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You searched for: Author "Joseph P Rinehart" Remove constraint Author: "Joseph P Rinehart"
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1. Vitrification of manually stage-selected embryos of Drosophila have significantly higher survival and emergence – Consequences for insect germplasm storage

Author:
Joseph P. Rinehart,   et al.   ; Korie M. DeBardlabon; Arun Rajamohan;     Show all 3 Authors
Source:
Cryobiology 2022 v.105 pp. 83-87
ISSN:
0011-2240
Subject:
Drosophila melanogaster; cryobiology; cryopreservation; eclosion; embryogenesis; flight; germplasm; insects; mortality; mutants; permeability; temperature; viability; vitrification
Abstract:
... Embryonic selection for vitrification and cryostorage in Drosophila and other dipterans is generally carried out by gross observation of the embryonic development at a constant temperature. In this study, the effect of embryo developmental temperature (19, 20 and 21 °C) on the stage specific convergence of the embryonic development to the developmental stages 15–17, which are relevant for cryopres ...
DOI:
10.1016/j.cryobiol.2022.01.002

2. Comparison of Fluctuating Thermal Regimes and Commercially Achievable Constant-Temperature Regimes for Short-Term Storage of the Alfalfa Leafcutting Bee (Hymenoptera: Megachilidae)

Author:
Joseph P. Rinehart,   et al.   ; George D. Yocum; Arun Rajamohan;     Show all 3 Authors
Source:
Journal of economic entomology 2021 v.114 no.2 pp. 530-537
ISSN:
0022-0493
Subject:
Megachile rotundata; cold storage; entomology; refrigeration; spring; storage time; temperature
Abstract:
... Interrupting the spring incubation of Megachile rotundata (F.) with a period of low-temperature storage for synchronizing the bees' emergence with crop bloom is an essential part of M. rotundata management. Previously, we demonstrated that bees exposed to thermoperiods (TPs) during low-temperature storage have higher survival rates than bees exposed to constant temperatures. But changing the tempe ...
DOI:
10.1093/jee/toab019

3. Size constrains oxygen delivery capacity within but not between bumble bee castes

Author:
Joseph P. Rinehart,   et al.   ; Rikki M. Walter; Michael E. Dillon; Kendra J. Greenlee;     Show all 4 Authors
Source:
Journal of insect physiology 2021 v.134 pp. 104297
ISSN:
0022-1910
Subject:
Bombus impatiens; flight; hypoxia; insect physiology; life history; metabolism; overwintering; oxygen; spring
Abstract:
... Bumble bees are eusocial, with distinct worker and queen castes that vary strikingly in size and life-history. The smaller workers rely on energetically-demanding foraging flights to collect resources for rearing brood. Queens can be 3 to 4 times larger than workers, flying only for short periods in fall and again in spring after overwintering underground. These differences between castes in size ...
DOI:
10.1016/j.jinsphys.2021.104297
CHORUS:
10.1016/j.jinsphys.2021.104297

4. Cryopreservation of seminal vesicle derived spermatozoa from Bombus impatiens and Apis mellifera – Implications for artificial insemination of bumble bees

Author:
Joseph P. Rinehart,   et al.   ; Claire Campion; Arun Rajamohan;     Show all 3 Authors
Source:
Cryobiology 2021 v.102 pp. 136-139
ISSN:
0011-2240
Subject:
Apis mellifera; Bombus impatiens; artificial insemination; cryobiology; cryopreservation; dimethyl sulfoxide; fluorescence; honey bees; liquid nitrogen; males; phosphates; semen; seminal vesicles; spermatozoa; storage time; testes; viability
Abstract:
... This study evaluates the efficacy of a cryopreservation protocol for spermatozoa derived from the accessory testis of male Bombus impatiens. It is also the first report of successful cryopreservation of bumble bee spermatozoa. The spermatozoa viability was compared with the similarly treated honey bee spermatozoa derived from its accessory testis. The semen was frozen using a yolk-free non-activat ...
DOI:
10.1016/j.cryobiol.2021.06.002

