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- Author:
- Guoyuan Wen, et al. ; Xin Wang; Yiluo Cheng; Wenting Zhang; Qin Lu; Qingping Luo; Huabin Shao; Zishu Pan; Tengfei Zhang; Show all 9 Authors
- Source:
- Microbiological research 2021 v.245 pp. 126685
- ISSN:
- 0944-5013
- Subject:
- DNA replication; RNA; Salmonella Pullorum; adhesion; biofilm; death; ligases; mutants; osmolarity; pathogenicity islands; pathogens; poultry industry; protein synthesis; pullorum disease; research; secretion; stress response; virulence
- Abstract:
- ... Salmonella Pullorum is a pathogen specific to birds that can cause Pullorum disease in young chickens and lead to considerable economic losses in the poultry industry. During transmission and infection, S. Pullorum will encounter various environmental stresses and host defenses. The stringent response is an important adaptation response induced by (p)ppGpp, and in Salmonella, (p)ppGpp is synthesiz ...
- DOI:
- 10.1016/j.micres.2020.126685
- https://doi.org/10.1016/j.micres.2020.126685
- Author:
- Guoyuan Wen, et al. ; Rongrong Zhang; Yan He; Xinyu Zhu; Qingping Luo; Tengfei Zhang; Qin Lu; Shudan Liu; Shaobo Xiao; Liurong Fang; Huabin Shao; Show all 11 Authors
- Source:
- Biochemical and biophysical research communications 2021 v.561 pp. 52-58
- ISSN:
- 0006-291X
- Subject:
- Tembusu virus; antiviral proteins; ducks; fibroblasts; innate immunity; polypeptides; research; spleen; zinc finger motif
- Abstract:
- ... This is the first study to clone duck CCCH-type zinc finger antiviral protein (duZAP) from Jingjiang duck (Anas platyrhynchos). Full-length duZAP cDNA was 2154 bp and encoded a 717-amino acid polypeptide containing four highly conserved CCCH-type finger motifs, a WWE domain and a poly (ADP-ribose) polymerase (PARP) domain. duZAP was expressed in multiple duck tissues, with the highest mRNA express ...
- DOI:
- 10.1016/j.bbrc.2021.05.019
- https://doi.org/10.1016/j.bbrc.2021.05.019
- Author:
- Guoyuan Wen, et al. ; Sanling Fan; Yulun Wu; Hongling Wang; Yu Shang; Qingping Luo; Tengfei Zhang; Rongrong Zhang; Wenting Zhang; Ling Luo; Huabin Shao; Show all 11 Authors
- Source:
- Poultry science 2020 v.99 no.5 pp. 2438-2443
- ISSN:
- 0032-5791
- Subject:
- Avian orthoavulavirus 1; Newcastle disease; active immunity; antibodies; cell-mediated immunity; chickens; passive immunity; specific pathogen-free animals; vaccination; vaccines; virulence; viruses
- Abstract:
- ... Maternally derived antibodies (MDA) substantially interfere with active immunity in post-hatch vaccination, although they provide early protection against disease through passive immunity in young chickens. Previously, Newcastle disease virus (NDV) strain TS09-C was demonstrated to be safe and immunogenic as in-ovo vaccine in specific-pathogen-free chickens. Here, we evaluated the safety, protecti ...
- DOI:
- 10.1016/j.psj.2020.01.006
- https://doi.org/10.1016/j.psj.2020.01.006
- Author:
- Guoyuan Wen, et al. ; Lulai Xu; Zhenqiao Qin; Lei Qiao; Jie Wen; Huabin Shao; Zishu Pan; Show all 7 Authors
- Source:
- Vaccine 2020 v.38 no.7 pp. 1690-1699
- ISSN:
- 0264-410X
- Subject:
- Avian orthoavulavirus 1; Influenza A virus; chickens; complementary DNA; developing countries; gene transfer; growth models; hemagglutinins; pathogenicity; poultry industry; secretion; signal peptide; t-plasminogen activator; thermal stability; vaccination; vaccine development; vaccines; viruses
- Abstract:
- ... Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene delivery. In the present study, we generated the thermostable recombinant NDV (rNDV) expressing the different forms of hemagglutinin (HA) of highly pathogenic avian influenza virus (HPAIV) H5N1 based on the full-length cDNA clone of thermostable TS09-C strain. The recombinant thermostable Newcastle dise ...
