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- Author:
- James E. Wells, et al. ; Laura M. Rubeck; Kathryn J. Hanford; Lisa M. Durso; Walter H. Schacht; Elaine D. Berry; Show all 6 Authors
- Source:
- Science of the total environment 2022 v.817 pp. 152611
- ISSN:
- 0048-9697
- Subject:
- Anas platyrhynchos; Escherichia coli O157; antibiotic resistance; cattle; cattle production; ecology; environment; nitrogen; overwintering; pathogens; rain; streams; water quality
- Abstract:
- ... The impacts of management-intensive grazing (MIG) of cattle on concentrations of total Escherichia coli, total suspended solids (TSS), and nitrate-nitrite nitrogen (NO₃ + NO₂-N), and occurrence of E. coli O157:H7 and selected antibiotic resistance genes (ARGs) in stream water and/or sediments were evaluated. Cattle were grazed for two-week periods in May in each of three years. Overall, grazing in ...
- Handle:
- 10113/7622425
- DOI:
- 10.1016/j.scitotenv.2021.152611
- CHORUS:
- 10.1016/j.scitotenv.2021.152611
- https://doi.org/10.1016/j.scitotenv.2021.152611
- Author:
- James E. Wells, et al. ; Nathan S. Long; Elaine D. Berry; Jerrad F. Legako; Dale R. Woerner; Guy H. Loneragan; Paul R. Broadway; Jeff A. Carroll; Nicole C. Burdick Sanchez; Samodha C. Fernando; Carley M. Bacon; Cory L. Helmuth; Taylor M. Smock; Jeff L. Manahan; Ashley A. Hoffman; Kristin E. Hales; Show all 16 Authors
- Source:
- Journal of applied microbiology 2022 v.133 no.3 pp. 1940-1955
- ISSN:
- 1364-5072
- Subject:
- Enterococcus; Escherichia coli; Salmonella enterica; antibiotic resistance; beef cattle; ceftiofur; crossbreds; feces; feedlots; florfenicol; lymph; microbiology
- Abstract:
- ... AIMS: Our objective was to determine how injectable antimicrobials affected populations of Salmonella enterica, Escherichia coli and Enterococcus spp. in feedlot cattle. METHODS AND RESULTS: Two arrival date blocks of high‐risk crossbred beef cattle (n = 249; mean BW = 244 kg) were randomly assigned one of four antimicrobial treatments administered on day 0: sterile saline control (CON), tulathrom ...
- DOI:
- 10.1111/jam.15691
- https://doi.org/10.1111/jam.15691
- Author:
- James E. Wells, et al. ; Brittney N. Keel; Amanda K. Lindholm-Perry; William T. Oliver; Shuna A. Jones; Lea A. Rempel; Show all 6 Authors
- Source:
- BMC genomic data 2021 v.22 no.1 pp. 25
- ISSN:
- 2730-6844
- Subject:
- bioinformatics; colostrum; digestive system; epithelium; exosomes; gene expression regulation; gene ontology; genomics; immune response; immunoglobulins; innate immunity; lactation; linear models; macrophages; mammary glands; neonates; neutrophils; piglets; transcription (genetics); transcriptomics; whole milk
- Abstract:
- ... BACKGROUND: Porcine milk is a complex fluid, containing a myriad of immunological, biochemical, and cellular components, made to satisfy the nutritional requirements of the neonate. Whole milk contains many different cell types, including mammary epithelial cells, neutrophils, macrophages, and lymphocytes, as well nanoparticles, such as milk exosomes. To-date, only a limited number of livestock tr ...