5. Fluctuating temperatures extend longevity in pupae and adult stages of the sepsid Themira biloba

Author:
Joseph P. Rinehart,   et al.   ; Dacotah Melicher; Julia H. Bowsher;     Show all 3 Authors
Source:
Journal of thermal biology 2021 v.99 pp. 102959
ISSN:
0306-4565
Subject:
Sepsidae; feces; insects; labor; larval development; longevity; mortality; pupae; pupation
Abstract:
... Fluctuating Thermal Regimes (FTR), where organisms are held at low temperatures with a brief, daily warm pulse, have been shown to increase longevity in adult insects and improve pupa survival while reducing sublethal effects. We used FTR to extend the longevity and thus generation time of the fly species Themira biloba (Diptera: Sepsidae). T. biloba can be maintained in continuous culture and req ...
DOI:
10.1016/j.jtherbio.2021.102959
CHORUS:
10.1016/j.jtherbio.2021.102959

6. Physiological responses to cryoprotectant treatment in an early larval stage of the malaria mosquito, Anopheles gambiae

Author:
Joseph P. Rinehart,   et al.   ; Jacob B. Campbell; Andrew Dosch; Catherine M. Hunt; Ellen M. Dotson; Mark Q. Benedict; Arun Rajamohan;     Show all 7 Authors
Source:
Cryobiology 2021 v.99 pp. 114-121
ISSN:
0011-2240
Subject:
Anopheles gambiae; cryobiology; cryopreservation; cryoprotectants; dimethyl sulfoxide; ethane; glass; instars; malaria; mortality; permeability; temperature; toxicity; trehalose; viability; water content
Abstract:
... The development of cryopreservation protocols for Anopheles gambiae could significantly improve research and control efforts. Cryopreservation of any An. gambiae life stage has yet to be successful. The unique properties of embryos have proven to be resistant to any practical cryoprotectant loading. Therefore, we have chosen to investigate early non-feeding first instar larvae as a potential life ...
Handle:
10113/7197555
DOI:
10.1016/j.cryobiol.2020.12.001

7. A non-activating diluent to prolong in vitro viability of Apis mellifera spermatozoa: Effects on cryopreservation and on egg fertilization

Author:
Joseph P. Rinehart,   et al.   ; Arun Rajamohan; Robert G. Danka; Brandon K. Hopkins;     Show all 4 Authors
Source:
Cryobiology 2020 v.92 pp. 124-129
ISSN:
0011-2240
Subject:
Apis mellifera; cryobiology; cryopreservation; eggs; females; insemination; progeny; semen; spermatozoa; storage time; viability
Abstract:
... A non-activating semen diluent does not cause motility or acrosomal reaction or capacitate the sperm cell. The effects of such a diluent on the viability of honey bee spermatozoa stored in ambient conditions were assessed 60 days pre-cryopreservation and 24 h post-cryopreservation. Seven variations of a Tris-based non-activating diluents (FEM1 – FEM7) were compared to samples treated with conventi ...
DOI:
10.1016/j.cryobiol.2019.11.045
CHORUS:
10.1016/j.cryobiol.2019.11.045

8. Body size allometry impacts flight-related morphology and metabolic rates in the solitary bee Megachile rotundata

Author:
Joseph P. Rinehart,   et al.   ; Courtney C. Grula; Kendra J. Greenlee; Julia H. Bowsher;     Show all 4 Authors
Source:
Journal of insect physiology 2021 v.133 pp. 104275
ISSN:
0022-1910
Subject:
Megachile rotundata; adults; allometry; biomechanics; body size; carbon dioxide; diet; energy; flight; insect physiology; larvae; life history; metabolism; morphometry; pollination; power requirement; solitary bees; thorax
Abstract:
... Body size is related to many aspects of life history, including foraging distance and pollination efficiency. In solitary bees, manipulating the amount of larval diet produces intraspecific differences in adult body size. The goal of this study was to determine how body size impacts metabolic rates, allometry, and flight-related morphometrics in the alfalfa leafcutting bee, Megachile rotundata. By ...
Handle:
10113/7435856
DOI:
10.1016/j.jinsphys.2021.104275

