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... MON 87460 (D1) maize contains a gene that expresses the cold shock protein B (CSPB) from Bacillus subtilis to confer a yield advantage when yield is limited by water availability. This study evaluated the composition of grain from the D1-containing combined-trait maize hybrids D1 Ã NK603, D1 Ã MON 89034 Ã NK603, and D1 Ã MON 89034 Ã MON 88017. These stacks offer a combination of insect protec ...
Zea mays; starch; discriminant analysis; community development; alleles; amino acids; beta-carotene; chromosome mapping; corn; data collection; fatty acids; hybrids; inbred lines; landraces; minerals; phytic acid; plant breeding; raffinose; tocopherols; New York; South America
Abstract:
... The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America. The NAM founders represent a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis o ...
Bayesian theory; case studies; corn; crops; germplasm; probability distribution; safety assessment; soybeans; transgenic plants
Abstract:
... New transgenic (GM) crops are subjected to extensive safety assessments that include compositional comparisons with conventional counterparts as a cornerstone of the process. The influence of germplasm, location, environment, and agronomic treatments on compositional variability is, however, often obscured in these pair-wise comparisons. Furthermore, classical statistical significance testing can ...
Ostrinia nubilalis; Zea mays; adverse effects; amino acids; corn; genetic background; glyphosate; growing season; hybrids; insect pests; metabolites; minerals; nutritive value; plant cultural practices; seeds; sugars; sweetcorn; vitamins; United States
Abstract:
... Monsanto Co. has developed two sweet corn hybrids, MON 88017 and MON 89034, that contain biotechnology-derived (biotech) traits designed to enhance sustainability and improve agronomic practices. MON 88017 confers benefits of glyphosate tolerance and protection against corn rootworm. MON 89034 provides protection against European corn borer and other lepidopteran insect pests. The purpose of this ...
... 1H NMR spectroscopy offers advantages in metabolite quantitation and platform robustness when applied in food metabolomics studies. This paper provides a 1H NMR-based assessment of seed metabolomic diversity in conventional and glyphosate-resistant genetically modified (GM) soybean from a genetic lineage representing â¼35 years of breeding and differing yield potential. 1H NMR profiling of harves ...
GeorgeG. Harrigan, et al. ; Melissa M. Thompson; Ashley Niemuth; Jane Sabbatini; David Levin; Matthew L. Breeze; Xin Li; Timothy Perez; Mary Taylor; Show all 9 Authors
breeding; germplasm; guidelines; high performance liquid chromatography; phylloquinone; soybeans; transgenic plants; United States
Abstract:
... Recommendations by the Organization of Economic Cooperation and Development (OECD) for compositional assessments of new genetically modified (GM) soybean now include measurement of vitamin K₁. Here we employ an HPLC method to provide information on variation in levels of vitamin K₁ in seed from soybean representing over 35 years of breeding. Vitamin K₁ analysis was conducted on seed from six conve ...
... Conventional maize hybrid seed production has historically relied upon detasseling using either manual methods or semiautomated processes to ensure the purity of the hybrid cross. Monsanto Co. has developed biotechnology-derived MON 87427 maize with tissue-selective glyphosate tolerance to facilitate the production of hybrid maize seed. MON 87427 utilizes a specific promoter and intron combination ...
GeorgeG. Harrigan, et al. ; Tyamagondlu V. Venkatesh; Alexander W. Chassy; Oliver Fiehn; Sherry Flint-Garcia; Qin Zeng; Kirsten Skogerson; Show all 7 Authors
agronomic traits; alleles; chromosome mapping; corn; corn ears; correlation; gas chromatography-mass spectrometry; genetic variation; germplasm; hybrids; landraces; metabolites; metabolomics; nuclear magnetic resonance spectroscopy; nutrient content; South America
Abstract:
... The present study expands metabolomic assessments of maize beyond commercial lines to include two sets of hybrids used extensively in the scientific community. One set included hybrids derived from the nested association mapping (NAM) founder lines, a collection of 25 inbreds selected on the basis of genetic diversity and used to investigate the genetic basis of complex plant traits. A second set ...
GeorgeG. Harrigan, et al. ; Tyamagondlu V. Venkatesh; Erin Bell; Anna Bickel; Kevin Cook; Benjamin Alsop; Martijn van de Mortel; Ping Feng; Alan Willse; Tim Perez; Susan Riordan; Show all 11 Authors
... In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait. To demonstrate this, four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines ...
... Here, we show that differences between genetically modified (GM) and non‐GM comparators cannot be attributed unequivocally to the GM trait, but arise because of minor genomic differences in near‐isogenic lines. Specifically, this study contrasted the effect of three GM traits (drought tolerance, MON 87460; herbicide resistance, NK603; insect protection, MON 89034) on maize grain composition relati ...
GeorgeG. Harrigan, et al. ; Rod A. Herman; Brandon J. Fast; Peter N. Scherer; Alyssa M. Brune; Denise T. de Cerqueira; Barry W. Schafer; Ricardo D. Ekmay; Greg A. Bradfisch; Show all 9 Authors
breeding; chemical composition; corn; genetically modified plants; hybrids; transgenesis
Abstract:
... The impact of crossing (‘stacking’) genetically modified (GM) events on maize‐grain biochemical composition was compared with the impact of generating nonGM hybrids. The compositional similarity of seven GM stacks containing event DAS‐Ø15Ø7‐1, and their matched nonGM near‐isogenic hybrids (iso‐hybrids) was compared with the compositional similarity of concurrently grown nonGM hybrids and these sam ...
GeorgeG. Harrigan, et al. ; Joseph E. Huesing; David Andres; Michael P. Braverman; Andrea Burns; Allan S. Felsot; Richard L. Hellmich; Alan Reynolds; Anthony M. Shelton; Wilna Jansen van Rijssen; E. Jane Morris; Jacobus N. Eloff; Show all 12 Authors
... Advances in biotechnology continue to drive the development of a wide range of insect-protected, herbicide-tolerant, stress-tolerant, and nutritionally enhanced genetically modified (GM) crops, yet societal and public policy considerations may slow their commercialization. Such restrictions may disproportionately affect developing countries, as well as smaller entrepreneurial and public sector ini ...
GeorgeG. Harrigan, et al. ; Miyako Kusano; Ivan Baxter; Atsushi Fukushima; Akira Oikawa; Yozo Okazaki; Ryo Nakabayashi; Denise J. Bouvrette; Frederic Achard; Andrew J. Jakubowski; Joan M. Ballam; Jonathan R. Phillips; Angela H. Culler; Kazuki Saito; Show all 14 Authors
DNA fingerprinting; Glycine max; capillary electrophoresis; crops; gas chromatography; genetic engineering; glyphosate; herbicide resistance; ionomics; liquid chromatography; mass spectrometry; metabolites; metabolomics; plant breeding; seeds; soybeans; transgenic plants; variance; United States
Abstract:
... Information on crop genotype- and phenotype-metabolite associations can be of value to trait development as well as to food security and safety. The unique study presented here assessed seed metabolomic and ionomic diversity in a soybean lineage representing ~35 years of breeding (launch years 1972-2008) and increasing yield potential. Selected varieties included six conventional and three glyphos ...
Datasets:
Data from: Assessing metabolomic and chemical diversity of a soybean lineage representing 35 years of breeding