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Gallidalphaherpesvirus1, etc ; etiological agents; nucleotide sequences; respiratory tract diseases; restriction fragment length polymorphism; virology; Show all 6 Subjects
Abstract:
... Infectious laryngotracheitis virus (ILTV) is the causative agent of an economically important disease of chickens causing upper respiratory tract infection. Strains of ILTV are commonly identified by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and/or PCR high resolution melt (PCR-HRM) curve analysis targeting several genes. However, these techniques examine only a ...
Gallidalphaherpesvirus1, etc ; B-lymphocytes; DNA; antigens; cell structures; density gradient centrifugation; erythrocytes; fluorescence; glycoproteins; macrophages; monocytes; virulence; viruses; Show all 13 Subjects
Abstract:
... Infectious laryngotracheitis virus (ILTV) DNA has been detected in blood fractions, but the cell phenotype with which the virus is associated is unknown. This study investigated the presence of ILTV antigen in peripheral blood cells of six acutely ILTV-infected chickens (5 or 9 days post ocular inoculation with a virulent isolate) and three sham-inoculated chickens using immunofluorescent staining ...
... Infectious laryngotracheitis (ILT) is a disease of high economic consequence to the poultry sector. Gallid herpesvirus 1 (GaHV-1), a.k.a infectious laryngotracheitis virus (ILTV), under the genus Iltovirus, and the family Herpesviridae, is the agent responsible for the disease. Despite the clinical signs on the field suggestive of ILT, it has long been considered nonexistent and a disease of no co ...
Gallidalphaherpesvirus1, etc ; amino acids; chorioallantoic membrane; genes; phylogeny; respiratory tract diseases; restriction fragment length polymorphism; sequence analysis; vaccines; virology; viruses; India; Show all 12 Subjects
Abstract:
... Infectious laryngotracheitis (ILT) is an acute respiratory disease in chickens that is a serious threat to poultry-producing countries worldwide. In the present study, we isolated and characterized infectious laryngotracheitis (ILTV) virus isolates by sequencing and restriction fragment length polymorphism analysis of PCR-amplified products (PCR-RFLP). A total of 26 ILTV outbreaks were investigate ...
... Infectious laryngotracheitis virus (ILTV) causes severe respiratory disease in chickens and results in huge economic losses in the poultry industry worldwide. To correlate the genomic difference with the replication and pathogenicity, phenotypes of three ILTVs isolated from chickens in China from 2016 to 2018 were sequenced by high-throughput sequencing. Based on the entire genome, the isolates GD ...
... BACKGROUND: Infectious laryngotracheitis (ILT) and infectious bronchitis (IB) are two common respiratory diseases of poultry that inflict great economic burden on the poultry industry. Developing an effective agent against both viruses is a crucial step to decrease the economic losses. Therefore, for the first time green synthesized silver nanoparticles using Cyperus rotundus L. aqueous extract wa ...
Gallidalphaherpesvirus1, etc ; air; air sacs; animal pathology; conjunctiva; cytoplasm; epithelium; giant cells; histopathology; immunohistochemistry; larynx; lungs; mucus; necropsy; respiratory tract diseases; tissue distribution; viral antigens; Show all 17 Subjects
Abstract:
... Infectious laryngotracheitis (ILT) is an important upper respiratory disease of chickens. Gross and histologic lesions of ILT in chickens are compared to immunohistochemistry to evaluate the diagnostic test sensitivity. A total of 31 separate ILT-confirmed necropsy submissions (12 commercial meat-type flocks, 13 egg-type producers, and 6 backyard flocks) were arbitrarily selected. Each submission ...
... The aim of this study was to develop a microarray assay for the simultaneous detection of the H5, H7, H9, N1, N9 and N2 genes of the avian influenza virus (AIV) using a Nanogold-streptavidin and silver-stain-enhanced nucleic acid dot-blot hybridisation system. The conserved sequences of H5 genes from H5N1, H7 genes from H7N9, H9 genes from H9N2, N9 genes from H7N9 and N2 genes from H9N2 AIV were c ...
Gallidalphaherpesvirus1, etc ; biotin; birds; cross reaction; genes; poultry science; temperature; Show all 7 Subjects
Abstract:
... The purpose of this study was to explore a specific, simple, and sensitive method for diagnosis of avian infectious laryngotracheitis virus. Recombinase-aided amplification (RAA) and lateral flow dipstick (LFD) were combined for labeling the optimized RAA probe with 6-carboxyfluorescein (FAM) and the 5′-end of the downstream primer with biotin, respectively. By optimizing the reaction time, temper ...
... Despite the high cost of vaccination programmes, conventional methods to evaluate vaccine uptake are often impractical and costly. More recently, molecular-based testing of poultry dust has been used to monitor the “take” of Marek's disease virus and infectious laryngotracheitis virus (ILTV) live vaccines. This study aimed to provide proof-of-concept for detecting other poultry pathogens by using ...
