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1. Transforming kinetic model into a stochastic inactivation model: Statistical evaluation of stochastic inactivation of individual cells in a bacterial population

Author:
Hiura, Satoko; Abe, Hiroki; Koyama, Kento; Koseki, Shigenobu
Source:
Food microbiology 2020 v.91 pp. 103508
ISSN:
0740-0020
Subject:
Escherichia coli O157,   etc   ; Monte Carlo method; Poisson distribution; bacteria; exposure assessment; kinetics; prediction; probabilistic models; tryptones;     Show all 9 Subjects
Abstract:
... Kinetic models performing point estimation are effective in predicting the bacterial behavior. However, the large variation of bacterial behavior appearing in a small number of cells, i.e. equal or less than 10² cells, cannot be expressed by point estimation. We aimed to predict the variation of Escherichia coli O157:H7 behavior during inactivation in acidified tryptone soy broth (pH3.0) through M ...
DOI:
10.1016/j.fm.2020.103508

2. Gold Nanoparticle-Coated Starch Magnetic Beads for the Separation, Concentration, and SERS-Based Detection of E. coli O157:H7

Author:
You, Sang-Mook; Luo, Ke; Jung, Jong-Yun; Jeong, Ki-Baek; Lee, Eun-Seon; Oh, Mi-Hwa; Kim, Young-Rok
Source:
ACS applied materials & interfaces 2020 v.12 no.16 pp. 18292-18300
ISSN:
1944-8252
Subject:
Escherichia coli O157,   etc   ; Raman spectroscopy; antibodies; bacteria; detection limit; gold; magnetism; nanogold; starch; virulent strains;     Show all 10 Subjects
Abstract:
... Here, we report gold nanoparticle-coated starch magnetic beads (AuNP@SMBs) that were prepared by in situ synthesis of AuNPs on the surface of SMBs. Upon functionalization of the surface with a specific antibody, the immuno-AuNP@SMBs were found to be effective in separating and concentrating the target pathogenic bacteria, Escherichia coli O157:H7, from an aqueous sample as well as providing a hots ...
DOI:
10.1021/acsami.0c00418

3. Efficacy of bacteriophage and organic acids in decreasing STEC O157:H7 populations in beef kept under vacuum and aerobic conditions: A simulated High Event Period scenario

Author:
Shebs, E.L.; Lukov, M.J.; Giotto, F.M.; Torres, E.S.; de Mello, A.S.
Source:
Meat science 2020 v.162 pp. 108023
ISSN:
0309-1740
Subject:
Escherichia coli O157,   etc   ; aerobic conditions; anti-infective agents; bacteriophages; beef; industry; lactic acid; peracetic acid;     Show all 8 Subjects
Abstract:
... After High Event Periods, beef subprimals are usually removed from vacuum and treated with antimicrobials. After re-packaging, subprimals are tested to verify the presence of STEC. In this study, bacteriophage and organic acids were applied on beef contaminated with STEC O157:H7 to evaluate the efficiency of industry practices. Beef samples inoculated with STEC were treated with bacteriophage, lac ...
DOI:
10.1016/j.meatsci.2019.108023

4. Heat resistance in Escherichia coli and its implications on ground beef cooking recommendations in Canada

Author:
Yang, Xianqin; Tran, Frances; Klassen, Mark D.
Source:
Journal of food safety 2020 v.40 no.2 pp. e12769
ISSN:
0149-6085
Subject:
Escherichia coli O157,   etc   ; cooking; culture media; ground beef; heat; heat tolerance; pouches; temperature; Canada;     Show all 9 Subjects
Abstract:
... This study assessed the adequacy of the current cooking recommendations in relation to heat resistant Escherichia coli by evaluating eight potentially heat resistant E. coli strains (four generic and four E. coli O157:H7) along with AW1.7. The D₆₀°C‐values for these strains varied from 1.3 to 9.0 min, with J3 and AW1.7 being the least and most heat resistant strains, respectively. The D₆₀°C‐values ...
DOI:
10.1111/jfs.12769

