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- Grommen, A.W.F., et al. Show all 6 Authors
- Nucleic acids research 2008 v.36 no.22 pp. e151
- Escherichia coli; Rhodococcus equi; cholesterol; cholesterol metabolism; cytosine deaminase; cytotoxicity; fluorouracil; gene deletion; macrophages; methodology; mortality; multigene family; mutants; nucleic acids; pathogens; phenotype; uracil
- ... A novel method to efficiently generate unmarked in-frame gene deletions in Rhodococcus equi was developed, exploiting the cytotoxic effect of 5-fluorocytosine (5-FC) by the action of cytosine deaminase (CD) and uracil phosphoribosyltransferase (UPRT) enzymes. The opportunistic, intracellular pathogen R. equi is resistant to high concentrations of 5-FC. Introduction of Escherichia coli genes encodi ...
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