Gallidalphaherpesvirus1, etc ; T-lymphocytes; chick embryos; mucosa; tissue culture; vaccination; virulence; virus replication; viruses; Show all 9 Subjects
Abstract:
... The types of immune cells that populate the trachea after ILTV vaccination and infection have not been assessed. The objective of this study was to quantify CD4⁺, CD8α⁺, CD8β⁺, TCRγδ⁺, and MRC1LB⁺ cells that infiltrate the trachea after vaccination with chicken embryo origin (CEO), tissue culture origin (TCO), and recombinant herpesvirus of turkey-laryngotracheitis (rHVT-LT) vaccines, and after ch ...
Gallidalphaherpesvirus1, etc ; Gallid alphaherpesvirus 2; Meleagrid alphaherpesvirus 1; chickens; complement; glycoproteins; host specificity; viruses; Show all 8 Subjects
Abstract:
... Marek’s disease (MD) in chickens is caused by Gallid alphaherpesvirus 2, better known as MD herpesvirus (MDV). Current vaccines do not block interindividual spread from chicken-to-chicken, therefore, understanding MDV interindividual spread provides important information for the development of potential therapies to protect against MD, while also providing a natural host to study herpesvirus disse ...
... Gallid alpha-herpesvirus 1, also known as avian infectious laryngotracheitis virus (ILTV), continues to cause huge economic losses to the poultry industry worldwide. Similar to that of other herpesvirus-encoded proteins, the expression of viral genes encoded by ILTV is regulated by a cascade, and the underlying regulatory mechanism remains largely unclear. The viral immediate-early (IE) gene ICP4 ...
Gallidalphaherpesvirus1, etc ; cell lines; distress; eyes; genes; hatcheries; live vaccines; seroconversion; vaccination; viral load; virulence; viruses; Show all 12 Subjects
Abstract:
... To produce more-stable, live attenuated vaccines for infectious laryngotracheitis virus (ILTV), deletion of genes related to virulence has been extensively pursued. Although its function remains unknown, the open reading frame C (ORF C) is among the genes potentially associated with viral virulence that is nonessential for replication in vitro. Earlier results indicated that the ILT virus with del ...
... Infectious laryngotracheitis (ILT), fowlpox (FP), and reticuloendotheliosis are important poultry diseases caused by gallid herpesvirus 1 (ILTV), fowlpox virus (FWPV), and reticuloendotheliosis virus (REV), respectively. Coinfections with ILTV and FWPV occur naturally in chickens, and FP in its more virulent wet form is characterized by diphtheritic lesions and easily confused with ILT. Moreover, ...
Gallidalphaherpesvirus1, etc ; amino acids; chick embryos; genetic variance; genome; infection; live vaccines; phylogeny; poultry; sequence analysis; virulence; Ontario; Quebec; Show all 13 Subjects
Abstract:
... Infectious laryngotracheitis (ILT), caused by infectious laryngotracheitis virus (ILTV), occurs sporadically in poultry flocks in Canada. Live attenuated chicken embryo origin (CEO) vaccines are being used routinely to prevent and control ILTV infections. However, ILT outbreaks still occur since vaccine strains could revert to virulence in the field. In this study, 7 Canadian ILTV isolates linked ...
... Infectious laryngotracheitis (ILT) is an economically crucial respiratory disease of poultry that affects the industry worldwide. Vaccination is the principal tool in the control of the disease outbreak. In an earlier study, we comprehensively characterized the circulating strains in Egypt and identified both CEO-like and recombinant strains are dominant. Herein, we investigated the pathogenicity ...
Gallidalphaherpesvirus1, etc ; Herpesviridae infections; cell lines; chickens; genome; liquid chromatography; mass spectrometry; metabolism; metabolome; microbiology; models; poultry industry; probability; therapeutics; transcriptomics; Show all 15 Subjects
Abstract:
... Therapeutics targeting virus–host interactions have been considered promising strategies for treating herpesvirus infection. Our previous study on avian infectious laryngotracheitis virus (ILTV), an avian herpesvirus economically important to the poultry industry worldwide, identified the small molecule Pifithrin-α (PFT-α) as a potential therapeutic agent. However, the underlying mechanisms of its ...
... The ability of infectious laryngotracheitis virus (ILTV) to replicate in organs outside of the upper respiratory tract and conjunctiva associated-lymphoid tissues is still not well understood. This study investigated the tissue distribution of an Australian field strain of ILTV (class 9) on birds experimentally inoculated via eye-drop at 7 days of age by using quantitative PCR (qPCR) and immunohis ...
Gallidalphaherpesvirus1, etc ; chicken industry; chickens; genomics; human resources; loop-mediated isothermal amplification; organ-on-a-chip; quantitative polymerase chain reaction; respiratory system; viruses; Show all 10 Subjects
Abstract:
... Infectious laryngotracheitis (ILT) is a viral disease of chickens’ respiratory system that imposes considerable financial burdens on the chicken industry. Rapid, simple, and specific detection of this virus is crucial to enable proper control measures. Polymerase chain reaction (PCR)-based molecular tests require relatively expensive instruments and skilled personnel, confining their application t ...