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- Author:
- Jin, Feng-Jie, et al. ; Han, Pei; Zhuang, Miao; Zhang, Zhi-Min; Jin, Long; Koyama, Yasuji; Show all 6 Authors
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.1 pp. 319-332
- ISSN:
- 0175-7598
- Subject:
- Aspergillus oryzae; alpha-glucosidase; alpha-mannosidase; beta-galactosidase; carbohydrate metabolism; carbon metabolism; genes; liquids; mammals; manufacturing; microorganisms; phenotype; protein degradation; protein synthesis; proteome; proteomics; reverse transcriptase polymerase chain reaction; transcription factors
- Abstract:
- ... The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes, such as rapid protein degradation and cell lysis in dextrin-polypeptone-yeast e ...
- DOI:
- 10.1007/s00253-017-8588-7
-
https://dx.doi.org/10.1007/s00253-017-8588-7
- Author:
- Jin, Feng-Jie, et al. ; Jin, Long; Wu, Xuewen; Ko, So-Ra; Li, Taihua; Ahn, Chi-Yong; Oh, Hee-Mock; Lee, Hyung-Gwan; Show all 8 Authors
- Source:
- Antonie van Leeuwenhoek 2018 v.111 no.12 pp. 2283-2292
- ISSN:
- 0003-6072
- Subject:
- Hymenobacter; fatty acids; genes; grasses; multilocus sequence typing; new species; nucleic acid hybridization; nucleotide sequences; phenotype; phosphatidylethanolamines; phylogeny; quinones; ribosomal DNA; ribosomal RNA; sequence homology; soil; Korean Peninsula
- Abstract:
- ... A polyphasic taxonomic study was carried out on strains PB105ᵀ and PB108 isolated from a grass soil in Korea. The cells of the strains were Gram-stain negative, non-spore-forming, non-motile, and rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of these strains with Bacteroidetes, which showed high pairwise sequence similarities with Hymenobacter algoricola VUG-A23 ...
- DOI:
- 10.1007/s10482-018-1119-4
-
http://dx.doi.org/10.1007/s10482-018-1119-4
- Author:
- Jin, Feng-Jie, et al. ; Ren, Ting-Ting; Jin, Chun-Zhi; Li, Taihua; Kim, Chang-Jin; Oh, Hee-Mock; Lee, Hyung-Gwan; Jin, Long; Show all 8 Authors
- Source:
- The journal of microbiology 2018 v.56 no.7 pp. 467-471
- ISSN:
- 1225-8873
- Subject:
- DNA; Sphingobacteriia; bacteria; eutrophication; fatty acids; freshwater; genome; menaquinones; nucleic acid hybridization; nucleotide sequences; phenotype; phosphatidylethanolamines; ribosomal RNA; sediments; South Korea
- Abstract:
- ... A Gram-stain-positive, aerobic, non-motile, non-spore-forming, and rod-shaped bacterium, designated strain CHu64-6-1ᵀ, was isolated from a 67-cm-long sediment core collected from the Daechung Reservoir at a water depth of 17-m in Daejeon, Republic of Korea. Comparative 16S rRNA gene sequence studies placed the new isolate in the class Sphingobacteriia, and the isolate is notably most closely relat ...
- DOI:
- 10.1007/s12275-018-7567-8
-
http://dx.doi.org/10.1007/s12275-018-7567-8
- Author:
- Jin, Feng-Jie, et al. ; Katayama, Takuya; Maruyama, Jun-ichi; Kitamoto, Katsuhiko; Show all 4 Authors
- Source:
- Applied microbiology and biotechnology 2016 v.100 no.21 pp. 9163-9174
- ISSN:
- 0175-7598
- Subject:
- Aspergillus oryzae; fungi; genes; genomics; heterologous gene expression; high-throughput nucleotide sequencing; human diseases; humans; lysozyme; mutagenesis; mutants; proteins; screening; sequence deletion
- Abstract:
- ... Genomic mapping of mutations using next-generation sequencing technologies has facilitated the identification of genes contributing to fundamental biological processes, including human diseases. However, few studies have used this approach to identify mutations contributing to heterologous protein production in industrial strains of filamentous fungi, such as Aspergillus oryzae. In a screening of ...
- DOI:
- 10.1007/s00253-016-7714-2
-
http://dx.doi.org/10.1007/s00253-016-7714-2
- Author:
- Jin, Feng Jie, et al. ; Nakamura, Hidetoshi; Kikuma, Takashi; Maruyama, Jun-ichi; Kitamoto, Katsuhiko; Show all 5 Authors
- Source:
- Journal of bioscience and bioengineering 2016 v.121 no.4 pp. 365-371
- ISSN:
- 1389-1723
- Subject:
- Aspergillus oryzae; Saccharomyces cerevisiae; asexual reproduction; conidia; conidiation; gene overexpression; genes; heat stress; phenotype; sclerotia; stress tolerance; yeasts
- Abstract:
- ... The serine–threonine kinase Rim15p is a master regulator of stress signaling and is required for stress tolerance and sexual sporulation in the yeast Saccharomyces cerevisiae. However, in filamentous fungi that reproduce asexually via conidiation, the physiological function of Rim15p homologs has not been extensively analyzed. Here, we functionally characterized the protein homolog of Rim15p in th ...
