-y

5. Efficacy of gaseous chlorine dioxide in reducing Salmonella enterica, E. coli O157:H7, and Listeria monocytogenes on strawberries and blueberries

Author:
Luu, Phillip; Chhetri, Vijay Singh; Janes, Marlene E.; King, Joan M.; Adhikari, Achyut
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2021 v.141 pp. 110906
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; Listeria monocytogenes; Salmonella enterica; biological safety cabinet; blueberries; chlorine dioxide; pathogens; sanitation; strawberries;     Show all 9 Subjects
Abstract:
... Gaseous chlorine dioxide, a strong oxidizing agent can be utilized as an effective alternative sanitizing agent. This study evaluated the efficacy of low concentration (≤5 mg/L) gaseous chlorine dioxide (ClO₂) treatment with gas permeable sachets in reducing the levels of Salmonella, E. coli O157:H7, and Listeria monocytogenes on strawberries and blueberries. Strawberries and blueberries were spot ...
DOI:
10.1016/j.lwt.2021.110906

6. An aptamer-exonuclease III (Exo III)–assisted amplification-based lateral flow assay for sensitive detection of Escherichia coli O157:H7 in milk

Author:
Ren, Yuwei; Gao, Pingping; Song, Yang; Yang, Xinyan; Yang, Tao; Chen, Sihan; Fu, Shiqian; Qin, Xue; Shao, Meili; Man, Chaoxin; Jiang, Yujun
Source:
Journal of dairy science 2021
ISSN:
0022-0302
Subject:
Escherichia coli O157,   etc   ; ; dairy science; death; detection limit; enzymes; milk; nanogold; oligonucleotides;     Show all 9 Subjects
Abstract:
... Escherichia coli O157:H7 (E. coli O157:H7), one of the most widespread foodborne pathogens, can cause a series of diseases and even lead to death. In this study, a highly sensitive method was developed by combining aptamer-exonuclease III (Exo III)–assisted amplification with lateral flow assay (LFA) based on gold nanoparticles (AuNP). The compound of single-stranded (ss) DNA-anti-E. coli O157:H7 ...
DOI:
10.3168/jds.2020-19939

7. Effects of direct and in-package pulsed light treatment on inactivation of E. coli O157:H7 and reduction of microbial loads in Romaine lettuce

Author:
Mukhopadhyay, Sudarsan; Sokorai, Kimberly; Ukuku, Dike O.; Jin, Tony; Fan, Xuetong; Olanya, Ocen M.; Leng, Juncai; Juneja, Vijay
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2021 v.139 pp. 110710
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; decontamination; fresh produce; inoculum; pathogens; polyethylene; risk; romaine lettuce; yeasts;     Show all 9 Subjects
Abstract:
... The microbial safety of fresh produce continues to be a real concern. Novel nonthermal technologies are required to lessen the risk of pathogen contamination. The objective of this research was to develop and evaluate the effects of direct and in-package pulsed light (PL) treatment on the survival of E. coli O157:H7 in Romaine lettuce. Treatment influence on reduction of background microbial loads ...
DOI:
10.1016/j.lwt.2020.110710

8. Escherichia coli O157 survival in liquid culture and artificial soil microcosms with variable pH, humic acid and clay content

Author:
Baker, C.A.; De, J.; Schneider, K.R.
Source:
Journal of applied microbiology 2021 v.130 no.2 pp. 416-423
ISSN:
1364-5072
Subject:
Escherichia coli O157,   etc   ; aluminum sulfate; bentonite; clay; clay fraction; humic acids; kaolinite; liquids; microbiology; pH; sand;     Show all 11 Subjects
Abstract:
... AIMS: This research was performed to investigate the influence of clay and humic acid on Escherichia coli O157 survival in model soils. Additionally, the influence of pH and humic acid on E. coli O157 in liquid culture was investigated. METHODS AND RESULTS: Artificial soil microcosms were prepared with sand, kaolinite, bentonite and humic acid. Artificial soil microcosms pH was adjusted (6·0–7·0) ...
DOI:
10.1111/jam.14775

