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- Author:
- John D. Lippolis, et al. ; Ellie J. Putz; Timothy A. Reinhardt; Eduardo Casas; Wanda J. Weber; Brian A. Crooker; Show all 6 Authors
- Source:
- Journal of dairy science 2022 v.105 no.6 pp. 5435-5448
- ISSN:
- 0022-0302
- Subject:
- Escherichia coli; Holstein; blood serum; body temperature; gene expression regulation; genotype; herds; immune response; innate immunity; interleukin-6; mammary glands; milk; milk yield; neutrophils; somatic cell count; transcriptome; whey; Minnesota
- Abstract:
- ... Selective breeding of US dairy cows since the mid-1960s has contributed to remarkable gains in milk yield per cow. This increased milk yield has been associated with an increase in health issues. Since 1964, the University of Minnesota has selectively bred a Holstein herd to maintain genetically static, unselected Holsteins (UH). Comparison of these UH cows with contemporary Holsteins (CH) has dem ...
- Handle:
- 10113/7752301
- DOI:
- 10.3168/jds.2021-21166
- https://doi.org/10.3168/jds.2021-21166
- Author:
- John D. Lippolis, et al. ; Timothy A. Reinhardt; Show all 2 Author
- Source:
- Data in Brief 2020 v.31 no. pp. 105954
- ISSN:
- 2352-3409
- Subject:
- cattle; data collection; lactation; liquid chromatography; mammary glands; milk; proteome; secretion; tandem mass spectrometry
- Abstract:
- ... This dataset is a label-free quantitation of proteins milk and dry secretions from the end of lactation through day 21 of the dry period using liquid chromatography with tandem mass spectrometry (LC-MS/MS). The data supplied in this article supports the accompanying publication entitled “Characterization of bovine mammary gland dry secretions and their proteome from the end of lactation through da ...
- DOI:
- 10.1016/j.dib.2020.105954
- CHORUS:
- 10.1016/j.dib.2020.105954
- https://doi.org/10.1016/j.dib.2020.105954
- Author:
- John D. Lippolis, et al. ; Amber A. Brink; Wanda J. Weber; John B. Cole; Sandra M. Godden; Anthony Seykora; Brian A. Crooker; Show all 7 Authors
- Source:
- Veterinary immunology and immunopathology 2022 v.251 pp. 110463
- ISSN:
- 0165-2427
- Subject:
- Holstein; energy balance; genotype; immunopathology; innate immunity; interleukin-6; lactation; lipopolysaccharides; lipoteichoic acids; milk; pathogen-associated molecular patterns
- Abstract:
- ... Effects of Holstein genotype on innate immune response were assessed with ex-vivo lipopolysaccharide (LPS) and lipoteichoic acid (LTA) stimulation of whole blood from unselected (UH, n = 10) and contemporary (CH, n = 11) Holsteins that differ in production by more than 4,500 kg/lactation. Blood was collected at −14, 7, 28, and 49 days in milk (DIM), mixed with a pathogen-associated molecular patte ...
- DOI:
- 10.1016/j.vetimm.2022.110463
- https://doi.org/10.1016/j.vetimm.2022.110463
- Author:
- John D. Lippolis, et al. ; Ellie J. Putz; Luis G.V. Fernandes; Sathesh K. Sivasankaran; Darrell O. Bayles; David P. Alt; Jarlath E. Nally; Show all 7 Authors
- Source:
- Journal of proteomics 2022 v.262 pp. 104602
- ISSN:
- 1874-3919
- Subject:
- Leptospira borgpetersenii subsp. hardjo; body temperature; cattle; cold; cross immunity; death; gene expression; leptospirosis; people; protein synthesis; proteome; proteomics; serotypes; slaughterhouses; transcriptome; vaccine development; vaccines; wildlife; zoonoses
- Abstract:
- ... Leptospirosis is a global zoonotic disease affecting humans and livestock species. Bacterin vaccines lack cross protection between serogroups, and include multiple serovars propagated at 29 °C. Recent work demonstrated substantial variation in the transcriptome of identical species and serovars of Leptospira. Here, substantial differences in protein abundance profiles were identified in Leptospira ...
