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1. Comparative proteomic analysis: SclR is importantly involved in carbohydrate metabolism in Aspergillus oryzae

Author:
Jin, Feng-Jie,   et al.   ; Han, Pei; Zhuang, Miao; Zhang, Zhi-Min; Jin, Long; Koyama, Yasuji;     Show all 6 Authors
Source:
Applied microbiology and biotechnology 2018 v.102 no.1 pp. 319-332
ISSN:
0175-7598
Subject:
Aspergillus oryzae; alpha-glucosidase; alpha-mannosidase; beta-galactosidase; carbohydrate metabolism; carbon metabolism; genes; liquids; mammals; manufacturing; microorganisms; phenotype; protein degradation; protein synthesis; proteome; proteomics; reverse transcriptase polymerase chain reaction; transcription factors
Abstract:
... The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes, such as rapid protein degradation and cell lysis in dextrin-polypeptone-yeast e ...
DOI:
10.1007/s00253-017-8588-7

2. Description of Hymenobacter daejeonensis sp. nov., isolated from grass soil, based on multilocus sequence analysis of the 16S rRNA gene, gyrB and tuf genes

Author:
Jin, Feng-Jie,   et al.   ; Jin, Long; Wu, Xuewen; Ko, So-Ra; Li, Taihua; Ahn, Chi-Yong; Oh, Hee-Mock; Lee, Hyung-Gwan;     Show all 8 Authors
Source:
Antonie van Leeuwenhoek 2018 v.111 no.12 pp. 2283-2292
ISSN:
0003-6072
Subject:
Hymenobacter; fatty acids; genes; grasses; multilocus sequence typing; new species; nucleic acid hybridization; nucleotide sequences; phenotype; phosphatidylethanolamines; phylogeny; quinones; ribosomal DNA; ribosomal RNA; sequence homology; soil; Korean Peninsula
Abstract:
... A polyphasic taxonomic study was carried out on strains PB105ᵀ and PB108 isolated from a grass soil in Korea. The cells of the strains were Gram-stain negative, non-spore-forming, non-motile, and rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of these strains with Bacteroidetes, which showed high pairwise sequence similarities with Hymenobacter algoricola VUG-A23 ...
DOI:
10.1007/s10482-018-1119-4

3. Flavihumibacter profundi sp. nov., isolated from eutrophic freshwater sediment

Author:
Jin, Feng-Jie,   et al.   ; Ren, Ting-Ting; Jin, Chun-Zhi; Li, Taihua; Kim, Chang-Jin; Oh, Hee-Mock; Lee, Hyung-Gwan; Jin, Long;     Show all 8 Authors
Source:
The journal of microbiology 2018 v.56 no.7 pp. 467-471
ISSN:
1225-8873
Subject:
DNA; Sphingobacteriia; bacteria; eutrophication; fatty acids; freshwater; genome; menaquinones; nucleic acid hybridization; nucleotide sequences; phenotype; phosphatidylethanolamines; ribosomal RNA; sediments; South Korea
Abstract:
... A Gram-stain-positive, aerobic, non-motile, non-spore-forming, and rod-shaped bacterium, designated strain CHu64-6-1ᵀ, was isolated from a 67-cm-long sediment core collected from the Daechung Reservoir at a water depth of 17-m in Daejeon, Republic of Korea. Comparative 16S rRNA gene sequence studies placed the new isolate in the class Sphingobacteriia, and the isolate is notably most closely relat ...
DOI:
10.1007/s12275-018-7567-8

4. Comparative genomic analysis identified a mutation related to enhanced heterologous protein production in the filamentous fungus Aspergillus oryzae

Author:
Jin, Feng-Jie,   et al.   ; Katayama, Takuya; Maruyama, Jun-ichi; Kitamoto, Katsuhiko;     Show all 4 Authors
Source:
Applied microbiology and biotechnology 2016 v.100 no.21 pp. 9163-9174
ISSN:
0175-7598
Subject:
Aspergillus oryzae; fungi; genes; genomics; heterologous gene expression; high-throughput nucleotide sequencing; human diseases; humans; lysozyme; mutagenesis; mutants; proteins; screening; sequence deletion
Abstract:
... Genomic mapping of mutations using next-generation sequencing technologies has facilitated the identification of genes contributing to fundamental biological processes, including human diseases. However, few studies have used this approach to identify mutations contributing to heterologous protein production in industrial strains of filamentous fungi, such as Aspergillus oryzae. In a screening of ...
DOI:
10.1007/s00253-016-7714-2

5. AoRim15 is involved in conidial stress tolerance, conidiation and sclerotia formation in the filamentous fungus Aspergillus oryzae

