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- Grünweller, Arnold, et al. Show all 4 Authors
- Nucleic acids research 2010 v.38 no.14 pp. e147
- Northern blotting; RNA; bacteria; crosslinking; polyacrylamide gel electrophoresis
- ... Here we describe a northern blot procedure that allows the detection of endogenous RNAs as small as ~14 nt in total RNA extracts from bacteria. RNAs that small and as part of total bacterial RNA extracts usually escape detection by northern blotting. The approach combines LNA probes 5'-digoxigenin-endlabeled for non-radioactive probe detection with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide-me ...
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