- Handle:
- 10113/7767080
- DOI:
- 10.1016/j.jprot.2022.104602
- CHORUS:
- 10.1016/j.jprot.2022.104602
- https://doi.org/10.1016/j.jprot.2022.104602
- Author:
- John D. Lippolis, et al. ; Timothy A. Reinhardt; Show all 2 Author
- Source:
- Journal of proteomics 2020 v.223 pp. 103831
- ISSN:
- 1874-3919
- Subject:
- antimicrobial proteins; bacterial growth; coliform bacteria; dairy cows; gene expression regulation; gene ontology; immune response; lactation; lactoferrin; mammary glands; mass spectrometry; mastitis; milk; pathogens; protein content; proteome; secretion; whey protein
- Abstract:
- ... The dry period in dairy cows is critical to mammary health. We used label-free mass spectroscopy to quantify whey proteins from milk/dry secretions collected on days 0, 3,10, and 21 of the dry period. The number of proteins identified was 776. The number of proteins upregulated at one or more times in the dry period was 109. The most significant enriched Gene Ontology (GO) terms for the upregulate ...
- Handle:
- 10113/6963593
- DOI:
- 10.1016/j.jprot.2020.103831
- https://doi.org/10.1016/j.jprot.2020.103831
- Author:
- John D. Lippolis, et al. ; Ellie J. Putz; Mitchell V. Palmer; Hao Ma; Eduardo Casas; Timothy A. Reinhardt; Show all 6 Authors
- Source:
- BMC veterinary research 2020 v.16 no.1 pp. 336
- ISSN:
- 1746-6148
- Subject:
- Gram-positive bacteria; Holstein; Staphylococcus aureus; abscess; antibiotics; calving; case studies; connective tissues; dairy cows; epithelium; granulocyte colony-stimulating factor; granuloma; infection; lactation; mastitis; mechanics; milk; myeloperoxidase; necropsy; neutrophils; signs and symptoms (animals and humans); somatic cell count; traps; veterinary medicine
- Abstract:
- ... BACKGROUND: Mastitis is the most common health concern plaguing the modern dairy cow and costs dairy producers estimates of two billion dollars annually. Staphylococcus aureus infections are prevalent, displaying varied disease presentation and markedly low cure rates. Neutrophils are considered the first line of defense against mastitis causing bacteria and are frequently targeted in the developm ...
- Handle:
- 10113/7110991
- DOI:
- 10.1186/s12917-020-02528-8
- https://doi.org/10.1186/s12917-020-02528-8
- Author:
- John D. Lippolis, et al. ; André M. Almeida; Syed Azmal Ali; Fabrizio Ceciliani; P. David Eckersall; Lorenzo E. Hernández-Castellano; Rongwei Han; Jaka J. Hodnik; Shalini Jaswal; Mark McLaughlin; Ingrid Miller; Ashok Kumar Mohanty; Vladimir Mrljak; Jarlath E. Nally; Paolo Nanni; Jeffrey E. Plowman; Mirele D. Poleti; David M. Ribeiro; Pedro Rodrigues; Bernd Roschitzki; Ralph Schlapbach; Jože Starič; Yongxin Yang; Maya Zachut; Show all 24 Authors
- Source:
- Journal of proteomics 2021 v.241 pp. 104220
- ISSN:
- 1874-3919
- Subject:
- animal production; climate change; databases; domestic animals; food availability; food supply chain; international cooperation; metabolomics; proteomics; transcriptomics
- Abstract:
- ... Animal production and health are of significant economic importance, particularly regarding the world food supply. Animal and veterinary sciences have evolved immensely in the past six decades, particularly in genetics, nutrition, housing, management and health. To address major challenges such as those posed by climate change or metabolic disorders, it is of utmost importance to use state-of-the- ...
- Handle:
- 10113/7341386
- DOI:
- 10.1016/j.jprot.2021.104220
- https://doi.org/10.1016/j.jprot.2021.104220
- Author:
- John D. Lippolis, et al. ; Devin B. Holman; Brian W. Brunelle; Tyler C. Thacker; Bradley L. Bearson; Timothy A. Reinhardt; Randy E. Sacco; Thomas A. Casey; Show all 8 Authors
- Source:
- Infection and immunity 2018 v.86 no.1 pp. e00566-17
- ISSN:
- 0019-9567
- Subject:
- DNA; Escherichia coli; bacteria; bacterial motility; bovine mastitis; chronic diseases; complement; dairy cattle; gene expression; genomics; operon; phenotype; sequence analysis; transcriptomics
- Abstract:
- ... Escherichia coli is a leading cause of bacterial mastitis in dairy cattle. It is most often transient in nature, causing an infection that lasts 2 to 3 days. However, E. coli has been shown to cause a persistent infection in a minority of cases. Mechanisms that allow for a persistent E. coli infection are not fully understood. The goal of this work was to determine differences between E. coli stra ...
