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- Fei, Mingyue, et al. Show all 7 Authors
- Biotechnology letters 2019 v.41 no.2 pp. 293-303
- DNA fragmentation; Escherichia coli; calcium chloride; electroporation; gene editing; genome
- ... OBJECTIVES: To develop a convenient chemical transformation mediated CRISPR/Cas9 (CT-CRISPR/Cas9) system for genome editing in Escherichia coli. RESULTS: Here, we have constructed a CT-CRISPR/Cas9 system, which can precisely edit bacterial genome (replacing, deleting, inserting or point mutating a target gene) through chemical transformation. Compared with the traditional electroporation mediated ...