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1. Peroxidase zymograms obtained by agarose native gel electrophoresis have unmet resolution and completeness

Author:
Giulia Annovi,   et al.   ; Sandra Oloketuyi; Ario de Marco;     Show all 3 Authors
Source:
International journal of biological macromolecules 2020 v.156 pp. 869-873
ISSN:
0141-8130
Subject:
acrylamides; agarose; biomarkers; gel electrophoresis; gels; horseradish; peroxidase; protein isoforms; staining
Abstract:
... A recently published method to separate protein isoforms by means of a flat-bed agarose native gel was adapted for identifying simultaneously both acid and basic isoforms of plant peroxidases. These were evidenced by in situ activity staining using alternative substrates for which the isoforms showed specific preference. Such approach allowed the detection of a significantly higher number of horse ...
DOI:
10.1016/j.ijbiomac.2020.04.058