- DOI:
- 10.1016/j.jbiosc.2015.08.011
-
https://dx.doi.org/10.1016/j.jbiosc.2015.08.011
- Author:
- Jin, Feng Jie, et al. ; Wada, Ryuta; Koyama, Yasuji; Maruyama, Jun-ichi; Kitamoto, Katsuhiko; Show all 5 Authors
- Source:
- Applied microbiology and biotechnology 2014 v.98 no.1 pp. 325-334
- ISSN:
- 0175-7598
- Subject:
- Aspergillus oryzae; Neurospora crassa; adenine; auxotrophs; fluorescence; fungi; gene overexpression; genes; heterokaryon; hyphae; parasexual cycle; proteins; sclerotia; sexual reproduction; transcription factors; uracil; uridine
- Abstract:
- ... Heterokaryon formation by hyphal fusion occurs during a sexual/parasexual cycle in filamentous fungi, and therefore, it is biotechnologically important for crossbreeding. In the industrial filamentous fungus Aspergillus oryzae, a parasexual cycle has been reported, and it was recently suggested that sexual reproduction should be possible. However, as A. oryzae enters into hyphal fusion with a much ...
- DOI:
- 10.1007/s00253-013-5314-y
- PubMed:
- 24201891
-
http://dx.doi.org/10.1007/s00253-013-5314-y
- Author:
- Jin, Feng Jie, et al. ; Hara, Seiichi; Takahashi, Tadashi; Koyama, Yasuji; Show all 4 Authors
- Source:
- Molecular genetics and genomics 2012 v.287 no.2 pp. 177-187
- ISSN:
- 1617-4615
- Subject:
- Aspergillus oryzae; DNA; benomyl; chromosomes; comparative genomic hybridization; diploidy; flow cytometry; genes; haploidy; mutants; polymerase chain reaction; protoplast fusion; pulsed-field gel electrophoresis
- Abstract:
- ... Our goal in this work was to develop a method to minimize the chromosomes of Aspergillus oryzae, to arrive at a deeper understanding of essential gene functions that will help create more efficient industrially useful strains. In a previous study, we successfully constructed a highly reduced chromosome 7 using multiple large-scale chromosomal deletions (Jin et al. in Mol Genet Genomics 283:1–12, 2 ...
- DOI:
- 10.1007/s00438-011-0669-1
- PubMed:
- 22207035
-
http://dx.doi.org/10.1007/s00438-011-0669-1
- Author:
- Jin, Feng Jie, et al. ; Nishida, Michiyo; Hara, Seiichi; Koyama, Yasuji; Show all 4 Authors
- Source:
- Fungal genetics and biology 2011 v.48 no.12 pp. 1108-1115
- ISSN:
- 1087-1845
- Subject:
- Aspergillus oryzae; basic helix-loop-helix transcription factors; conidia; conidiation; gene overexpression; genes; phenotype; proteins; transcription (genetics); yeasts
- Abstract:
- ... The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes. HLH proteins form homo- and/or heterodimers with other HLH proteins and bind to E-box motifs. The regulation and functions of these proteins can be complex due to their bifunctional roles as activators and repressors of gene transcription. In this study, we isolated and characte ...
- DOI:
- 10.1016/j.fgb.2011.10.001
- PubMed:
- 22008745
-
http://dx.doi.org/10.1016/j.fgb.2011.10.001
- Author:
- Jin, Feng Jie, et al. ; Takahashi, Tadashi; Utsushikawa, Michiyo; Furukido, Toshi; Nishida, Michiyo; Ogawa, Masahiro; Tokuoka, Masahumi; Koyama, Yasuji; Show all 8 Authors
- Source:
- Molecular genetics and genomics 2010 v.283 no.1 pp. 1-12
- ISSN:
- 1617-4615
- Subject:
- Aspergillus oryzae; chromosomes; gene expression; gene expression regulation; genes; hyphae; metabolism; microarray technology; mutants
- Abstract:
- ... We aim to create an Aspergillus oryzae mutant with a highly reduced chromosome, but better growth, by eliminating the nonessential regions coding various dispensable functions for its better industrial use. In our previous study, we successfully determined the outline of essential and nonessential regions by constructing a series of large chromosomal deletions in A. oryzae chromosome 7. Based on t ...
- DOI:
- 10.1007/s00438-009-0494-y
- PubMed:
- 19855999
-
http://dx.doi.org/10.1007/s00438-009-0494-y
- Author:
- Jin, Feng Jie, et al. ; Watanabe, Taisuke; Juvvadi, Praveen Rao; Maruyama, Jun-ichi; Arioka, Manabu; Kitamoto, Katsuhiko; Show all 6 Authors
- Source:
- Applied microbiology and biotechnology 2007 v.76 no.5 pp. 1059-1068
- ISSN:
- 0175-7598
- Subject:
- Aspergillus oryzae; alpha-amylase; fungi; gene targeting; genes; heterologous gene expression; humans; lysozyme; models; proteinases
- Abstract:
- ... In this study, we investigated the effects of proteinase gene disruption on heterologous protein production by Aspergillus oryzae. The human lysozyme (HLY) was selected for recombinant production as a model for the heterologous protein. A tandem HLY construct fused with α-amylase (AmyB) was expressed by A. oryzae in which the Kex2 cleavage site was inserted at the upstream of HLY. HLY was successf ...
- DOI:
- 10.1007/s00253-007-1088-4
- PubMed:
- 17622525
-
http://dx.doi.org/10.1007/s00253-007-1088-4