9. Physicomechanical properties, release kinetics, and antimicrobial activity of activated low-density polyethylene and orientated polypropylene films by Thyme essential oil active component

Author:
Mousavian, Dinasadat; Mohammadi Nafchi, Abdorreza; Nouri, Leila; Abedinia, Ahmadreza
Source:
Journal of food measurement & characterization 2021 v.15 no.1 pp. 883-891
ISSN:
2193-4126
Subject:
Escherichia coli O157,   etc   ; Staphylococcus aureus; color; essential oils; measurement; models; polyethylene; polypropylenes; tensile strength; thyme; thymol;     Show all 11 Subjects
Abstract:
... In this research active packaging based on orientated polypropylene (OPP) or low-density polyethylene (LDPE) and thymol were prepared and characterized. OPP and LDPE films were immersed in thymol for 1, 2, and 3 days. The mechanical, color, and microbial properties were examined. Film antibacterial properties were evaluated against Staphylococcus aureus ATCC 6538 bacteria as the Gram-positive mode ...
DOI:
10.1007/s11694-020-00690-z

10. Effect of active essential oils added to chicken tawook on the behaviour of Listeria monocytogenes, Salmonella spp. and Escherichia coli O157:H7 during storage

Author:
Osaili, Tareq M.; Hasan, Fayeza; Dhanasekaran, Dinesh Kumar; Obaid, Reyad S.; Al-Nabulsi, Anas A.; Ayyash, Mutamed; Karam, Layal; Savvaidis, Ioannis N.; Holley, Richard
Source:
International journal of food microbiology 2021 v.337 pp. 108947
ISSN:
0168-1605
Subject:
Escherichia coli O157,   etc   ; Listeria monocytogenes; Salmonella; antimicrobial properties; breasts; carvacrol; chickens; essential oils; food pathogens; thymol;     Show all 10 Subjects
Abstract:
... The objective of the study was to assess the antimicrobial effect of active essential oil components (EOs) namely (carvacrol (CA), cinnamaldehyde (CI) and thymol (TH)) on Listeria monocytogenes, Salmonella spp., and Escherichia coli O157:H7 in chicken tawook during storage at 4 and 10 °C. A marinade consisting of ingredients commonly used in the chicken tawook recipe was prepared and mixed with 1% ...
DOI:
10.1016/j.ijfoodmicro.2020.108947

11. Acid resistance of E. coli O157:H7 and O26:H11 exposure to lactic acid revealed by transcriptomic analysis

Author:
Yu, Lanlin; Zhang, Su; Xu, Yuanyuan; Mi, Xiaoyu; Xing, Tong; Li, Jiaolong; Zhang, Lin; Gao, Feng; Jiang, Yun
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2021 v.136 pp. 110352
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; acid tolerance; acid treatment; biofilm; biosynthesis; cysteine; food contamination; lactic acid; pH; transcriptomics;     Show all 10 Subjects
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) strains are classified as important foodborne pathogens and their acid resistance (AR) capability is identified as one of key factors compromising food safety. In this study, we confirmed experimentally that the growth of two STEC strains (O157:H7 and O26:H11) was remarkably inhibited in Luria-Bertani medium (final pH 3.5) supplemented with lactic acid ...
DOI:
10.1016/j.lwt.2020.110352

12. Inactivation of Salmonella Typhimurium, Escherichia coli O157:H7, Staphylococcus aureus, and Listeria monocytogenes in cardamom using 150 KeV low-energy X-ray

Author:
Zhang, Hongfei; Seck, Hon Luen; Zhou, Weibiao
Source:
Innovative food science & emerging technologies 2021 v.67 pp. 102556
ISSN:
1466-8564
Subject:
Escherichia coli O157,   etc   ; Listeria monocytogenes; Salmonella Typhimurium; Staphylococcus aureus; X-radiation; cardamom; dimethyl disulfide; food irradiation;     Show all 8 Subjects
Abstract:
... This study aimed to investigate the inactivation effect of 150 KeV low-energy X-ray on Salmonella Typhimurium, Escherichia coli O157:H7, Staphylococcus aureus, and Listeria monocytogenes that were inoculated in dry cardamom. The D₁₀ value for E. coli O157:H7 was 71.43 Gy and the tR values for S. Typhimurium, L. monocytogenes, and S. aureus were 53.57, 87.74, and 114.64 Gy, respectively. The irradi ...
DOI:
10.1016/j.ifset.2020.102556