Author:
Jin, Feng Jie,   et al.   ; Nakamura, Hidetoshi; Kikuma, Takashi; Maruyama, Jun-ichi; Kitamoto, Katsuhiko;     Show all 5 Authors
Source:
Journal of bioscience and bioengineering 2016 v.121 no.4 pp. 365-371
ISSN:
1389-1723
Subject:
Aspergillus oryzae; Saccharomyces cerevisiae; asexual reproduction; conidia; conidiation; gene overexpression; genes; heat stress; phenotype; sclerotia; stress tolerance; yeasts
Abstract:
... The serine–threonine kinase Rim15p is a master regulator of stress signaling and is required for stress tolerance and sexual sporulation in the yeast Saccharomyces cerevisiae. However, in filamentous fungi that reproduce asexually via conidiation, the physiological function of Rim15p homologs has not been extensively analyzed. Here, we functionally characterized the protein homolog of Rim15p in th ...
DOI:
10.1016/j.jbiosc.2015.08.011

6. Efficient formation of heterokaryotic sclerotia in the filamentous fungus Aspergillus oryzae

Author:
Jin, Feng Jie,   et al.   ; Wada, Ryuta; Koyama, Yasuji; Maruyama, Jun-ichi; Kitamoto, Katsuhiko;     Show all 5 Authors
Source:
Applied microbiology and biotechnology 2014 v.98 no.1 pp. 325-334
ISSN:
0175-7598
Subject:
Aspergillus oryzae; Neurospora crassa; adenine; auxotrophs; fluorescence; fungi; gene overexpression; genes; heterokaryon; hyphae; parasexual cycle; proteins; sclerotia; sexual reproduction; transcription factors; uracil; uridine
Abstract:
... Heterokaryon formation by hyphal fusion occurs during a sexual/parasexual cycle in filamentous fungi, and therefore, it is biotechnologically important for crossbreeding. In the industrial filamentous fungus Aspergillus oryzae, a parasexual cycle has been reported, and it was recently suggested that sexual reproduction should be possible. However, as A. oryzae enters into hyphal fusion with a much ...
DOI:
10.1007/s00253-013-5314-y
PubMed:
24201891

7. A further study on chromosome minimization by protoplast fusion in Aspergillus oryzae

Author:
Jin, Feng Jie,   et al.   ; Hara, Seiichi; Takahashi, Tadashi; Koyama, Yasuji;     Show all 4 Authors
Source:
Molecular genetics and genomics 2012 v.287 no.2 pp. 177-187
ISSN:
1617-4615
Subject:
Aspergillus oryzae; DNA; benomyl; chromosomes; comparative genomic hybridization; diploidy; flow cytometry; genes; haploidy; mutants; polymerase chain reaction; protoplast fusion; pulsed-field gel electrophoresis
Abstract:
... Our goal in this work was to develop a method to minimize the chromosomes of Aspergillus oryzae, to arrive at a deeper understanding of essential gene functions that will help create more efficient industrially useful strains. In a previous study, we successfully constructed a highly reduced chromosome 7 using multiple large-scale chromosomal deletions (Jin et al. in Mol Genet Genomics 283:1–12, 2 ...
DOI:
10.1007/s00438-011-0669-1
PubMed:
22207035

8. Identification and characterization of a putative basic helix-loop-helix transcription factor involved in the early stage of conidiophore development in Aspergillus oryzae

Author:
Jin, Feng Jie,   et al.   ; Nishida, Michiyo; Hara, Seiichi; Koyama, Yasuji;     Show all 4 Authors
Source:
Fungal genetics and biology 2011 v.48 no.12 pp. 1108-1115
ISSN:
1087-1845
Subject:
Aspergillus oryzae; basic helix-loop-helix transcription factors; conidia; conidiation; gene overexpression; genes; phenotype; proteins; transcription (genetics); yeasts
Abstract:
... The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes. HLH proteins form homo- and/or heterodimers with other HLH proteins and bind to E-box motifs. The regulation and functions of these proteins can be complex due to their bifunctional roles as activators and repressors of gene transcription. In this study, we isolated and characte ...
DOI:
10.1016/j.fgb.2011.10.001
PubMed:
22008745

9. Nonhomologous end-joining deficiency allows large chromosomal deletions to be produced by replacement-type recombination in Aspergillus oryzae

Author:
Jin, Feng Jie,   et al.   ; Takahashi, Tadashi; Koyama, Yasuji;     Show all 3 Authors
Source:
Fungal genetics and biology 2009 v.46 no.11 pp. 815-824
ISSN:
1087-1845
Subject:
Aspergillus oryzae; microbial genetics; genetic recombination; chromosomes; gene targeting
DOI:
10.1016/j.fgb.2009.07.009
PubMed:
19654050

10. Identification of a Basic Helix-Loop-Helix-Type Transcription Regulator Gene in Aspergillus oryzae by Systematically Deleting Large Chromosomal Segments