- DOI:
- 10.1186/s12863-021-00980-5
- https://doi.org/10.1186/s12863-021-00980-5
- Author:
- James E. Wells, et al. ; Elaine D. Berry; Lisa M. Durso; Kristina M. Friesen; James L. Bono; Trevor V. Suslow; Show all 6 Authors
- Source:
- Journal of food protection 2019 v.82 no.8 pp. 1300-1307
- ISSN:
- 0362-028X
- Subject:
- Calliphoridae; Escherichia coli O157; Musca autumnalis; Musca domestica; Sarcophagidae; Stomoxys calcitrans; beef cattle; cattle production; crops; disease transmission; feces; feedlots; foodborne illness; green leafy vegetables; insect pests; pathogens; pulsed-field gel electrophoresis; relative risk; risk reduction; sticky traps
- Abstract:
- ... Leafy greens are leading vehicles for Escherichia coli O157:H7 foodborne illness. Pest flies can harbor this pathogen and may disseminate it to produce. We determined the occurrence of E. coli O157:H7–positive flies in leafy greens planted up to 180 m from a cattle feedlot and assessed their relative risk to transmit this pathogen to leafy greens. The primary fly groups captured on sticky traps at ...
- Handle:
- 10113/6662304
- DOI:
- 10.4315/0362-028X.JFP-18-601
- https://doi.org/10.4315/0362-028X.JFP-18-601
- Author:
- James E. Wells, et al. ; Elaine D. Berry; Show all 2 Author
- Source:
- Burleigh Dodds Science Publishing Limitied 2016 v.1 no. pp. -
- Subject:
- adulterated products; animal husbandry; animal pathogens; beef; direct contact; economic incentives; food pathogens; food processing; food sanitation; foodborne illness; humans; zoonoses
- Abstract:
- ... Mankind has long recognized that animals harbor disease. Zoonotic pathogens are agents from animals that cause disease in humans. The pathogens may be broad spectrum and cause disease in animals and humans, or the animal may simply be an asymptomatic reservoir for a human pathogen. The human disease can occur as a result of direct contact between the animal and human or transmitted through a vecto ...
- Handle:
- 10113/5801797
- DOI:
- 10.19103/AS.2016.0008.01
- https://doi.org/10.19103/AS.2016.0008.01
- Author:
- James. E. Wells, et al. ; Minseok Kim; Show all 2 Author
- Source:
- Current microbiology 2016 v.72 no.2 pp. 145-151
- ISSN:
- 0343-8651
- Subject:
- Firmicutes; bacterial communities; cattle; data collection; databases; feces; meta-analysis; nucleotide sequences; ribosomal RNA; sequence homology; species diversity
- Abstract:
- ... In this study, we conducted a meta-analysis on 16S rRNA gene sequences of bovine fecal origin that are publicly available in the RDP database. A total of 13,663 sequences including 603 isolate sequences were identified in the RDP database (Release 11, Update 1), where 13,447 sequences were assigned to 10 phyla, 17 classes, 28 orders, 59 families, and 110 genera, while the remaining 216 sequences c ...
- Handle:
- 10113/61798
- DOI:
- 10.1007/s00284-015-0931-6
- https://doi.org/10.1007/s00284-015-0931-6
- Author:
- James E. Wells, et al. ; Mindy J. Spiehs; Elaine D. Berry; David B. Parker; Tami M. Brown‐Brandl; Show all 5 Authors
- Source:
- Journal of environmental quality 2017 v.46 no.4 pp. 722-732
- ISSN:
- 0047-2425
- Subject:
- Escherichia coli; Pinus; Zea mays; antibacterial properties; bacteria; beef; carbon nitrogen ratio; cattle; corn; corn stover; emissions; litter (bedding); nutrient content; odor compounds; odors; soil microorganisms; sulfides; sulfur; volatile organic compounds; wood chips
- Abstract:
- ... Pine (Pinus spp.) bedding has been shown to lower the concentration of odorous volatile organic compounds (VOCs) and pathogenic bacteria compared with corn (Zea mays L.) stover bedding, but availability and cost limit the use of pine bedding in cattle confinement facilities. The objectives of this study were to determine if the addition of pine wood chips to laboratory‐scaled bedded packs containi ...
