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1. Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

Author:
Herman, Philippe,   et al.   ; Fraiture, Marie-Alice; Vandamme, Julie; Roosens, Nancy H. C.;     Show all 4 Authors
Source:
BMC biotechnology 2018 v.18 no.1 pp. 40
ISSN:
1472-6750
Subject:
European Union; genetically modified organisms; genome walking; human granulocytic ehrlichiosis; transgenes
Abstract:
... BACKGROUND: Recently, an integrated DNA walking strategy has been proposed to prove the presence of GMO via the characterisation of sequences of interest, including their transgene flanking regions and the unnatural associations of elements in their transgenic cassettes. To this end, the p35S, tNOS and t35S pCAMBIA elements have been selected as key targets, allowing the coverage of most of GMO, E ...
DOI:
10.1186/s12896-018-0446-x
PubMed:
29945581
PubMed Central:
PMC6020286

2. How Can We Better Detect Unauthorized GMOs in Food and Feed Chains?

Author:
Herman, Philippe,   et al.   ; Fraiture, Marie-Alice; De Loose, Marc; Debode, Frédéric; Roosens, Nancy H.;     Show all 5 Authors
Source:
Trends in biotechnology 2017 v.35 no.6 pp. 508-517
ISSN:
0167-7799
Subject:
DNA; genetically modified organisms; high-throughput nucleotide sequencing; nucleotide sequences
Abstract:
... Current GMO detection systems have limited abilities to detect unauthorized genetically modified organisms (GMOs). Here, we propose a new workflow, based on next-generation sequencing (NGS) technology, to overcome this problem. In providing information about DNA sequences, this high-throughput workflow can distinguish authorized and unauthorized GMOs by strengthening the tools commonly used by enf ...
DOI:
10.1016/j.tibtech.2017.03.002

3. An integrated strategy combining DNA walking and NGS to detect GMOs

Author:
Herman, Philippe,   et al.   ; Fraiture, Marie-Alice; Papazova, Nina; De Loose, Marc; Deforce, Dieter; Ruttink, Tom; Roosens, Nancy H.;     Show all 7 Authors
Source:
Food chemistry 2017 v.232 pp. 351-358
ISSN:
0308-8146
Subject:
food processing; genetically modified organisms; genome walking; high-throughput nucleotide sequencing; noodles; rice; transgenes
Abstract:
... Recently, we developed a DNA walking system for the detection and characterization of a broad spectrum of GMOs in routine analysis of food/feed matrices. Here, we present a new version with improved throughput and sensitivity by coupling the DNA walking system to Pacific Bioscience® Next-generation sequencing technology. The performance of the new strategy was thoroughly assessed through several a ...
DOI:
10.1016/j.foodchem.2017.03.067

4. Biotech rice: Current developments and future detection challenges in food and feed chain

Author:
Herman, Philippe,   et al.   ; Fraiture, Marie-Alice; Roosens, Nancy H.C.; Taverniers, Isabel; De Loose, Marc; Deforce, Dieter;     Show all 6 Authors
Source:
Trends in food science & technology 2016 v.52 pp. 66-79
ISSN:
0924-2244
Subject:
Agrobacterium radiobacter; biolistics; breeding methods; crops; gene expression; genetically modified organisms; genome walking; plant breeding; quantitative polymerase chain reaction; rice; traceability; transgenesis; Europe
Abstract:
... To improve agricultural practices and the food/feed security, plant breeding techniques were developed, including transgenesis commonly using Agrobacterium tumefaciens or biolistic technologies. To guarantee the traceability of GMO in food/feed chain and the consumer's freedom of choice, regulatory frameworks were established in many countries around the world, such as in Europe. Their implementat ...
DOI:
10.1016/j.tifs.2016.03.011

5. Engineering nucleases for gene targeting: safety and regulatory considerations

Author:
Herman, Philippe,   et al.   ; Pauwels, Katia; Podevin, Nancy; Breyer, Didier; Carroll, Dana;     Show all 5 Authors
Source:
New biotechnology 2014 v.31 pp. 18-27
ISSN:
1871-6784
Subject:
Protozoa; engineering; gene targeting; genetic engineering; genome; humans; nucleases
Abstract:
... Nuclease-based gene targeting (NBGT) represents a significant breakthrough in targeted genome editing since it is applicable from single-celled protozoa to human, including several species of economic importance. Along with the fast progress in NBGT and the increasing availability of customized nucleases, more data are available about off-target effects associated with the use of this approach. We ...
DOI:
10.1016/j.nbt.2013.07.001

6. Statistical framework for detection of genetically modified organisms based on Next Generation Sequencing

Author:
Herman, Philippe,   et al.   ; Willems, Sander; Fraiture, Marie-Alice; Deforce, Dieter; De Keersmaecker, Sigrid C.J.; De Loose, Marc; Ruttink, Tom; Van Nieuwerburgh, Filip; Roosens, Nancy;     Show all 9 Authors
Source:
Food chemistry 2016 v.192 pp. 788-798
ISSN:
0308-8146
Subject:
crops; food matrix; high-throughput nucleotide sequencing; noodles; quantitative polymerase chain reaction; rice; transgenes; transgenic plants
Abstract:
... Because the number and diversity of genetically modified (GM) crops has significantly increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly complex and laborious. While several pioneers already investigated Next Generation Sequencing (NGS) as an alternative to qPCR, its practical use has not been assessed for routine analysis. In this study a statistical framewor ...
DOI:
10.1016/j.foodchem.2015.07.074

7. Next-generation sequencing as a tool for the molecular characterisation and risk assessment of genetically modified plants: Added value or not?