- Handle:
- 10113/6564235
- DOI:
- 10.1128/IAI.00566-17
- PubMed:
- 29061709
- PubMed Central:
- PMC5736815
- https://doi.org/10.1128/IAI.00566-17
- Author:
- John D. Lippolis, et al. ; Indira T. Kudva; Thaddeus B. Stanton; Show all 3 Authors
- Source:
- BMC Microbiology 2014 v.14 no.48 pp. -
- ISSN:
- 1471-2180
- Subject:
- Escherichia coli O157; anaerobic conditions; cattle; cell structures; diet; fatty acid composition; gene expression; in vitro studies; pH; protein content; protein synthesis; proteins; proteome; proteomics; rumen fluids; rumen microorganisms; virulence; volatile fatty acids
- Abstract:
- ... Background: To obtain insights into Escherichia coli O157:H7 (O157) survival mechanisms in the bovine rumen, we defined the growth characteristics and proteome of O157 cultured in rumen fluid (RF; pH 6.0-7.2 and low volatile fatty acid content) obtained from rumen-fistulated cattle fed low protein content "maintenance diet" under diverse in vitro conditions. Results: Bottom-up proteomics (LC-MS/MS ...
- Handle:
- 10113/59367
- DOI:
- 10.1186/1471-2180-14-48
- PubMed:
- 24559513
- PubMed Central:
- PMC3936929
- https://doi.org/10.1186/1471-2180-14-48
- Author:
- John D. Lippolis, et al. ; Timothy A. Reinhardt; Randy E. Sacco; Show all 3 Authors
- Source:
- Biochemical and Biophysical Research Communications 2014 v.445 no.2 pp. 417-421
- ISSN:
- 0006-291X
- Subject:
- Golgi apparatus; adenosinetriphosphatase; antiporters; calcium; cells; exhibitions; knockout mutants; lactation; mammary glands; milk; plasma membrane; pregnancy
- Abstract:
- ... Plasma membrane Ca(2+)-ATPase 2 (PMCA2) knockout mice showed that ~ 60 % of calcium in milk is transported across the mammary cells apical membrane by PMCA2. The remaining milk calcium is thought to arrive via the secretory pathway through the actions of secretory pathway Ca(2+)-ATPase’s 1 and/or 2 (SPCA1 and 2). However, another secretory pathway calcium transporter was recently described. The qu ...
- Handle:
- 10113/61347
- DOI:
- 10.1016/j.bbrc.2014.02.020
- https://doi.org/10.1016/j.bbrc.2014.02.020
- Author:
- John D. Lippolis, et al. ; Randal T. Capsel; Charles O. Thoen; Timothy A. Reinhardt; Renee Olsen; Judith R. Stabel; John P. Bannantine; Show all 7 Authors
- Source:
- Plos One 2016 v.11 no.5 pp. e0154685
- ISSN:
- 1932-6203
- Subject:
- Escherichia coli; Mycobacterium avium subsp. paratuberculosis; antibodies; antigens; autoclaving; blood; erythema; filtrates; filtration; guinea pigs; immunoblotting; interferon-gamma; mass spectrometry; polyacrylamide gel electrophoresis; recombinant proteins
- Abstract:
- ... Mycobacterium avium subsp. paratuberculosis (MAP) purified protein derivatives (PPDs) are immunologic reagents prepared from cultured filtrates of the type strain. Traditional production consists of floating culture incubation at 37°C, organism inactivation by autoclaving, coarse filtration, and protein precipitation. Three traditional production PPDs were used in this study including lot 9801, wh ...
- Handle:
- 10113/62940
- DOI:
- 10.1371/journal.pone.0154685
- PubMed:
- 27136199
- PubMed Central:
- PMC4852940
- CHORUS:
- 10.1371/journal.pone.0154685
- https://doi.org/10.1371/journal.pone.0154685
- Author:
- John D. Lippolis, et al. ; Brian W. Brunelle; Timothy A. Reinhardt; Randy E. Sacco; Tyler C. Thacker; Torey P. Looft; Thomas A. Casey; Show all 7 Authors
- Source:
- mSystems 2016 v.1 no.4 pp. -
- ISSN:
- 2379-5077
- Subject:
- Escherichia coli; bacteria; bacterial motility; chelation; chronic diseases; culture media; dairy cattle; gene expression; gene expression regulation; genes; iron; phenotype; sequence analysis; swimming; transcriptome; transcriptomics; virulence
- Abstract:
- ... Escherichia coli is a leading cause of intramammary infections in dairy cattle and is typically transient in nature. However, in a minority of cases, E. coli can cause persistent infections. Although the mechanisms that allow for a persistent intramammary E. coli infection are not fully understood, we have previously shown that proficient bacterial motility (swimming and swarming) in vitro is corr ...
