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- Hou, Song-Tao, et al. Show all 6 Authors
- Molecular biology reports 2014 v.41 no.1 pp. 497-504
- Escherichia coli; Streptomyces lividans; catalytic activity; genes; industrial applications; molecular weight; pH; polyacrylamide gel electrophoresis; temperature; thermal stability; tricarboxylic acid cycle
- ... Fumarase is a key enzyme that catalyzes the reversible hydration of fumarate to L-malate in the tricarboxylic acid cycle. This reaction has been extensively utilized for industrial applications in producing L-malate. In this study, a fumarase C gene from Streptomyces lividans TK54 (slFumC) was cloned and expressed as a fused protein (SlFumC) in Escherichia coli. The molecular mass of SlFumC was ab ...