9. Microclimate Temperatures Impact Nesting Preference in Megachile rotundata (Hymenoptera: Megachilidae)

Author:
Joseph P. Rinehart,   et al.   ; Elisabeth S. Wilson; Claire E. Murphy; George Yocum; Julia H. Bowsher;     Show all 5 Authors
Source:
Environmental entomology 2020 v.49 no.2 pp. 296-303
ISSN:
0046-225X
Subject:
Megachile rotundata; alfalfa; females; foraging; microclimate; nest boxes; nesting; nests; progeny; solitary bees; temperature; three-dimensional printing; North Dakota
Abstract:
... The temperature of the nest influences fitness in cavity-nesting bees. Females may choose nest cavities that mitigate their offspring's exposure to stressful temperatures.This study aims to understand how cavity temperature impacts the nesting preference of the solitary bee Megachile rotundata (Fabricius) under field conditions. We designed and 3D printed nest boxes that measured the temperatures ...
DOI:
10.1093/ee/nvaa012

10. Fluctuating thermal regimes prevent chill injury but do not change patterns of oxidative stress in the alfalfa leafcutting bee, Megachile rotundata

Author:
Joseph P. Rinehart,   et al.   ; Alex S. Torson; George D. Yocum; Sean A. Nash; Julia H. Bowsher;     Show all 5 Authors
Source:
Journal of insect physiology 2019 pp. -
ISSN:
0022-1910
Subject:
Megachile rotundata; antioxidant activity; apolipoprotein D; chilling injury; cold stress; cold treatment; epithelium; gene expression; gene expression regulation; glutathione peroxidase; glutathione transferase; insects; lipid peroxidation; messenger RNA; overwintering; oxidative stress; prepupae; pupal development; reactive oxygen species; stress response; temperature; transcriptomics; vitellogenin
Abstract:
... In insects, prolonged exposure to unseasonably low temperatures can lead to detrimental physiological effects known as chill injury. Changes to active and passive transport across epithelia during chilling likely drive the collapse of ion gradients, metabolic imbalance and potentially oxidative stress. In the alfalfa leafcutting bee, Megachile rotundata transcriptomic evidence provides support for ...
DOI:
10.1016/j.jinsphys.2019.103935

11. Long-Distance Transportation Causes Temperature Stress in the Honey Bee, Apis mellifera (Hymenoptera: Apidae)

Author:
Joseph P Rinehart,   et al.   ; Dacotah Melicher; Elisabeth S Wilson; Julia H Bowsher; Steve S Peterson; George D Yocum;     Show all 6 Authors
Source:
Environmental entomology 2019 v.48 no.3 pp. 691-701
ISSN:
1938-2936
Subject:
Apis mellifera; air flow; antimicrobial peptides; crops; economic impact; gene expression; heat stress; honey bees; methylation; pheromones; pollination; protein folding; shipping; temperature; thermoregulation; transcription (genetics); transportation
Abstract:
... Pollination services provided by the honey bee, Apis mellifera (Hymenoptera: Apidae, Linnaeus, 1758) have broad economic impacts and are necessary for production of a diversity of important crops. Hives may be transported multiple times per year to provide pollination. To test how temperature may contribute to transportation stress, temperature sensors were placed in hives in different locations a ...
Datasets:
Data from: Long-Distance Transportation Causes Temperature Stress in the Honey Bee, Apis mellifera (Hymenoptera: Apidae)
Handle:
10113/6734852
DOI:
10.1093/ee/nvz027
PubMed:
30927358
PubMed Central:
PMC6554651

12. Thermoprofile Parameters Affect Survival of Megachile rotundata During Exposure to Low-Temperatures

Author:
Joseph P. Rinehart,   et al.   ; George D. Yocum; Arun Rajamohan; Julia H. Bowsher; Kathleen M. Yeater; Kendra J. Greenlee;     Show all 6 Authors
Source:
Integrative and comparative biology 2019 v.59 no.4 pp. 1089-1102
ISSN:
1540-7063
Subject:
Megachile rotundata; chilling injury; cold stress; temperature
Abstract:
... Insects exposed to low temperature stress can experience chill injury, but incorporating fluctuating thermos profiles increases survival and blocks the development of sub-lethal effects. The specific parameters required for a protective thermoprofile are poorly understood, because most studies test a limited range of thermoprofiles. For example, thermoprofiles with a wave profile may perform bette ...
Handle:
10113/6759788
DOI:
10.1093/icb/icz126

13. Effect of cryopreservation on the pre-hatching behavior in the Mexican fruit fly Anastrepha ludens Loew (Diptera, Tephritidae)