Author:
Jin, Feng Jie,   et al.   ; Takahashi, Tadashi; Machida, Masayuki; Koyama, Yasuji;     Show all 4 Authors
Source:
Applied and environmental microbiology 2009 v.75 no.18 pp. 5943-5951
ISSN:
0099-2240
Subject:
Aspergillus oryzae; DNA; basic helix-loop-helix transcription factors; binding proteins; chromosomes; engineering; industrial applications; mutants; phenotype; prediction; regulator genes
Abstract:
... We previously developed two methods (loop-out and replacement-type recombination) for generating large-scale chromosomal deletions that can be applied to more effective chromosomal engineering in Aspergillus oryzae. In this study, the replacement-type method is used to systematically delete large chromosomal DNA segments to identify essential and nonessential regions in chromosome 7 (2.93 Mb), whi ...
DOI:
10.1128/AEM.00975-09

11. Genetic analysis of conidiation regulatory pathways in koji-mold Aspergillus oryzae

Author:
Jin, Feng Jie,   et al.   ; Ogawa, Masahiro; Tokuoka, Masafumi; Takahashi, Tadashi; Koyama, Yasuji;     Show all 5 Authors
Source:
Fungal genetics and biology 2010 v.47 no.1 pp. 10-18
ISSN:
1087-1845
Subject:
Aspergillus oryzae; fermented foods; koji; sporulation; conidia; mutants; phenotype; microbial genetics; gene expression; messenger RNA; nucleotide sequences
DOI:
10.1016/j.fgb.2009.10.004
PubMed:
19850144

12. A trial of minimization of chromosome 7 in Aspergillus oryzae by multiple chromosomal deletions

Author:
Jin, Feng Jie,   et al.   ; Takahashi, Tadashi; Utsushikawa, Michiyo; Furukido, Toshi; Nishida, Michiyo; Ogawa, Masahiro; Tokuoka, Masahumi; Koyama, Yasuji;     Show all 8 Authors
Source:
Molecular genetics and genomics 2010 v.283 no.1 pp. 1-12
ISSN:
1617-4615
Subject:
Aspergillus oryzae; chromosomes; gene expression; gene expression regulation; genes; hyphae; metabolism; microarray technology; mutants
Abstract:
... We aim to create an Aspergillus oryzae mutant with a highly reduced chromosome, but better growth, by eliminating the nonessential regions coding various dispensable functions for its better industrial use. In our previous study, we successfully determined the outline of essential and nonessential regions by constructing a series of large chromosomal deletions in A. oryzae chromosome 7. Based on t ...
DOI:
10.1007/s00438-009-0494-y
PubMed:
19855999

13. Generation of Large Chromosomal Deletions in Koji Molds Aspergillus oryzae and Aspergillus sojae via a Loop-Out Recombination

Author:
Jin, Feng Jie,   et al.   ; Takahashi, Tadashi; Sunagawa, Misao; Machida, Masayuki; Koyama, Yasuji;     Show all 5 Authors
Source:
Applied and environmental microbiology 2008 v.74 no.24 pp. 7684-7693
ISSN:
0099-2240
Subject:
Aspergillus oryzae; aflatoxins; breeding value; engineering; fungi; homologous recombination; koji; multigene family
Abstract:
... We established a technique for efficiently generating large chromosomal deletions in the koji molds Aspergillus oryzae and A. sojae by using a ku70-deficient strain and a bidirectional marker. The approach allowed deletion of 200-kb and 100-kb sections of A. oryzae and A. sojae, respectively. The deleted regions contained putative aflatoxin biosynthetic gene clusters. The large genomic deletions g ...
DOI:
10.1128/AEM.00692-08

14. Double disruption of the proteinase genes, tppA and pepE, increases the production level of human lysozyme by Aspergillus oryzae

Author:
Jin, Feng Jie,   et al.   ; Watanabe, Taisuke; Juvvadi, Praveen Rao; Maruyama, Jun-ichi; Arioka, Manabu; Kitamoto, Katsuhiko;     Show all 6 Authors
Source:
Applied microbiology and biotechnology 2007 v.76 no.5 pp. 1059-1068
ISSN:
0175-7598
Subject:
Aspergillus oryzae; alpha-amylase; fungi; gene targeting; genes; heterologous gene expression; humans; lysozyme; models; proteinases
Abstract:
... In this study, we investigated the effects of proteinase gene disruption on heterologous protein production by Aspergillus oryzae. The human lysozyme (HLY) was selected for recombinant production as a model for the heterologous protein. A tandem HLY construct fused with α-amylase (AmyB) was expressed by A. oryzae in which the Kex2 cleavage site was inserted at the upstream of HLY. HLY was successf ...
DOI:
10.1007/s00253-007-1088-4
PubMed:
17622525