- Handle:
- 10113/5801884
- DOI:
- 10.2134/jeq2016.09.0333
- https://doi.org/10.2134/jeq2016.09.0333
- Author:
- James E. Wells, et al. ; Kristin E. Hales; Elaine D. Berry; Norasak Kalchayanand; James L. Bono; Min-Seok Kim; Show all 6 Authors
- Source:
- Journal of animal science 2017 v.95 no.8 pp. 3738-3744
- ISSN:
- 0021-8812
- Subject:
- Escherichia coli O157; bacterial shedding; beef cattle; diet; feces; finishing; growth performance; heifers; models; monensin; steers
- Abstract:
- ... Two experiments were conducted to study the effects of monensin dose on growth performance and Escherichia coli O157:H7 shedding in finishing beef cattle. In Exp. 1, 198 heifers (298 ± 1.1 kg BW) were allocated to 1 of 2 treatments consisting of 1) 200 mg/heifer daily of monensin and 2) 400 mg/ heifer daily of monensin and fed for 151 d. In Exp. 2, 199 steers (430 ± 1.9 kg BW) were stratified by B ...
- Handle:
- 10113/5801819
- DOI:
- 10.2527/jas2017.1528
- https://doi.org/10.2527/jas2017.1528
- Author:
- James E. Wells, et al. ; Elaine D. Berry; Vincent H. Varel; Kristin E. Hales; Norasak Kalchayanand; Show all 5 Authors
- Source:
- Journal of food protection 2017 v.80 no.8 pp. 1317-1327
- ISSN:
- 0362-028X
- Subject:
- Escherichia coli O157; Sorghum (Poaceae); corn; diet; distillers grains; feces; feedlots; pathogens; risk; steers
- Abstract:
- ... Feeding corn wet distillers grains with solubles (WDGS) to cattle can increase the load of Escherichia coli O157:H7 in feces and on hides, but the mechanisms are not fully understood. The objective of these experiments was to examine a role for the persistence of E. coli O157:H7 in the feces and feedlot pen surfaces of cattle fed WDGS. In the first study, feces from steers fed 0, 20, 40, or 60% co ...
- Handle:
- 10113/5834049
- DOI:
- 10.4315/0362-028X.JFP-17-018
- https://doi.org/10.4315/0362-028X.JFP-17-018
- Author:
- James E. Wells, et al. ; Henry A. Paz; Kristin E. Hales; Larry A. Kuehn; Harvey C. Freetly; Elaine D, Berry; Michael D. Flythe; Matthew L. Spangler; Samodha C. Fernando; Show all 9 Authors
- Source:
- Journal of animal science 2018 v.96 no.3 pp. 1045-1058
- ISSN:
- 1525-3163
- Subject:
- Lachnospiraceae; Victivallaceae; bacterial communities; beef cattle; biogeochemical cycles; community structure; diet; feed conversion; heifers; microbiome; models; phenotype; regression analysis; ribosomal RNA; rumen; rumen bacteria; sequence analysis; steers
- Abstract:
- ... The importance of the rumen microbiota on nutrient cycling to the animal is well recognized; however, our understanding of the influence of the rumen microbiome composition on feed efficiency is limited. The rumen microbiomes of two large animal cohorts (125 heifers and 122 steers) were characterized to identify specific bacterial members (operational taxonomic units [OTUs]) associated with feed e ...