5. Development of a bacterial DNA extraction modular chip using a magnetic particle and portable vibration motor

Author:
Lim, Sun Young; Lee, Tae Jae; Shin, Seol Yi; Bae, Nam Ho; Lee, Seok Jae; Park, Yoo Min
Source:
Analytical methods 2020 v.12 no.9 pp. 1197-1202
ISSN:
1759-9679
Subject:
Escherichia coli O157,   etc   ; DNA; gel electrophoresis; magnetism; mixing; pathogens; quantitative polymerase chain reaction; vibration;     Show all 8 Subjects
Abstract:
... A novel modular chip-based bacterial DNA extraction device was developed to overcome limitations associated with conventional DNA extraction processes that involve lab-scale instrument systems. The DNA extraction modular chip consists of a core chamber, a magnetic jig, and a vibration jig. The vibration jig allows the precise blending of solutions such as a lysis buffer and target bacterial sample ...
DOI:
10.1039/c9ay02690h

6. Dramatically Enhanced Immunochromatographic Assay Using Cascade Signal Amplification for Ultrasensitive Detection of Escherichia coli O157:H7 in Milk

Author:
Fu, Jinmei; Zhou, Yaofeng; Huang, Xiaolin; Zhang, Wenjing; Wu, Yuhao; Fang, Hao; Zhang, Cunzheng; Xiong, Yonghua
Source:
Journal of agricultural and food chemistry 2020 v.68 no.4 pp. 1118-1125
ISSN:
1520-5118
Subject:
Escherichia coli O157,   etc   ; catalytic activity; cross reaction; detection limit; gold; immunoaffinity chromatography; milk; nanogold; pathogens;     Show all 9 Subjects
Abstract:
... The conventional colloidal gold immunochromatographic assay (AuNP-ICA) cannot meet the requirements for the rapid and sensitive detection of Escherichia coli (E. coli) O157:H7 because of its poor sensitivity. Herein, a novel two-step cascade signal amplification strategy that integrates in situ gold growth and nanozyme-mediated catalytic deposition was proposed to enhance the detection sensitivity ...
DOI:
10.1021/acs.jafc.9b07076

7. Effect of a bacteriophage T5virus on growth of Shiga toxigenic Escherichia coli and Salmonella strains in individual and mixed cultures

Author:
Niu, Yan D.; Liu, Hui; Johnson, Roger P.; McAllister, Tim A.; Stanford, Kim
Source:
Virology journal 2020 v.17 no.1 pp. 3
ISSN:
1743-422X
Subject:
Escherichia coli O157,   etc   ; Salmonella enterica; bacteriophages; biological control; biological control agents; culture media; mixed culture; pathogens; serotypes; toxigenic strains;     Show all 10 Subjects
Abstract:
... A previously isolated a bacteriophage, vB_EcoS_AKFV33 of T5virus, demonstrated great potential in biocontrol of Shiga toxigenic Escherichia coli (STEC) O157. This study further evaluated its potential as a biocontrol agent in broth culture against other important non-O157 serogroups of STEC and Salmonella. AKFV33 was capable of lysing isolates of STEC serogroups O26 (n = 1), O145 (n = 1) and Salmo ...
DOI:
10.1186/s12985-019-1269-7
PubMed:
31910855
PubMed Central:
PMC6947960

8. Individual and combined mild heat and UV-C processes for orange juice against Escherichia coli O157:H7

Author:
Pagal, Gladess A.; Gabriel, Alonzo A.
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2020 v.126 pp. 109295
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; ascorbic acid; color; colorimetry; heat; irradiation; microbiological quality; orange juice; population dynamics; ultraviolet radiation;     Show all 10 Subjects
Abstract:
... This study was conducted to evaluate the individual and combined efficacies of mild heating at 53 °C and ultraviolet-C (UV–C) irradiation at 1.14 mW/cm² (0.0114 kJ/m² s) against E. coli O157:H7 in orange juice. Processes were calculated from inactivation parameters and applied to orange juice either individually such as heating to inactivate 5 log cycles of the organism (H₅D), UV-C irradiation to ...
DOI:
10.1016/j.lwt.2020.109295

