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- Hill, Shakeema, et al. Show all 8 Authors
- Analytical biochemistry 2019 v.571 pp. 21-24
- denaturation; detergents; legumes; models; polyacrylamide gel electrophoresis; proteins; sodium dodecyl sulfate
- ... Sodium dodecyl sulfate (SDS) is a detergent used as a strong denaturant of proteins in gel electrophoresis. It has previously been shown that certain hyperstable, also known as kinetically stable, proteins are resistant to SDS and thus require heating for their denaturation in the presence of SDS. Because of its high denaturing strength, relatively few proteins are resistant to SDS thereby limitin ...
- Hill, Shakeema, et al. Show all 9 Authors
- Biochemical and biophysical research communications 2017
- ambient temperature; black beans; cost effectiveness; detergents; digestibility; germination; half life; lima beans; navy beans; phaseolin; polyacrylamide gel electrophoresis; proteolysis; red beans
- ... In common beans and lima bean, the storage protein phaseolin is difficult to degrade and SDS-resistant, a sign of kinetic stability. Kinetically stable proteins (KSPs) are characterized by having a high-energy barrier between the native and denatured states that results in very slow unfolding. Such proteins are resistant to proteolytic degradation and detergents, such as SDS. Here the method SDS-T ...