- Handle:
- 10113/6472203
- DOI:
- 10.1093/jas/skx081
- PubMed:
- 29617864
- PubMed Central:
- PMC6093515
- https://doi.org/10.1093/jas/skx081
11. Distillers By-Product Cattle Diets Enhance Reduced Sulfur Gas Fluxes from Feedlot Soils and Manures
- Author:
- James E. Wells, et al. ; Daniel N. Miller; Mindy J. Spiehs; Vincent H. Varel; Bryan L. Woodbury; Elaine D. Berry; Show all 6 Authors
- Source:
- Journal of environmental quality 2016 v.45 no.4 pp. 1161-1168
- ISSN:
- 0047-2425
- Subject:
- animal manure management; byproducts; cattle; cattle feeding; cattle feeds; cattle manure; cattle production; corn; diet; distillers grains; emissions; feedlots; nutrient content; phosphorus; soil; soil nutrients; sulfur; surface temperature
- Abstract:
- ... Total reduced sulfur (TRS) emissions from animal feeding operations are a concern with increased feeding of high-sulfur distillers by-products. Three feeding trials were conducted to evaluate feeding wet distillers grain plus solubles (WDGS) on TRS fluxes. Fresh manure was collected three times during Feeding Trial 1 from cattle fed 0, 20, 40, and 60% WDGS. Fluxes of TRS from 40 and 60% WDGS manur ...
- Handle:
- 10113/62789
- DOI:
- 10.2134/jeq2015.09.0480
- https://doi.org/10.2134/jeq2015.09.0480
- Author:
- James E. Wells, et al. ; Phillip R. Myer; Timothy P.L. Smith; Larry A. Kuehn; Harvey C. Freetly; Show all 5 Authors
- Source:
- SpringerPlus 2015 v.4 no. pp. 454
- ISSN:
- 2193-1801
- Subject:
- Butyrivibrio; Oscillospira; Prevotella; Pseudobutyrivibrio; Sphingomonadaceae; average daily gain; body weight; colon; feed conversion; feed intake; fermentation; genes; high-throughput nucleotide sequencing; intestinal microorganisms; microbial communities; nucleotide sequences; ribosomal RNA; rumen microorganisms; species diversity; steers
- Abstract:
- ... Ruminal microbial fermentation plays an essential role in host nutrition, and as a result, the rumen microbiota have been a major focus of research examining bovine feed efficiency. Microbial communities within other sections of the gastrointestinal tract may also be important with regard to feed efficiency, since it is critical to the health and nutrition of the host. The objective of this study ...
- Handle:
- 10113/61315
- DOI:
- 10.1186/s40064-015-1201-6
- PubMed:
- 26322260
- PubMed Central:
- PMC4549364
- https://doi.org/10.1186/s40064-015-1201-6
- Author:
- James E. Wells, et al. ; Phillip R. Myer; Timothy P. L. Smith; Larry A. Kuehn; Harvey C. Freetly; Show all 5 Authors
- Source:
- Plos One 2015 v.10 no.6 pp. -
- ISSN:
- 1932-6203
- Subject:
- Firmicutes; alfalfa hay; cattle feeding; corn; distillers grains; feed conversion; feed intake; feed supplements; genes; high-throughput nucleotide sequencing; humans; liveweight gain; mice; microbiome; nucleotide sequences; ribosomal RNA; rumen bacteria; rumen fluids; steers; urea
- Abstract:
- ... The cattle rumen has a diverse microbial ecosystem that is essential for the host to digest plant material. Extremes in body weight (BW) gain in mice and humans have been associated with different intestinal microbial populations. The objective of this study was to characterize the microbiome of the cattle rumen among steers differing in feed efficiency. Two contemporary groups of steers (n=148 an ...