9. In‐milk inactivation of Escherichia coli O157:H7 by the environmental lytic bacteriophage ECPS‐6

Author:
Grygorcewicz, Bartłomiej; Chajęcka‐Wierzchowska, Wioleta; Augustyniak, Adrian; Wasak, Agata; Stachurska, Xymena; Nawrotek, Paweł; Dołęgowska, Barbara
Source:
Journal of food safety 2020 v.40 no.2 pp. e12747
ISSN:
0149-6085
Subject:
Escherichia coli O157,   etc   ; bacteriophages; biological control; detection limit; food pathogens; milk; milk contamination; pH; raw milk; refrigeration;     Show all 10 Subjects
Abstract:
... Shiga toxin‐producing Escherichia coli is a common foodborne pathogen which transmission includes dairy products. In the search for novel biocontrol methods, bacteriophages have become important candidates for the eradication of foodborne pathogens. The aim of this study was to evaluate the bacteriophage‐mediated reduction of E. coli O157:H7 in raw and filtered milk. Laboratory‐scale tests showed ...
DOI:
10.1111/jfs.12747

10. Hospital Discharge Data Underascertain Enteric Bacterial Infections Among Children

Author:
Scallan Walter, Elaine J.; McLean, Huong Q.; Griffin, Patricia M.
Source:
Foodborne pathogens & disease 2020
ISSN:
1556-7125
Subject:
Escherichia coli O157,   etc   ; Campylobacter; Salmonella; bacterial infections; children; confidence interval; enteropathogens; gastroenteritis; health services; hospitals; odds ratio; patients;     Show all 12 Subjects
Abstract:
... Many enteric pathogens disproportionately infect children. Hospital discharge data can provide information on severe infections, including cost. However, the diagnosis must be recorded on the discharge record and coded accurately. We estimated the rate of underascertainment in hospital discharge data among children with culture-confirmed Campylobacter, Salmonella, and Escherichia coli O157 infecti ...
DOI:
10.1089/fpd.2019.2773

11. Decontamination of irrigation water using a combined sand filtration and UV‐C light treatment

Author:
Sherman‐Wood, Robert; Tikekar, Rohan V.
Source:
Journal of food safety 2020 v.40 no.1 pp. e12744
ISSN:
0149-6085
Subject:
Escherichia coli O157,   etc   ; chlorination; decontamination; filtration; food safety; irrigation water; sand; sand filters; surface irrigation; turbidity; ultraviolet radiation;     Show all 11 Subjects
Abstract:
... Surface irrigation water is a food safety risk due to susceptibility to contamination and varying turbidity that affects chlorination efficacy. Effectiveness of a combined high‐throughput, commercially available sand filter and UV‐C system to inactivate Escherichia coli (K12, and attenuated O157:H7) in simulated irrigation water of varying turbidity and actual irrigation water was investigated. Sa ...
DOI:
10.1111/jfs.12744

12. Thermal inactivation of Escherichia coli O157:H7 strains and Salmonella spp. in camel meat burgers

Author:
Osaili, Tareq M.; Hasan, Fayeza; Dhanasekaran, Dinesh Kumar; Obaid, Reyad S.; Al-Nabulsi, Anas A.; Rao, Sowmya; Fatima, Hera; Ayyash, Mutamed; Savvaidis, Ioannis; Holley, Richard
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2020 v.120 pp. 108914
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; Salmonella; camel meat; camels; heat inactivation; heat treatment; lean meat; livestock and meat industry; pouches;     Show all 9 Subjects
Abstract:
... The study aimed to determine D and z-values of Escherichia coli O157:H7 strains and Salmonella spp. in camel lean meat burger (CM) and camel meat burger with hump fat (CMF). Two E. coli O157:H7 strains, S. Typhimurium and S. Copenhagen were mixed individually with CM and CMF samples, placed in sterile pouches and exposed to water bath heat treatment at 55, 57.5, 60, 62.5, 65, 67.5 °C for different ...
DOI:
10.1016/j.lwt.2019.108914

