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1. Clonal reproduction assured by sister chromosome pairing in dojo loach, a teleost fish

Author:
Fujimoto, Takafumi,   et al.   ; Kuroda, Masamichi; Murakami, Masaru; Yamaha, Etsuro; Arai, Katsutoshi;     Show all 5 Authors
Source:
Chromosome research 2018 v.26 no.4 pp. 243-253
ISSN:
0967-3849
Subject:
Misgurnus anguillicaudatus; asexual reproduction; chromosome pairing; chromosomes; clones; diploidy; endopolyploidy; females; fish; fluorescence in situ hybridization; germ cells; hybridization; hybrids; males; meiosis; ribosomal RNA; Japan
Abstract:
... Wild-type dojo loach (Misgurnus anguillicaudatus) commonly reproduces bisexually as a gonochoristic diploid (2n = 50), but gynogenetically reproducing clonal diploid lines (2n = 50) exist in certain districts in Japan. Clones have been considered to develop from past hybridization event(s) between two genetically diverse groups, A and B, within the species. Fluorescence in situ hybridization analy ...
DOI:
10.1007/s10577-018-9581-4

2. Genome incompatibility between rainbow trout (Oncorhynchus mykiss) and sea trout (Salmo trutta) and induction of the interspecies gynogenesis

Author:
Fujimoto, Takafumi,   et al.   ; Polonis, Marcin; Dobosz, Stefan; Zalewski, Tomasz; Ocalewicz, Konrad;     Show all 5 Authors
Source:
Journal of applied genetics 2018 v.59 no.1 pp. 91-97
ISSN:
1234-1983
Subject:
Oncorhynchus mykiss; Salmo trutta; alleles; chromosomes; diploidy; doubled haploids; eggs; genomics; gynogenesis; heterozygosity; high pressure treatment; homozygosity; hybrids; karyotyping; larvae; meiosis; progeny; spermatozoa; trout; ultraviolet radiation
Abstract:
... Rainbow trout (Oncorhynchus mykiss Walbaum) and sea trout (Salmo trutta Linnaeus, 1758) show large karyotypic differences and their hybrid offspring is not viable due to unstable karyotype and chromosome fragmentation. However, gametes from these two species were used to induce gynogenetic development. Rainbow trout eggs activated by UV-irradiated sea trout sperm were subjected to high hydrostatic ...
DOI:
10.1007/s13353-017-0425-2

3. Novel decaplex PCR assay for simultaneous detection of scallop species with species‐specific primers targeting highly variable 5′ end of the 16S rRNA gene

Author:
Fujimoto, Takafumi,   et al.   ; Marín, Alan; Villegas‐Llerena, Claudio; Arai, Katsutoshi;     Show all 4 Authors
Source:
Aquaculture research 2017 v.48 no.3 pp. 920-930
ISSN:
1355-557X
Subject:
DNA primers; Pectinidae; fisheries; genes; interspecific variation; mariculture; mitochondria; polymerase chain reaction; product authenticity; research institutions; ribosomal RNA; scallops; seafoods; sequence analysis; surveys
Abstract:
... Scallops (family Pectinidae) comprise species of high commercial value, supporting both commercial fisheries and mariculture activities. Accurate and reliable molecular methods for the species level identification are of outstanding utility for taxonomic and food authentication surveys. The mitochondrial 16S rRNA gene has been used to design species‐specific primers for identification of different ...
DOI:
10.1111/are.12935

4. Spontaneous polyploidization in critically endangered Acipenser mikadoi

Author:
Fujimoto, Takafumi,   et al.   ; Havelka, Miloš; Zhou, He; Hagihara, Seishi; Ichimura, Masaki; Yamaha, Etsuro; Adachi, Shinji; Arai, Katsutoshi;     Show all 8 Authors
Source:
Fisheries science 2017 v.83 no.4 pp. 587-595
ISSN:
0919-9268
Subject:
Acipenser mikadoi; Huso dauricus; chromosomes; correspondence analysis; females; fish; genetic markers; haplotypes; hybridization; hybrids; males; microsatellite repeats; mitochondria; polyploidy; progeny
Abstract:
... We investigated the source of spontaneous polyploidization in the critically endangered Acipenser mikadoi. Fourteen sib progeny of A. mikadoi and 11 hybrids between an A. mikadoi female and a Huso dauricus male, all showing atypically high ploidy, were analysed. Parent assignment based on five highly polymorphic microsatellite markers confirmed spontaneous duplication of maternal chromosome sets v ...
DOI:
10.1007/s12562-017-1083-3