- Handle:
- 10113/5498088
- DOI:
- 10.1128/mSystems.00064-16
- PubMed:
- 27822550
- PubMed Central:
- PMC5072449
- https://doi.org/10.1128/mSystems.00064-16
- Author:
- John D. Lippolis, et al. ; Timothy A. Reinhardt; Brian J. Nonnecke; Randy E. Sacco; Show all 4 Authors
- Source:
- Journal of proteomics 2012 v.75 no.5 pp. 1486-1492
- ISSN:
- 1874-3919
- Subject:
- milk secretion; xanthine oxidase; fluid milk; chromatography; milk; dairy cows; proteome; trypsin; lactadherin; protein composition; lactation stage; adipophilin; milk fat; fat globules; spectrometers; exosomes; milk production; pH; butyrophilin
- Abstract:
- ... Exosomes are 40–100nm membrane vesicles of endocytic origin, secreted by cells and are found in biological fluids including milk. These exosomes are extracellular organelles important in intracellular communication, and immune function. Therefore, the proteome of bovine milk exosomes may provide insight into the complex processes of milk production. Exosomes were isolated from the milk of mid-lact ...
- Handle:
- 10113/54045
- DOI:
- 10.1016/j.jprot.2011.11.017
- https://doi.org/10.1016/j.jprot.2011.11.017
- Author:
- John D. Lippolis, et al. ; Timothy A. Reinhardt; Randy E. Sacco; Brian J. Nonnecke; Show all 4 Authors
- Source:
- Journal of Proteomics 2013 v.82 pp. 141-154
- ISSN:
- 1874-3919
- Subject:
- Staphylococcus aureus; proteome; protein synthesis; milk proteins; exosomes; mammary gland function; neutrophils; milk; whey; milk fat; cows; bovine mastitis; protein composition
- Abstract:
- ... Milk protein expression in healthy cows and cows with mastitis will provide information important for the dairy food industry and immune function in the mammary gland. To facilitate protein discovery, milk was fractioned into whey, milk fat globule membranes (MFGM) and exosomes from healthy and Staphylococcus aureus infected cows. Amine-reactive isobaric tags (iTRAQ) were used to quantify protein ...
- Handle:
- 10113/61334
- DOI:
- 10.1016/j.jprot.2013.02.013
- https://doi.org/10.1016/j.jprot.2013.02.013
- Author:
- John D. Lippolis, et al. ; Brian W. Brunelle; Timothy A. Reinhardt; Randy E. Sacco; Brian J. Nonnecke; Belgin Dogan; Kenneth Simpson; Ynte H. Schukken; Show all 8 Authors
- Source:
- Journal of proteomics 2014 v.108 pp. 373-381
- ISSN:
- 1874-3919
- Subject:
- Escherichia coli; assays; bacterial motility; chronic diseases; dairy cattle; infection; mastitis; phenotype; protein synthesis; proteins; proteomics; strains; swarming
- Abstract:
- ... Escherichia coli is a leading cause of bacterial mastitis in dairy cattle. Typically this infection is transient in nature, causing an infection that lasts 2–3 days. However, in a minority of cases, E. coli has been shown to cause a persistent infection. The mechanisms that allow for a persistent E. coli infection are not fully understood. The goal of this work was to determine protein expression ...
- Handle:
- 10113/61261
- DOI:
- 10.1016/j.jprot.2014.06.008
- https://doi.org/10.1016/j.jprot.2014.06.008
- Author:
- John D. Lippolis, et al. ; Corwin D. Nelson; Timothy A. Reinhardt; Randy E. Sacco; Jessi L. Powell; Mary E. Drewnoski; Matthew O’Neil; Donald C. Beitz; William P. Weiss; Show all 9 Authors
- Source:
- Journal of dairy science 2016 v.99 no.12 pp. 10150-10160
- ISSN:
- 0022-0302
- Subject:
- blood serum; calves; cholecalciferol; cows; dairy cattle; dairy industry; early lactation; heifers; herds; late lactation; metabolites; milk; milk replacer; neonates; nutritionists; pasteurization; statistical analysis; summer; vitamin status; vitamin supplements; wastes; yearlings; United States
- Abstract:
- ... The need for vitamin D supplementation of dairy cattle has been known for the better part of the last century and is well appreciated by dairy producers and nutritionists. Whether current recommendations and practices for supplemental vitamin D are meeting the needs of dairy cattle, however, is not well known. The vitamin D status of animals is reliably indicated by the concentration of the 25-hyd ...