Author:
Joseph P. Rinehart,   et al.   ; Arun Rajamohan; Roger A. Leopold;     Show all 3 Authors
Source:
Cryobiology 2018 v.80 pp. 38-42
ISSN:
0011-2240
Subject:
Anastrepha ludens; cryopreservation; eclosion; eggs; embryogenesis; hatching; larvae; permeability; pests; toxicity; vitrification
Abstract:
... In a sampling of untreated embryos of the economically important fruit pest species, Anastrepha ludens, the cumulative hatch percentage in the lab was noted to be ∼85%. Approximately 70% of the larvae had eclosed through the posterior pole of the egg. This process is effected by the act of Pole Reversal (PR) of the fully developed pre-hatch larva from the wider anterior to the narrower posterior p ...
Handle:
10113/5922783
DOI:
10.1016/j.cryobiol.2017.12.007

14. Cues for cavity nesters: investigating relevant zeitgebers for emerging leafcutting bees, Megachile rotundata

Author:
Joseph P. Rinehart,   et al.   ; Meghan M. Bennett; George D. Yocum; Curt Doetkott; Kendra J. Greenlee;     Show all 5 Authors
Source:
Journal of experimental biology 2018 v.221 no.10 pp. 1-10
ISSN:
1477-9145
Subject:
Megachile rotundata; air temperature; bees; brood cells; circadian clocks; circadian rhythm; eclosion; nesting; nesting sites; photoperiod
Abstract:
... Photoperiod is considered the universal zeitgeber, regulating physiological processes in numerous animals. However, for animals in light-restricted habitats (e.g. burrows or cavities), thermoperiod may be a more important cue. Our study tested this hypothesis in the alfalfa leafcutting bee, Megachile rotundata, which nests in cavities and undergoes development within a brood cell. We assessed the ...
Handle:
10113/6472293
DOI:
10.1242/jeb.175406
CHORUS:
10.1242/jeb.175406
Chorus Open Access:
10.1242/jeb.175406

15. Micro-computed tomography of pupal metamorphosis in the solitary bee Megachile rotundata

Author:
Joseph P. Rinehart,   et al.   ; Bryan R. Helm; Scott Payne; George D. Yocum; Julia H. Bowsher; Kendra J. Greenlee;     Show all 6 Authors
Source:
Arthropod structure & development 2018 v.47 no.5 pp. 521-528
ISSN:
1467-8039
Subject:
Megachile rotundata; adults; arthropods; brain; digestive system; fat body; flight muscles; metamorphosis; micro-computed tomography; pollinators; prepupae; pupae; solitary bees
Abstract:
... Insect metamorphosis involves a complex change in form and function. In this study, we examined the development of the solitary bee, Megachile rotundata, using micro-computed tomography (μCT) and volume analysis. We describe volumetric changes of brain, tracheae, flight muscles, gut, and fat bodies in prepupal, pupal, and adult M. rotundata. We observed that individual organ systems have distinct ...
Handle:
10113/6147879
DOI:
10.1016/j.asd.2018.05.001

16. Metamorphosis is induced by food absence rather than a critical weight in the solitary bee, Osmia lignaria

Author:
Joseph P. Rinehart,   et al.   ; Bryan R. Helm; George D. Yocum; Kendra J. Greenlee; Julia H. Bowsher;     Show all 5 Authors
Source:
Proceedings of the National Academy of Sciences 2017 v.114 no.41 pp. 10924-10929
ISSN:
1091-6490
Subject:
Osmia lignaria; adults; body size; economic valuation; insect larvae; juvenile hormones; juveniles; phenotype; phenotypic variation; pollinators; pupation; solitary bees; starvation
Abstract:
... Body size is an important phenotypic trait that correlates with performance and fitness. For determinate growing insects, body size variation is determined by growth rate and the mechanisms that stop growth at the end of juvenile growth. Endocrine mechanisms regulate growth cessation, and their relative timing along development shapes phenotypic variation in body size and development time. Larval ...
Handle:
10113/5852199
DOI:
10.1073/pnas.1703008114
PubMed:
28973885
PubMed Central:
PMC5642682