5. Non-motile tetraploid spermatozoa of Misgurnus loach hybrids

Author:
Fujimoto, Takafumi,   et al.   ; Zhao, Yan; Pšenička, Martin; Saito, Taiju; Arai, Katsutoshi;     Show all 5 Authors
Source:
Fisheries science 2016 v.82 no.1 pp. 127-135
ISSN:
0919-9268
Subject:
DNA; Misgurnus anguillicaudatus; adenosine triphosphate; diploidy; energy; females; flagellum; hybrids; males; meiosis; microtubules; spermatozoa; spermiogenesis; tetraploidy; viability
Abstract:
... We have compared various properties of spermatozoa from the wild diploid male pond loach Misgurnus anguillicaudatus to those from the interspecific male hybrid of the cross between a female M. anguillicaudatus and a male mud loach M. mizolepis. Our results show that spermatozoa from this interspecific hybrid had poor motility, low viability, abnormal morphology, a larger volume of mitochondrial ma ...
DOI:
10.1007/s12562-015-0939-7

6. The mitochondrial genomes of Pecten albicans and Pecten maximus (Bivalvia: Pectinidae) reveal a novel gene arrangement with low genetic differentiation

Author:
Fujimoto, Takafumi,   et al.   ; Marín, Alan; Arai, Katsutoshi;     Show all 3 Authors
Source:
Biochemical systematics and ecology 2015 v.61 pp. 208-217
ISSN:
0305-1978
Subject:
Argopecten; Pecten maximus; amino acids; genes; genetic variation; mitochondria; mitochondrial genome; phylogeny; scallops; sequence analysis; transfer RNA
Abstract:
... Scallops (Bivalvia: Pectinidae) comprise more than 350 extant taxa including species of high economic importance. However, its phylogenetic classification remains unclear and so far only 7 scallop mitogenomes have been determined. In this study, the mitochondrial genomes of two congeneric scallop species Pecten albicans and Pecten maximus were determined. Both mitogenomes contain 12 protein-coding ...
DOI:
10.1016/j.bse.2015.06.015

7. Triploid or hybrid tetra: Which is the ideal sterile host for surrogate technology?

Author:
Fujimoto, Takafumi,   et al.   ; Piva, Lucas Henrique; de Siqueira-Silva, Diógenes Henrique; Goes, Caio Augusto Gomes; Saito, Taiju; Dragone, Letícia Veroni; Senhorini, José Augusto; Porto-Foresti, Fabio; Ferraz, José Bento Sterman; Yasui, George Shigueki;     Show all 10 Authors
Source:
Theriogenology 2018 v.108 pp. 239-244
ISSN:
0093-691X
Subject:
Astyanax altiparanae; Hyphessobrycon; Oligosarcus; crossing; diploidy; epithelium; females; fish; gametogenesis; gonads; heat stress; hybrids; males; niches; oocytes; progeny; somatic cells; spermatogonia; spermatozoa; triploidy
Abstract:
... This work was aimed at developing an effective procedure to obtain sterile ideal host fish in mass scale with no endogenous germ cells in the germinal epithelium, owning permanent stem-cell niches able to be colonized by transplanted germ cells in surrogate technology experiments. Thus, triploids, diploid hybrids, and triploid hybrids were produced. To obtain hybrid offspring, oocytes from a singl ...
DOI:
10.1016/j.theriogenology.2017.12.013

8. Utility of different sugar extenders for cryopreservation and post-thaw storage of sperm from Salmonidae species

Author:
Fujimoto, Takafumi,   et al.   ; Nynca, Joanna; Judycka, Sylwia; Liszewska, Ewa; Dobosz, Stefan; Grudniewska, Joanna; Arai, Katsutoshi; Ciereszko, Andrzej;     Show all 8 Authors
Source:
Aquaculture 2016 v.464 pp. 340-348
ISSN:
0044-8486
Subject:
Oncorhynchus mykiss; Salmo salar; Salmo trutta; Salvelinus fontinalis; cryopreservation; females; freezing; glucose; hatcheries; methanol; salmon; semen; sperm motility; spermatozoa; sucrose; thawing; trehalose
Abstract:
... The effect of replacement of glucose in glucose-methanol extender (GM) with either sucrose and trehalose on the sperm motility characteristics of cryopreserved semen and the post-thaw storage of salmon, brown trout, brook trout, sex-reversed female rainbow trout and whitefish was investigated. Semen samples were diluted 1:5 or 1:9 (for sex-reversed females) in 9% methanol containing 0.18M glucose, ...
DOI:
10.1016/j.aquaculture.2016.07.014