- Handle:
- 10113/5564784
- DOI:
- 10.3168/jds.2016-11727
- https://doi.org/10.3168/jds.2016-11727
- Author:
- John D. Lippolis, et al. ; Corwin D. Nelson; Timothy A. Reinhardt; Donald C. Beitz; Derya Unutmaz; Show all 5 Authors
- Source:
- PloS one v.5 no.11 pp. -
- ISSN:
- 1932-6203
- Subject:
- dairy cows; innate immunity; 25-hydroxycholecalciferol; mammary glands; oxygenases; macrophages; gene expression; calcitriol; genes; Streptococcus uberis; in vitro studies; enzyme activity; gene expression regulation; bovine mastitis; pathogens; Toll-like receptors
- Abstract:
- ... Numerous in vitro studies have shown that toll-like receptor signaling induces 25-hydroxyvitamin D 3 1α-hydroxylase (1α-OHase; CYP27B1) expression in macrophages from various species. 1α-OHase is the primary enzyme that converts 25-hydroxyvitamin D 3 to 1,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3). Subsequently, synthesis of 1,25(OH) 2 D 3 by 1α-OHase in macrophages has been shown to modulate innate ...
- Handle:
- 10113/47708
- DOI:
- 10.1371/journal.pone.0015469
- PubMed:
- 21124742
- PubMed Central:
- PMC2993969
- https://doi.org/10.1371/journal.pone.0015469
- Author:
- John D. Lippolis, et al. ; Mark V. Boggess; William J. Hurkman; Clifton K. Fagerquist; Steve P. Briggs; Aldrin V. Gomes; Pier Giorgio Righetti; Kumar Bala; Show all 8 Authors
- Source:
- Journal of proteomics 2013 v.93 pp. 20-39
- ISSN:
- 1874-3919
- Subject:
- animal production; biotechnology; crop production; food security; genomics; genotype; human population; humans; infrastructure; length; metabolomics; overweight; phenotype; politics; population growth; proteomics; scientists; starvation
- Abstract:
- ... Increase in the world population has called for the increased demand for agricultural productivity. Traditional methods to augment crop and animal production are facing exacerbating pressures in keeping up with population growth. This challenge has in turn led to the transformational change in the use of biotechnology tools to meet increased productivity for both plant and animal systems. Although ...
- Handle:
- 10113/61353
- DOI:
- 10.1016/j.jprot.2013.03.021
- https://doi.org/10.1016/j.jprot.2013.03.021
- Author:
- John D. Lippolis, et al. ; Timothy A. Reinhardt; Randy A. Sacco; Brian J. Nonnecke; Corwin D. Nelson; Jürgen Schauber; Show all 6 Authors
- Source:
- PloS one v.6 no.10 pp. -
- ISSN:
- 1932-6203
- Subject:
- 25-hydroxycholecalciferol; tuberculosis; risk; plate count; milk; mastitis; mammary glands; lactation; influenza; gene expression; cows; blood serum; antimicrobial proteins; calcitriol; infection; vitamin deficiencies; vitamin supplements
- Abstract:
- ... Deficiency of serum levels of 25-hydroxyvitamin D 3 has been correlated with increased risk of infectious diseases such as tuberculosis and influenza. A plausible reason for this association is that expression of genes encoding important antimicrobial proteins depends on concentrations of 1,25-dihydroxyvitamin D 3 produced by activated immune cells at sites of infection, and that synthesis of 1,25 ...
- Handle:
- 10113/54043
- DOI:
- 10.1371/journal.pone.0025479
- PubMed:
- 21991312
- PubMed Central:
- PMC3184989
- https://doi.org/10.1371/journal.pone.0025479
- Author:
- John D. Lippolis, et al. ; Timothy A. Reinhardt; Brian J. McCluskey; Jesse P. Goff; Ronald L. Horst; Show all 5 Authors
- Source:
- veterinary journal 2011 v.188 no.1 pp. 122-124
- ISSN:
- 1090-0233
- Subject:
- dairy cows; herds; hypocalcemia; disease prevalence; lactation number; parturition; animal age; blood serum; calcium; free fatty acids; energy balance; United States
- Abstract:
- ... The prevalence of subclinical hypocalcemia in the transition cow is unknown. Cows with subclinical hypocalcemia have no clinical signs of hypocalcemia but may be more susceptible to other diseases. The objective of this study was to determine the prevalence of subclinical hypocalcemia in the US dairy herds. As a part of the United States Department of Agriculture’s National Animal Health Monitorin ...
- Handle:
- 10113/49532
- DOI:
- 10.1016/j.tvjl.2010.03.025
- PubMed:
- 20434377
- https://doi.org/10.1016/j.tvjl.2010.03.025