- DOI:
- 10.1016/j.vaccine.2019.12.054
- https://doi.org/10.1016/j.vaccine.2019.12.054
- Author:
- Guoyuan Wen, et al. ; Yiluo Cheng; Zhang Sihua; Qin Lu; Wenting Zhang; Qingping Luo; Huabin Shao; Tengfei Zhang; Show all 8 Authors
- Source:
- Avian pathology 2020 v.49 no.5 pp. 507-514
- ISSN:
- 1465-3338
- Subject:
- Salmonella Pullorum; airborne transmission; bioaerosols; chickens; dose response; hemorrhage; histopathology; inflammation; interleukin-6; interleukin-8; lungs; morbidity; pathogenicity; pathogens; poultry industry; spleen
- Abstract:
- ... Salmonella enterica serovar Pullorum (S. Pullorum) is an important pathogen specific to avian species, which poses a serious threat to the poultry industry. The transmission of S. Pullorum occurs both horizontally and vertically but the airborne transmission of S. Pullorum has been neglected historically. In this study, the effects of aerosolized S. Pullorum on young chickens were investigated. Th ...
- DOI:
- 10.1080/03079457.2020.1783433
- https://doi.org/10.1080/03079457.2020.1783433
- Author:
- Guoyuan Wen, et al. ; Xin Wang; Honglin Wang; Tingting Li; Feifei Liu; Yiluo Cheng; Xiaodong Guo; Qingping Luo; Huabin Shao; Zishu Pan; Tengfei Zhang; Show all 11 Authors
- Source:
- BMC veterinary research 2020 v.16 no.1 pp. 299
- ISSN:
- 1746-6148
- Subject:
- Salmonella Enteritidis; Salmonella Gallinarum; Salmonella Typhimurium; accounting; ampicillin; antibiotic resistance; assays; chickens; ciprofloxacin; colistin; correlation; developing countries; genes; genetic variation; genotype; hosts; information; meropenem; multilocus sequence typing; multiple drug resistance; pathogens; poultry industry; salmonellosis; serotypes; veterinary medicine; virulence; China
- Abstract:
- ... BACKGROUND: Salmonella is an important zoonotic pathogen, and chickens are one of its main hosts. Every year, Salmonella infections pose a serious threat to the poultry industry in developing countries, especially China. In this study, a total of 84 Salmonella isolates recovered from sick and healthy-looking chickens in central China were characterized by serotyping, MLST-based strain typing, pres ...
- DOI:
- 10.1186/s12917-020-02513-1
- https://doi.org/10.1186/s12917-020-02513-1
- Author:
- Guoyuan Wen, et al. ; Qingzhong Yu; Stephen Spatz; Yufeng Li; Jilong Yang; Wei Zhao; Zhenyu Zhang; Maricarmen Garcia; Laszlo Zsak; Show all 9 Authors
- Source:
- Vaccine 2017 v.35 no.5 pp. 789-795
- ISSN:
- 0264-410X
- Subject:
- neutralization; broiler chickens; hemagglutination; Gallid alphaherpesvirus 1; antibodies; vaccines; secondary immunization; Avian orthoavulavirus 1; tissues; maternal immunity; virulence; glycoproteins
- Abstract:
- ... Newcastle disease virus (NDV) recombinants expressing the infectious laryngotracheitis virus (ILTV) glycoproteins B and D have previously been demonstrated to confer complete clinical protection against virulent ILTV and NDV challenges in naive chickens. We extended this study to assess whether maternally derived antibody (MDA) against NDV and ILTV would interfere with protection in vaccinated bro ...