9. Development of nuclear DNA markers to characterize genetically diverse groups of Misgurnus anguillicaudatus and its closely related species

Author:
Fujimoto, Takafumi,   et al.   ; Yamada, Aya; Kodo, Yukihiro; Nakaya, Kohei; Okubo-Murata, Michiko; Saito, Taiju; Ninomiya, Kazuto; Inaba, Michiko; Kuroda, Masamichi; Arai, Katsutoshi; Murakami, Masaru;     Show all 11 Authors
Source:
Fisheries science 2017 v.83 no.5 pp. 743-756
ISSN:
0919-9268
Subject:
DNA; DNA primers; Misgurnus anguillicaudatus; genes; genetic markers; hybrids; introgression; mitochondrial genome; nuclear genome; nucleotide sequences; polymerase chain reaction; restriction fragment length polymorphism
Abstract:
... Repetitive DNA sequences, ManDra and ManBgl, were isolated from the DraI and BglII digests of the genomic DNA of Misgurnus anguillicaudatus, respectively. A primer set of ManDra distinguished two genetically different groups (A and B) of M. anguillicaudatus by specific electrophoretograms. A primer set of ManBgl amplified the DNA of M. anguillicaudatus and M. mizolepis. The individuals of M. angui ...
DOI:
10.1007/s12562-017-1108-y

10. Growth, fatty acid composition, and reproductive parameters of diploid and triploid yellowtail tetra Astyanax altiparanae

Author:
Fujimoto, Takafumi,   et al.   ; do Nascimento, Nivaldo Ferreira; Pereira-Santos, Matheus; Piva, Lucas Henrique; Manzini, Breno; Senhorini, José Augusto; Yasui, George Shigueki; Nakaghi, Laura Satiko Okada;     Show all 8 Authors
Source:
Aquaculture 2017 v.471 pp. 163-171
ISSN:
0044-8486
Subject:
Astyanax altiparanae; aquaculture; carcass yield; diploidy; fatty acid composition; females; fins; fish; gonads; heat stress; males; monounsaturated fatty acids; polyunsaturated fatty acids; rearing; spermatozoa; sport fishing; triploidy
Abstract:
... The aim of this study was to evaluate the growth, carcass yield, fatty acid composition, and reproductive parameters of diploid and triploid Astyanax altiparanae. Triploidization was induced by heat shock (40°C for 2min) 2min after fertilization. Fish were reared for 175days from fertilization. Triploid females showed lower proportion of saturated and poly-unsaturated fatty acids and higher amount ...
DOI:
10.1016/j.aquaculture.2017.01.007

11. Rapid species identification of fresh and processed scallops by multiplex PCR

Author:
Fujimoto, Takafumi,   et al.   ; Marín, Alan; Arai, Katsutoshi;     Show all 3 Authors
Source:
Food control 2013 v.32 no.2 pp. 472-476
ISSN:
0956-7135
Subject:
Argopecten irradians; Argopecten purpuratus; Mizuhopecten yessoensis; Pecten; food safety; genes; issues and policy; polymerase chain reaction; ribosomal RNA; scallops; seafoods; species identification
Abstract:
... Food control policies regarding to seafood label authenticity have become a global issue due to increased incidence of species substitution or mislabelling. Proper species-level identification in processed scallop products is hindered by the lack of morphological characters such as their valves. In order to identify four commercially important scallop species (Argopecten purpuratus, Argopecten irr ...
DOI:
10.1016/j.foodcont.2013.01.007

12. Genetic structure of the Peruvian scallop Argopecten purpuratus inferred from mitochondrial and nuclear DNA variation

Author:
Fujimoto, Takafumi,   et al.   ; Marín, Alan; Arai, Katsutoshi;     Show all 3 Authors
Source:
Marine genomics 2013 v.9 pp. 1-8
ISSN:
1874-7787
Subject:
Argopecten purpuratus; genes; genetic markers; genetic variation; haplotypes; microsatellite repeats; mitochondrial DNA; nuclear genome; ribosomal RNA; scallops; statistical analysis
Abstract:
... The population genetic structure of the Peruvian scallop Argopecten purpuratus from three different wild populations along the Peruvian coast was analyzed using nine microsatellite loci and a partial region (530bp) of the mitochondrial 16S rRNA gene. A total of 19 polymorphic sites in the 16S rRNA gene defined 18 unique haplotypes. High genetic diversity was presented in all populations. Statistic ...
DOI:
10.1016/j.margen.2012.04.007
PubMed:
23904058

