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1. Red drupelet reversion in blackberries caused by mechanical damage is not linked to a reduction in anthocyanin content

Author:
Gonzalez-de la Vara, Luis E.,   et al.   ; Flores-Sosa, Angel R.; Soto-Magaña, Diana; Sanchez-Segura, Lino; Bah, Moustapha; Rivera-Pastrana, Dulce M.; Nava, Gerardo M.; Mercado-Silva, Edmundo M.;     Show all 8 Authors
Source:
Postharvest biology and technology 2021 v.180 pp. 111618
ISSN:
0925-5214
Subject:
anthocyanins; blackberries; color; cultivars; fluorescence; fruits; mechanical damage; microscopy; models; transportation; vibration
Abstract:
... Red Drupelet Reversion (RDR) in blackberry is a physiological disorder where numerous fruit drupelets regress to red from black color during post-harvest handling. The specific mechanism causing RDR is still unknown; however, numerous studies have suggested that anthocyanin contents could be involved in this phenomenon. Recently, it has been reported that RDR in cultivars ‘Ouachita’, ‘Triple Crown ...
DOI:
10.1016/j.postharvbio.2021.111618

2. Fructan active enzymes (FAZY) activities and biosynthesis of fructooligosaccharides in the vacuoles of Agave tequilana Weber Blue variety plants of different age

Author:
González de la Vara, Luis E.,   et al.   ; Mellado-Mojica, Erika; López, Mercedes G.;     Show all 3 Authors
Source:
Planta 2017 v.245 no.2 pp. 265-281
ISSN:
0032-0935
Subject:
Agave tequilana; biochemical pathways; biosynthesis; developmental stages; enzymes; fructans; fructooligosaccharides; isomers; models; plant age; polymerization; protoplasts; vacuoles; Mexico
Abstract:
... MAIN CONCLUSION : Biosynthesis of agave fructans occurs in mesontle vacuoles which showed fluctuations in FAZY activities and synthesized a diverse spectrum of fructooligosaccharide isomers. Agave tequilana Weber Blue variety is an important agronomic crop in Mexico. Fructan metabolism in A. tequilana exhibits changes in fructan content, type, degree of polymerization (DP), and molecular structure ...
DOI:
10.1007/s00425-016-2602-7

3. Protein phosphorylation regulates in vitro spinach chloroplast petD mRNA 3′-untranslated region stability, processing, and degradation

Author:
González de la Vara, Luis E.,   et al.   ; Vargas-Suárez, Martín; Castro-Sánchez, Alina; Toledo-Ortiz, Gabriela; García, Elpidio; Loza-Tavera, Herminia;     Show all 6 Authors
Source:
Biochimie 2013 v.95 pp. 400-409
ISSN:
0300-9084
Subject:
RNA-binding proteins; adenosine triphosphate; binding properties; chloroplasts; messenger RNA; metabolism; protein phosphorylation; spinach
Abstract:
... RNA-binding proteins (RNPs) participate in diverse processes of mRNA metabolism, and phosphorylation changes their binding properties. In spinach chloroplasts, 24RNP and 28RNP are associated with polynucleotide posphorylase forming a complex on charge of pre-mRNA 3′-end maturation. Here, we tested the hypothesis that the phosphorylation status of 24RNP and 28RNP, present in a spinach chloroplast m ...
DOI:
10.1016/j.biochi.2012.10.012

4. Isolation of detergent-resistant membranes from plant photosynthetic and non-photosynthetic tissues

Author:
González de la Vara, Luis E.,   et al.   ; Carmona-Salazar, Laura; El Hafidi, Mohammed; Enríquez-Arredondo, Consuelo; Vázquez-Vázquez, Christian; Gavilanes-Ruíz, Marina;     Show all 6 Authors
Source:
Analytical biochemistry 2011 v.417 no.2 pp. 220-227
ISSN:
0003-2697
Subject:
Nicotiana tabacum; Phaseolus vulgaris; Zea mays; corn; developmental stages; eukaryotic cells; leaves; plasma membrane; sphingolipids; sterols; tissues; ultrastructure
Abstract:
... Microdomains, or lipid rafts, are transient membrane regions enriched in sphingolipids and sterols that have only recently, but intensively, been studied in plants. In this work, we report a detailed, easy-to-follow, and fast procedure to isolate detergent-resistant membranes (DRMs) from purified plasma membranes (PMs) that was used to obtain DRMs from Phaseolus vulgaris and Nicotiana tabacum leav ...
DOI:
10.1016/j.ab.2011.05.044

5. Purification and characterization of a calcium-dependent protein kinase from beetroot plasma membranes

Author:
González de la Vara, Luis E.,   et al.   ; Lino, Bárbara; Carrillo-Rayas, M. Teresa; Chagolla, Alicia;     Show all 4 Authors
Source:
Planta 2006 v.225 no.1 pp. 255-268
ISSN:
0032-0935
Subject:
Arabidopsis thaliana; Beta vulgaris; adenosine triphosphate; beets; centrifugation; chromatography; gels; glycerol; isoelectric point; mass spectrometry; pH; phosphorylase kinase; plasma membrane; rice; transporters
Abstract:
... Several calcium-dependent protein kinases (CDPKs) are located in plant plasma membranes where they phosphorylate enzymes and transporters, like the H⁺-ATPase and water channels, thereby regulating their activities. In order to determine which kinases phosphorylate the H⁺-ATPase, a calcium-dependent kinase was purified from beetroot (Beta vulgaris L.) plasma membranes by anion-exchange chromatograp ...
DOI:
10.1007/s00425-006-0343-8
PubMed:
16868777