13. Salt, glucose, glycine, and sucrose protect Escherichia coli O157:H7 against acid treatment in laboratory media

Author:
Bae, Young-Min; Song, Hana; Lee, Sun-Young
Source:
Food microbiology 2021 v.100 pp. 103854
ISSN:
0740-0020
Subject:
Escherichia coli O157,   etc   ; acetic acid; acid treatment; food microbiology; glucose; malic acid; propionic acid; sucrose; synergism; water activity;     Show all 10 Subjects
Abstract:
... This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth. Additionally, the effectiveness of combining organic acids and various concentrations of salt (0–18%) or sucrose (0–100%) with different water activity values against E. coli O157:H7 were evaluated. For ...
DOI:
10.1016/j.fm.2021.103854

14. Konjac glucomannan-based edible films loaded with thyme essential oil: Physical properties and antioxidant-antibacterial activities

Author:
Liu, Zhe; Lin, Dehui; Shen, Rui; Zhang, Runguang; Liu, Liu; Yang, Xingbin
Source:
Food packaging and shelf life 2021 v.29 pp. 100700
ISSN:
2214-2894
Subject:
Escherichia coli O157,   etc   ; Amorphophallus; antioxidants; essential oils; hydrophilicity; konjac mannan; microstructure; roughness; shelf life; tensile strength; thyme;     Show all 11 Subjects
Abstract:
... The objectives of this study were to develop the edible films based on konjac glucomannan (KGM) loading with different concentrations of thyme essential oil (TEO) (0–1.6 %, v/v) and investigate their properties. The microstructure analyses showed that TEO was evenly distributed in the films and significantly influenced the roughness of the films. Moreover, the hydrophilic characteristics of KGM fi ...
DOI:
10.1016/j.fpsl.2021.100700

15. Synergistic antimicrobial activity of plasma-activated water and propylparaben: Mechanism and applications for fresh produce sanitation

Author:
Liu, Xiao; Li, Yunfei; Wang, Shaodan; Huangfu, Lulu; Zhang, Mengyan; Xiang, Qisen
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2021 v.146 pp. 111447
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; antibacterial properties; antioxidant activity; cell membranes; chlorophyll; color; fresh produce; membrane potential; reactive oxygen species; sanitation; spinach;     Show all 11 Subjects
Abstract:
... Plasma-activated water (PAW) exhibits synergistic antimicrobial activity with propylparaben (PP), but the underlying mechanisms are not fully understood. Thus, the present study aimed to investigate the synergistic mechanism of the two antimicrobial agents and their application in decontaminating fresh produce. More than 6-log reduction in Escherichia coli O157:H7 cells was achieved after PAW trea ...
DOI:
10.1016/j.lwt.2021.111447

16. Polycaprolactone film functionalized with bacteriophage T4 promotes antibacterial activity of food packaging toward Escherichia coli

Author:
Choi, Inyoung; Yoo, Da Som; Chang, Yoonjee; Kim, So Yeon; Han, Jaejoon
Source:
Food chemistry 2021 v.346 pp. 128883
ISSN:
0308-8146
Subject:
Escherichia coli O157,   etc   ; antibacterial properties; bacteriophages; beef; chemical bonding; food chemistry; food industry; food pathogens; host specificity; polymers; sonication;     Show all 11 Subjects
Abstract:
... Bacteriophages (phages) have been extensively utilized as antibacterial agents in the food industry because of their host-specificity. However, their application in polymer films has been limited because of the lack of a strong attachment method for phage to the surface. We developed an antibacterial film by covalently immobilizing Escherichia coli (E. coli)-specific phage T4 on a polycaprolactone ...
DOI:
10.1016/j.foodchem.2020.128883