13. Androgenetic doubled haploids induced without irradiation of eggs in loach (Misgurnus anguillicaudatus)

Author:
Fujimoto, Takafumi,   et al.   ; Hou, Jilun; Saito, Taiju; Yamaha, Etsuro; Arai, Katsutoshi;     Show all 5 Authors
Source:
Aquaculture 2014 v.420-421 pp. S57
ISSN:
0044-8486
Subject:
Misgurnus anguillicaudatus; alleles; color; diploidy; doubled haploids; eggs; females; flow cytometry; heterozygosity; homozygosity; irradiation; linkage groups; loci; males; microsatellite repeats; spermatozoa
Abstract:
... Androgenetic doubled haploids (DHs) were induced in the loach Misgurnus anguillicaudatus (Cobitidae) without irradiation of the eggs. The eggs of wild-type females were activated with the intact sperm of an orange-phenotype male, and treated (within 10s of activation) at 3±0.5°C for 30min, to eliminate the female nucleus. The eggs were then incubated in a water bath at 20±0.5°C for 35min. Finally, ...
DOI:
10.1016/j.aquaculture.2013.05.021

14. Isolation and characterization of 12 microsatellite loci in the Peruvian scallop Argopecten purpuratus and cross-species amplification in other scallop species (family Pectinidae)

Author:
Fujimoto, Takafumi,   et al.   ; Marín, Alan; Arai, Katsutoshi;     Show all 3 Authors
Source:
Conservation genetics resources 2012 v.4 no.1 pp. 179-182
ISSN:
1877-7252
Subject:
Argopecten purpuratus; alleles; loci; microsatellite repeats; scallops
Abstract:
... Argopecten purpuratus is an economically important marine bivalve. Twelve microsatellite loci were isolated and characterized in 20 individuals of A. purpuratus collected in Isla Independencia (Pisco, Peru) and tested for cross-species amplification in other 4 scallops (family Pectinidae). Ten loci were polymorphic. The number of alleles per locus ranged from 2 to 13. The observed (H O) and expect ...
DOI:
10.1007/s12686-011-9503-7

15. Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Author:
Fujimoto, Takafumi,   et al.   ; Adamov, Nadya Soares de Macedo; Nascimento, Nivaldo Ferreira do; Maciel, Elayna Cristina Silva; Pereira‐Santos, Matheus; Senhorini, José Augusto; Calado, Leonardo Luiz; Evangelista, Mariana Machado; Nakaghi, Laura Satiko Okada; Guerrero, Alan Hertz Marín; Yasui, George Shigueki;     Show all 11 Authors
Source:
Journal of the World Aquaculture Society 2017 v.48 no.5 pp. 741-750
ISSN:
0893-8849
Subject:
Astyanax altiparanae; aquaculture; cold stress; eggs; erythrocytes; fish; flow cytometry; haploidy; hatching; heat stress; karyotyping; temperature; triploidy
Abstract:
... Triploidization is an interesting tool to produce sterile fish. In the yellowtail tetra, Astyanax altiparanae, this can be applied for aquaculture and surrogate technologies. In this study, we compared the efficacy of cold (2 C) or heat shock (38 C, 40 C, and 42 C) on triploid induction in the yellowtail tetra. The eggs were treated with cold or heat shock, 2 min postfertilization (30 min in cold ...
DOI:
10.1111/jwas.12390

16. Heat-shock-induced tetraploid and diploid/tetraploid mosaic in pond loach, Misgurnus anguillicaudatus

Author:
Fujimoto, Takafumi,   et al.   ; Sakao, Suzu; Oshima, Kouzou; Yamaha, Etsuro; Arai, Katsutoshi;     Show all 5 Authors
Source:
Aquaculture international 2013 v.21 no.4 pp. 769-781
ISSN:
0967-6120
Subject:
Misgurnus anguillicaudatus; aneuploidy; breeding; cell division; diploidy; eggs; fish; haploidy; hatching; hexaploidy; insemination; larvae; males; ponds; pressure treatment; spermatozoa; tetraploidy; triploidy
Abstract:
... Tetraploid fish, which are considered as key resources of diploid gametes for further breeding and ploidy manipulation, can be artificially induced by inhibition of the mitotic cell division with hydrostatic pressure or temperature treatments. Although many attempts have been made to induce artificial tetraploid strains, successful establishment of viable and fertile tetraploid strains are rare. I ...
DOI:
10.1007/s10499-012-9581-x