- Handle:
- 10113/5603951
- DOI:
- 10.1016/j.vaccine.2016.12.038
- CHORUS:
- 10.1016/j.vaccine.2016.12.038
- Chorus Open Access:
- 10.1016/j.vaccine.2016.12.038
- https://doi.org/10.1016/j.vaccine.2016.12.038
8. Evaluation of a thermostable Newcastle disease virus strain TS09-C as an in-ovo vaccine for chickens
- Author:
- Guoyuan Wen, et al. ; Lintao Li; Qingzhong Yu; Hongling Wang; Qingping Luo; Tengfei Zhang; Rongrong Zhang; Wanpo Zhang; Huabin Shao; Show all 9 Authors
- Source:
- Plos One 2017 v.12 no.2 pp. -
- ISSN:
- 1932-6203
- Subject:
- chickens; thermal stability; lesions (animal); vaccines; biosafety; vaccination; Avian orthoavulavirus 1; intestines; hatching; tissues; immune response; trachea (vertebrates); viruses; strains; virulence; histopathology; poultry industry
- Abstract:
- ... In-ovo vaccination is an attractive immunization approach for poultry industry. However, most of the Newcastle disease virus (NDV) vaccine strains used after hatch are unsafe, as in-ovo vaccines, due to their high pathogenicity for chicken embryos. In this study, we evaluated the safety and immunogenicity of a thermostable NDV strain TS09-C, derived from V4 strain, as in-ovo vaccine. Chickens in-o ...
- Handle:
- 10113/5801872
- DOI:
- 10.1371/journal.pone.0172812
- PubMed:
- 28234989
- PubMed Central:
- PMC5325573
- https://doi.org/10.1371/journal.pone.0172812
- Author:
- Guoyuan Wen, et al. ; Wei Zhao; Stephen Spatz; Zhenyu Zhang; Maricarmen Garcia; Laszlo Zsak; Qingzhong Yu; Show all 7 Authors
- Source:
- Journal of virology 2014 v.88 no.15 pp. 8397-8406
- ISSN:
- 0022-538X
- Subject:
- disease prevention; Avian orthoavulavirus 1; vaccination; broiler chickens; Gallid alphaherpesvirus 1; aerosols; viruses; virulence; glycoproteins; genetic recombination; vaccine development; drinking water; fluorescent antibody technique; adverse effects; tissue culture; weight gain; protein synthesis; specific pathogen-free animals; live vaccines; viral proteins; recombinant vaccines; genetic vectors
- Abstract:
- ... Infectious laryngotracheitis (ILT) is a highly contagious acute respiratory disease of chickens caused by infectious laryngotracheitis virus (ILTV). The disease is controlled mainly through biosecurity and vaccination with live attenuated strains of ILTV and vectored vaccines based on turkey herpesvirus (HVT) and fowlpox virus (FPV). The current live attenuated vaccines (chicken embryo origin [CEO ...
- Handle:
- 10113/59865
- DOI:
- 10.1128/JVI.01321-14
- PubMed:
- no direct match
- PubMed Central:
- PMC4135966
- https://doi.org/10.1128/JVI.01321-14
- Author:
- Guoyuan Wen, et al. ; Ling Wu; Longfei Ding; Zenglin Pei; Xixiang Huo; Zishu Pan; Show all 6 Authors
- Source:
- Journal of virological methods 2013 v.188 no.1-2 pp. 47-50
- ISSN:
- 0166-0934
- Subject:
- Influenza A virus; RNA; agarose; birds; detection limit; gel electrophoresis; genes; hemagglutinins; humans; pathogens; rapid methods; reverse transcriptase polymerase chain reaction; screening
- Abstract:
- ... A multiplex reverse transcription-PCR (mRT-PCR) assay was developed for the rapid detection of influenza A viruses. The assay simultaneously differentiated H1, H3, H5 and H9 hemagglutinin subtypes in a single reaction mixture. Five sets of specific primers targeted to the M, H1, H3, H5 and H9 genes were used in this assay. The amplified products were visualized by agarose gel electrophoresis. The ...
- DOI:
- 10.1016/j.jviromet.2012.11.032
- https://doi.org/10.1016/j.jviromet.2012.11.032
- Author:
- Guoyuan Wen, et al. ; Xiao Hu; Kang Zhao; Hongling Wang; Zhenyu Zhang; Tengfei Zhang; Jinlong Yang; Qingping Luo; Rongrong Zhang; Zishu Pan; Huabin Shao; Qingzhong Yu; Show all 12 Authors
- Source:
- Scientifc reports 2016 v.6 no.22492 pp. 1-9
- ISSN:
- 2045-2322
- Subject:
- chickens; thermal stability; Newcastle disease; phenotype; antibodies; vaccines; chimerism; Avian orthoavulavirus 1; hemagglutinins; genes; viruses; sialidase; virulence; cold
- Abstract:
- ... Thermostable Newcastle disease virus (NDV) vaccines have been used widely to protect village chickens against Newcastle disease, due to their decreased dependence on cold chain for transport and storage. However, the genetic basis underlying the NDV thermostability is poorly understood. In this study, we generated chimeric viruses by exchanging viral genes between the thermostable TS09-C strain an ...