- Handle:
- 10113/60941
- DOI:
- 10.1371/journal.pone.0129174
- PubMed:
- 26030887
- PubMed Central:
- PMC4451142
- https://doi.org/10.1371/journal.pone.0129174
14. Ammonia, Total Reduced Sulfides, and Greenhouse Gases of Pine Chip and Corn Stover Bedding Packs
- Author:
- James E. Wells, et al. ; Mindy J. Spiehs; Tami M. Brown-Brandl; David B. Parker; Daniel N. Miller; Elaine D. Berry; Show all 6 Authors
- Source:
- Journal of environmental quality 2016 v.45 no.2 pp. 630-637
- ISSN:
- 0047-2425
- Subject:
- Pinus; Zea mays; air quality; air sampling; ammonia; barns; carbon dioxide; cleaning; corn stover; emissions; greenhouse gases; headspace analysis; livestock; methane; nitrous oxide; sulfides; wood chips
- Abstract:
- ... Bedding materials may affect air quality in livestock facilities. Our objective in this study was to compare headspace concentrations of ammonia (NH₃), total reduced sulfides (TRS), carbon dioxide (CO₂), methane (CH₄), and nitrous oxide (N₂O) when pine wood chips (Pinus spp.) and corn stover (Zea mays L.) were mixed in various ratios (0, 10, 20, 30, 40, 60, 80, and 100% pine chips) and used as bed ...
- DOI:
- 10.2134/jeq2015.09.0466
- https://doi.org/10.2134/jeq2015.09.0466
- Author:
- James E. Wells, et al. ; Norasak Kalchayanand; Terrance M. Arthur; Joseph M. Bosilevac; Tommy L. Wheeler; Show all 5 Authors
- Source:
- Journal of food protection 2013 v.76 no.2 pp. 192-199
- ISSN:
- 0362-028X
- Subject:
- Shiga toxin-producing Escherichia coli; agar; bacterial contamination; beef; beef cattle; beta-galactosidase; carbohydrate metabolism; culture media; feces; food contamination; food pathogens; immunomagnetic separation; screening
- Abstract:
- ... Non-O157 Shiga toxin–producing Escherichia coli (STEC) strains are clinically important foodborne pathogens. Unlike E. coli O157:H7, these foodborne pathogens have no unique biochemical characteristics to readily distinguish them from other E. coli strains growing on plating media. In this study, a chromogenic agar medium was developed in order to differentiate among non-O157 STEC strains of serog ...
- Handle:
- 10113/56093
- DOI:
- 10.4315/0362-028X.JFP-12-182
- PubMed:
- 23433363
- https://doi.org/10.4315/0362-028X.JFP-12-182
- Author:
- James E Wells, et al. ; Elaine D Berry; Patricia D Millner; Norasak Kalchayanand; Michael N Guerini; Show all 5 Authors
- Source:
- Journal of food protection 2013 v.76 no.8 pp. 1308-1321
- ISSN:
- 0362-028X
- Subject:
- Campylobacter; Escherichia coli O157; Listeria monocytogenes; Salmonella; agricultural land; animal manures; carbon nitrogen ratio; cattle; composted manure; composting; feedlots; foodborne illness; hay; monitoring; pathogens; risk; screening; straw; temperature; thermophilic bacteria
- Abstract:
- ... Reducing Escherichia coli O157:H7 in livestock manures before application to cropland is critical for reducing the risk of foodborne illness associated with produce. Our objective was to determine the fate of naturally occurring E. coli O157:H7 and other pathogens during minimally managed on-farm bovine manure composting processes. Feedlot pen samples were screened to identify E. coli O157:H7–posi ...
- Handle:
- 10113/57417
- DOI:
- doi:10.4315/0362-028X.JFP-12-364
- https://doi.org/doi:10.4315/0362-028X.JFP-12-364
- Author:
- James E. Wells, et al. ; Elaine D. Berry; James L. Bono; Bryan L. Woodbury; Norasak Kalchayanand; Keri N. Norman; Trevor V. Suslow; Gabriela Lopez-Velasco; Patricia D. Millner; Show all 9 Authors
- Source:
- Applied and environmental microbiology 2015 v.81 no.3 pp. 1101-1110
- ISSN:
- 0099-2240
- Subject:
- Escherichia coli O157; air; airborne transmission; bacterial contamination; beef cattle; cattle manure; cattle production; concentrated animal feeding operations; crop production; crops; feedlots; food contamination; fresh produce; green leafy vegetables; guidelines; pathogens; planting; risk
- Abstract:
- ... The impact of proximity to a beef cattle feedlot on Escherichia coli O157:H7 contamination of leafy greens was examined. In each of 2 years, leafy greens were planted in nine plots located 60, 120, and 180m from a cattle feedlot (3 plots at each distance). Leafy greens (270) and feedlot manure samples (100) were collected six different times from June to September in each year. Both E. coli O157:H ...