17. Production of androgenetic diploid loach by cold-shock of eggs fertilized with diploid sperm

Author:
Fujimoto, Takafumi,   et al.   ; Hou, Jilun; Yamaha, Etsuro; Arai, Katsutoshi;     Show all 4 Authors
Source:
Theriogenology 2013 v.80 no.2 pp. 125-130
ISSN:
0093-691X
Subject:
Misgurnus anguillicaudatus; diploidy; eggs; females; fish; flow cytometry; genetic markers; hatching; irradiation; larvae; spermatozoa
Abstract:
... Diploid androgenotes were produced without egg irradiation in the loach, Misgurnus anguillicaudatus. Eggs of wild-type diploid females were fertilized with diploid sperm of a neo-tetraploid male and then cold-shock treated at 3 °C (range, ±0.5 °C) for 30 minutes just after fertilization to eliminate the female nucleus. After hatching, ploidy status of the hatched larvae was analyzed by flow cytome ...
DOI:
10.1016/j.theriogenology.2013.03.014
PubMed:
23602217

18. Restoration of the loach, Misgurnus anguillicaudatus, from cryopreserved diploid sperm and induced androgenesis

Author:
Fujimoto, Takafumi,   et al.   ; Yasui, George Shigueki; Arai, Katsutoshi;     Show all 3 Authors
Source:
Aquaculture 2010 v.308 pp. S140
ISSN:
0044-8486
Subject:
Misgurnus anguillicaudatus; androgenesis; bovine serum albumin; cryopreservation; diploidy; egg quality; females; fish culture; fish eggs; genotype; hatching; inactivation; irradiation; larvae; microsatellite repeats; progeny; salmon; seminal plasma; spermatozoa; tetraploidy
Abstract:
... In the present study, we evaluated the feasibility of using cryopreserved diploid sperm as a repository genebank for the loach, Misgurnus anguillicaudatus, along with a rederivation strategy utilizing induced-androgenesis. Firstly, we evaluated three types of media for egg inactivation: Hank's saline solution+0.5% bovine serum albumin (BSA), Ringer's solution+0.5% BSA, and masu salmon seminal plas ...
DOI:
10.1016/j.aquaculture.2010.05.041

19. Germ cells are not the primary factor for sexual fate determination in goldfish

Author:
Fujimoto, Takafumi,   et al.   ; Goto, Rie; Saito, Taiju; Takeda, Takahiro; Takagi, Misae; Arai, Katsutoshi; Yamaha, Etsuto;     Show all 7 Authors
Source:
Developmental biology 2012 v.370 no.1 pp. 98-109
ISSN:
0012-1606
Subject:
Danio rerio; adults; animal ovaries; aromatase; chimerism; embryogenesis; females; genes; goldfish; males; oligonucleotides; phenotype; progeny; seminiferous tubules; sex determination; sex reversal; somatic cells; spermatozoa
Abstract:
... The presence of germ cells in the early gonad is important for sexual fate determination and gonadal development in vertebrates. Recent studies in zebrafish and medaka have shown that a lack of germ cells in the early gonad induces sex reversal in favor of a male phenotype. However, it is uncertain whether the gonadal somatic cells or the germ cells are predominant in determining gonadal fate in o ...
DOI:
10.1016/j.ydbio.2012.07.010

20. The effect of ions and cryoprotectants upon sperm motility and fertilization success in the loach Misgurnus anguillicaudatus

Author:
Fujimoto, Takafumi,   et al.   ; Yasui, George Shigueki; Arias-Rodriguez, Lenin; Takagi, Yasuaki; Arai, Katsutoshi;     Show all 5 Authors
Source:
Aquaculture 2012 v.344-349 pp. 147-152
ISSN:
0044-8486
Subject:
Misgurnus anguillicaudatus; buffers; ions; methanol; calcium; fertilizer rates; dimethyl sulfoxide; salts; sperm motility; cryoprotectants; magnesium; potassium; spermatozoa
Abstract:
... The solutions commonly used to dilute or cryopreserve sperm are commonly composed of salts, buffers and cryoprotectants, which may affect gametes and subsequent fertilization success. Here, we have evaluated the effects of several cryoprotectants (methanol; MeOH, dimethyl sulfoxide; DMSO and dimethyl acetamide; DMA at concentrations of 0.25, 0.5 and 1%) and different ions (potassium, calcium and m ...
DOI:
10.1016/j.aquaculture.2012.03.005