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... Ruminants have evolved with the capability to recycle endogenous urea to the gastrointestinal tract (GIT). Ruminal ammonia derived from urea recycling makes a net contribution to digestible N flow if it is used to synthesise microbial protein. The dynamics of urea recycling and its quantitative importance to the N economy of ruminants are affected by dietary and physiological factors. In general, ...
Holstein; ad libitum feeding; alfalfa hay; amino acid composition; casein; catabolism; corn silage; digestible protein; energy; essential amino acids; fistulated animals; galactose; glucose; jugular vein; lactose; mammary glands; milk; milk protein yield; standard deviation; total mixed rations
Abstract:
... This study investigated mammary gland metabolism and whole-body (WB) rate of appearance (Ra) of glucose in dairy cattle in response to a constant supplemental level of metabolizable protein (MP) composed of different essential AA (EAA) profiles. Five multiparous rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were abomasally infuse ...
data collection; models; moderate resolution imaging spectroradiometer; reflectance; remote sensing
Abstract:
... Linear kernel driven models of the surface Bidirectional Reflectance Distribution Function (BRDF) are valuable tools for exploiting Earth observation data acquired at different sun–sensor geometries. Here we present a method that efficiently determines linear BRDF model weights using Tikhonov smoothing where the smoothing parameter λ is determined via a Generalized Singular Value Decomposition wit ...
Holstein; ad libitum feeding; alfalfa hay; amino acid composition; body protein; casein; cattle feeds; climate; corn silage; dairy cows; digestibility; dry matter intake; energy; essential amino acids; lactose; milk; milk fat; milk protein yield; nitrogen balance; protein content; standard deviation; total mixed rations; total nitrogen; urea; urine
Abstract:
... Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear. This study determined the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning in dairy cattle. Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in ...
... Mammary gland utilization of AA and other metabolites in response to supplemental energy from protein (PT) and supplemental energy from fat (FT) was tested in a 2 × 2 factorial arrangement using a randomized complete block design. Fifty-six Holstein-Friesian dairy cows were adapted during a 28-d control period to a basal total mixed ration consisting of 34% grass silage, 33% corn silage, 5% grass ...
... Rumen sensors provide specific information to help understand rumen functioning in relation to health disorders and to assist in decision-making for farm management. This review focuses on the use of rumen sensors to measure ruminal pH and discusses variation in pH in both time and location, pH-associated disorders and data analysis methods to summarize and interpret rumen pH data. Discussion on t ...
3-hydroxybutyric acid; Holstein; ad libitum feeding; blood sampling; corn silage; dairy cows; digestible energy; energy; free fatty acids; glucose; grass silage; insulin; lactating females; lactose; lipogenesis; long chain fatty acids; mammary glands; milk; milk proteins; milk yield; palm oils; protein content; protein requirement; total mixed rations; triacylglycerols; urea
Abstract:
... We investigated mammary gland metabolism in lactating dairy cattle in response to energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels. According to a 6 × 6 Latin square design, 6 rumen-fistulated second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were abomasally infused with saline (LMP-C); isoenerget ...
... This study tested the effects of energy from glucogenic (glucose; GG) or lipogenic (palm olein; LG) substrates at low (LMP) and high (HMP) metabolizable protein levels on whole-body energy and N partitioning of dairy cattle. Six rumen-fistulated, second-lactation Holstein-Friesian dairy cows (97 ± 13 d in milk) were randomly assigned to a 6 × 6 Latin square design in which each experimental period ...
... Secretory capacity of bovine mammary glands is enabled by a high number of secretory cells and their ability to use a range of metabolites to produce milk components. We isolated RNA from milk fat to measure expression of genes involved in energy-yielding pathways and the unfolded protein response in mammary glands of lactating cows given supplemental energy from protein (PT) and fat (FT) tested i ...
... Fifty-six Holstein-Friesian cows were used in a randomized complete block design to test the effects of supplemental energy from protein (PT) and fat (FT) on lactation performance and nutrient digestibility in a 2 × 2 factorial arrangement. During the control period, cows were adapted for 28 d to a basal total mixed ration consisting of 34% grass silage, 33% corn silage, 5% grass hay, and 28% conc ...
electrospray ionization mass spectrometry; humans; ions; isomers; lipids; molecular weight; tandem mass spectrometry; wax esters
Abstract:
... Wax esters (WE) are one of the predominant lipid types in human meibomian gland secretions (meibum) and account for 40–50 % of total meibum lipids. Recently, we managed to quantify 51 isomeric groups of intact WE in normal human meibum samples by direct infusion electrospray ionization mass spectrometry (ESI–MS), with each WE peak in the MS spectrum corresponding to one isomeric group (Chen et al, ...