Author:
Herman, Philippe,   et al.   ; Pauwels, Katia; De Keersmaecker, Sigrid C.J.; De Schrijver, Adinda; du Jardin, Patrick; Roosens, Nancy H.C.;     Show all 6 Authors
Source:
Trends in food science & technology 2015 v.45 no.2 pp. 319-326
ISSN:
0924-2244
Subject:
DNA shuffling; Southern blotting; genetically modified plants; genomics; high-throughput nucleotide sequencing; international organizations; laws and regulations; nucleic acids; polymerase chain reaction; research projects; risk assessment; surveys
Abstract:
... Legislations and international organizations provide a framework to ensure proper risk assessment of Genetically Modified Organisms (GMO). With regard to the deliberate release of GMO as food or feed, applications for Genetically Modified Plants (GMP) typically contain data for the molecular characterisation at the nucleic acid level based on Southern blot and polymerase chain reaction analysis in ...
DOI:
10.1016/j.tifs.2015.07.009

8. Validation of a sensitive DNA walking strategy to characterise unauthorised GMOs using model food matrices mimicking common rice products

Author:
Herman, Philippe,   et al.   ; Fraiture, Marie-Alice; Taverniers, Isabel; De Loose, Marc; Van Nieuwerburgh, Filip; Deforce, Dieter; Roosens, Nancy H.;     Show all 7 Authors
Source:
Food chemistry 2015 v.173 pp. 1259-1265
ISSN:
0308-8146
Subject:
DNA; detection limit; food matrix; food processing; genome walking; quantitative polymerase chain reaction; rice products; screening; transgenes; transgenic plants
Abstract:
... To identify unauthorised GMOs in food and feed matrices, an integrated approach has recently been developed targeting pCAMBIA family vectors, highly present in transgenic plants. Their presence is first assessed by qPCR screening and is subsequently confirmed by characterising the transgene flanking regions, using DNA walking. Here, the DNA walking performance has been thoroughly tested for the fi ...
DOI:
10.1016/j.foodchem.2014.09.148

9. An innovative and integrated approach based on DNA walking to identify unauthorised GMOs

Author:
Herman, Philippe,   et al.   ; Fraiture, Marie-Alice; Taverniers, Isabel; De Loose, Marc; Deforce, Dieter; Roosens, Nancy H.;     Show all 6 Authors
Source:
Food chemistry 2014 v.147 pp. 60-69
ISSN:
0308-8146
Subject:
Bacillus thuringiensis; DNA; feeds; foods; genome; polymerase chain reaction; rice; transgenic plants
Abstract:
... In the coming years, the frequency of unauthorised genetically modified organisms (GMOs) being present in the European food and feed chain will increase significantly. Therefore, we have developed a strategy to identify unauthorised GMOs containing a pCAMBIA family vector, frequently present in transgenic plants. This integrated approach is performed in two successive steps on Bt rice grains. Firs ...
DOI:
10.1016/j.foodchem.2013.09.112
PubMed:
24206686

10. Forces Driving the Attachment of Staphylococcus epidermidis to Fibrinogen-Coated Surfaces

Author:
Herman, Philippe,   et al.   ; El-Kirat-Chatel, Sofiane; Beaussart, Audrey; Geoghegan, Joan A.; Vanzieleghem, Thomas; Foster, Timothy J.; Hols, Pascal; Mahillon, Jacques; Dufrêne, Yves F.;     Show all 9 Authors
Source:
Langmuir 2013 v.29 no.42 pp. 13018-13022
ISSN:
1520-5827
Subject:
Staphylococcus epidermidis; adhesion; bacteria; bacterial adhesion; bacterial proteins; blood plasma; fibrinogen; ligands; mutants; pathogens; probability; secondary infection; spectroscopy; surface proteins; therapeutics
Abstract:
... Cell surface proteins of bacteria play essential roles in mediating the attachment of pathogens to host tissues and, therefore, represent key targets for anti-adhesion therapy. In the opportunistic pathogen Staphylococcus epidermidis, the adhesion protein SdrG mediates attachment of bacteria to the blood plasma protein fibrinogen (Fg) through a binding mechanism that is not yet fully understood. W ...
DOI:
10.1021/la4029172

11. Event report: SynBio Workshop (Paris 2012) – Risk assessment challenges of Synthetic Biology

Author:
Herman, Philippe,   et al.   ; Pauwels, Katia; Mampuys, Ruth; Golstein, Catherine; Breyer, Didier; Kaspari, Marion; Pagès, Jean-Christophe; Pfister, Herbert; van der Wilk, Frank; Schönig, Birgit;     Show all 10 Authors
Source:
Journal für Verbraucherschutz und Lebensmittelsicherheit = 2013 v.8 no.3 pp. 215-226
ISSN:
1661-5751
Subject:
biosafety; biotechnology; food safety; genetic engineering; monitoring; public health; risk assessment; synthetic biology; Netherlands
Abstract:
... In Europe and beyond, several advisory bodies have been monitoring the developments in the field of Synthetic Biology. Reports have been sent to national governments for information on the developments and possible regulatory and risk assessment questions raised by this field. To put the issues in a broader perspective, four national biosafety advisory bodies (the French High Council for Biotechno ...
DOI:
10.1007/s00003-013-0829-9