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- Gomes, Joana Castro, et al. Show all 7 Authors
- Bioresource technology 2019 v.280 pp. 37-50
- biomass; biorefining; enzymatic reactions; enzymes; partition coefficients; physical phases; polymers; robots; solutes; sugars
- ... Aqueous two-phase systems (ATPS) can be applied to enzymatic reactions that are affected by product inhibition. In the biorefinery context, sugars inhibit the cellulolytic enzymes in charge of converting the biomass. Here, we present a strategy to select an ATPS (formed by polymer and salt) that can separate sugar and enzymes. This automated and miniaturized method is able to determine phase diagr ...