17. Evaluation of DNA barcode abiotic surrogate as a predictor for inactivation of E. coli O157:H7 during spinach washing

Author:
Bolten, Samantha; Gu, Ganyu; Gulbronson, Connor; Kramer, Matthew; Luo, Yaguang; Zografos, Antonios; Nou, Xiangwu
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2021 v.145 pp. 111321
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; DNA barcoding; anti-infective agents; chemical species; chlorine; cross contamination; decontamination; regression analysis; risk; spinach;     Show all 10 Subjects
Abstract:
... Fresh-cut leafy greens are washed in water with antimicrobials such as free chlorine to mitigate risk of pathogen cross-contamination. Validation of the process in a commercial setting poses challenges, including the identification of effective surrogates that can be used directly on the processing line during commercial operation. The objective of this study was to evaluate the use of an abiotic, ...
DOI:
10.1016/j.lwt.2021.111321

18. Whole transcriptome sequencing analysis of the effect of high hydrostatic pressure on Escherichia coli O157:H7

Author:
Zhu, Jinyan; Li, Shenmiao; Yan, Tingcai; Sun, Xiyun; Ma, Lidan; Meng, Xianjun; Lu, Xiaonan
Source:
Journal of agriculture and food research 2021 v.4 pp. 100147
ISSN:
2666-1543
Subject:
Escherichia coli O157,   etc   ; agriculture; bacteria; food research; food safety; gene expression regulation; high pressure treatment; sequence analysis; transcriptome;     Show all 9 Subjects
Abstract:
... High hydrostatic pressure (HHP) is a widely accepted nonthermal technology for ensuring food safety without compromising nutritional values and organoleptic properties. This study investigated the survival and molecular responses of Escherichia coli O157:H7 when it was subjected to HHP. The inactivation of E. coli O157:H7 by HHP was observed at 250 MPa and higher pressures (p < 0.05), but not at 5 ...
DOI:
10.1016/j.jafr.2021.100147

19. Effect of inkjet-printed flexible dielectric barrier discharge plasma on reduction of pathogen and quality changes on sliced cheese

Author:
Heo, Ye Seul; Yim, Dong Gyun; Baek, Ki Ho; Kang, Taemin; Lee, Yee Eun; Kim, Jinwoo; Choe, Wonho; Jo, Cheorun
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2021 v.143 pp. 111128
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; Cheddar cheese; Listeria monocytogenes; Salmonella Typhimurium; color; lipid peroxidation; microbiological quality; nitrogen; ozone; pH; pathogens; thiobarbituric acid;     Show all 12 Subjects
Abstract:
... The objective of this study was to investigate pathogen reduction and quality changes in sliced cheddar cheese treated with inkjet-printed flexible dielectric barrier discharge (FXDBD) plasma. The samples were treated with plasma for 0, 5, 10, 15, and 20 min. The surviving populations of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes were significantly reduced by 2.65 ...
DOI:
10.1016/j.lwt.2021.111128

20. Photocatalytic graphitic carbon nitride-chitosan composites for pathogenic biofilm control under visible light irradiation

Author:
Shen, Hongchen; Durkin, David P.; Aiello, Ashlee; Diba, Tara; Lafleur, John; Zara, Jason M.; Shen, Yun; Shuai, Danmeng
Source:
Journal of hazardous materials 2021 v.408 pp. 124890
ISSN:
0304-3894
Subject:
Escherichia coli O157,   etc   ; Pseudomonas aeruginosa; Staphylococcus epidermidis; biofilm; carbon nitride; chitosan; graphene; irradiation; light; oxidation; photocatalysis; photocatalysts; polymers; urine;     Show all 14 Subjects
Abstract:
... Photocatalysis holds promise for inactivating environmental pathogens. Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications. The broad-spectrum biofilm inhibition and eradication of the photocatalytic c ...
DOI:
10.1016/j.jhazmat.2020.124890