13. A sensitive electrochemical strategy via multiple amplification reactions for the detection of E. coli O157: H7

Author:
Li, Yan; Liu, Huamin; Huang, Hui; Deng, Jun; Fang, Lichao; Luo, Jing; Zhang, Shu; Huang, Jian; Liang, Wenbin; Zheng, Junsong
Source:
Biosensors & bioelectronics 2020 v.147 pp. 111752
ISSN:
0956-5663
Subject:
Escherichia coli O157,   etc   ; DNA; biosensors; detection limit; early diagnosis; electrochemistry; hybridization chain reaction; microorganisms; nucleotide sequences; virulent strains;     Show all 10 Subjects
Abstract:
... The sensitive and efficient strategy remains a central challenge for early diagnosis of pathogenic bacteria. Herein, an ultrasensitive electrochemical biosensor was proposed based on the multiple amplification strategy via the 3D DNA walker, rolling circle amplification (RCA) and hybridization chain reaction (HCR) for the accurate detection of Escherichiacoli O157:H7 (E. coli O157:H7). Firstly, th ...
DOI:
10.1016/j.bios.2019.111752

14. Combination of bacteria concentration and DNA concentration for rapid detection of E. coli O157:H7, L. monocytogenes, and S. Typhimurium without microbial enrichment

Author:
Kim, Jin-Hee; Jung, Sun; Oh, Se-Wook
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2020 v.117
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; DNA; Listeria monocytogenes; bacteria; beef; detection limit; filtration; food pathogens; lettuce; quantitative polymerase chain reaction; rapid methods;     Show all 11 Subjects
Abstract:
... Microbial enrichment of bacteria is essential for detection because generally foodborne pathogens exist on food at very low concentrations. This study described a rapid and simple pretreatment method, combination of bacteria concentration and DNA concentration for detection E. coli O157:H7, L. monocytogenes, and S. Typhimurium on lettuce and beef. This method is based on filtration, modified PrepM ...
DOI:
10.1016/j.lwt.2019.108609

15. Resistance of biofilm formation and formed-biofilm of Escherichia coli O157:H7 exposed to acid stress

Author:
Wang, Huhu; Wang, Xianjing; Yu, Lanlin; Gao, Feng; Jiang, Yun; Xu, Xinglian
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2020 v.118 pp. 108787
ISSN:
0023-6438
Subject:
Escherichia coli O157,   etc   ; acid tolerance; biofilm; lactic acid; pH; plankton; polystyrenes; scanning electron microscopy; serotypes; stress response;     Show all 10 Subjects
Abstract:
... O157 is the most common serogroup of Enterohemorrhagic Escherichia coli responsible for the most outbreaks, partly due to its acid tolerance. Biofilm formation of E. coli O157:H7 and its response to lactic acid stress were determined in this study. Results showed that the biofilm formation of 11 isolates greatly depended on the strain; specifically, the J29 isolate showed strong capacity. As compa ...
DOI:
10.1016/j.lwt.2019.108787

16. A Novel Aqueous Ozone Treatment as a Spray Chill Intervention against Escherichia coli O157:H7 on Surfaces of Fresh Beef

Author:
Kalchayanand, Norasak; Worlie, Drew; Wheeler, Tommy
Source:
Journal of food protection 2019 v.82 no.11 pp. 1874-1878
ISSN:
0362-028X
Subject:
Escherichia coli O157,   etc   ; aerobes; beef; beef cattle; muscles; ozonation; ozone;     Show all 7 Subjects
Abstract:
... This experiment determined the efficacy of using a novel aqueous ozone treatment as a spray chill intervention to reduce Escherichia coli O157:H7 on surfaces of fresh beef compared with traditional water spray chill. Cutaneous trunci muscles were obtained from a local beef cattle processing plant. Muscles were divided into sixteen 25-cm2 sections, and each section was individually inoculated with ...
DOI:
10.4315/0362-028X.JFP-19-093