- Handle:
- 10113/60859
- DOI:
- 10.1128/AEM.02998-14
- PubMed:
- 25452286
- PubMed Central:
- PMC4292503
- https://doi.org/10.1128/AEM.02998-14
- Author:
- James E. Wells, et al. ; Mindy J. Spiehs; Tami M. Brown-Brandl; David B. Parker; Daniel N. Miller; Elaine D. Berry; Show all 6 Authors
- Source:
- Journal of environmental quality 2013 v.42 no.1 pp. 65-75
- ISSN:
- 0047-2425
- Subject:
- Escherichia coli; Panicum virgatum; air quality; aromatic compounds; beef cattle; corn cobs; corn stover; fatty acids; odor compounds; odors; paper; soybeans; volatile organic compounds; wheat straw; wood chips; wood shavings
- Abstract:
- ... The objectives of this study were to determine the effect of bedding material (corn stover, soybean stover, wheat straw, switchgrass, wood chips, wood shavings, corn cobs, and shredded paper) on concentration of odorous volatile organic compounds (VOC) in bedded pack material and to determine the effect of bedding material on the levels of total Escherichia coli in laboratory-scaled bedded manure ...
- DOI:
- 10.2134/jeq2012.0251
- https://doi.org/10.2134/jeq2012.0251
- Author:
- James E. Wells, et al. ; Vincent H. Varel; Show all 2 Author
- Source:
- Journal of environmental quality 2007 v.36 no.3 pp. 773-779
- ISSN:
- 1537-2537
- Subject:
- thymol; urease inhibitors; coliform bacteria; odor emissions; urea; volatile fatty acids; methane production; pig manure; animal production; waste treatment; Campylobacter; Escherichia coli; air pollution; pollution control; Nebraska
- Abstract:
- ... Pathogens, ammonia, odor, and greenhouse gas emissions are serious environmental concerns associated with swine production. This study was conducted in two manure pits (33 000 L each) to determine the influence of 1.5 or 3.0 g thymol L⁻¹ and 80 mg L⁻¹ urease inhibitor amendments on urea accumulation, coliform bacteria, odor, and methane emission. Each experiment lasted 18 or 19 d, during which tim ...
- Handle:
- 10113/14687
- DOI:
- 10.2134/jeq2006.0394
- PubMed:
- 17412912
- https://doi.org/10.2134/jeq2006.0394
- Author:
- James E. Wells, et al. ; Mindy J. Spiehs; Tami M. Brown-Brandl; David B. Parker; Daniel N. Miller; Jeffrey P. Jaderborg; Alfredo DiCostanzo; Elaine D. Berry; Show all 8 Authors
- Source:
- Journal of environmental quality 2014 v.43 no.4 pp. 1187-1194
- ISSN:
- 0047-2425
- Subject:
- Pinus; air sampling; ammonia; barns; carbon dioxide; cattle manure; corn stover; greenhouse gases; kiln drying; methane; nitrous oxide; sulfides; wood chips
- Abstract:
- ... The objectives of this study were to determine the effect of using corn stover or three different wood-based bedding materials (kiln-dried pine wood chips, dry cedar chips, or green cedar chips) on airborne concentrations of NH₃, total reduced sulfides (TRS), CO₂, CH₄, and N₂O above lab-scaled bedded manure packs. Four bedded packs of each bedding material were maintained for two 42-d periods. Air ...
- DOI:
- 10.2134/jeq2013.05.0164
- https://doi.org/10.2134/jeq2013.05.0164