17. Environmental history impacts gene expression during diapause development in the alfalfa leafcutting bee, Megachile rotundata

Author:
Joseph P. Rinehart,   et al.   ; George D. Yocum; Anna K. Childers; Arun Rajamohan; Theresa L. Pitts-Singer; Kendra J. Greenlee; Julia H. Bowsher;     Show all 7 Authors
Source:
Journal of Experimental Biology pp. -
ISSN:
0022-0949
Subject:
Megachile rotundata; RNA; alfalfa; autumn; cluster analysis; diapause; gene expression; gene expression regulation; laboratory rearing; prepupae; principal component analysis; sequence analysis
Abstract:
... Our understanding of the mechanisms controlling insect diapause has increased dramatically with the introduction of global gene expression techniques, such as RNA sequencing (RNA-seq). However, little attention has been given to how ecologically relevant field conditions may affect gene expression during diapause development because previous studies have focused on laboratory-reared and -maintaine ...
Handle:
10113/7405987
DOI:
10.1242/jeb.173443
CHORUS:
10.1242/jeb.173443

18. Stage selection and restricted oviposition period improves cryopreservation of dipteran embryos

Author:
Joseph P. Rinehart,   et al.   ; Arun Rajamohan; Roger A. Leopold;     Show all 3 Authors
Source:
Cryobiology 2015 v.70 no.2 pp. 143-149
ISSN:
0011-2240
Subject:
Lucilia sericata; Musca domestica; chorion; cryopreservation; developmental stages; egg hatchability; eggs; embryogenesis; hatching; insect development; oviposition; sampling; viability
Abstract:
... Embryos of two dipteran species (Musca domestica and Lucilia sericata) were assessed for an effective sampling time that would result in the highest post-cryopreservation hatch rate, with a primary goal to define species-specific egg collection periods and the effects of manual stage selection on post cryopreservation yield. The effects of the time taken to collect eggs on, (a) the proportion of e ...
Handle:
10113/61207
DOI:
10.1016/j.cryobiol.2015.01.004

19. Optimizing Fluctuating Thermal Regime Storage of Developing Megachile rotundata (Hymenoptera: Megachilidae)

Author:
Joseph P. Rinehart,   et al.   ; George D. Yocum; William P. Kemp; Julia H. Bowsher;     Show all 4 Authors
Source:
Journal of economic entomology 2016 v.109 no.3 pp. 993-1000
ISSN:
0022-0493
Subject:
Megachile rotundata; adults; alfalfa; eclosion; managers; pollination; pollinators; pupae; spring; storage time; sublethal effects; survival rate; temperature; weather; North America
Abstract:
... The alfalfa leafcutting bee, Megachile rotundata (F.), is the primary pollinator for alfalfa seed production in North America. Under current management practice, developing pupae are incubated at 29–30C until the adults emerge for pollination. If unfavorable spring weather delays peak alfalfa bloom, managers will cool pupae to slow development, which can increase mortality and causes sublethal eff ...
Handle:
10113/63015
DOI:
10.1093/jee/tow026

20. Physiological responses to fluctuating temperatures are characterized by distinct transcriptional profiles in a solitary bee

Author:
Joseph P. Rinehart,   et al.   ; Alex S. Torson; George D. Yocum; Sean A. Nash; Kally M. Kvidera; Julia H. Bowsher;     Show all 6 Authors
Source:
Journal of Experimental Biology 2017 v.220 no.18 pp. 3372-3380
ISSN:
0022-0949
Subject:
Megachile rotundata; cell membranes; chilling injury; cold stress; cold treatment; diapause; gene expression regulation; homeostasis; mortality; oxidative stress; physiological response; pupae; pupal development; quantitative polymerase chain reaction; sequence analysis; solitary bees; temperature; transcription (genetics)
Abstract:
... Exposure to stressful low temperatures during development can result in the accumulation of deleterious physiological effects called chill injury. Metabolic imbalances, disruptions in ion homeostasis and oxidative stress contribute to the increased mortality of chill-injured insects. Interestingly, survival can be significantly increased when chill-susceptible insects are exposed to a daily warm-t ...
Handle:
10113/5852153
DOI:
10.1242/jeb.156695
CHORUS:
10.1242/jeb.156695
Chorus Open Access:
10.1242/jeb.156695