17. A novel aqueous ozone treatment as a spray chill intervention against Escherichia coli O157:H7 on surfaces of fresh beef

Author:
Kalchayanand, Norasak; Worlie, Drew; Wheeler, Tommy
Source:
Journal of food protection 2019 v.82 no.11 pp. 1874-1878
ISSN:
0362-028X
Subject:
Escherichia coli O157,   etc   ; aerobes; beef; beef cattle; muscles; ozonation; ozone;     Show all 7 Subjects
Abstract:
... This experiment determined the efficacy of using a novel aqueous ozone treatment as a spray chill intervention to reduce Escherichia coli O157:H7 on surfaces of fresh beef compared with traditional water spray chill. Cutaneous trunci muscles were obtained from a local beef cattle processing plant. Muscles were divided into sixteen 25-cm2 sections, and each section was individually inoculated with ...
DOI:
10.4315/0362-028XJFP-19-093

18. A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs

Author:
Macori, Guerrino; McCarthy, Siobhán C.; Burgess, Catherine M.; Fanning, Séamus; Duffy, Geraldine
Source:
Journal of microbiological methods 2019
ISSN:
0167-7012
Subject:
Escherichia coli O157,   etc   ; pathogens; quantitative polymerase chain reaction; serotypes; sheep;     Show all 5 Subjects
Abstract:
... A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values against the initial concentration of pathogen in the sheep sample. ...
DOI:
10.1016/j.mimet.2019.105703

19. Ultrabright fluorescent microsphere and its novel application for improving the sensitivity of immunochromatographic assay

Author:
Zhang, Gang-gang; Xu, Shao-lan; Xiong, Yong-hua; Duan, Hong; Chen, Wen-yao; Li, Xiang-min; Yuan, Mei-fang; Lai, Wei-hua
Source:
Biosensors & bioelectronics 2019 v.135 pp. 173-180
ISSN:
0956-5663
Subject:
Escherichia coli O157,   etc   ; biosensors; detection limit; fluorescence; immunoaffinity chromatography; microparticles;     Show all 6 Subjects
Abstract:
... Fluorescent microsphere (FM) is widely used as probe in immunochromatographic assay (ICA). However, the performance of conventional FM is limited because of the aggregation-caused quenching effect. Herein, we compared a kind of conventional FM (DMFFM, loading DMF) with novel aggregation-induced emission FM (AIEFM, loading TCBPE). The fluorescence intensity of DMFFM initially increased and then dec ...
DOI:
10.1016/j.bios.2019.04.023

20. Characteristics of lytic phage vB_EcoM-ECP26 and reduction of shiga-toxin producing Escherichia coli on produce romaine

Author:
Park, Do-Won; Lim, Ga-yeon; Lee, Young-duck; Park, Jong-Hyun
Source:
Applied biological chemistry 2020 v.63 no.1 pp. 19
ISSN:
2468-0834
Subject:
Escherichia coli O157,   etc   ; Myoviridae; diarrhea; genes; hemolytic uremic syndrome; host range; humans; lettuce; nucleotides; open reading frames; pH; pathogens; phages; phylogeny; temperature; viral morphology;     Show all 16 Subjects
Abstract:
... Foodborne Shiga toxin-producing Escherichia coli (STEC) cause severe diarrhea and hemolytic uremic syndrome (HUS) in humans. However, traditional methods for STEC sterilization are difficult to apply to fresh food. To control the pathogen, phage infecting E. coli O157:H7 were isolated and characterized. The isolated phage vB_EcoM-ECP26 had an icosahedral head and a contractile tail, and was classi ...
DOI:
10.1186/s13765-020-00502-4