- Handle:
- 10113/62117
- DOI:
- 10.1038/srep22492
- PubMed:
- 26935738
- PubMed Central:
- PMC4776148
- https://doi.org/10.1038/srep22492
- Author:
- Guoyuan Wen, et al. ; Yu Shang; Jing Guo; Chen Chen; Huabin Shao; Qingping Luo; Jun Yang; Hongling Wang; Guofu Cheng; Show all 9 Authors
- Source:
- Virus genes 2013 v.46 no.3 pp. 542-545
- ISSN:
- 0920-8569
- Subject:
- sequence analysis; thermal stability; phenotype; nucleotides; amino acid sequences; Avian orthoavulavirus 1; amino acids; genes; genotype; nucleotide sequences
- Abstract:
- ... A thermostable avirulent Newcastle disease virus (NDV) strain TS09-C was developed by serial passage of V4 strain in BHK-21 cells. The complete genome sequence of strain TS09-C was determined and compared with the sequences of other NDV isolates representing different thermostable phenotypes. The TS09-C genome was 15,186 nucleotides long and consisted of eight genes in the order of 3′-NP-P/V/W-M-F ...
- DOI:
- 10.1007/s11262-012-0871-1
- PubMed:
- 23296874
- https://doi.org/10.1007/s11262-012-0871-1
- Author:
- Guoyuan Wen, et al. ; Chen Chen; Jing Guo; Zhenyu Zhang; Yu Shang; Huabin Shao; Qingping Luo; Jun Yang; Hongling Wang; Hongcai Wang; Tengfei Zhang; Rongrong Zhang; Guofu Cheng; Qingzhong Yu; Show all 14 Authors
- Source:
- Journal of General Virology 2015 v.96 pp. 1219-1228
- ISSN:
- 0022-1317
- Subject:
- thermal stability; Newcastle disease; Foot-and-mouth disease virus; Avian orthoavulavirus 1; vaccination; cells; gene expression; viruses; start codon; dynamics; heterologous gene expression; virulence; developing countries; ubiquitin; cold; chickens; translation (genetics); vaccines; villages; storage; nucleocapsid proteins
- Abstract:
- ... Thermostable Newcastle disease virus (NDV) vaccines have been used widely to control Newcastle disease for village poultry flocks, due to their independence of cold chains for delivery and storage. To explore the potential use of thermostable NDV as a vaccine vector, an infectious clone of thermostable avirulent NDV strain TS09-C was developed using reverse genetics technology. The GFP gene, along ...
- Handle:
- 10113/61395
- DOI:
- 10.1099/vir.0.000067
- https://doi.org/10.1099/vir.0.000067
- Author:
- Guoyuan Wen, et al. ; Tao Zhang; Jun Yang; Qingping Luo; Yonghong Liao; Zhibin Hu; Rongrong Zhang; Hongling Wang; Diyun Ai; Ling Luo; Nianhua Song; Huabin Shao; Show all 12 Authors
- Source:
- Journal of virological methods 2011 v.176 no.1-2 pp. 96-102
- ISSN:
- 0166-0934
- Subject:
- Classical swine fever virus; RNA; blood serum; detection limit; genotype; hog cholera; monitoring; nose; reverse transcriptase polymerase chain reaction; screening; swine; tissue culture; viruses
- Abstract:
- ... A one-step real-time RT-PCR assay using a minor groove binding probe was developed for the specific detection of Chinese wild-type classical swine fever virus (CSFV). The assay detected wild-type CSFV strains representing different genotypes, but did not amplify viral RNA from the Hog Cholera Lipinized Virus (HCLV) vaccine-strain and other porcine viruses. The assay had a detection limit of 10copi ...
- DOI:
- 10.1016/j.jviromet.2011.06.014
- https://doi.org/10.1016/j.jviromet.2011.06.014