... Core Ideas Information about forage‐induced changes to soil properties is lacking. Soil responses to perennial forages were quantified in a semiarid region over 5 yr. Perennial forages maintained or improved near‐surface soil conditions. However, effects were subtle and changes to soil properties occurred slowly. Integration of perennial forages in annual cropping systems can expand ecosystem serv ...
... The phosphorylation of mammalian target of rapamycin complex 1 (mTORC1) components and integrated stress response networks in the mammary glands of lactating cows have not accounted for the stimulation of milk protein yield by chronic supplementation with AA or glucose. Faster milk protein synthesis could be a consequence of increased milk protein mRNA per cell, the number of ribosomes per cell, t ...
arithmetics; basins; climatic factors; data collection; geophysics; grains; gravel; humans; land use; landscapes; landslides; quartz; regression analysis; rivers; sand; sediment yield; silicates; soil creep; streams; tectonics; temperature; topography; tropics; watersheds; weathering; Australia; Madagascar; Panama; Puerto Rico; Sri Lanka
Abstract:
... Erosion rates of tropical landscapes are poorly known. Using measurements of in situ-produced 10Be in quartz extracted from river and landslide sediment samples, we calculate long-term erosion rates for many physiographic regions of Panama. We collected river sediment samples from a wide variety of watersheds (n=35), and then quantified 24 landscape-scale variables (physiographic, climatic, seismi ...
Holstein; amino acid metabolism; chemical structure; crude protein; dairy cows; dairy protein; early lactation; energy; essential amino acids; excretion; gene expression; glucose; messenger RNA; milk; milk fat; milk fat yield; milk protein yield; muscle protein; muscles; protein degradation; protein synthesis; tissues; urea nitrogen
Abstract:
... To determine how glucose modulates protein synthesis when essential AA are in abundant supply, 5 early-lactation, rumen-fistulated Holstein dairy cows were fed a diet containing 6.95 MJ/kg of net energy for lactation and 12.4% crude protein and abomasally infused for 5d with saline, 844 or 1,126g/d of a complete essential AA mix, with and without the inclusion of 1,000g/d of glucose, in a 5×5 Lati ...
... In decadal prediction, the objective is to exploit both the sources of predictability from the external radiative forcings and from the internal variability to provide the best possible climate information for the next decade. Predicting the climate system internal variability relies on initialising the climate model from observational estimates. We present a refined method of anomaly initialisati ...
ammonia; dissociation; electrospray ionization mass spectrometry; energy; fatty acids; humans; ionization; ions; methanol; spectrometers; tandem mass spectrometry; wax esters
Abstract:
... A series of different types of wax esters (represented by RCOOR′) were systematically studied by using electrospray ionization (ESI) collision-induced dissociation tandem mass spectrometry (MS/MS) along with pseudo MS³ (in-source dissociation combined with MS/MS) on a quadrupole time-of-flight (Q-TOF) mass spectrometer. The tandem mass spectra patterns resulting from dissociation of ammonium/proto ...
... Perennial herbaceous biofeedstocks (PHB) have been proposed to confer multiple ecosystem services to agricultural lands. However, the role of PHBs to affect change in soil condition is not well documented, particularly for treatments with multiple species. The objective of this study was to quantify potential changes to soil properties resulting from PHB treatments in central and western North Dak ...
... Estimates of long-term, background sediment generation rates place current and future sediment fluxes to the Great Barrier Reef in context. Without reliable estimates of sediment generation rates and without identification of the sources of sediment delivered to the reef prior to European settlement (c. 1850), determining the necessity and effectiveness of contemporary landscape management efforts ...
... The ongoing invasion of non-native Indo-Pacific lionfish (Pterois spp.) represents a significant ecological threat throughout the Western Atlantic and Caribbean. As a generalist species, lionfish have been able to rapidly colonize a wide variety of ecosystems, including coral reefs, seagrass beds, mangroves, the sea floor at depths as great as 300 m, and even brackish estuaries. While lionfish hav ...